FastQCFastQC Report
Wed 25 May 2016
SRR1033136_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033136_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3267680
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58600.179332125544729No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48250.14765827743230672No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43750.049.251231
GTATCAA83450.041.3808481
TCAACGC94750.035.474044
ATCAACG95200.035.4051173
CAACGCA96750.034.644085
AACGCAG100400.033.46756
TATCAAC105250.032.2477152
ACGCAGA115550.028.8761527
CGCAGAG115900.028.6263228
GTACATG90250.028.1498571
TACATGG92100.026.1843362
GCAGAGT130550.025.0175939
ACATGGG94200.024.3530163
TCGACCG2002.2962922E-823.5050145
GAGTACT86950.021.42913212-13
AGAGTAC117650.021.30983510-11
GTCGTAC2458.411007E-921.1230321
CAGAGTA129850.020.28482410-11
GTACTTT95850.019.26776714-15
CATGGGG62250.019.2568244
Produced by FastQC (version 0.11.5)