##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033136_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3267680
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.75755122900651	34.0	31.0	34.0	31.0	34.0
2	32.861379633256625	34.0	31.0	34.0	31.0	34.0
3	32.95842463154287	34.0	31.0	34.0	31.0	34.0
4	36.3764643416736	37.0	37.0	37.0	35.0	37.0
5	36.3161836532341	37.0	37.0	37.0	35.0	37.0
6	36.398305525632864	37.0	37.0	37.0	35.0	37.0
7	36.36920322675415	37.0	37.0	37.0	35.0	37.0
8	36.37035817460706	37.0	37.0	37.0	35.0	37.0
9	38.18952069970132	39.0	39.0	39.0	37.0	39.0
10-11	38.17183215614748	39.0	39.0	39.0	37.0	39.0
12-13	38.13247334500318	39.0	39.0	39.0	37.0	39.0
14-15	39.673352806884395	41.0	40.0	41.0	37.0	41.0
16-17	39.68712909464819	41.0	40.0	41.0	37.0	41.0
18-19	39.69807539293933	41.0	40.0	41.0	37.0	41.0
20-21	39.69190878543799	41.0	40.0	41.0	37.0	41.0
22-23	39.62485478994271	41.0	40.0	41.0	37.0	41.0
24-25	39.57619794471919	41.0	40.0	41.0	37.0	41.0
26-27	39.473715908534494	41.0	39.5	41.0	36.5	41.0
28-29	39.38834417690839	41.0	39.0	41.0	36.0	41.0
30-31	39.3012150822602	41.0	39.0	41.0	36.0	41.0
32-33	39.34302884615384	41.0	39.0	41.0	36.0	41.0
34-35	39.291363444400915	41.0	39.0	41.0	35.5	41.0
36-37	39.21074860451452	41.0	39.0	41.0	35.0	41.0
38-39	39.13048079983352	41.0	39.0	41.0	35.0	41.0
40-41	39.0241487844587	41.0	39.0	41.0	35.0	41.0
42-43	38.91918746633697	41.0	39.0	41.0	35.0	41.0
44-45	38.78638116339421	41.0	39.0	41.0	35.0	41.0
46-47	38.59041108676492	41.0	39.0	41.0	35.0	41.0
48-49	38.41505410566518	41.0	39.0	41.0	35.0	41.0
50-51	38.28893771727954	41.0	38.0	41.0	34.5	41.0
52-53	38.159695716838854	40.0	38.0	41.0	34.0	41.0
54-55	38.02235500416197	40.0	38.0	41.0	34.0	41.0
56-57	37.863931443715416	40.0	37.0	41.0	34.0	41.0
58-59	37.677455870831906	40.0	37.0	41.0	34.0	41.0
60-61	37.486803481369044	40.0	36.5	41.0	33.0	41.0
62-63	37.25207333643441	39.5	36.0	41.0	33.0	41.0
64-65	37.002432612740535	39.0	35.5	41.0	33.0	41.0
66-67	36.72327828918376	39.0	35.0	41.0	33.0	41.0
68-69	36.40369635337609	38.5	35.0	40.5	32.0	41.0
70-71	36.06797039489791	37.0	35.0	40.0	32.0	41.0
72-73	35.71678989374725	37.0	35.0	39.5	32.0	41.0
74-75	35.3809234686383	36.0	35.0	39.0	31.0	41.0
76-77	35.041036607011705	36.0	35.0	39.0	31.0	40.5
78-79	34.69825059369339	35.5	35.0	37.5	31.0	39.5
80-81	34.36227170347158	35.0	35.0	37.0	31.0	39.0
82-83	34.03988456642021	35.0	34.5	37.0	31.0	39.0
84-85	33.76572614209469	35.0	34.0	36.0	31.0	38.0
86-87	33.53124189027077	35.0	34.0	36.0	30.0	37.0
88-89	33.31173753243891	35.0	34.0	36.0	30.0	37.0
90-91	33.112284862654846	35.0	34.0	35.0	30.0	36.5
92-93	32.9485143894139	35.0	34.0	35.0	30.0	36.0
94-95	32.800151636635164	35.0	34.0	35.0	30.0	36.0
96-97	32.676581397199236	35.0	34.0	35.0	30.0	36.0
98-99	32.56929748322969	35.0	34.0	35.0	30.0	36.0
100	32.471692148558	35.0	34.0	35.0	29.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.13211918700365288
1101	2	0.12234812508462056
1101	3	0.09128804976518978
1101	4	0.10586318191191424
1101	5	0.09279769746538591
1101	6	0.11516342574133631
1101	7	0.12493452290895135
1101	8	0.10876746982039975
1101	9	0.13225942597976115
1101	10-11	0.10686434082669649
1101	12-13	0.1467953126326833
1101	14-15	0.1551547191151812
1101	16-17	0.17756515717429266
1101	18-19	0.22660694896474354
1101	20-21	0.2107506675334676
1101	22-23	0.2089155916250789
1101	24-25	0.16173082251537352
1101	26-27	0.18963880081513196
1101	28-29	0.1690063738368366
1101	30-31	0.15011070116292302
1101	32-33	0.11572806341691688
1101	34-35	0.18653459395899574
1101	36-37	0.1577221520105212
1101	38-39	0.14000403564570973
1101	40-41	0.12929821815308884
1101	42-43	0.13785526316947028
1101	44-45	0.06650615329704124
1101	46-47	0.01693299929844727
1101	48-49	-0.007660739827670682
1101	50-51	-0.04639226012223929
1101	52-53	0.008387422199440664
1101	54-55	-0.007861066002064376
1101	56-57	0.06736177449429448
1101	58-59	-0.007863725267917232
1101	60-61	0.012263696851597672
1101	62-63	-0.02178646527649164
1101	64-65	0.006140026630873763
1101	66-67	0.03171944070415833
1101	68-69	0.019962478027196084
1101	70-71	2.043553390151942E-4
1101	72-73	0.06803044025803473
1101	74-75	0.0543195937828429
1101	76-77	0.015475420646673399
1101	78-79	-0.018117010925934096
1101	80-81	-0.06836050880374955
1101	82-83	0.021780948562479807
1101	84-85	0.06308426168163095
1101	86-87	0.00411293801648327
1101	88-89	0.006860217477530739
1101	90-91	0.00545328520119881
1101	92-93	-0.040672510788304805
1101	94-95	-0.012369790209788789
1101	96-97	0.07942127299842383
1101	98-99	0.10751474506320591
1101	100	0.07932925584037775
1102	1	0.1375129262687267
1102	2	0.17831829977920677
1102	3	0.1590641881572239
1102	4	0.1182594023983441
1102	5	0.11957553435542678
1102	6	0.1135635473770904
1102	7	0.12514379134312748
1102	8	0.15315950435373793
1102	9	0.20260045414475059
1102	10-11	0.17894543334330848
1102	12-13	0.20846253676409532
1102	14-15	0.2579557797852061
1102	16-17	0.19212041719019624
1102	18-19	0.19167229409256237
1102	20-21	0.21645599614968347
1102	22-23	0.2109871153583711
1102	24-25	0.21318733471333218
1102	26-27	0.22079768722849025
1102	28-29	0.23569716051277112
1102	30-31	0.24893532067618906
1102	32-33	0.23993766964161267
1102	34-35	0.2913954901090605
1102	36-37	0.2541265238847643
1102	38-39	0.2482407915398568
1102	40-41	0.2535515092242946
1102	42-43	0.2249374982128387
1102	44-45	0.2848189578369116
1102	46-47	0.3276308289261891
1102	48-49	0.26248286886986705
1102	50-51	0.2904632228081425
1102	52-53	0.2634318774966431
1102	54-55	0.26354779610947077
1102	56-57	0.3359964053471174
1102	58-59	0.3231760481046422
1102	60-61	0.32016886187122395
1102	62-63	0.29926579309858425
1102	64-65	0.27441266254042773
1102	66-67	0.2918169226842764
1102	68-69	0.3579976466175836
1102	70-71	0.38138615072873705
1102	72-73	0.3374492068005637
1102	74-75	0.3225096814038295
1102	76-77	0.2971171536717776
1102	78-79	0.26865859253371127
1102	80-81	0.2364359704606045
1102	82-83	0.19691305991490538
1102	84-85	0.30100727661200466
1102	86-87	0.32068840059371695
1102	88-89	0.2874544151558922
1102	90-91	0.2569868327282663
1102	92-93	0.2735668108722251
1102	94-95	0.19366718285209572
1102	96-97	0.2141062083399845
1102	98-99	0.2914026486756356
1102	100	0.32876680735391517
1103	1	0.12952279263728883
1103	2	0.133786953235159
1103	3	0.09742581782406035
1103	4	0.06643368002227135
1103	5	0.06212262578315375
1103	6	0.08777428629741024
1103	7	0.1207816495271814
1103	8	0.11938181807182957
1103	9	0.15368959675436145
1103	10-11	0.11658399407141928
1103	12-13	0.09791577677163588
1103	14-15	0.14462745974250169
1103	16-17	0.16517704098858133
1103	18-19	0.16742890044598369
1103	20-21	0.14240830781576364
1103	22-23	0.130308514933958
1103	24-25	0.12613997117079379
1103	26-27	0.1372244145026329
1103	28-29	0.14022296984789762
1103	30-31	0.12100154432818044
1103	32-33	0.14408757709220055
1103	34-35	0.1787508027639504
1103	36-37	0.23082245784220845
1103	38-39	0.1745290231011083
1103	40-41	0.18771230126726834
1103	42-43	0.09525621232752002
1103	44-45	0.1103034826003082
1103	46-47	0.12252583361203051
1103	48-49	0.1648651452834713
1103	50-51	0.09839271194794463
1103	52-53	0.08468305702464818
1103	54-55	0.0730015837634852
1103	56-57	0.08675404624933947
1103	58-59	0.13141796623785496
1103	60-61	0.09549604030355852
1103	62-63	0.025876305595076587
1103	64-65	0.04958570269212004
1103	66-67	0.1278808479294895
1103	68-69	0.0915208196566013
1103	70-71	0.11737907175735529
1103	72-73	0.14912710828760112
1103	74-75	0.06492857420122533
1103	76-77	0.047676545600054965
1103	78-79	0.07253151140590575
1103	80-81	0.11462795690250971
1103	82-83	0.11575555356097311
1103	84-85	0.12542882620666518
1103	86-87	0.14127609265201357
1103	88-89	0.13750971131494794
1103	90-91	0.12602073688577065
1103	92-93	0.11819926795221392
1103	94-95	0.1115039337082635
1103	96-97	0.12074294807236896
1103	98-99	0.0721844382248662
1103	100	0.034411951039459154
1104	1	0.043483170304092766
1104	2	0.07494746144442388
1104	3	0.08160491607793574
1104	4	0.046936929721859144
1104	5	-0.03071588721046936
1104	6	0.015230775572590005
1104	7	0.054780096350455665
1104	8	0.07054051133200545
1104	9	0.08976001623644692
1104	10-11	0.13721803859976944
1104	12-13	0.11614902762496371
1104	14-15	0.09171083926364787
1104	16-17	0.09980606164015171
1104	18-19	0.09397732722703722
1104	20-21	0.13306091294611377
1104	22-23	0.14594045935862
1104	24-25	0.11295970050412762
1104	26-27	0.10290367288178004
1104	28-29	0.13128935442634315
1104	30-31	0.061117699261068026
1104	32-33	0.06340901950139965
1104	34-35	0.0771402694922827
1104	36-37	0.09448258196338344
1104	38-39	0.06481679768452153
1104	40-41	0.04981056513481974
1104	42-43	0.07217128550913543
1104	44-45	0.1376872110954963
1104	46-47	0.1507644379546207
1104	48-49	0.03614565857802887
1104	50-51	0.04209077949525408
1104	52-53	0.044420765448123234
1104	54-55	-0.018973347235203164
1104	56-57	0.07844985137418803
1104	58-59	0.049273632603451745
1104	60-61	0.05226228404140443
1104	62-63	0.08212771977871114
1104	64-65	0.08754430240529842
1104	66-67	0.09542191030471514
1104	68-69	0.08311144999660058
1104	70-71	0.08230589285614798
1104	72-73	0.16478069370754866
1104	74-75	0.16592366198927522
1104	76-77	0.13419213414942277
1104	78-79	0.10268777476900226
1104	80-81	0.15385202342598348
1104	82-83	0.18231022977300881
1104	84-85	0.09384509384910444
1104	86-87	0.11571201683083387
1104	88-89	0.10232179217329929
1104	90-91	0.15649571308858867
1104	92-93	0.162379665118074
1104	94-95	0.08454235160813539
1104	96-97	0.10225755814120419
1104	98-99	0.09324863428134478
1104	100	0.1195996372633914
1105	1	0.03897972811288497
1105	2	0.05043307027129629
1105	3	0.08501770386870078
1105	4	0.07036932729340606
1105	5	0.05399521322284073
1105	6	0.07503805201203306
1105	7	0.007713764417893287
1105	8	-0.012967863963623927
1105	9	0.04325637640581448
1105	10-11	0.047337264022957015
1105	12-13	0.08443879006704691
1105	14-15	0.08642627962326088
1105	16-17	0.051350234442487874
1105	18-19	0.009508426033846717
1105	20-21	0.052934363085334724
1105	22-23	0.11776376277956757
1105	24-25	0.07239747695141574
1105	26-27	0.03148724513047085
1105	28-29	0.10461939867695946
1105	30-31	0.06458598112052982
1105	32-33	0.01284647463304367
1105	34-35	-0.0022029327636090557
1105	36-37	-0.04370838847826519
1105	38-39	-0.04901872889507786
1105	40-41	-0.037376246304603455
1105	42-43	-0.0069244560713315195
1105	44-45	0.028584204836946014
1105	46-47	-0.04074641588842809
1105	48-49	-0.13042293897621704
1105	50-51	-0.1231098015750618
1105	52-53	-0.07498400123039062
1105	54-55	-0.11951168950113811
1105	56-57	-0.09187744627728733
1105	58-59	-0.08465418951136172
1105	60-61	-0.12586707261107222
1105	62-63	-0.14488652004694558
1105	64-65	-0.23172167064108606
1105	66-67	-0.1975473008151596
1105	68-69	-0.2030060299291776
1105	70-71	-0.1421128138111598
1105	72-73	-0.19674605876395646
1105	74-75	-0.2562351781011145
1105	76-77	-0.1954939924373349
1105	78-79	-0.1933329605692009
1105	80-81	-0.19993076235287788
1105	82-83	-0.19573379202829244
1105	84-85	-0.20732556901119636
1105	86-87	-0.2255948665382661
1105	88-89	-0.21963976791025175
1105	90-91	-0.24728987012831283
1105	92-93	-0.19749601431077934
1105	94-95	-0.17384145325228673
1105	96-97	-0.22002434324018338
1105	98-99	-0.2435400402344925
1105	100	-0.272331867249882
1106	1	-0.004753552066013356
1106	2	0.013745017669883453
1106	3	-0.029287063654479084
1106	4	0.026346365517618153
1106	5	-0.0322793153335752
1106	6	-0.05352378162800875
1106	7	-0.041405345137960126
1106	8	0.04587642643139844
1106	9	-0.008622384505272862
1106	10-11	0.04379724249272954
1106	12-13	-0.0022745813603037845
1106	14-15	0.020879757077594263
1106	16-17	0.032988800400296725
1106	18-19	0.07191568262076942
1106	20-21	-0.009244327758544557
1106	22-23	0.08091186767635605
1106	24-25	0.062401867896902274
1106	26-27	0.02007997310727916
1106	28-29	0.04427913311955933
1106	30-31	0.08596402995012653
1106	32-33	0.05635454477810953
1106	34-35	0.0913699277368778
1106	36-37	0.07928317761779624
1106	38-39	0.06391082705063411
1106	40-41	0.05462635202103172
1106	42-43	0.08301731734044182
1106	44-45	0.03573517920067104
1106	46-47	0.03157725501583286
1106	48-49	0.008088782180450949
1106	50-51	-0.10333230019586637
1106	52-53	-0.0031411195620876242
1106	54-55	0.007879756661871795
1106	56-57	0.025871117814745048
1106	58-59	0.019847125255502363
1106	60-61	0.08965892977282408
1106	62-63	0.06144018307614374
1106	64-65	0.03405687402810287
1106	66-67	0.07891817095057263
1106	68-69	0.0843767383698335
1106	70-71	0.05792810067328702
1106	72-73	0.1139341820819979
1106	74-75	0.07164814791676832
1106	76-77	0.030664700393124633
1106	78-79	0.03474586906681765
1106	80-81	0.07185001352735298
1106	82-83	0.015058940579400826
1106	84-85	0.054602760758371005
1106	86-87	0.031203842251947833
1106	88-89	0.028444122135240946
1106	90-91	0.04679198434398302
1106	92-93	0.0034408745306890864
1106	94-95	-0.036728853101145376
1106	96-97	0.042250841021811425
1106	98-99	0.046189920812430785
1106	100	0.12031966651056791
1107	1	-0.018409451559563195
1107	2	-0.01466631111538419
1107	3	0.004702218886791343
1107	4	0.02361171363055803
1107	5	-0.12147425505087739
1107	6	-0.11075044874370832
1107	7	-0.032880571338395725
1107	8	0.0033728341663135097
1107	9	-0.008294349762941522
1107	10-11	-0.016244461459727688
1107	12-13	-0.0025261201006330225
1107	14-15	0.006045887310030196
1107	16-17	0.06325668073937862
1107	18-19	0.046584981744189236
1107	20-21	-0.0018898465682255505
1107	22-23	0.0688000808772884
1107	24-25	0.06734413907442871
1107	26-27	0.07619121384998806
1107	28-29	0.08767132988587889
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2216	3	-0.12344232197209237
2216	4	-0.0844977608456432
2216	5	-0.04676904116733027
2216	6	-0.031293923350972364
2216	7	-0.04877649398864037
2216	8	-0.05494132436103172
2216	9	-0.12670752804093155
2216	10-11	-0.13106834354053376
2216	12-13	-0.08010538190711003
2216	14-15	-0.2792249926870838
2216	16-17	-0.1921398345558174
2216	18-19	-0.12541435210319207
2216	20-21	-0.15428772438659877
2216	22-23	-0.1752808203368943
2216	24-25	-0.23296617192921332
2216	26-27	-0.27080850196563944
2216	28-29	-0.2631174600081607
2216	30-31	-0.22180903038108823
2216	32-33	-0.22874119970813211
2216	34-35	-0.2089683730510643
2216	36-37	-0.2733184789210483
2216	38-39	-0.29040935757836195
2216	40-41	-0.3004216137672273
2216	42-43	-0.266678411513368
2216	44-45	-0.1713114927544126
2216	46-47	-0.21093393714001252
2216	48-49	-0.21708693716085037
2216	50-51	-0.15654752967663654
2216	52-53	-0.07875344523552741
2216	54-55	-0.03778107944039277
2216	56-57	-0.06987731811057785
2216	58-59	-0.06769075013073689
2216	60-61	-0.009944085457149754
2216	62-63	-0.01923085568230931
2216	64-65	-0.03249238309878422
2216	66-67	0.022974853482502056
2216	68-69	0.02683566997628617
2216	70-71	0.023209239146694927
2216	72-73	0.04595461982334825
2216	74-75	0.07936291923424932
2216	76-77	0.05555851626969144
2216	78-79	0.026638623933656902
2216	80-81	0.09828514143057987
2216	82-83	0.0805409516418365
2216	84-85	0.06571207718872074
2216	86-87	0.03547958254736727
2216	88-89	0.014108849715171345
2216	90-91	0.004610819428478408
2216	92-93	0.011584810145023994
2216	94-95	-0.028990799438673776
2216	96-97	-0.020431052298484076
2216	98-99	0.03816676236277061
2216	100	0.052559727443785675
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	27.0
3	9.0
4	12.0
5	17.0
6	68.0
7	176.0
8	308.0
9	675.0
10	1198.0
11	1922.0
12	2915.0
13	4688.0
14	5347.0
15	7326.0
16	8875.0
17	12825.0
18	15321.0
19	8697.0
20	5992.0
21	5709.0
22	5865.0
23	6204.0
24	7252.0
25	8765.0
26	11217.0
27	14166.0
28	18120.0
29	23329.0
30	29955.0
31	38990.0
32	49274.0
33	64962.0
34	90789.0
35	140342.0
36	265288.0
37	625594.0
38	1172465.0
39	610331.0
40	2665.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.013286161715946	19.840657952169817	13.297954620291453	23.848101265822784
2	15.515455320268877	20.260340398054254	40.31037643318047	23.913827848496393
3	18.138103528528966	25.55027589919328	30.250309164394668	26.061311407883085
4	12.013449334403617	16.698743539358766	39.09772434250572	32.190082783731896
5	12.371088256058233	38.10292243834309	36.13211804375606	13.393871261842612
6	30.790026547927805	38.52381280268081	17.589233935443396	13.09692671394799
7	27.08857048246347	32.733061935007626	22.730986968106105	17.447380614422794
8	25.213990396827096	36.43822662631172	20.189125584896882	18.158657391964304
9	25.1929204558113	16.01909985959406	21.239764872073906	37.54821481252073
10-11	23.874873361425763	26.66859566868151	29.138309903507565	20.31822106638516
12-13	24.752484943446117	23.975526979385986	29.430023747735394	21.841964329432503
14-15	21.729311596376142	25.920669613511254	26.747602883517214	25.60241590659539
16-17	21.038672697448956	29.468307790236498	27.287938231405768	22.20508128090878
18-19	20.907446287123676	28.07212650093315	29.92690063624739	21.09352657569578
20-21	21.97709078000294	27.354958257846544	29.601796993585662	21.066153968564855
22-23	21.563699199537776	27.13816813761591	29.75580952141959	21.542323141426724
24-25	21.4942941873224	27.296107800144842	29.621077851494785	21.588520161037973
26-27	21.28441782024565	27.307455073194696	29.69980755109108	21.708319555468574
28-29	21.26202323120489	27.429777290468106	29.767756813770085	21.54044266455692
30-31	21.46135741187146	27.732806722462684	29.43831201234868	21.367523853317177
32-33	21.261746326489256	27.402743263521174	29.836403954247103	21.499106455742467
34-35	21.37195177949343	27.31133634966602	29.76618319629224	21.550528674548318
36-37	21.697527547962707	27.576217025019	29.28811356339024	21.438141863628058
38-39	21.494794268614026	27.731554948230432	29.351404929129078	21.422245854026468
40-41	21.366227899655236	27.492883990524643	29.502647724928043	21.63824038489208
42-43	21.401282152139782	27.48034728244416	29.37181708011782	21.74655348529823
44-45	21.47291347970587	27.68575947938293	29.221699214161468	21.61962782674973
46-47	21.67369149688187	27.822061849012037	28.925119100217735	21.579127553888362
48-49	21.648838962407105	27.78428503351245	28.75179850720459	21.815077496875855
50-51	21.621107946850625	27.81595123944304	28.592869855037424	21.97007095866891
52-53	21.647967240809432	27.876635817204697	28.639104141417853	21.83629280056802
54-55	21.751674164440338	28.14285611543047	28.453152087106893	21.652317633022296
56-57	21.55377537464236	27.895037402263384	28.538525264698713	22.012661958395547
58-59	21.48982911654953	27.528842329922558	28.870974479566502	22.110354073961407
60-61	21.7316586583851	27.84736718243856	28.541671575889648	21.879302583286687
62-63	21.492873264220954	27.89734134118817	28.60380245591217	22.005982938678702
64-65	21.545435877221788	27.670299423151608	28.66736667438899	22.116898025237614
66-67	21.406598538466177	27.755723279909834	28.60157243877352	22.23610574285047
68-69	21.796976348978575	27.88069612340898	28.419693852350274	21.90263367526217
70-71	21.73676203848189	27.745330231018034	28.422235191039967	22.09567253946011
72-73	21.586038707173905	27.86725558908614	28.472183331022816	22.074522372717137
74-75	21.64825111275993	27.92844183619274	28.323647768504927	22.0996592825424
76-77	21.712123403008416	27.852267710836554	28.25527689093214	22.180331995222893
78-79	21.708059974085575	27.99703642770187	28.31920709445668	21.975696503755877
80-81	21.675367851322285	27.817717181890334	28.43655479910156	22.07036016768582
82-83	21.56426263311892	27.83997483215058	28.511387809200528	22.084374725529972
84-85	21.483303482830667	27.851842557661456	28.323034208599545	22.34181975090833
86-87	21.67222872531693	27.881934402901145	28.264897824906086	22.180939046875835
88-89	21.46325527591441	27.818941879253785	28.27567264848455	22.442130196347257
90-91	21.368705851678165	27.797965405404085	28.37954045655661	22.45378828636114
92-93	21.505007301054864	27.883985526727923	28.246781315848544	22.364225856368673
94-95	21.32731785242129	27.710302110365763	28.362768080105766	22.599611957107182
96-97	21.293073373157714	27.731280296724282	28.37413700239926	22.601509327718748
98-99	21.281551437105225	27.80103926945111	28.26832798805269	22.64908130539098
100	21.353926945110903	27.626940214464085	28.166466728688246	22.852666111736767
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	39.0
1	32.5
2	143.0
3	289.0
4	209.5
5	137.5
6	377.5
7	916.5
8	1545.0
9	1804.5
10	1477.0
11	1192.0
12	1244.5
13	1380.5
14	1546.0
15	1761.5
16	2005.0
17	2228.5
18	2588.0
19	3110.0
20	3888.5
21	4979.0
22	6672.5
23	9489.5
24	12912.5
25	16779.0
26	22141.5
27	29133.5
28	38076.0
29	47689.0
30	56104.5
31	64947.0
32	73769.0
33	82334.0
34	90696.5
35	97415.0
36	102694.0
37	107386.5
38	111243.0
39	113261.0
40	115813.5
41	117579.0
42	116127.0
43	115007.5
44	113299.5
45	110941.0
46	109896.5
47	107182.5
48	104339.5
49	102798.0
50	100349.5
51	96590.5
52	90669.5
53	85875.5
54	83301.0
55	78941.5
56	72457.0
57	65478.5
58	58873.5
59	53514.5
60	47866.5
61	40974.0
62	35087.0
63	29529.5
64	24091.0
65	19528.5
66	15109.0
67	11239.5
68	8455.5
69	6351.5
70	4428.5
71	3064.5
72	2327.0
73	1671.5
74	1219.0
75	851.5
76	508.0
77	334.5
78	212.0
79	91.5
80	36.0
81	14.5
82	12.0
83	9.0
84	6.0
85	4.0
86	2.0
87	0.5
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09196126915732263
2	0.001071096312980463
3	3.06027517994418E-5
4	2.7542476619497625E-4
5	0.039722371835675466
6	1.5301375899720902E-4
7	0.0048352347843118055
8	3.0602751799441803E-4
9	3.6723302159330165E-4
10-11	4.437399010919062E-4
12-13	0.0
14-15	1.224110071977672E-4
16-17	0.0
18-19	1.3771238309748813E-4
20-21	0.0
22-23	1.6831513489692993E-4
24-25	1.0710963129804633E-4
26-27	0.0018208637320667874
28-29	0.009410346178328356
30-31	0.03849826176369779
32-33	0.02797091514468981
34-35	0.031214806835430643
36-37	0.028246339910884787
38-39	0.02742006561229986
40-41	0.03372423248298487
42-43	0.025369681241737257
44-45	0.024099667042060423
46-47	0.017703691915977086
48-49	0.011843264946383979
50-51	0.008721784262840915
52-53	0.006120550359888362
54-55	0.004728125153013759
56-57	0.002968466924545855
58-59	0.0013312197032757187
60-61	6.426577877882779E-4
62-63	0.0016831513489692996
64-65	0.002968466924545855
66-67	5.202467805905107E-4
68-69	0.001254712823777114
70-71	3.978357733927435E-4
72-73	5.202467805905107E-4
74-75	5.049454046907898E-4
76-77	3.825343974930226E-4
78-79	6.12055035988836E-5
80-81	6.579591636879988E-4
82-83	5.814522841893943E-4
84-85	2.2952063849581355E-4
86-87	1.5301375899720902E-4
88-89	0.0
90-91	6.12055035988836E-5
92-93	4.5904127699162706E-5
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3267680.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.658843171644115
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.01749883112628	45.50474696726285
2	14.709800905200082	17.84559012389281
3	4.765505256285186	8.672101079239464
4	2.0245159272201296	4.91219176510966
5	1.0453555784634754	3.1705030046309637
6	0.5912677335772877	2.1519370034110885
7	0.39334405937420475	1.670185692705443
8	0.2694435749814056	1.3075308446723373
9	0.19080547118421595	1.0416635237569516
>10	0.9307980888151294	9.82607672540503
>50	0.04617778051220796	1.880845901733834
>100	0.014982424937255337	1.4572087850635553
>500	3.0262099531829585E-4	0.11307689908682257
>1k	1.5131049586145583E-4	0.2670585070869017
>5k	5.0436831953818604E-5	0.17928317694228937
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5860	0.179332125544729	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4825	0.14765827743230672	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	3.060275179944181E-5	0.0
10-11	0.0	1.5301375899720904E-5	0.0	3.060275179944181E-5	0.0
12-13	0.0	3.060275179944181E-5	0.0	6.120550359888362E-5	0.0
14-15	0.0	3.060275179944181E-5	0.0	6.120550359888362E-5	0.0
16-17	0.0	3.060275179944181E-5	0.0	1.2241100719776723E-4	0.0
18-19	0.0	3.060275179944181E-5	0.0	1.5301375899720902E-4	0.0
20-21	0.0	3.060275179944181E-5	0.0	1.8361651079665082E-4	0.0
22-23	0.0	3.060275179944181E-5	0.0	2.6012339029525533E-4	0.0
24-25	0.0	3.060275179944181E-5	0.0	3.0602751799441803E-4	0.0
26-27	0.0	3.060275179944181E-5	0.0	3.5193164569358073E-4	0.0
28-29	0.0	3.060275179944181E-5	0.0	0.0012700141996768348	0.0
30-31	0.0	3.060275179944181E-5	0.0	0.004881138912010969	0.0
32-33	0.0	3.060275179944181E-5	0.0	0.013266292905058024	0.0
34-35	0.0	3.060275179944181E-5	0.0	0.024849434461146746	0.0
36-37	0.0	3.060275179944181E-5	0.0	0.046256059344856285	0.0
38-39	0.0	3.060275179944181E-5	0.0	0.08238260784409734	0.0
40-41	0.0	3.060275179944181E-5	0.0	0.12669539244968908	0.0
42-43	0.0	3.060275179944181E-5	0.0	0.17221698575135877	0.0
44-45	0.0	3.060275179944181E-5	0.0	0.22474660921510065	0.0
46-47	0.0	6.120550359888362E-5	0.0	0.28915010037702593	0.0
48-49	0.0	6.120550359888362E-5	0.0	0.36181633452480044	0.0
50-51	0.0	6.120550359888362E-5	0.0	0.4390882828183911	0.0
52-53	0.0	6.120550359888362E-5	0.0	0.514202737110121	0.0
54-55	0.0	6.120550359888362E-5	0.0	0.5977329481466973	0.0
56-57	0.0	9.180825539832541E-5	0.0	0.6970235763599864	0.0
58-59	0.0	1.2241100719776723E-4	0.0	0.8119675121186898	0.0
60-61	0.0	1.2241100719776723E-4	0.0	0.9372704793615042	0.0
62-63	0.0	1.2241100719776723E-4	0.0	1.0622215149586252	0.0
64-65	0.0	1.2241100719776723E-4	0.0	1.1930482789012389	0.0
66-67	0.0	1.2241100719776723E-4	0.0	1.3417011457670274	0.0
68-69	0.0	1.8361651079665082E-4	0.0	1.5095113352592664	0.0
70-71	0.0	2.1421926259609263E-4	0.0	1.6932196543113158	0.0
72-73	0.0	2.1421926259609263E-4	0.0	1.8875165254859718	0.0
74-75	0.0	2.1421926259609263E-4	0.0	2.083618959016795	0.0
76-77	0.0	2.4482201439553447E-4	0.0	2.2958490427459237	0.0
78-79	0.0	2.4482201439553447E-4	0.0	2.532729643049503	0.0
80-81	0.0	2.4482201439553447E-4	0.0	2.792623512706262	0.0
82-83	0.0	2.4482201439553447E-4	0.0	3.0676198403760466	0.0
84-85	0.0	2.4482201439553447E-4	0.0	3.340764701561964	0.0
86-87	0.0	2.4482201439553447E-4	0.0	3.625722224942467	0.0
88	0.0	2.4482201439553447E-4	0.0	3.863750428438525	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4375	0.0	49.25123	1
GTATCAA	8345	0.0	41.380848	1
TCAACGC	9475	0.0	35.47404	4
ATCAACG	9520	0.0	35.405117	3
CAACGCA	9675	0.0	34.64408	5
AACGCAG	10040	0.0	33.4675	6
TATCAAC	10525	0.0	32.247715	2
ACGCAGA	11555	0.0	28.876152	7
CGCAGAG	11590	0.0	28.626322	8
GTACATG	9025	0.0	28.149857	1
TACATGG	9210	0.0	26.184336	2
GCAGAGT	13055	0.0	25.017593	9
ACATGGG	9420	0.0	24.353016	3
TCGACCG	200	2.2962922E-8	23.505014	5
GAGTACT	8695	0.0	21.429132	12-13
AGAGTAC	11765	0.0	21.309835	10-11
GTCGTAC	245	8.411007E-9	21.123032	1
CAGAGTA	12985	0.0	20.284824	10-11
GTACTTT	9585	0.0	19.267767	14-15
CATGGGG	6225	0.0	19.256824	4
>>END_MODULE