FastQCFastQC Report
Wed 25 May 2016
SRR1033135_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033135_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2718314
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22000.035.9783171
GTATCAA33300.030.9851881
TCAACGC32100.030.29224
ATCAACG33350.029.1627163
GTACATG77350.028.9935131
CAACGCA34800.027.9419425
TACATGG79750.027.4668312
AACGCAG36250.026.9464136
ACATGGG82300.025.5188523
TATCAAC42650.022.9243812
ACGCAGA42250.022.563947
CATGGGG52750.022.2629684
CGCAGAG43450.021.4003568
ACCGCGT1405.241617E-420.1265268
GAGTACA54400.020.0063251
GTATAGA12250.019.2303921
CTGTCGC7500.018.15869
AGTACAT56550.017.8714242
CGACCCG1355.5038436E-717.39233436-37
TAGACTG11100.016.9278775
Produced by FastQC (version 0.11.5)