##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033135_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2718314
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.617713774052593	33.0	31.0	34.0	30.0	34.0
2	31.704559149531658	34.0	31.0	34.0	30.0	34.0
3	31.857254901383726	34.0	31.0	34.0	30.0	34.0
4	35.222043516679825	37.0	35.0	37.0	33.0	37.0
5	35.30687992630726	37.0	35.0	37.0	33.0	37.0
6	35.44428016778047	37.0	37.0	37.0	35.0	37.0
7	35.345606136745054	37.0	36.0	37.0	33.0	37.0
8	35.35828679100354	37.0	36.0	37.0	33.0	37.0
9	37.02783526847892	39.0	38.0	39.0	34.0	39.0
10-11	37.02214405694118	39.0	38.0	39.0	34.5	39.0
12-13	36.98435372072542	39.0	38.0	39.0	34.0	39.0
14-15	38.44451450421107	41.0	39.0	41.0	34.5	41.0
16-17	38.45296753796655	41.0	39.0	41.0	34.5	41.0
18-19	38.439428631129445	41.0	39.0	41.0	34.0	41.0
20-21	38.481091956264066	41.0	39.0	41.0	34.0	41.0
22-23	38.441319876953145	41.0	39.0	41.0	34.0	41.0
24-25	38.44142324985266	41.0	39.0	41.0	34.0	41.0
26-27	38.40981155966529	41.0	39.0	41.0	34.0	41.0
28-29	38.381495478447306	40.5	39.0	41.0	34.0	41.0
30-31	38.37781801513732	40.5	39.0	41.0	34.0	41.0
32-33	38.45055997945785	41.0	39.0	41.0	34.0	41.0
34-35	38.42508757266452	41.0	39.0	41.0	34.0	41.0
36-37	38.342955412803676	41.0	39.0	41.0	34.0	41.0
38-39	38.26527398968626	41.0	39.0	41.0	33.5	41.0
40-41	38.20666560228141	41.0	39.0	41.0	33.0	41.0
42-43	38.08847726936624	40.5	38.0	41.0	33.0	41.0
44-45	37.958782355533614	40.0	38.0	41.0	33.0	41.0
46-47	37.833506357249384	40.0	38.0	41.0	33.0	41.0
48-49	37.71900155758312	40.0	38.0	41.0	33.0	41.0
50-51	37.605354642620384	40.0	38.0	41.0	32.0	41.0
52-53	37.46537651647308	40.0	37.0	41.0	32.0	41.0
54-55	37.321282052036665	40.0	37.0	41.0	32.0	41.0
56-57	37.17196744011177	40.0	37.0	41.0	31.0	41.0
58-59	36.988511077086756	40.0	36.0	41.0	31.0	41.0
60-61	36.77580367830942	39.5	36.0	41.0	31.0	41.0
62-63	36.524294102888774	39.0	35.0	41.0	31.0	41.0
64-65	36.25930392883236	39.0	35.0	41.0	30.5	41.0
66-67	35.97531502983099	38.5	35.0	40.5	30.0	41.0
68-69	35.66934872130298	37.5	35.0	40.0	30.0	41.0
70-71	35.32973379822934	37.0	35.0	40.0	30.0	41.0
72-73	34.964649227425525	37.0	35.0	39.0	29.0	41.0
74-75	34.59503243554644	36.0	34.0	39.0	29.0	41.0
76-77	34.26371990138004	36.0	34.0	39.0	29.0	40.0
78-79	33.888060577254876	35.0	34.0	37.5	29.0	39.5
80-81	33.5241618517949	35.0	34.0	37.0	28.0	39.0
82-83	33.29861653215927	35.0	34.0	37.0	29.0	39.0
84-85	33.02027727481078	35.0	34.0	36.0	28.5	37.5
86-87	32.75542395028683	35.0	34.0	36.0	28.0	37.0
88-89	32.387881606024905	35.0	34.0	35.5	27.0	37.0
90-91	32.19339892300889	35.0	34.0	35.0	27.0	36.0
92-93	32.01447478105914	35.0	34.0	35.0	27.0	36.0
94-95	31.842073616219466	35.0	33.5	35.0	26.0	36.0
96-97	31.66694318610727	35.0	33.0	35.0	26.0	36.0
98-99	31.478831179915197	35.0	33.0	35.0	25.0	35.5
100	31.31851691894314	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.2281248698410714
1101	2	0.2147346593200865
1101	3	0.2208352426634974
1101	4	0.1460101019079616
1101	5	0.15629038247228522
1101	6	0.18069290974641206
1101	7	0.18471948834208973
1101	8	0.11207041983065125
1101	9	0.3015910877777799
1101	10-11	0.20734749210246406
1101	12-13	0.2270849367549701
1101	14-15	0.31278852404778945
1101	16-17	0.15679587397861638
1101	18-19	0.1807658041037712
1101	20-21	0.20390984763103148
1101	22-23	0.19844578310249972
1101	24-25	0.14320030865455635
1101	26-27	0.19175375445988152
1101	28-29	0.2757115131774981
1101	30-31	0.19275498126030044
1101	32-33	0.15205743581384468
1101	34-35	0.2165395544485449
1101	36-37	0.1696671390602873
1101	38-39	0.1872459792623502
1101	40-41	0.19337060638815728
1101	42-43	0.22701339858357983
1101	44-45	0.18234174216817678
1101	46-47	0.13714646070614123
1101	48-49	0.08913964151206955
1101	50-51	0.08125876163019541
1101	52-53	0.05770062959908984
1101	54-55	0.05733459995029477
1101	56-57	0.07817651574604412
1101	58-59	0.1319246913958736
1101	60-61	0.1696061282753618
1101	62-63	0.17419868015433337
1101	64-65	0.12505781594123988
1101	66-67	0.07242848750062336
1101	68-69	0.08144195514849883
1101	70-71	0.1051935559495547
1101	72-73	0.025046030405647457
1101	74-75	-0.04034058635954807
1101	76-77	-0.001364648585635564
1101	78-79	-8.707815143083053E-5
1101	80-81	0.02025162580819284
1101	82-83	0.04366145130825316
1101	84-85	-0.042125845730311084
1101	86-87	0.07483242432046211
1101	88-89	0.16865422538381125
1101	90-91	0.18933894419635067
1101	92-93	0.18887307592994063
1101	94-95	0.2159736164676751
1101	96-97	0.21503918374240882
1101	98-99	0.29967248730001117
1101	100	0.3212016715953965
1102	1	0.07099183709239654
1102	2	0.04466129687588705
1102	3	0.11575310445726217
1102	4	0.09646749364975449
1102	5	-0.009410079413591177
1102	6	0.0025317715659554096
1102	7	0.058385234581471934
1102	8	0.11693830263190108
1102	9	0.12118636454350451
1102	10-11	0.11694369556636275
1102	12-13	0.08664703354546077
1102	14-15	0.06640654881501717
1102	16-17	0.09950126130012649
1102	18-19	0.09586143865860208
1102	20-21	0.05571910388258772
1102	22-23	0.05850101570722899
1102	24-25	0.057587262973910924
1102	26-27	0.11834041153905872
1102	28-29	0.12584104189225798
1102	30-31	0.07590318398841589
1102	32-33	0.11512172009975785
1102	34-35	0.1574044355838211
1102	36-37	0.1917260742771134
1102	38-39	0.09566548507442008
1102	40-41	0.15630640710763544
1102	42-43	0.1506218010186302
1102	44-45	0.13139522101401724
1102	46-47	0.13555355015620307
1102	48-49	0.09442718620087476
1102	50-51	0.06613619708706153
1102	52-53	0.1326789079332329
1102	54-55	0.11690349020484092
1102	56-57	0.0950327374791371
1102	58-59	0.09904258342925942
1102	60-61	0.1611076440888013
1102	62-63	0.09631046503772467
1102	64-65	0.11055840366297787
1102	66-67	-3.570348261874301E-4
1102	68-69	0.009768784175470557
1102	70-71	0.05993692674796591
1102	72-73	0.03794364837328601
1102	74-75	0.05547815844519732
1102	76-77	0.07985009943259058
1102	78-79	0.08465480156872474
1102	80-81	0.07739984804573652
1102	82-83	0.016779644344353528
1102	84-85	0.03975633400315104
1102	86-87	0.10640043246984021
1102	88-89	0.23641840229815614
1102	90-91	0.19816476883578815
1102	92-93	0.1486843360791994
1102	94-95	0.17393022609014608
1102	96-97	0.16917351177823292
1102	98-99	0.1901647730602818
1102	100	0.2176273340278172
1103	1	-0.05749502907764992
1103	2	-0.04875680582187414
1103	3	-0.006237506619129363
1103	4	-0.03775598324844509
1103	5	-0.038247275532228286
1103	6	0.033618611010773236
1103	7	-0.05353300241731063
1103	8	-0.07954463034041481
1103	9	-0.03612615730572344
1103	10-11	0.09551161476439063
1103	12-13	-0.0026894316786751915
1103	14-15	-0.005943199387445475
1103	16-17	-0.043343699982038686
1103	18-19	-0.03544311691300095
1103	20-21	0.04874294197630036
1103	22-23	-0.0010931725004681425
1103	24-25	-0.007409740561115541
1103	26-27	0.04452772704387087
1103	28-29	0.0896520459374841
1103	30-31	0.05975490351389112
1103	32-33	-0.01421488684906791
1103	34-35	0.006229436629887175
1103	36-37	0.04549244179219869
1103	38-39	0.008829887070440634
1103	40-41	0.03100983131123769
1103	42-43	-0.021269062867617095
1103	44-45	-0.05280068705658891
1103	46-47	-0.03988066731426443
1103	48-49	0.025086253274444914
1103	50-51	0.024703589879081278
1103	52-53	-0.113914705277125
1103	54-55	-0.026457888870922375
1103	56-57	-0.08871818879298132
1103	58-59	-0.10821054902393712
1103	60-61	-0.07058803789718127
1103	62-63	-0.02697260776962196
1103	64-65	0.0010198592700803033
1103	66-67	-0.01476554343697245
1103	68-69	-0.06864084203576937
1103	70-71	-0.06532600630534091
1103	72-73	-0.005818676613593254
1103	74-75	-0.0056582981404744714
1103	76-77	-0.0758002616954343
1103	78-79	-0.13131041784134823
1103	80-81	-0.06051758179715705
1103	82-83	-0.06577688018818151
1103	84-85	-0.07417351632206959
1103	86-87	0.016145591221743416
1103	88-89	-0.05942477865188778
1103	90-91	-0.012114995712252608
1103	92-93	0.04038996578901788
1103	94-95	0.08012963543560758
1103	96-97	0.061862339688008916
1103	98-99	0.18459624107167016
1103	100	0.2084624029262656
1104	1	0.12988480257246238
1104	2	0.073910163298752
1104	3	0.17998009204868737
1104	4	0.08933956590274761
1104	5	0.0194820669314808
1104	6	0.029221435667764695
1104	7	0.06130848955969981
1104	8	0.029673605764692468
1104	9	0.13575844578055296
1104	10-11	0.0776307978895403
1104	12-13	0.11574257639414753
1104	14-15	0.05516084927373299
1104	16-17	0.0606052967290438
1104	18-19	0.032853773140487874
1104	20-21	0.020624586273825685
1104	22-23	0.06419159274819464
1104	24-25	0.07213443986478296
1104	26-27	0.12530798039924207
1104	28-29	0.17061268158911957
1104	30-31	0.10397322267272102
1104	32-33	0.08840239538407246
1104	34-35	0.029770883550256144
1104	36-37	0.08666775054440023
1104	38-39	0.05788232199785881
1104	40-41	0.05256071417227304
1104	42-43	0.08024542170100091
1104	44-45	0.014078839371101992
1104	46-47	-0.028560575166167723
1104	48-49	-0.061963387933488434
1104	50-51	-0.07517512573667773
1104	52-53	-0.06636177929459564
1104	54-55	-0.06331648057053485
1104	56-57	-0.06795177426356247
1104	58-59	0.004629348321664395
1104	60-61	-0.04436447493415585
1104	62-63	-0.09695198449298204
1104	64-65	-0.15114208094785653
1104	66-67	-0.11114107913346061
1104	68-69	-0.08406002453801165
1104	70-71	0.012578901338926585
1104	72-73	0.013891268654006694
1104	74-75	-0.06918933220080703
1104	76-77	-0.08063865992140506
1104	78-79	-0.05792337297572914
1104	80-81	-0.06332635476151438
1104	82-83	-0.0970070251584545
1104	84-85	-0.10338532614566986
1104	86-87	0.013143069503193772
1104	88-89	0.09340691194809381
1104	90-91	0.022388374192402694
1104	92-93	-0.014489336393637586
1104	94-95	0.06285251528179003
1104	96-97	0.03535704293707198
1104	98-99	0.02957530634358463
1104	100	0.056189897444721026
1105	1	0.10646981078669526
1105	2	0.08543828895629346
1105	3	0.0887645422615293
1105	4	0.08320949169139169
1105	5	0.041875843334374
1105	6	0.010562631870598693
1105	7	-0.037606766255009916
1105	8	0.0018729263706873667
1105	9	0.025906094607904606
1105	10-11	0.06817184730847003
1105	12-13	0.05946470667269921
1105	14-15	0.08540929696565058
1105	16-17	0.18612880238593021
1105	18-19	0.15766194751692808
1105	20-21	0.10729158002615691
1105	22-23	0.18672220139053763
1105	24-25	0.16794027439856052
1105	26-27	0.1982642471918865
1105	28-29	0.15955349813655317
1105	30-31	0.21711442858697438
1105	32-33	0.21471406789108016
1105	34-35	0.1363816092960093
1105	36-37	0.1314185694387504
1105	38-39	0.14311024422161012
1105	40-41	0.16443640206377097
1105	42-43	0.14774679748398256
1105	44-45	0.1321667943910967
1105	46-47	0.1664057662020184
1105	48-49	0.15893829618673294
1105	50-51	0.12607770928670448
1105	52-53	0.14740581251868434
1105	54-55	0.1366248083964905
1105	56-57	0.16870635529878086
1105	58-59	0.14619286498481898
1105	60-61	0.15555949029494798
1105	62-63	0.2315567299794381
1105	64-65	0.17990961915573678
1105	66-67	0.20192845455704145
1105	68-69	0.1113531301728301
1105	70-71	0.0590674681777017
1105	72-73	0.04498510211142559
1105	74-75	0.08974616330361584
1105	76-77	0.11156302784060301
1105	78-79	0.1425301043157532
1105	80-81	0.1316466252653541
1105	82-83	0.13139248653975244
1105	84-85	0.11171796379655063
1105	86-87	0.14463928154776795
1105	88-89	0.15696498769303702
1105	90-91	0.09663096013836991
1105	92-93	0.08966173469388394
1105	94-95	0.1043134678294173
1105	96-97	0.1321809315876692
1105	98-99	0.21982503877402948
1105	100	0.28043405137865207
1106	1	0.12488901099714056
1106	2	0.013319124307209051
1106	3	0.018586516257897756
1106	4	0.08572521419588952
1106	5	0.059904964676547934
1106	6	0.10453364205673665
1106	7	0.03789986131040024
1106	8	0.06473761296795288
1106	9	0.055115731248768896
1106	10-11	0.12802301328258636
1106	12-13	0.10035442262027061
1106	14-15	0.15202826432227567
1106	16-17	0.11063465783144721
1106	18-19	0.16212532418965253
1106	20-21	0.15936656090583767
1106	22-23	0.16254467957875818
1106	24-25	0.2112130884711121
1106	26-27	0.20288564051512026
1106	28-29	0.24375926199939357
1106	30-31	0.15398790322393552
1106	32-33	0.1432797638463441
1106	34-35	0.07977700044659031
1106	36-37	0.038154645705688495
1106	38-39	0.04372660049769905
1106	40-41	0.04587714855999536
1106	42-43	0.16414770795217493
1106	44-45	0.13395728440711707
1106	46-47	0.15000802215610065
1106	48-49	0.11619390927916129
1106	50-51	0.11715844574781897
1106	52-53	0.03486257867595555
1106	54-55	0.047778835354925775
1106	56-57	0.07022964798824205
1106	58-59	0.05494449082087982
1106	60-61	0.0051141208221849865
1106	62-63	0.1176412174330963
1106	64-65	0.09260057877654759
1106	66-67	0.12083120398586544
1106	68-69	0.10286498720643067
1106	70-71	0.09453846131387422
1106	72-73	0.15520576520897578
1106	74-75	0.13831561025143202
1106	76-77	0.03706119131567931
1106	78-79	0.07421186426197579
1106	80-81	0.05186605832962954
1106	82-83	0.010178370629205347
1106	84-85	-0.06604632285722545
1106	86-87	-0.03564505099477344
1106	88-89	0.07857490638906484
1106	90-91	0.12120854722716956
1106	92-93	0.12980971622145887
1106	94-95	0.11677687129494174
1106	96-97	0.05142882104543389
1106	98-99	-0.005646027433424905
1106	100	-0.007034832433138405
1107	1	0.15201456928442525
1107	2	0.07680539311236956
1107	3	0.0794949036964887
1107	4	0.11461428640491533
1107	5	0.0056122525041431
1107	6	0.091285444145349
1107	7	0.026390080535470872
1107	8	0.06689454223684521
1107	9	0.09016234620366248
1107	10-11	0.048939382893166794
1107	12-13	0.07008072823404632
1107	14-15	0.054758817799346104
1107	16-17	0.012040135901976612
1107	18-19	0.10505645442802347
1107	20-21	0.05628058582601625
1107	22-23	0.09588500360614205
1107	24-25	0.002633241189698765
1107	26-27	0.09918932555976312
1107	28-29	0.13240726564307437
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2216	8	0.10148328698261366
2216	9	-0.023767763896863414
2216	10-11	0.05539276150755512
2216	12-13	0.009517081259609483
2216	14-15	5.989444508784914E-4
2216	16-17	-0.06929464414549358
2216	18-19	-0.11200110314194944
2216	20-21	-0.10384929199376813
2216	22-23	-0.0824861038605107
2216	24-25	-0.11545422769812319
2216	26-27	-0.18718358121510903
2216	28-29	-0.09049664665138835
2216	30-31	-0.19288464250138304
2216	32-33	-0.1119904772715472
2216	34-35	-0.14720851398926982
2216	36-37	-0.1623624544376696
2216	38-39	-0.15241729281951422
2216	40-41	-0.13696996913981252
2216	42-43	-0.15094962298992698
2216	44-45	-0.022371786731305576
2216	46-47	0.010415812011537184
2216	48-49	-0.06818209107077422
2216	50-51	0.01901591539185432
2216	52-53	0.024179363280360633
2216	54-55	0.052292886481382084
2216	56-57	-0.02083634478637464
2216	58-59	-0.1435714123820233
2216	60-61	-0.01622049759836841
2216	62-63	-0.04770290791558551
2216	64-65	0.007878444922020833
2216	66-67	-0.08733316755081688
2216	68-69	-0.03971793372591037
2216	70-71	-0.1509460709150403
2216	72-73	-0.06436642302482909
2216	74-75	-0.08445829425976115
2216	76-77	-0.14617699486224467
2216	78-79	-0.09788152890512691
2216	80-81	-0.0582747810000086
2216	82-83	-0.10120054154677405
2216	84-85	-0.15299544692348377
2216	86-87	-0.1617190415981895
2216	88-89	-0.04187674477238801
2216	90-91	-0.11223040483358204
2216	92-93	-0.09271714534899544
2216	94-95	-0.09028681707107467
2216	96-97	-0.10070322912267571
2216	98-99	-0.08946670308841576
2216	100	-0.0640454339056511
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	28131.0
3	4698.0
4	2977.0
5	2443.0
6	2136.0
7	2356.0
8	2827.0
9	3037.0
10	3362.0
11	3689.0
12	4173.0
13	4667.0
14	5318.0
15	5549.0
16	5571.0
17	5587.0
18	5590.0
19	5352.0
20	5327.0
21	5745.0
22	6559.0
23	8805.0
24	13508.0
25	18652.0
26	19622.0
27	16625.0
28	17782.0
29	22835.0
30	27982.0
31	33855.0
32	44716.0
33	61290.0
34	86481.0
35	133166.0
36	248637.0
37	532049.0
38	900397.0
39	415870.0
40	948.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.486869156636295	20.334370513090242	13.413354888955329	23.76540544131814
2	15.007255985267035	20.44206261510129	41.050313075506445	23.500368324125233
3	17.8602823919532	25.999113567859915	30.266319848864793	25.874284191322094
4	11.81478094298758	16.96479667600834	39.871302050077006	31.34912033092707
5	12.39725782879427	38.57989920934578	36.200649438356905	12.822193523503037
6	30.225625154522657	39.04371813889968	18.11822307773814	12.61243362883952
7	26.612894324987142	32.939238284023624	23.22798869515247	17.219878695836762
8	24.677836663629982	37.229365872468065	20.67456351945416	17.4182339444478
9	24.70899299581574	17.057852589811482	21.48440432054703	36.74875009382575
10-11	23.40249500085458	27.393826687829055	29.477434071901225	19.72624423941514
12-13	23.992535309003916	24.694741675625913	29.46838360446557	21.844339410904603
14-15	21.332230233615768	26.744244233194834	26.293934496923242	25.629591036266152
16-17	21.200376411197038	30.115029863610733	26.90760540111433	21.776988324077898
18-19	21.12617391805078	29.239447730739393	28.6694764473133	20.964901903896525
20-21	22.194653468989753	28.472853384357812	28.205655621584825	21.126837525067614
22-23	21.812686209526017	28.228126963277344	28.27318058518198	21.686006242014663
24-25	21.73754976781154	28.416371678159567	28.12668805793305	21.719390496095848
26-27	21.44056658341195	28.40592487059215	28.223532286099896	21.929976259896
28-29	21.55196155971055	28.34726661364532	28.344507001779213	21.756264824864914
30-31	21.725353822876624	28.74512831230609	27.98440544804809	21.545112416769204
32-33	21.363002867417144	28.469634540003554	28.470646461357024	21.69671613122228
34-35	21.524482597554453	28.280540031300138	28.501171593516194	21.693805777629212
36-37	21.869716452815318	28.569560109026753	27.899833281120628	21.6608901570373
38-39	21.648767080951014	28.69388213132004	28.107850062915297	21.549500724813644
40-41	21.477600837071307	28.37019434535372	28.413295129445835	21.738909688129127
42-43	21.43138197392681	28.312012213034976	28.36556105147035	21.891044761567866
44-45	21.545894999490443	28.444226642281656	28.34066024223868	21.669218115989217
46-47	21.645452938377144	28.521401329352354	28.20627201338587	21.62687371888464
48-49	21.580789149262028	28.426053631953284	28.243671439077357	21.749485779707328
50-51	21.595472494717438	28.35459449038985	28.214774690721583	21.83515832417113
52-53	21.583642845041556	28.373818648182358	28.24157677781986	21.80096172895622
54-55	21.68398739325303	28.691018079064033	28.07085280221262	21.554141725470316
56-57	21.555688729815547	28.4138872058164	28.133890163609358	21.896533900758687
58-59	21.405841359675406	28.092988212580433	28.49346837674566	22.007702050998496
60-61	21.646245789976142	28.50984929448186	28.14394740666209	21.69995750887991
62-63	21.510060756336372	28.505611177841505	28.13542611290047	21.848901952921658
64-65	21.51516623053936	28.263459153578246	28.26772661605309	21.9536479998293
66-67	21.4058113875148	28.287329443231556	28.149171540023758	22.157687629229876
68-69	21.719804342691656	28.49689981171516	28.034814669294857	21.74848117629833
70-71	21.662871332658877	28.265409730525153	28.13219902510807	21.9395199117079
72-73	21.54427548454621	28.372951128753083	28.220335397762913	21.862437988937796
74-75	21.546094563080867	28.461005595881762	28.133221055171916	21.859678785865462
76-77	21.616880821158343	28.4147392639786	28.029412013572173	21.938967901290887
78-79	21.723419283872225	28.444447305783545	28.116145432873594	21.715987977470633
80-81	21.686661442640563	28.327318345528855	28.187851564080773	21.798168647749804
82-83	21.510768407276366	28.355459987620723	28.337672824728386	21.796098780374525
84-85	21.57594985838164	28.317178772439767	28.106507896157083	22.00036347302151
86-87	21.67401268388189	28.44724166587878	28.05757962926924	21.821166020970086
88-89	21.535319775780355	28.237277533805976	28.17187705301806	22.055525637395604
90-91	21.532537891098396	28.254881072329376	28.19667391927177	22.01590711730046
92-93	21.714575378699337	28.384873161701087	28.06886240012348	21.831689059476094
94-95	21.502816133334296	28.17178848198968	28.21619969204527	22.109195692630756
96-97	21.561991651214004	28.201717239323415	28.164893612099913	22.071397497362668
98-99	21.53198405857869	28.28871380974286	28.086189368956415	22.09311276272203
100	21.7204878882822	28.147466372376385	27.99465895709032	22.1373867822511
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	350.0
1	351.5
2	340.0
3	297.0
4	251.0
5	207.0
6	187.5
7	199.0
8	217.5
9	219.5
10	186.5
11	188.5
12	220.0
13	237.0
14	285.5
15	361.0
16	457.5
17	620.5
18	894.5
19	1273.5
20	1846.0
21	2767.0
22	4275.0
23	6696.0
24	9921.5
25	13887.0
26	19026.5
27	25760.5
28	34317.0
29	42980.0
30	50392.0
31	57836.0
32	66087.5
33	74827.5
34	82135.5
35	87495.0
36	91467.5
37	95644.5
38	99248.5
39	99939.5
40	100059.5
41	99633.0
42	97816.5
43	96203.5
44	93946.0
45	91070.5
46	89063.0
47	85972.0
48	82847.5
49	81166.5
50	79013.5
51	76146.0
52	71523.0
53	67516.5
54	65171.5
55	62373.5
56	57560.5
57	52156.0
58	47652.5
59	42506.5
60	37217.5
61	31891.5
62	27341.0
63	23298.5
64	19347.0
65	15903.5
66	12478.0
67	9394.0
68	7306.0
69	5775.0
70	4307.0
71	3039.0
72	2169.0
73	1673.5
74	1270.5
75	879.5
76	572.5
77	371.5
78	276.5
79	199.5
80	137.0
81	91.0
82	67.0
83	37.5
84	23.0
85	25.5
86	24.5
87	20.0
88	14.0
89	9.0
90	7.0
91	6.5
92	5.5
93	4.5
94	4.5
95	3.0
96	1.0
97	0.0
98	0.0
99	0.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3327062289345528
2	0.12191380392404998
3	0.06643824076247262
4	0.04042947209189225
5	0.04403464794722023
6	0.009785477321604494
7	0.006916051640833251
8	0.016112928822792364
9	0.01876162945119659
10-11	0.02205411148233795
12-13	0.016627953944982073
14-15	0.01905592952101928
16-17	0.019920435976123436
18-19	0.020067586011034783
20-21	0.0244269057952834
22-23	0.01624168510333979
24-25	0.025530531057118493
26-27	0.020233129800310046
28-29	0.019883648467395598
30-31	0.020968879974866773
32-33	0.026174312459855627
34-35	0.015984172542244934
36-37	0.009767083567240577
38-39	0.013758528264210831
40-41	0.009785477321604494
42-43	0.008626670796677647
44-45	0.009233664690686948
46-47	0.008222008200671446
48-49	0.00445128855606821
50-51	0.006345845255551787
52-53	0.006934445395197169
54-55	0.0032005132593217705
56-57	0.0026119131196763877
58-59	0.002832638172043406
60-61	0.003641963364055808
62-63	0.002906213189499079
64-65	0.0026119131196763877
66-67	0.0017474066645722312
68-69	0.0033844508029609527
70-71	0.0023544005585815326
72-73	0.0018025879276639858
74-75	0.002391188067309369
76-77	0.0038626884164228267
78-79	0.0030901507331382613
80-81	0.0038259009076949905
82-83	0.0022624317867619417
84-85	0.0034212383116887894
86-87	0.002244038032398023
88-89	0.6069571065005736
90-91	0.6164114962436275
92-93	0.6130822267037583
94-95	0.5993972734570031
96-97	0.5975946855293391
98-99	0.5948908036378432
100	0.5966933915655072
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2718314.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.85466387413904
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.15265117173718	50.037092587417135
2	13.807649643972516	17.909809531034263
3	4.259061509622624	8.286600078775754
4	1.829333458023114	4.745632261352219
5	0.9146545604465203	2.9659807039353723
6	0.5294788661647658	2.0603504336145684
7	0.33428045019035313	1.5175752365753858
8	0.22424353822644633	1.1634591438099058
9	0.16787941046388846	0.9798986463321696
>10	0.7417199853633276	8.259974262904946
>50	0.0316667874497157	1.364978426257799
>100	0.007323799795751457	0.6896343585638249
>500	5.6818543851443715E-5	0.0190143294266456
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	7.357501745567289E-5	0.0
5	0.0	0.0	0.0	7.357501745567289E-5	0.0
6	0.0	0.0	0.0	7.357501745567289E-5	0.0
7	0.0	0.0	0.0	7.357501745567289E-5	0.0
8	0.0	0.0	0.0	7.357501745567289E-5	0.0
9	0.0	0.0	0.0	7.357501745567289E-5	0.0
10-11	0.0	0.0	0.0	1.1036252618350933E-4	0.0
12-13	0.0	0.0	0.0	1.4715003491134579E-4	0.0
14-15	0.0	0.0	0.0	1.4715003491134579E-4	0.0
16-17	0.0	0.0	0.0	1.4715003491134579E-4	0.0
18-19	0.0	0.0	0.0	1.4715003491134579E-4	0.0
20-21	0.0	0.0	0.0	1.4715003491134579E-4	0.0
22-23	0.0	0.0	0.0	1.4715003491134579E-4	0.0
24-25	0.0	0.0	0.0	2.0233129800310045E-4	0.0
26-27	0.0	0.0	0.0	4.4145010473403733E-4	0.0
28-29	0.0	0.0	0.0	0.0010300502443794206	0.0
30-31	0.0	0.0	0.0	0.004065019714425928	0.0
32-33	0.0	0.0	0.0	0.012415784195644802	0.0
34-35	0.0	0.0	0.0	0.024445299549647317	0.0
36-37	0.0	0.0	0.0	0.04585562962924813	0.0
38-39	0.0	0.0	0.0	0.0828638634094516	0.0
40-41	0.0	0.0	0.0	0.1257580985861089	0.0
42-43	0.0	0.0	0.0	0.1703629529186106	0.0
44-45	0.0	0.0	3.6787508727836446E-5	0.22181028387448987	0.0
46-47	0.0	0.0	3.6787508727836446E-5	0.2847169237990902	0.0
48-49	0.0	0.0	3.6787508727836446E-5	0.35575360315254234	0.0
50-51	0.0	0.0	3.6787508727836446E-5	0.43379830291864735	0.0
52-53	0.0	0.0	3.6787508727836446E-5	0.510794558686009	0.0
54-55	0.0	0.0	3.6787508727836446E-5	0.593805572130372	0.0
56-57	0.0	0.0	3.6787508727836446E-5	0.6949896148862862	0.0
58-59	0.0	0.0	3.6787508727836446E-5	0.8082399605049306	0.0
60-61	0.0	0.0	3.6787508727836446E-5	0.9302641269551641	0.0
62-63	0.0	0.0	3.6787508727836446E-5	1.0556543504539946	0.0
64-65	0.0	0.0	3.6787508727836446E-5	1.187574356752016	0.0
66-67	0.0	0.0	3.6787508727836446E-5	1.3363246482930227	0.0
68-69	0.0	0.0	3.6787508727836446E-5	1.5021995251468374	0.0
70-71	0.0	0.0	3.6787508727836446E-5	1.6874614191002215	0.0
72-73	0.0	0.0	3.6787508727836446E-5	1.8816258901657426	0.0
74-75	0.0	0.0	3.6787508727836446E-5	2.0743740421452417	0.0
76-77	0.0	0.0	3.6787508727836446E-5	2.2829224291233463	0.0
78-79	0.0	0.0	3.6787508727836446E-5	2.5212686981709984	0.0
80-81	0.0	0.0	3.6787508727836446E-5	2.780786178491521	0.0
82-83	0.0	0.0	3.6787508727836446E-5	3.051155973886755	0.0
84-85	0.0	0.0	3.6787508727836446E-5	3.3168905431822813	0.0
86-87	0.0	0.0	3.6787508727836446E-5	3.598664466283145	0.0
88	0.0	0.0	3.6787508727836446E-5	3.8322283591961783	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2200	0.0	35.978317	1
GTATCAA	3330	0.0	30.985188	1
TCAACGC	3210	0.0	30.2922	4
ATCAACG	3335	0.0	29.162716	3
GTACATG	7735	0.0	28.993513	1
CAACGCA	3480	0.0	27.941942	5
TACATGG	7975	0.0	27.466831	2
AACGCAG	3625	0.0	26.946413	6
ACATGGG	8230	0.0	25.518852	3
TATCAAC	4265	0.0	22.924381	2
ACGCAGA	4225	0.0	22.56394	7
CATGGGG	5275	0.0	22.262968	4
CGCAGAG	4345	0.0	21.400356	8
ACCGCGT	140	5.241617E-4	20.126526	8
GAGTACA	5440	0.0	20.006325	1
GTATAGA	1225	0.0	19.230392	1
CTGTCGC	750	0.0	18.1586	9
AGTACAT	5655	0.0	17.871424	2
CGACCCG	135	5.5038436E-7	17.392334	36-37
TAGACTG	1110	0.0	16.927877	5
>>END_MODULE