FastQCFastQC Report
Wed 25 May 2016
SRR1033135_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033135_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2718314
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50500.18577691907557403No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41180.15149096094123046No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40550.048.6361961
GTATCAA72050.040.7649651
TCAACGC83750.034.416314
ATCAACG84600.034.070523
CAACGCA85950.033.4265825
AACGCAG88950.032.3354076
TATCAAC89500.032.2577442
ACGCAGA103300.027.7070227
CGCAGAG103150.027.5630368
GTACATG74300.026.9238151
TATACCG1755.5333658E-926.8694365
TACATGG75250.024.6819362
GCAGAGT115650.024.2588169
ACATGGG75700.023.6034983
GTGGTAT20600.021.2496721
AGAGTAC109350.020.47788210-11
GAGTACT79750.020.24086212-13
CAGAGTA114600.019.90882110-11
CATGGGG50750.019.8274254
TGGTATC21000.019.4799842
Produced by FastQC (version 0.11.5)