##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033135_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2718314
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.755922972842725	34.0	31.0	34.0	31.0	34.0
2	32.85807894157923	34.0	31.0	34.0	31.0	34.0
3	32.95743758815207	34.0	31.0	34.0	31.0	34.0
4	36.37800121693079	37.0	37.0	37.0	35.0	37.0
5	36.31540579933002	37.0	37.0	37.0	35.0	37.0
6	36.40134473059404	37.0	37.0	37.0	35.0	37.0
7	36.371455983377935	37.0	37.0	37.0	35.0	37.0
8	36.37180509683576	37.0	37.0	37.0	35.0	37.0
9	38.193516275161734	39.0	39.0	39.0	37.0	39.0
10-11	38.175460965878116	39.0	39.0	39.0	37.0	39.0
12-13	38.13970442708238	39.0	39.0	39.0	37.0	39.0
14-15	39.6744665995172	41.0	40.0	41.0	37.0	41.0
16-17	39.68746528178863	41.0	40.0	41.0	37.0	41.0
18-19	39.70456301222008	41.0	40.0	41.0	37.0	41.0
20-21	39.69846327539791	41.0	40.0	41.0	37.0	41.0
22-23	39.63081638103618	41.0	40.0	41.0	37.0	41.0
24-25	39.584309796439996	41.0	40.0	41.0	37.0	41.0
26-27	39.48626685511681	41.0	39.5	41.0	36.5	41.0
28-29	39.39745095673274	41.0	39.0	41.0	36.0	41.0
30-31	39.30461363183208	41.0	39.0	41.0	36.0	41.0
32-33	39.33908683838585	41.0	39.0	41.0	36.0	41.0
34-35	39.28327779645766	41.0	39.0	41.0	35.0	41.0
36-37	39.19658766426542	41.0	39.0	41.0	35.0	41.0
38-39	39.11035443293159	41.0	39.0	41.0	35.0	41.0
40-41	38.99982562720863	41.0	39.0	41.0	35.0	41.0
42-43	38.89093736043739	41.0	39.0	41.0	35.0	41.0
44-45	38.75497734993088	41.0	39.0	41.0	35.0	41.0
46-47	38.549048417511734	41.0	39.0	41.0	35.0	41.0
48-49	38.37403276442677	41.0	39.0	41.0	35.0	41.0
50-51	38.25162821513629	41.0	38.0	41.0	34.5	41.0
52-53	38.124702481023164	40.0	38.0	41.0	34.0	41.0
54-55	37.99234617487163	40.0	38.0	41.0	34.0	41.0
56-57	37.84160935785932	40.0	37.5	41.0	34.0	41.0
58-59	37.66218950422946	40.0	37.0	41.0	34.0	41.0
60-61	37.47645047628788	40.0	36.5	41.0	33.0	41.0
62-63	37.25053856912777	39.5	36.0	41.0	33.0	41.0
64-65	37.00722672215204	39.0	35.5	41.0	33.0	41.0
66-67	36.73268963776812	39.0	35.0	41.0	33.0	41.0
68-69	36.41633821552624	38.5	35.0	41.0	32.0	41.0
70-71	36.08478895374118	37.0	35.0	40.0	32.0	41.0
72-73	35.73382545210009	37.0	35.0	39.5	31.5	41.0
74-75	35.39810908526388	36.5	35.0	39.0	31.0	41.0
76-77	35.05497782816849	36.0	35.0	39.0	31.0	41.0
78-79	34.71089874091072	35.5	35.0	38.0	31.0	39.5
80-81	34.364955998460815	35.0	35.0	37.0	31.0	39.0
82-83	34.03258527160585	35.0	34.5	37.0	31.0	39.0
84-85	33.75130448505949	35.0	34.0	36.0	30.0	38.0
86-87	33.51034317595392	35.0	34.0	36.0	30.0	37.0
88-89	33.287984758199386	35.0	34.0	36.0	30.0	37.0
90-91	33.081711126823464	35.0	34.0	35.0	30.0	36.5
92-93	32.91041689812141	35.0	34.0	35.0	30.0	36.0
94-95	32.75990374916216	35.0	34.0	35.0	29.5	36.0
96-97	32.63595541206792	35.0	34.0	35.0	29.5	36.0
98-99	32.52627161542044	35.0	34.0	35.0	29.0	36.0
100	32.428721994589296	35.0	34.0	35.0	29.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.11527548698319379
1101	2	0.13937704941717044
1101	3	0.12864130734644874
1101	4	0.10517780420646972
1101	5	0.09196499854521534
1101	6	0.11396902204033665
1101	7	0.1083339146083162
1101	8	0.11911201106980229
1101	9	0.1306622651026217
1101	10-11	0.13053419620056417
1101	12-13	0.1680219327247059
1101	14-15	0.20459153984914025
1101	16-17	0.20675726212963497
1101	18-19	0.23505835960586552
1101	20-21	0.2412564346022279
1101	22-23	0.21407520462375373
1101	24-25	0.2231888707828631
1101	26-27	0.18807384288867013
1101	28-29	0.19627429368004812
1101	30-31	0.19654026894139065
1101	32-33	0.16162360445625978
1101	34-35	0.16497187084828369
1101	36-37	0.23192427131265703
1101	38-39	0.2504192099982987
1101	40-41	0.2592902668168193
1101	42-43	0.15486932885895754
1101	44-45	0.1355558672343733
1101	46-47	0.21302339503446888
1101	48-49	0.14774054223469335
1101	50-51	0.10511825838602817
1101	52-53	0.20164363251921458
1101	54-55	0.19502024267060136
1101	56-57	0.1411661500508714
1101	58-59	0.12243382936662073
1101	60-61	0.16145940078547483
1101	62-63	0.15879813597936732
1101	64-65	0.14964676248331443
1101	66-67	0.19474881867787275
1101	68-69	0.1873023231929949
1101	70-71	0.2149127500530028
1101	72-73	0.29875994630773306
1101	74-75	0.2610083943577095
1101	76-77	0.23157778375266247
1101	78-79	0.19951139173021204
1101	80-81	0.12577584628667893
1101	82-83	0.15949244872911805
1101	84-85	0.1967914896862908
1101	86-87	0.08411400004049341
1101	88-89	0.09612633201378884
1101	90-91	0.08438717537173801
1101	92-93	0.06957673187383051
1101	94-95	0.09642725061148383
1101	96-97	0.07807158431172212
1101	98-99	0.04204792925398948
1101	100	0.05919970619329007
1102	1	0.11637979069493554
1102	2	0.1363755228038599
1102	3	0.08880192573980139
1102	4	0.059548091893994126
1102	5	0.03483168709032469
1102	6	0.06163475295087295
1102	7	0.05883144004548768
1102	8	0.07524330399491674
1102	9	0.12154209185883502
1102	10-11	0.07115291006891766
1102	12-13	0.09176320834912843
1102	14-15	0.09935499839821205
1102	16-17	0.046709241048297656
1102	18-19	0.1003918002410984
1102	20-21	0.12303350725327533
1102	22-23	0.11936619830199646
1102	24-25	0.07546833926222973
1102	26-27	0.12721255706359358
1102	28-29	0.10599274465876363
1102	30-31	0.1474364139466644
1102	32-33	0.142471958312818
1102	34-35	0.1200216094566926
1102	36-37	0.1523867424752794
1102	38-39	0.08682712581229168
1102	40-41	0.04680705015687181
1102	42-43	0.04585356593204182
1102	44-45	0.08608328391341047
1102	46-47	0.12279849305195967
1102	48-49	0.07556608329056047
1102	50-51	0.10710128796441865
1102	52-53	0.11894620226022568
1102	54-55	0.03993486450458761
1102	56-57	0.03731181724917576
1102	58-59	0.06403648069886714
1102	60-61	0.07831227804395269
1102	62-63	0.012386025636786258
1102	64-65	-1.0553736555607429E-4
1102	66-67	-0.018846261599968273
1102	68-69	0.019843956676204755
1102	70-71	-0.03159492168403233
1102	72-73	-0.00667461591098828
1102	74-75	-0.011055766600712502
1102	76-77	-0.01560359058949956
1102	78-79	0.006727513770087512
1102	80-81	-0.03914946393076235
1102	82-83	-0.07861763047222325
1102	84-85	0.034376834755946106
1102	86-87	-0.01542672695073577
1102	88-89	0.010589686675110954
1102	90-91	-0.009844894934687431
1102	92-93	-0.0055030303618934795
1102	94-95	0.007604937670379286
1102	96-97	0.021858282082007463
1102	98-99	0.06869746942356159
1102	100	0.012990354624207612
1103	1	0.08136751463682401
1103	2	0.1120758790052605
1103	3	0.09118506023114037
1103	4	0.03230779564889019
1103	5	0.027197315207423856
1103	6	0.06822771827691554
1103	7	0.06443374975308558
1103	8	0.09241898727666609
1103	9	0.10235162331699144
1103	10-11	0.06408921629143549
1103	12-13	0.0716099567122015
1103	14-15	0.13178319459378685
1103	16-17	0.13629328581711064
1103	18-19	0.056678471505939854
1103	20-21	0.09532636311386256
1103	22-23	0.09633326958142163
1103	24-25	0.08396230516461856
1103	26-27	0.09205258362685953
1103	28-29	0.10947289577778463
1103	30-31	0.14275207533532352
1103	32-33	0.1518920950974021
1103	34-35	0.1667197277576662
1103	36-37	0.1277030247530746
1103	38-39	0.08947025763110616
1103	40-41	0.14304139126527105
1103	42-43	0.07115041168024305
1103	44-45	0.10511397473356965
1103	46-47	0.12221360873305542
1103	48-49	0.14136869048504508
1103	50-51	0.12423860434657286
1103	52-53	0.16214503660835078
1103	54-55	0.17068534400647195
1103	56-57	0.12083395937069241
1103	58-59	0.18671361294691025
1103	60-61	0.22048644284681984
1103	62-63	0.14662391121231622
1103	64-65	0.1628761072617948
1103	66-67	0.16703687896528407
1103	68-69	0.1800998304899366
1103	70-71	0.2389442597951188
1103	72-73	0.19049814514252006
1103	74-75	0.12573731399474752
1103	76-77	0.10747344150641425
1103	78-79	0.13426354221221004
1103	80-81	0.10368868106845497
1103	82-83	0.06712232765825377
1103	84-85	0.10002219502686671
1103	86-87	0.11344458432663629
1103	88-89	0.09708420057965128
1103	90-91	0.0758376714430824
1103	92-93	0.05141623920252414
1103	94-95	0.11138426287186576
1103	96-97	0.075145417663947
1103	98-99	0.042222790227533835
1103	100	0.09331053095672814
1104	1	0.033659641559523834
1104	2	0.10340117758337897
1104	3	0.0902379882900064
1104	4	0.07711397941354647
1104	5	0.06213438125173809
1104	6	0.003462428087225078
1104	7	0.012950566054868773
1104	8	0.0882520264614115
1104	9	0.07417045823942203
1104	10-11	0.05095102632266446
1104	12-13	0.10481833745537728
1104	14-15	0.09886199803884921
1104	16-17	0.11631133961930828
1104	18-19	0.12070198816651612
1104	20-21	0.08403300388160773
1104	22-23	0.09748512084881611
1104	24-25	0.08022397940301573
1104	26-27	0.0747698833012862
1104	28-29	0.07618211017265253
1104	30-31	0.07135611361478311
1104	32-33	0.06265930901886207
1104	34-35	0.11930007704008005
1104	36-37	0.056484201042898974
1104	38-39	0.06286983841746263
1104	40-41	0.09500221171143153
1104	42-43	0.15493162949692163
1104	44-45	0.1279691902994955
1104	46-47	0.18763943553283013
1104	48-49	0.164329929314313
1104	50-51	0.1049824697912527
1104	52-53	0.18654128797873426
1104	54-55	0.11792923229091912
1104	56-57	0.13529568821691385
1104	58-59	0.22970286683382568
1104	60-61	0.19057918789682304
1104	62-63	0.1610029756537017
1104	64-65	0.20464174627711174
1104	66-67	0.2075077883619798
1104	68-69	0.22982954254268861
1104	70-71	0.26609620761246333
1104	72-73	0.32969864408774185
1104	74-75	0.287660272326697
1104	76-77	0.23062264128382992
1104	78-79	0.2046899815331642
1104	80-81	0.2785492027288612
1104	82-83	0.264472239206043
1104	84-85	0.2410771277948811
1104	86-87	0.2547547775095822
1104	88-89	0.27039836580672016
1104	90-91	0.2677382208434338
1104	92-93	0.3279339940113388
1104	94-95	0.2824671380255168
1104	96-97	0.27577361523213284
1104	98-99	0.2904364864087299
1104	100	0.27563654379682845
1105	1	0.06416118021093098
1105	2	0.05360048908492843
1105	3	0.04457754670413294
1105	4	0.03713622966847652
1105	5	0.04308712108308299
1105	6	0.03472102673086397
1105	7	-0.021686414384731734
1105	8	-0.014707500228780646
1105	9	0.001409241561695751
1105	10-11	0.009886117185040177
1105	12-13	0.010962055690974637
1105	14-15	0.015640404922621087
1105	16-17	0.03660684465305053
1105	18-19	0.02030599533676991
1105	20-21	0.04017252395590276
1105	22-23	0.0965339591214871
1105	24-25	0.006787326622315959
1105	26-27	-0.002982213756155261
1105	28-29	0.08512583761385883
1105	30-31	0.10597001681226459
1105	32-33	0.06330907564156263
1105	34-35	0.07669499908578814
1105	36-37	-0.018937663588154408
1105	38-39	-0.012966088840038026
1105	40-41	0.04436511544304267
1105	42-43	0.040033391915386574
1105	44-45	0.11130925502199318
1105	46-47	0.18227856057531255
1105	48-49	0.1474143740644891
1105	50-51	0.09241397147860653
1105	52-53	0.04784630412395785
1105	54-55	0.0803653620717597
1105	56-57	0.05489837018444632
1105	58-59	0.04347805833067042
1105	60-61	-0.03687725491511884
1105	62-63	0.03517946584922527
1105	64-65	-0.023107998798394647
1105	66-67	-0.04286732746680144
1105	68-69	-0.05059030899413841
1105	70-71	-0.0236254163577172
1105	72-73	0.01932598645942818
1105	74-75	-0.0847283322449428
1105	76-77	-0.0693211893410961
1105	78-79	-0.05356016413797704
1105	80-81	-0.003474042945519784
1105	82-83	0.03962314540191869
1105	84-85	0.00993907454803633
1105	86-87	0.04443018377020991
1105	88-89	0.0033289201258739354
1105	90-91	0.05264656436025916
1105	92-93	0.03935225935131825
1105	94-95	-0.008740344210693252
1105	96-97	0.03168214791911339
1105	98-99	-0.014323280133041294
1105	100	-0.01533788990661833
1106	1	0.004587746006748716
1106	2	0.004414624968283931
1106	3	0.010513297560287072
1106	4	0.04664743149623263
1106	5	-0.024383979224303687
1106	6	0.0028517171570641153
1106	7	0.01012455994776218
1106	8	0.006236191040173367
1106	9	0.025761829990123886
1106	10-11	0.03654867156225805
1106	12-13	0.04451772870185522
1106	14-15	0.07068463740621667
1106	16-17	0.035813473192746414
1106	18-19	0.04359679712361242
1106	20-21	0.02408374965361304
1106	22-23	0.049975921036732984
1106	24-25	0.025811739738131223
1106	26-27	-0.023043203920757094
1106	28-29	-0.04863242553044955
1106	30-31	0.05670663624240291
1106	32-33	0.06288416612816405
1106	34-35	0.07346372603788609
1106	36-37	0.0639815585436807
1106	38-39	0.09776917877135816
1106	40-41	0.0963290888645858
1106	42-43	0.06504995473964215
1106	44-45	0.11781832316826524
1106	46-47	0.16679256346894533
1106	48-49	0.26765164870047187
1106	50-51	0.23733277710412182
1106	52-53	0.14714798622490122
1106	54-55	0.08471864467738044
1106	56-57	0.11753026385110843
1106	58-59	0.1328393665989367
1106	60-61	0.07503860106625382
1106	62-63	0.10891368854126426
1106	64-65	0.1039918872103982
1106	66-67	0.11017402113680674
1106	68-69	0.15197711144341497
1106	70-71	0.07666498110134512
1106	72-73	0.16545777539058548
1106	74-75	0.12001155834298771
1106	76-77	0.03805834046955425
1106	78-79	0.08008733511913135
1106	80-81	0.06645389659043133
1106	82-83	0.13187016043999478
1106	84-85	0.08805562568088021
1106	86-87	0.06980625730914625
1106	88-89	0.04557502177289763
1106	90-91	0.05167818717604433
1106	92-93	0.023759334600121917
1106	94-95	0.01672469361587048
1106	96-97	0.11900196631262361
1106	98-99	0.10955178745789595
1106	100	0.17508015090613327
1107	1	-0.01618321329463157
1107	2	0.01885561731376839
1107	3	0.008692734927542745
1107	4	0.009992879450607006
1107	5	-0.10056573572041572
1107	6	-0.09260529995057709
1107	7	-0.04219584699196588
1107	8	0.017414020354152626
1107	9	-0.06767710348945144
1107	10-11	-0.016502737343387253
1107	12-13	0.004220773313711845
1107	14-15	0.041677982110563505
1107	16-17	0.02391672555021529
1107	18-19	0.03224299984056245
1107	20-21	-0.013778991844851873
1107	22-23	0.027699212712498422
1107	24-25	0.011279713451571638
1107	26-27	0.03514206612529591
1107	28-29	-0.011360620374453845
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2216	4	-0.08969432482498974
2216	5	-0.06153705041044333
2216	6	-0.07831734769841603
2216	7	-0.08351391035439804
2216	8	-0.09459272004225738
2216	9	-0.11188299085652176
2216	10-11	-0.14313532721297406
2216	12-13	-0.12237797046446985
2216	14-15	-0.2503934937760661
2216	16-17	-0.2368235387110431
2216	18-19	-0.1791280467472376
2216	20-21	-0.18881842830081297
2216	22-23	-0.1674427188253631
2216	24-25	-0.2565785970554657
2216	26-27	-0.2391101411251313
2216	28-29	-0.2779834382573796
2216	30-31	-0.24511052116321963
2216	32-33	-0.2472273461722878
2216	34-35	-0.15938008615778188
2216	36-37	-0.15996823557063777
2216	38-39	-0.14342451084726804
2216	40-41	-0.17409591943645353
2216	42-43	-0.16219699276398103
2216	44-45	-0.06475669934892636
2216	46-47	-0.065394099592325
2216	48-49	-0.1265857640391843
2216	50-51	-0.09200710129309186
2216	52-53	-0.03970803695040814
2216	54-55	0.014747310605741859
2216	56-57	-0.030208149984090937
2216	58-59	-0.011067371459994035
2216	60-61	0.014214889318139967
2216	62-63	0.0480800602807534
2216	64-65	0.018854403091737026
2216	66-67	0.038979575974373404
2216	68-69	0.10358112596576063
2216	70-71	0.1036356331952959
2216	72-73	0.06485629790480374
2216	74-75	0.10082164047855002
2216	76-77	0.16057645363807893
2216	78-79	0.070160334568385
2216	80-81	0.07100532725238651
2216	82-83	0.07876711221840793
2216	84-85	0.13434747137497993
2216	86-87	0.05441965747497335
2216	88-89	0.05919496633984522
2216	90-91	0.09508549586936255
2216	92-93	0.067251617117833
2216	94-95	0.060210588806043575
2216	96-97	0.09921482089628597
2216	98-99	0.11391962594648675
2216	100	0.12595573670111548
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	15.0
3	12.0
4	14.0
5	15.0
6	78.0
7	131.0
8	299.0
9	562.0
10	1070.0
11	1715.0
12	2746.0
13	4021.0
14	4974.0
15	6544.0
16	7948.0
17	11395.0
18	13486.0
19	7447.0
20	5057.0
21	4740.0
22	4941.0
23	5410.0
24	6038.0
25	7348.0
26	9240.0
27	11575.0
28	14998.0
29	19288.0
30	24638.0
31	31566.0
32	40658.0
33	53333.0
34	75218.0
35	115740.0
36	215301.0
37	505071.0
38	976252.0
39	527254.0
40	2176.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.55692703905015	20.232292587618794	13.614509338799794	23.596271034531263
2	15.328703455044835	20.591578663908066	40.50276534551552	23.576952535531582
3	17.79172678358718	26.153601092441857	30.57921196741804	25.47546015655292
4	11.950931223000199	17.05351897339042	39.611272751753205	31.384277051856174
5	12.219315729506773	38.239450510950626	36.27815675040703	13.263077009135579
6	30.091306731020378	39.13780988923265	17.778950892282335	12.991932487464638
7	26.40805094566605	33.049308547231796	23.21371942358702	17.328921083515134
8	24.87418644244876	36.59029808248366	20.511509365384644	18.024006109682936
9	25.06400109185366	16.215829450103797	21.58787453836258	37.132294919679964
10-11	23.559110224283312	26.930257590857238	29.398396720306753	20.1122354645527
12-13	24.525974556287462	24.3147038936635	29.64354007667988	21.515781473369156
14-15	21.565662808389913	26.153234065846746	27.055577137576552	25.225525988186785
16-17	20.787168075505626	29.705177547553376	27.591109783490797	21.916544593450205
18-19	20.64322294248156	28.297737087478218	30.254301292236246	20.804738677803975
20-21	21.664789277471254	27.51681740961493	30.00869288831239	20.809700424601427
22-23	21.266321047195728	27.376961583632465	30.095252284739676	21.261465084432132
24-25	21.18008204359251	27.564892317324986	29.979001666843857	21.276023972238644
26-27	20.96785823336166	27.513602401527443	30.054924639568547	21.46361472554235
28-29	21.021452455142516	27.540243933172693	30.098630264997674	21.339673346687118
30-31	21.197932230385483	27.955702006827305	29.73977339470744	21.10659236807977
32-33	20.946827212589035	27.613769857806652	30.170085580948648	21.26931734865566
34-35	21.053772323872202	27.491235123168256	30.138812098151902	21.316180454807643
36-37	21.383794639916662	27.78370824037471	29.548201781935358	21.28429533777327
38-39	21.281863994215495	27.958330956853555	29.608349999356047	21.151455049574903
40-41	21.13376947494467	27.680070330226762	29.784494083826623	21.40166611100194
42-43	21.16166533464622	27.644814908031716	29.610244193936293	21.583275563385776
44-45	21.323571212660045	27.811759545839145	29.459908515074517	21.404760726426293
46-47	21.446889378243583	28.018389434353804	29.1269065976419	21.40781458976071
48-49	21.447127580707175	27.98754969177761	28.952135849639717	21.6131868778755
50-51	21.45948460623087	28.018320909504133	28.81290270599296	21.709291778272036
52-53	21.478201894259	28.011774874956796	28.82664581432921	21.683377416454988
54-55	21.54766275648388	28.349603892573107	28.6079364226794	21.49479692826361
56-57	21.37224836447902	28.08977624182056	28.75321852649888	21.784756867201548
58-59	21.275636127658526	27.7409121861116	29.047766549693314	21.935685136536556
60-61	21.468404666598243	28.091338069386822	28.76619074368989	21.67406652032504
62-63	21.28478420617468	28.105151148107577	28.793381102554832	21.816683543162902
64-65	21.318937182019233	27.856269519850535	28.86919099640887	21.95560230172136
66-67	21.251959641546776	27.94142127193543	28.7534389720304	22.053180114487393
68-69	21.618196362809638	28.050739447507144	28.64745880620444	21.683605383478778
70-71	21.565687315026516	27.897910270960292	28.666811365212386	21.86959104880081
72-73	21.434460107471935	27.999743221016438	28.68046504219011	21.88533162932151
74-75	21.479035092801823	28.056804041507675	28.562617893401832	21.90154297228867
76-77	21.519903542566624	27.999878600484312	28.465666469545738	22.014551387403326
78-79	21.560234116050044	28.072074193156777	28.57267934856584	21.79501234222734
80-81	21.54643288282414	27.932256788217757	28.56890598130556	21.952404347652546
82-83	21.39015193319354	27.985781554648128	28.702553066254644	21.921513445903688
84-85	21.389903666018164	27.886032593490757	28.543152006389278	22.1809117341018
86-87	21.514354896027157	28.049675054979094	28.39847684550599	22.03749320348776
88-89	21.2899691830926	27.837302032547395	28.56324860308581	22.309480181274193
90-91	21.318189133883614	27.81904214471294	28.534173315246132	22.328595406157312
92-93	21.40353226431246	27.939442588105194	28.429838653606115	22.227186493976227
94-95	21.175680955180308	27.700626196973566	28.575065279434238	22.54862756841189
96-97	21.222750572597572	27.738167849630322	28.51701459066171	22.522066987110392
98-99	21.18828067691959	27.766862106438033	28.46911725429807	22.575739962344308
100	21.295442689843778	27.6394853574679	28.42511939385958	22.639952558828742
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	48.0
1	41.0
2	127.0
3	229.0
4	154.5
5	115.0
6	307.5
7	716.0
8	1278.5
9	1524.0
10	1218.0
11	957.0
12	1045.5
13	1208.0
14	1348.0
15	1530.0
16	1782.5
17	2076.0
18	2401.0
19	2799.0
20	3299.0
21	4243.5
22	5714.5
23	7921.0
24	10821.5
25	14455.5
26	19438.5
27	25739.5
28	33986.0
29	42606.5
30	49768.0
31	57200.0
32	65443.0
33	74032.5
34	81294.5
35	86647.0
36	90558.0
37	94271.0
38	97726.0
39	98246.0
40	98335.5
41	98527.0
42	96994.0
43	95788.5
44	93705.5
45	90902.0
46	88648.0
47	85471.0
48	82875.5
49	81459.5
50	79411.0
51	76250.5
52	71174.0
53	66903.5
54	64658.5
55	61725.5
56	57216.5
57	52641.0
58	48034.5
59	42620.0
60	37376.0
61	32034.0
62	27418.5
63	23123.5
64	19007.5
65	15448.5
66	12084.5
67	8952.5
68	6795.5
69	5432.0
70	4022.5
71	2769.5
72	1858.0
73	1373.0
74	1068.0
75	726.5
76	450.0
77	299.5
78	194.5
79	105.5
80	74.0
81	33.5
82	11.5
83	8.0
84	4.5
85	2.5
86	1.5
87	0.5
88	1.0
89	1.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09568431020110259
2	0.0010300502443794206
3	0.0
4	2.5751256109485515E-4
5	0.037743983954760196
6	1.4715003491134579E-4
7	0.004561651082251719
8	2.2072505236701867E-4
9	3.6787508727836446E-5
10-11	4.046625960062009E-4
12-13	0.0
14-15	7.357501745567289E-5
16-17	0.0
18-19	1.1036252618350933E-4
20-21	0.0
22-23	2.391188067309369E-4
24-25	1.1036252618350933E-4
26-27	0.0016186503840248036
28-29	0.00858988328794981
30-31	0.03641963364055808
32-33	0.026174312459855627
34-35	0.02911731315808255
36-37	0.026008768670580364
38-39	0.026082343688036042
40-41	0.031195807401205304
42-43	0.02347043056835965
44-45	0.022017323973610115
46-47	0.016517591418798563
48-49	0.01136734019690146
50-51	0.008001283148304427
52-53	0.005646882589722894
54-55	0.004120200977517682
56-57	0.002464763084765042
58-59	0.0011404127705629298
60-61	6.253876483732195E-4
62-63	0.0013427440685660303
64-65	0.0032189070136856893
66-67	8.645064551041564E-4
68-69	0.0012691690511103574
70-71	6.989626658288925E-4
72-73	8.461127007402382E-4
74-75	7.909314376484836E-4
76-77	6.069938940093014E-4
78-79	1.4715003491134579E-4
80-81	7.173564201928107E-4
82-83	5.150251221897103E-4
84-85	3.8626884164228266E-4
86-87	2.9430006982269157E-4
88-89	3.6787508727836446E-5
90-91	5.5181263091754666E-5
92-93	3.6787508727836446E-5
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2718314.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.767065461819385
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.67442673680125	47.49861696774459
2	14.582296564789424	18.305759261316034
3	4.632477568006257	8.723010682843757
4	1.9670989257003022	4.938761081572219
5	0.9773616815262139	3.0673062322114872
6	0.5578939512891747	2.101041969679242
7	0.34026955805589987	1.4950405137611313
8	0.2428319549309998	1.219347936910051
9	0.168896988459427	0.9541051497843307
>10	0.8099213819256814	8.667438314744432
>50	0.03643986182883057	1.5331901621893587
>100	0.009732996628592176	0.9893114288637366
>500	1.1727668604579308E-4	0.043209735832141605
>1k	1.7591502897187529E-4	0.27799244853781324
>5k	5.8638342990625095E-5	0.18586811400979175
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5050	0.18577691907557403	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4118	0.15149096094123046	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	1.8393754363918223E-5	0.0
22-23	0.0	0.0	0.0	3.6787508727836446E-5	0.0
24-25	0.0	0.0	0.0	1.1036252618350933E-4	0.0
26-27	0.0	0.0	0.0	3.678750872783645E-4	0.0
28-29	0.0	0.0	0.0	9.564752269237476E-4	0.0
30-31	0.0	0.0	0.0	0.004009838451334172	0.0
32-33	0.0	0.0	0.0	0.0124157841956448	0.0
34-35	0.0	0.0	0.0	0.024518874567102993	0.0
36-37	0.0	0.0	0.0	0.045910810892339886	0.0
38-39	0.0	0.0	0.0	0.08297422593563511	0.0
40-41	0.0	0.0	0.0	0.12616276118211509	0.0
42-43	0.0	0.0	0.0	0.17100673432134772	0.0
44-45	0.0	0.0	0.0	0.22284033411886928	0.0
46-47	0.0	0.0	0.0	0.2857101865347418	0.0
48-49	0.0	0.0	0.0	0.35705955971238057	0.0
50-51	0.0	0.0	0.0	0.4357480408812227	0.0
52-53	0.0	0.0	0.0	0.5129650217009514	0.0
54-55	0.0	0.0	0.0	0.5965278477762319	0.0
56-57	0.0	0.0	0.0	0.6981533406368801	0.0
58-59	0.0	0.0	0.0	0.8115876237991637	0.0
60-61	0.0	0.0	0.0	0.9335750027406694	0.0
62-63	0.0	0.0	0.0	1.0594618576073258	0.0
64-65	0.0	0.0	0.0	1.1920072515537203	0.0
66-67	0.0	0.0	0.0	1.3416036557954674	0.0
68-69	0.0	0.0	0.0	1.5087844892091202	0.0
70-71	0.0	0.0	0.0	1.6955546710203455	0.0
72-73	0.0	0.0	0.0	1.8905100735235147	0.0
74-75	0.0	0.0	0.0	2.084361850764849	0.0
76-77	0.0	0.0	0.0	2.294234588057156	0.0
78-79	0.0	0.0	0.0	2.5351927702244845	0.0
80-81	0.0	0.0	0.0	2.7973221636646834	0.0
82-83	0.0	0.0	0.0	3.0708372910561472	0.0
84-85	0.0	0.0	0.0	3.3398275548740877	0.0
86-87	0.0	0.0	0.0	3.6245812661819055	0.0
88	0.0	0.0	0.0	3.860701890951524	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4055	0.0	48.636196	1
GTATCAA	7205	0.0	40.764965	1
TCAACGC	8375	0.0	34.41631	4
ATCAACG	8460	0.0	34.07052	3
CAACGCA	8595	0.0	33.426582	5
AACGCAG	8895	0.0	32.335407	6
TATCAAC	8950	0.0	32.257744	2
ACGCAGA	10330	0.0	27.707022	7
CGCAGAG	10315	0.0	27.563036	8
GTACATG	7430	0.0	26.923815	1
TATACCG	175	5.5333658E-9	26.869436	5
TACATGG	7525	0.0	24.681936	2
GCAGAGT	11565	0.0	24.258816	9
ACATGGG	7570	0.0	23.603498	3
GTGGTAT	2060	0.0	21.249672	1
AGAGTAC	10935	0.0	20.477882	10-11
GAGTACT	7975	0.0	20.240862	12-13
CAGAGTA	11460	0.0	19.908821	10-11
CATGGGG	5075	0.0	19.827425	4
TGGTATC	2100	0.0	19.479984	2
>>END_MODULE