FastQCFastQC Report
Wed 25 May 2016
SRR1033134_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033134_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2005529
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14750.036.7225951
GTATCAA24600.034.2724721
TCAACGC25400.032.3670924
ATCAACG26500.030.8493523
CAACGCA27300.030.2842525
GTACATG55350.028.7624971
AACGCAG28600.028.5713626
TACATGG55000.026.9246882
ACATGGG57500.025.656923
TATCAAC32250.025.510042
ACGCAGA33600.023.6208297
CGCAGAG34000.023.3429368
CATGGGG38300.020.9747474
TCTGTCG5100.020.258178
ACCGCGT1508.338429E-418.784858
GCAGAGT41550.018.310149
CTGTCGC5750.017.9681179
GAGTACA40450.017.4662741
CGCTATA1350.00997945517.4116132
AGAGTAC34850.016.64286610-11
Produced by FastQC (version 0.11.5)