##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033134_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2005529
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.63395842194254	33.0	31.0	34.0	30.0	34.0
2	31.81130265381353	34.0	31.0	34.0	30.0	34.0
3	32.02220411671933	34.0	31.0	34.0	30.0	34.0
4	35.360882839390506	37.0	35.0	37.0	33.0	37.0
5	35.49395895048139	37.0	35.0	37.0	35.0	37.0
6	35.55628116073116	37.0	37.0	37.0	35.0	37.0
7	35.47751042243717	37.0	37.0	37.0	35.0	37.0
8	35.509740322877406	37.0	37.0	37.0	35.0	37.0
9	37.19938978693402	39.0	38.0	39.0	35.0	39.0
10-11	37.1927107511285	39.0	38.0	39.0	35.0	39.0
12-13	37.15696905903629	39.0	38.0	39.0	35.0	39.0
14-15	38.64852191117655	41.0	39.0	41.0	36.0	41.0
16-17	38.66670314914419	41.0	39.0	41.0	36.0	41.0
18-19	38.656783821126496	41.0	39.0	41.0	35.0	41.0
20-21	38.68633961413672	41.0	39.0	41.0	35.0	41.0
22-23	38.65796380904988	41.0	39.0	41.0	35.0	41.0
24-25	38.661077700696424	41.0	39.0	41.0	35.0	41.0
26-27	38.62694057278653	41.0	39.0	41.0	34.5	41.0
28-29	38.6027676488348	41.0	39.0	41.0	34.5	41.0
30-31	38.60273648498725	41.0	39.0	41.0	34.5	41.0
32-33	38.66322675962302	41.0	39.0	41.0	35.0	41.0
34-35	38.64462468505816	41.0	39.0	41.0	35.0	41.0
36-37	38.57273716809879	41.0	39.0	41.0	34.5	41.0
38-39	38.50371049234391	41.0	39.0	41.0	34.0	41.0
40-41	38.447948895279	41.0	39.0	41.0	34.0	41.0
42-43	38.34541485064539	41.0	39.0	41.0	34.0	41.0
44-45	38.23526486029372	40.5	38.0	41.0	33.5	41.0
46-47	38.12070231844067	40.0	38.0	41.0	33.0	41.0
48-49	38.016095005357684	40.0	38.0	41.0	33.0	41.0
50-51	37.90749074184417	40.0	38.0	41.0	33.0	41.0
52-53	37.76887669038942	40.0	38.0	41.0	33.0	41.0
54-55	37.627085422349914	40.0	37.0	41.0	33.0	41.0
56-57	37.48703160113865	40.0	37.0	41.0	32.0	41.0
58-59	37.30420128554611	40.0	37.0	41.0	32.0	41.0
60-61	37.092145763038076	40.0	36.0	41.0	31.5	41.0
62-63	36.839654275754675	39.0	35.5	41.0	31.0	41.0
64-65	36.57552147089371	39.0	35.0	41.0	31.0	41.0
66-67	36.29550308173056	39.0	35.0	41.0	31.0	41.0
68-69	35.98824948430065	38.0	35.0	40.0	31.0	41.0
70-71	35.645640875798854	37.0	35.0	40.0	30.0	41.0
72-73	35.27879352529931	37.0	35.0	39.5	30.0	41.0
74-75	34.91090305849479	36.0	35.0	39.0	30.0	41.0
76-77	34.57526443147918	36.0	34.0	39.0	29.5	40.5
78-79	34.1970976236195	35.5	34.0	37.5	29.0	39.5
80-81	33.82734679977203	35.0	34.0	37.0	29.0	39.0
82-83	33.5948667408948	35.0	34.0	37.0	29.0	39.0
84-85	33.31250308522091	35.0	34.0	36.0	29.0	38.0
86-87	33.04893671445289	35.0	34.0	36.0	29.0	37.0
88-89	32.67811011458822	35.0	34.0	36.0	28.5	37.0
90-91	32.48091850080452	35.0	34.0	35.0	28.0	36.5
92-93	32.299781753342884	35.0	34.0	35.0	28.0	36.0
94-95	32.13209208144086	35.0	34.0	35.0	27.0	36.0
96-97	31.959036244302627	35.0	34.0	35.0	27.0	36.0
98-99	31.775676392612624	35.0	34.0	35.0	27.0	36.0
100	31.62088805497203	35.0	34.0	35.0	26.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.16887486934028217
1101	2	0.21015184448522817
1101	3	0.2757439269449833
1101	4	0.23754976813921047
1101	5	0.2573156200000426
1101	6	0.25612855472024876
1101	7	0.2640321238983745
1101	8	0.28106971243443013
1101	9	0.32849285559922237
1101	10-11	0.3034008466000202
1101	12-13	0.3126405974309776
1101	14-15	0.3240446752479613
1101	16-17	0.2495699084103009
1101	18-19	0.2507160357257092
1101	20-21	0.2585385933740483
1101	22-23	0.21332468292717977
1101	24-25	0.23220341937101807
1101	26-27	0.2586216941542361
1101	28-29	0.33154742447705843
1101	30-31	0.27337152846796187
1101	32-33	0.3436980877266791
1101	34-35	0.32345014682012163
1101	36-37	0.3390747572855872
1101	38-39	0.3264331150535469
1101	40-41	0.28336514952714253
1101	42-43	0.2946584121178333
1101	44-45	0.2935502525169724
1101	46-47	0.25552068658041804
1101	48-49	0.22334971275722637
1101	50-51	0.2508555397544612
1101	52-53	0.23296968373078641
1101	54-55	0.25063243382741973
1101	56-57	0.17867026269785669
1101	58-59	0.2415103876437712
1101	60-61	0.23589670504244964
1101	62-63	0.3262753499790563
1101	64-65	0.32754430146626135
1101	66-67	0.32115759192581805
1101	68-69	0.34813361371594453
1101	70-71	0.3675260912667824
1101	72-73	0.35308247353512456
1101	74-75	0.2998441229563369
1101	76-77	0.13447920653437961
1101	78-79	0.259012304481395
1101	80-81	0.3109684612252437
1101	82-83	0.29000170112167467
1101	84-85	0.1618737303570228
1101	86-87	0.3783308788175219
1101	88-89	0.4440592690802774
1101	90-91	0.4368885911801357
1101	92-93	0.42184810208878787
1101	94-95	0.44376169428253576
1101	96-97	0.44812274788148443
1101	98-99	0.5143344386877118
1101	100	0.5340034458926226
1102	1	0.08492397935924245
1102	2	0.05283780174405095
1102	3	0.15200250923371783
1102	4	0.1127149714451221
1102	5	0.08306340655184385
1102	6	0.08825806674926184
1102	7	0.09577324709678692
1102	8	0.10511484652981551
1102	9	0.17056217656300987
1102	10-11	0.09242656125387327
1102	12-13	0.1623378013995378
1102	14-15	0.1776598205716482
1102	16-17	0.21902156228406255
1102	18-19	0.13638522415871535
1102	20-21	0.13047704018687512
1102	22-23	0.0918924300878956
1102	24-25	0.07341036987445193
1102	26-27	0.10540782259865722
1102	28-29	0.12322743625757226
1102	30-31	0.14740837938924045
1102	32-33	0.12008367868513403
1102	34-35	0.09797846607205685
1102	36-37	0.09765152390255594
1102	38-39	0.011190233981523079
1102	40-41	0.04489716859684734
1102	42-43	0.0454278269576065
1102	44-45	0.007141100529928224
1102	46-47	0.04795446720515173
1102	48-49	0.02162865324721963
1102	50-51	-0.0349958785090152
1102	52-53	-0.06183400504274772
1102	54-55	0.022778880345725838
1102	56-57	-0.06172033497917084
1102	58-59	-0.0654217087355562
1102	60-61	-0.06345720737679272
1102	62-63	0.018158759701655924
1102	64-65	0.004317360902632572
1102	66-67	-0.09855268105079062
1102	68-69	-0.10907182740908894
1102	70-71	-0.05483295672040356
1102	72-73	0.005318930545456624
1102	74-75	0.018489547883170587
1102	76-77	-0.06668227854670761
1102	78-79	-0.03852391139652411
1102	80-81	-0.03678219682692685
1102	82-83	-0.06038484700356861
1102	84-85	-0.15175669282113802
1102	86-87	-0.08512191629356636
1102	88-89	0.06089251958186992
1102	90-91	0.044103235831968846
1102	92-93	0.04013182841272567
1102	94-95	0.04347638495200101
1102	96-97	0.040932032399311424
1102	98-99	0.039243783948816
1102	100	0.015940604211692744
1103	1	0.04648433759468418
1103	2	0.06601978943892917
1103	3	0.10914216112280428
1103	4	0.0961059060716778
1103	5	0.12286858817526536
1103	6	0.10456924703053971
1103	7	0.1193603534293004
1103	8	0.07579898505172622
1103	9	0.2289463827005136
1103	10-11	0.14680445456777136
1103	12-13	0.18646102458237834
1103	14-15	0.21205346541833592
1103	16-17	0.20359246275177867
1103	18-19	0.2065581501433016
1103	20-21	0.2725375757288049
1103	22-23	0.2163459470398692
1103	24-25	0.2237838328153572
1103	26-27	0.2064009170574863
1103	28-29	0.2362824602893454
1103	30-31	0.17355714488320473
1103	32-33	0.2256013958525287
1103	34-35	0.26027878003429805
1103	36-37	0.3068555118487666
1103	38-39	0.2820336685279514
1103	40-41	0.28976761783606264
1103	42-43	0.2712024681090952
1103	44-45	0.20437779020489444
1103	46-47	0.28053009720287037
1103	48-49	0.2564346056578586
1103	50-51	0.221132770201784
1103	52-53	0.1313844157032804
1103	54-55	0.1016398247355852
1103	56-57	0.14212706339774428
1103	58-59	0.16505511600777822
1103	60-61	0.08853414556082129
1103	62-63	0.16382736800591147
1103	64-65	0.24216321668758667
1103	66-67	0.2045686764708492
1103	68-69	0.15200092523740238
1103	70-71	0.1507365024006475
1103	72-73	0.18892604173163363
1103	74-75	0.18257322942746868
1103	76-77	0.1312640186983458
1103	78-79	0.07950370771553139
1103	80-81	0.11719336082363441
1103	82-83	0.11857897076910007
1103	84-85	0.10938283594550313
1103	86-87	0.11757031880111413
1103	88-89	0.21674938312403924
1103	90-91	0.29204704010187754
1103	92-93	0.25449932754654014
1103	94-95	0.2482700963458626
1103	96-97	0.2713113004109289
1103	98-99	0.3101388586948275
1103	100	0.3183400164800787
1104	1	0.09130689024558691
1104	2	0.0563766120840441
1104	3	0.07027509552194999
1104	4	0.09583245237609361
1104	5	0.050868377674937904
1104	6	0.10008332768266115
1104	7	0.04262757424955055
1104	8	0.12051677936241845
1104	9	0.12164728386814971
1104	10-11	0.10186853667076434
1104	12-13	0.12742060114228337
1104	14-15	0.056039525181745375
1104	16-17	0.15045490128002825
1104	18-19	0.1523722675346164
1104	20-21	0.0891467892494262
1104	22-23	0.12175162494190772
1104	24-25	0.15070984932782494
1104	26-27	0.22083924759316886
1104	28-29	0.24962463988251216
1104	30-31	0.25177277099134443
1104	32-33	0.23026965864940507
1104	34-35	0.2072303615163662
1104	36-37	0.26992647554187954
1104	38-39	0.1333347376040237
1104	40-41	0.1671728176859517
1104	42-43	0.22291895564556086
1104	44-45	0.12567169341703988
1104	46-47	0.10290790496000568
1104	48-49	0.0902128750402369
1104	50-51	0.11135623498797997
1104	52-53	0.013125405871249995
1104	54-55	0.05549512838477
1104	56-57	0.049204817201882634
1104	58-59	-0.005076486042334238
1104	60-61	0.018760787524847444
1104	62-63	0.016379266418844907
1104	64-65	-0.029905627739395868
1104	66-67	-0.026465268674144227
1104	68-69	-0.11514649040385194
1104	70-71	-0.17258265494883318
1104	72-73	-0.11559083788627333
1104	74-75	-0.07341963618528524
1104	76-77	-0.11603046191422095
1104	78-79	-0.03799285321078116
1104	80-81	-0.028494874421298277
1104	82-83	-0.05508908595384554
1104	84-85	-0.041765251526236113
1104	86-87	-0.04285035724921471
1104	88-89	0.10884816974703426
1104	90-91	0.1477441478372299
1104	92-93	0.10288462281997113
1104	94-95	0.24396479906579316
1104	96-97	0.19962302877107874
1104	98-99	0.3286524362118115
1104	100	0.351236594423483
1105	1	0.16433809566853697
1105	2	0.20045850853810876
1105	3	0.26148583005477377
1105	4	0.2851027312761545
1105	5	0.2418117813053584
1105	6	0.2989014891794852
1105	7	0.25795321458823395
1105	8	0.2502499319680709
1105	9	0.2788837527083956
1105	10-11	0.2974647710072631
1105	12-13	0.33438528940455825
1105	14-15	0.36513775696709416
1105	16-17	0.440901868271375
1105	18-19	0.3893226888056063
1105	20-21	0.3951096348087759
1105	22-23	0.3990649832417006
1105	24-25	0.4363958386425395
1105	26-27	0.4767284107294856
1105	28-29	0.4508587649933844
1105	30-31	0.4131577155782864
1105	32-33	0.38339414842577924
1105	34-35	0.3844712753606032
1105	36-37	0.4572538462320921
1105	38-39	0.42531596674690064
1105	40-41	0.4173261621485409
1105	42-43	0.36166690569987736
1105	44-45	0.3932518243659686
1105	46-47	0.41690299887151383
1105	48-49	0.467532983766489
1105	50-51	0.45538879960579237
1105	52-53	0.4520225426860378
1105	54-55	0.3658726126993699
1105	56-57	0.33243344106554673
1105	58-59	0.35218025743515113
1105	60-61	0.3117730333590316
1105	62-63	0.2594749115528998
1105	64-65	0.33248208218660835
1105	66-67	0.3876432043723881
1105	68-69	0.3637511275832068
1105	70-71	0.3864480141772617
1105	72-73	0.4111378143572111
1105	74-75	0.49743105302391655
1105	76-77	0.4273696998624317
1105	78-79	0.44629862413056287
1105	80-81	0.39943474642663546
1105	82-83	0.4648277670113501
1105	84-85	0.35641128076801465
1105	86-87	0.5039243754140514
1105	88-89	0.4913189823278685
1105	90-91	0.5048022325707251
1105	92-93	0.47427615199244144
1105	94-95	0.4920080513138103
1105	96-97	0.5161798394491797
1105	98-99	0.4583244495743912
1105	100	0.5344310223552071
1106	1	-0.0552224273823434
1106	2	-0.017842588522395175
1106	3	-0.006145467741248467
1106	4	-0.005727315375338549
1106	5	0.016085565973149585
1106	6	-0.021172662092837413
1106	7	-0.047857174138677294
1106	8	-0.019000358216111124
1106	9	-0.024789108698577422
1106	10-11	-0.10081052030923843
1106	12-13	-0.037167191525888654
1106	14-15	0.018546666220927932
1106	16-17	0.025987389346248335
1106	18-19	0.06421877089731254
1106	20-21	-0.0016586192637930708
1106	22-23	0.03307651201278361
1106	24-25	0.08712576176037601
1106	26-27	0.07740666907377403
1106	28-29	0.14923845156782534
1106	30-31	0.09240972422119853
1106	32-33	0.13831207092906084
1106	34-35	0.1038310895361576
1106	36-37	0.04366184785552463
1106	38-39	0.017793524652738313
1106	40-41	0.08211136812823838
1106	42-43	0.07544528136117634
1106	44-45	0.052775416095691696
1106	46-47	-0.0044880601697485645
1106	48-49	0.06491791807873426
1106	50-51	0.07648702128963691
1106	52-53	0.07049106736035071
1106	54-55	0.025877439481391207
1106	56-57	0.031785144737547455
1106	58-59	0.02591676842219215
1106	60-61	0.0494332674246607
1106	62-63	0.12464234138693797
1106	64-65	0.1167976382789675
1106	66-67	0.14720079893867677
1106	68-69	0.07318628751531975
1106	70-71	0.1338102688306435
1106	72-73	0.1869762512613562
1106	74-75	0.22596042090641077
1106	76-77	0.1560960022276845
1106	78-79	0.18663173869713745
1106	80-81	0.27408411645831166
1106	82-83	0.19458830844574493
1106	84-85	0.10059960430717041
1106	86-87	0.1533014008197071
1106	88-89	0.2656776353226533
1106	90-91	0.20296944304595854
1106	92-93	0.17894275545653215
1106	94-95	0.19478146387090334
1106	96-97	0.19648623575684354
1106	98-99	0.1404477163511224
1106	100	0.18108005002727623
1107	1	0.023592725323961616
1107	2	0.08672716789599733
1107	3	0.08352896890723827
1107	4	0.07329882920473096
1107	5	-0.018063431328378954
1107	6	-0.008924535649292409
1107	7	-0.07257009245067536
1107	8	0.005713844673501001
1107	9	0.03499352792267274
1107	10-11	-0.024778779680872276
1107	12-13	-0.03980758729568379
1107	14-15	-0.023425997539163745
1107	16-17	-0.039417897033196425
1107	18-19	0.09415740448353915
1107	20-21	0.007532426545097337
1107	22-23	0.04634271974268245
1107	24-25	0.02341221057980647
1107	26-27	0.0730070977801418
1107	28-29	-0.008064018931854378
1107	30-31	-0.054036085015596313
1107	32-33	0.025953385171860077
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2216	12-13	-0.173692281099882
2216	14-15	-0.27535639906922427
2216	16-17	-0.32741708669750125
2216	18-19	-0.29502387872225455
2216	20-21	-0.2393812999317504
2216	22-23	-0.19344204028249123
2216	24-25	-0.18978259238009798
2216	26-27	-0.27530165319192434
2216	28-29	-0.2301188734308539
2216	30-31	-0.1952965763578547
2216	32-33	-0.15086548628319463
2216	34-35	-0.1517956923518895
2216	36-37	-0.20167277817671447
2216	38-39	-0.18675800031877543
2216	40-41	-0.19473058024644274
2216	42-43	-0.2093690481176793
2216	44-45	-0.1816753049233526
2216	46-47	-0.11769882321627279
2216	48-49	-0.15305679460566068
2216	50-51	-0.12998805736430086
2216	52-53	-0.11574058251303398
2216	54-55	-0.11694107208501237
2216	56-57	-0.15915762529781574
2216	58-59	-0.20036124743256067
2216	60-61	-0.09775621921450295
2216	62-63	-0.08671725026187005
2216	64-65	-0.03605367773574386
2216	66-67	-0.08012200096451494
2216	68-69	-0.1283020870486169
2216	70-71	-0.17541740259953542
2216	72-73	-0.09432668889081697
2216	74-75	0.003636499483611999
2216	76-77	-0.0700728130344217
2216	78-79	-0.1136045920448936
2216	80-81	-0.06598903318192129
2216	82-83	-0.14039078111645154
2216	84-85	-0.1260432743385067
2216	86-87	-0.14149297741573008
2216	88-89	-0.11786731105100046
2216	90-91	-0.13054908858975267
2216	92-93	-0.07982206778607548
2216	94-95	-0.05416526689946011
2216	96-97	-0.08967686860940915
2216	98-99	-0.12086635045603344
2216	100	-0.08849636972799857
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	16953.0
3	2906.0
4	1969.0
5	1693.0
6	1319.0
7	1617.0
8	1888.0
9	2001.0
10	2259.0
11	2437.0
12	2668.0
13	3103.0
14	3548.0
15	3743.0
16	3689.0
17	3772.0
18	3606.0
19	3541.0
20	3601.0
21	3797.0
22	4538.0
23	6005.0
24	9670.0
25	13575.0
26	13737.0
27	11596.0
28	11994.0
29	15210.0
30	18685.0
31	22739.0
32	30516.0
33	42128.0
34	59685.0
35	90494.0
36	172978.0
37	384811.0
38	684853.0
39	341271.0
40	934.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.99385123192977	20.13690276381897	13.210571418709044	23.658674585542215
2	15.213504966015432	20.564620603410187	40.93743993729766	23.28443449327672
3	17.745992289188848	26.135428667512556	30.33825483397607	25.780324209322526
4	11.913209736320054	17.065795531692125	40.014096584047515	31.00689814794031
5	12.253294683561226	38.43666959483087	36.214507232864875	13.09552848874303
6	29.85888129303213	39.23891942136294	18.277363410465995	12.624835875138944
7	26.58810141908125	32.952574621693024	23.310820581814337	17.148503377411387
8	24.496402142207767	37.19609958204108	20.947715987414853	17.359782288336298
9	24.714602677954726	17.180243039676597	21.542379837890433	36.562774444478244
10-11	23.282979826429646	27.36726690400866	29.635605986364432	19.71414728319727
12-13	23.997127490069644	24.730091286969227	29.58984448051705	21.682936742444078
14-15	21.408728016443547	26.833173454434018	26.373959031271017	25.38413949785142
16-17	21.071132636809693	30.175221436217814	26.868334190709565	21.885311736262924
18-19	20.9814268444487	29.236561617273903	28.738320087454717	21.04369145082268
20-21	22.24818422526529	28.60986690220327	28.124471651072007	21.017477221459433
22-23	21.84779134070509	28.429210545870003	28.19938550414144	21.523612609283465
24-25	21.632918819884775	28.471256081233232	28.18914221974957	21.706682879132426
26-27	21.49209502181995	28.406354503791594	28.23529587781128	21.866254596577182
28-29	21.499390448101064	28.451174631070863	28.29343172607294	21.756003194755134
30-31	21.783217131970837	28.89032685996988	27.915906121268343	21.410549886790946
32-33	21.318832256508763	28.57175631587597	28.433081227791973	21.676330199823298
34-35	21.435838104167082	28.211560710937345	28.574659392391933	21.77794179250364
36-37	21.85939784534431	28.718511448478356	27.94078486754313	21.481305838634203
38-39	21.634694623036577	28.79997307092445	28.092536282565796	21.47279602347318
40-41	21.398863286446083	28.438608367398587	28.459627220786476	21.70290112536886
42-43	21.368772924436964	28.39806509087745	28.387842515491563	21.84531946919402
44-45	21.53943048716543	28.553882610253446	28.346561727143776	21.56012517543735
46-47	21.513760850279976	28.803139381747844	28.179436840113237	21.503662927858947
48-49	21.452029110891154	28.525681460630736	28.358336221318563	21.663953207159544
50-51	21.429465446423716	28.42470278892965	28.324323461785294	21.821508302861332
52-53	21.48193322913033	28.431262795265038	28.34716404193618	21.739639933668446
54-55	21.52261321441759	28.916427132316958	28.12062209370599	21.44033755955947
56-57	21.37989031005684	28.55792978307839	28.188216134763355	21.87396377210142
58-59	21.277580816466962	28.161902197489862	28.581604312206117	21.978912673837055
60-61	21.625516404008067	28.65212632544734	28.0814827557836	21.640874514760988
62-63	21.447811615702015	28.593375634201372	28.124509156184946	21.834303593911667
64-65	21.40254807019871	28.303594379097056	28.386592027787884	21.907265522916354
66-67	21.268356142764862	28.4205857560745	28.198071945882646	22.112986155277987
68-69	21.777601371052572	28.514238457735395	28.06888073173997	21.639279439472062
70-71	21.696182882946445	28.311707998012448	28.162317291201433	21.829791827839674
72-73	21.438896667544256	28.451653662350513	28.3458689356406	21.76358073446463
74-75	21.55991356667605	28.468645730827564	28.175997076006627	21.795443626489764
76-77	21.58927320370292	28.489886284590206	28.01647514641389	21.904365365292993
78-79	21.639237749609627	28.641483871627234	28.12653877059911	21.592739608164027
80-81	21.57494893304788	28.388887212905	28.193196009584852	21.84296784446227
82-83	21.437740981240083	28.448607497734322	28.32634204657506	21.787309474450534
84-85	21.47257083205869	28.394903826567948	28.18213526984134	21.950390071532023
86-87	21.646848458647536	28.501121178531413	28.118918223526652	21.733112139294402
88-89	21.408884541099155	28.388316151202748	28.210048410966465	21.992750896731632
90-91	21.38825218170292	28.31305640103747	28.26007388953096	22.038617527728643
92-93	21.622747758482546	28.46959207417691	28.04762838899227	21.86003177834828
94-95	21.421179414326016	28.27008664314981	28.22817467483737	22.0805592676868
96-97	21.39867965988488	28.25137076646293	28.259396845199262	22.090552728452924
98-99	21.492494863480953	28.361435376169762	28.125927984397826	22.02014177595146
100	21.600785339000993	28.270247710849176	28.05515230740986	22.07381464273998
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	359.0
1	334.5
2	305.5
3	279.5
4	237.0
5	190.0
6	169.5
7	168.5
8	178.0
9	179.5
10	164.5
11	166.5
12	177.5
13	210.0
14	248.0
15	295.0
16	377.5
17	530.0
18	725.5
19	969.5
20	1440.5
21	2201.5
22	3327.0
23	5071.0
24	7377.0
25	10178.5
26	13643.5
27	18151.0
28	23880.5
29	30352.0
30	36472.0
31	42664.5
32	48731.5
33	54766.5
34	60697.0
35	65033.0
36	68491.5
37	71274.0
38	73236.0
39	74223.0
40	74583.0
41	74722.0
42	73665.5
43	72296.5
44	70628.0
45	68480.0
46	66931.0
47	65018.5
48	62933.5
49	61124.5
50	59091.0
51	56694.5
52	53256.5
53	50229.5
54	48058.0
55	45242.5
56	41439.0
57	37380.5
58	33922.0
59	30880.0
60	27387.5
61	23225.0
62	19527.5
63	16284.5
64	13268.5
65	10637.0
66	8280.5
67	6150.0
68	4544.5
69	3413.5
70	2419.0
71	1739.5
72	1382.5
73	1020.0
74	715.0
75	516.5
76	346.5
77	250.0
78	161.5
79	104.5
80	72.0
81	48.0
82	39.0
83	25.0
84	18.5
85	18.5
86	17.5
87	13.0
88	7.5
89	4.5
90	3.0
91	2.5
92	2.0
93	2.0
94	3.0
95	2.0
96	1.0
97	1.0
98	0.5
99	0.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.32869133281044555
2	0.12036724475188341
3	0.06631666757249584
4	0.03929137898280204
5	0.04288145421980934
6	0.009772982589630965
7	0.007329736942223224
8	0.015357544069420088
9	0.017052857375784644
10-11	0.020219104286200797
12-13	0.015656717005837362
14-15	0.018224618043418968
16-17	0.018972550384462154
18-19	0.018798032838218744
20-21	0.02303631610413013
22-23	0.015357544069420088
24-25	0.024307801083903548
26-27	0.018374204511627606
28-29	0.01832434235555806
30-31	0.019396378711053295
32-33	0.02405849030355582
34-35	0.01441016310409872
36-37	0.00982284474570051
38-39	0.013038953812186211
40-41	0.00929929210697028
42-43	0.008152462517370729
44-45	0.009149705638761644
46-47	0.008277117907544594
48-49	0.0046870426705373
50-51	0.006781253225458222
52-53	0.007404530176327542
54-55	0.0037645927832507036
56-57	0.002892005052033653
58-59	0.0029418672081031987
60-61	0.0036897995491463847
62-63	0.00269255642775547
64-65	0.0025180388815120597
66-67	0.0017701065404688737
68-69	0.0031163847543466084
70-71	0.002468176725442514
72-73	0.002044348398851375
74-75	0.0026177631936511515
76-77	0.0041884211098418426
78-79	0.0036150063150420663
80-81	0.003664868471111612
82-83	0.0028172118179293345
84-85	0.0031662469104161545
86-87	0.002493107803477287
88-89	0.6072711987709976
90-91	0.6192131851496538
92-93	0.6156979031467508
94-95	0.6015869129790694
96-97	0.5997170821264614
98-99	0.5964012487478366
100	0.5980965620542011
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2005529.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.28635136896744
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.3859399169522	61.94437231902318
2	11.075271621513375	16.454830383649927
3	2.739501295670121	6.105226674776766
4	1.037434628906088	3.0826893346100803
5	0.530654715899266	1.9710201340446234
6	0.3046123527149861	1.3577124159067981
7	0.19926962517498578	1.0362109375038004
8	0.1471804991460915	0.8746801819381254
9	0.10479282461541986	0.7006208931294894
>10	0.46177193596495786	5.684996014549803
>50	0.011553145185732933	0.5661613144123491
>100	0.001950290785414205	0.19394486006420114
>500	6.714747135372584E-5	0.027534536390873457
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	4.986215606954574E-5	0.0	0.0	0.0	0.0
12-13	4.986215606954574E-5	0.0	0.0	0.0	0.0
14-15	4.986215606954574E-5	0.0	0.0	2.493107803477287E-5	0.0
16-17	4.986215606954574E-5	0.0	0.0	4.986215606954574E-5	0.0
18-19	4.986215606954574E-5	0.0	0.0	9.972431213909148E-5	0.0
20-21	4.986215606954574E-5	0.0	0.0	1.2465539017386435E-4	0.0
22-23	7.479323410431861E-5	0.0	0.0	2.243797023129558E-4	0.0
24-25	9.972431213909148E-5	0.0	0.0	2.7424185838250157E-4	0.0
26-27	9.972431213909148E-5	0.0	0.0	4.986215606954574E-4	0.0
28-29	9.972431213909148E-5	0.0	0.0	0.0016953133063645552	0.0
30-31	9.972431213909148E-5	0.0	0.0	0.005360181777476167	0.0
32-33	9.972431213909148E-5	0.0	0.0	0.015083302211037587	0.0
34-35	1.4958646820863723E-4	0.0	0.0	0.029294016690858123	0.0
36-37	1.4958646820863723E-4	0.0	0.0	0.05173198692215371	0.0
38-39	1.4958646820863723E-4	0.0	0.0	0.09276854136738985	0.0
40-41	1.4958646820863723E-4	0.0	0.0	0.1458468065034213	0.0
42-43	1.4958646820863723E-4	0.0	0.0	0.1972297583330882	0.0
44-45	1.4958646820863723E-4	0.0	0.0	0.2531501663650837	0.0
46-47	1.4958646820863723E-4	0.0	0.0	0.32151118233643095	0.0
48-49	1.4958646820863723E-4	0.0	0.0	0.4005426997066609	0.0
50-51	1.4958646820863723E-4	0.0	0.0	0.4874773688139139	0.0
52-53	1.4958646820863723E-4	0.0	0.0	0.5731156218633587	0.0
54-55	1.4958646820863723E-4	0.0	0.0	0.6668315441960699	0.0
56-57	1.4958646820863723E-4	0.0	0.0	0.7759798038323056	0.0
58-59	1.4958646820863723E-4	0.0	0.0	0.9000119170553007	0.0
60-61	1.4958646820863723E-4	0.0	0.0	1.033542771009544	0.0
62-63	1.4958646820863723E-4	0.0	0.0	1.1645805171603103	0.0
64-65	1.4958646820863723E-4	0.0	0.0	1.3096544602446536	0.0
66-67	1.4958646820863723E-4	0.0	0.0	1.4710582594417732	0.0
68-69	1.4958646820863723E-4	0.0	0.0	1.6510855739308683	0.0
70-71	1.745175462434101E-4	0.0	0.0	1.8520051318131028	0.0
72-73	1.9944862427818296E-4	4.986215606954574E-5	0.0	2.0645924342156112	0.0
74-75	1.9944862427818296E-4	4.986215606954574E-5	0.0	2.2724428317915124	0.0
76-77	1.9944862427818296E-4	4.986215606954574E-5	0.0	2.4923848022142785	0.0
78-79	2.493107803477287E-4	4.986215606954574E-5	0.0	2.739227405836565	0.0
80-81	2.493107803477287E-4	4.986215606954574E-5	0.0	3.0162116828028913	0.0
82-83	2.493107803477287E-4	4.986215606954574E-5	0.0	3.306035464957126	0.0
84-85	2.7424185838250157E-4	4.986215606954574E-5	0.0	3.593066966371466	0.0
86-87	2.9917293641727445E-4	4.986215606954574E-5	0.0	3.8974255670199733	0.0
88	2.9917293641727445E-4	4.986215606954574E-5	0.0	4.139456472581548	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1475	0.0	36.722595	1
GTATCAA	2460	0.0	34.272472	1
TCAACGC	2540	0.0	32.367092	4
ATCAACG	2650	0.0	30.849352	3
CAACGCA	2730	0.0	30.284252	5
GTACATG	5535	0.0	28.762497	1
AACGCAG	2860	0.0	28.571362	6
TACATGG	5500	0.0	26.924688	2
ACATGGG	5750	0.0	25.65692	3
TATCAAC	3225	0.0	25.51004	2
ACGCAGA	3360	0.0	23.620829	7
CGCAGAG	3400	0.0	23.342936	8
CATGGGG	3830	0.0	20.974747	4
TCTGTCG	510	0.0	20.25817	8
ACCGCGT	150	8.338429E-4	18.78485	8
GCAGAGT	4155	0.0	18.31014	9
CTGTCGC	575	0.0	17.968117	9
GAGTACA	4045	0.0	17.466274	1
CGCTATA	135	0.009979455	17.411613	2
AGAGTAC	3485	0.0	16.642866	10-11
>>END_MODULE