FastQCFastQC Report
Wed 25 May 2016
SRR1033134_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033134_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2005529
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38300.1909720577463602No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30310.15113219504679315No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27700.052.3264121
GTATCAA51800.039.7010231
ATCAACG60350.033.577553
TCAACGC60350.033.4996454
CAACGCA62050.032.4294975
AACGCAG63800.031.6002646
TATCAAC64700.031.3215832
ACGCAGA71500.028.1971597
CGCAGAG72600.027.5103158
TACGTCG700.00882364726.8658225
TAGACCG909.5022155E-426.119555
GTACATG53600.025.6370531
TACATGG54500.025.2779462
GCAGAGT81450.024.2903779
ACATGGG56550.023.5289653
TATACCG1201.8346879E-423.5075955
GAGTACT55850.021.70926912-13
ACGTCGC1100.003062119721.3614836
CAGAGTA81050.020.6414510-11
GAGCGTA1405.2168046E-420.1403269
Produced by FastQC (version 0.11.5)