FastQCFastQC Report
Wed 25 May 2016
SRR1033133_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033133_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1102269
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG30500.030.6023651
TACATGG30750.029.1966822
ACATGGG32700.026.7271523
CTGTCGC3800.024.714859
TGCACCG1151.3812352E-424.5110595
TCTGTCG3700.024.1136788
GTCTCGC2653.45608E-1123.1252861
GTATAGA4700.023.0685141
GGTATCA16950.022.5270821
ATCAACG22100.022.3246443
TCAACGC21550.022.236254
CATGGGG19300.021.9075534
GTATACG1303.0966336E-421.7569271
GTATCAA22950.021.361921
CAACGCA22800.021.0171595
GAGTACA21500.020.8293041
AACGCAG23250.020.6010236
TATCAAC25650.019.608382
GCTATAC3204.5838533E-1019.0889023
TCTATAC4500.018.7952233
Produced by FastQC (version 0.11.5)