##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033133_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1102269
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.3693073106474	33.0	31.0	34.0	30.0	34.0
2	31.427995344149206	33.0	31.0	34.0	28.0	34.0
3	31.620399376195827	34.0	31.0	34.0	30.0	34.0
4	34.95988910148067	37.0	35.0	37.0	33.0	37.0
5	35.09237309585954	37.0	35.0	37.0	33.0	37.0
6	35.139565750284184	37.0	35.0	37.0	33.0	37.0
7	35.07667275410993	37.0	35.0	37.0	33.0	37.0
8	35.094570381640054	37.0	35.0	37.0	33.0	37.0
9	36.711077785912515	39.0	38.0	39.0	33.0	39.0
10-11	36.698928755140535	39.0	38.0	39.0	33.0	39.0
12-13	36.63906451147587	39.0	38.0	39.0	33.0	39.0
14-15	38.061262269010555	41.0	39.0	41.0	33.0	41.0
16-17	38.073632207746016	41.0	39.0	41.0	33.0	41.0
18-19	38.064400795087224	41.0	39.0	41.0	33.0	41.0
20-21	38.104599240294334	41.0	39.0	41.0	33.0	41.0
22-23	38.06713560845855	40.0	39.0	41.0	33.0	41.0
24-25	38.06993347358947	40.5	38.5	41.0	33.0	41.0
26-27	38.03989543387322	40.0	38.0	41.0	33.0	41.0
28-29	38.01279859997877	40.0	38.0	41.0	33.0	41.0
30-31	38.00817087299016	40.0	38.0	41.0	33.0	41.0
32-33	38.08053115890948	40.5	39.0	41.0	33.0	41.0
34-35	38.054466287267445	41.0	38.5	41.0	33.0	41.0
36-37	37.9631256072701	41.0	38.0	41.0	33.0	41.0
38-39	37.880049243877856	40.5	38.0	41.0	33.0	41.0
40-41	37.81455479560797	40.0	38.0	41.0	32.0	41.0
42-43	37.691321265498715	40.0	38.0	41.0	32.0	41.0
44-45	37.55439552414157	40.0	38.0	41.0	32.0	41.0
46-47	37.425270510193066	40.0	38.0	41.0	31.0	41.0
48-49	37.309202653798664	40.0	38.0	41.0	31.0	41.0
50-51	37.1924457641465	40.0	37.0	41.0	31.0	41.0
52-53	37.047625398155986	40.0	37.0	41.0	30.5	41.0
54-55	36.9030368267637	40.0	37.0	41.0	30.0	41.0
56-57	36.75465743842928	40.0	36.0	41.0	30.0	41.0
58-59	36.5612105574955	40.0	36.0	41.0	30.0	41.0
60-61	36.34703325594751	39.0	35.5	41.0	30.0	41.0
62-63	36.08402486144489	39.0	35.0	41.0	30.0	41.0
64-65	35.8123393654362	39.0	35.0	41.0	29.5	41.0
66-67	35.524718104201426	38.0	35.0	40.0	29.0	41.0
68-69	35.2162557415658	37.5	35.0	40.0	29.0	41.0
70-71	34.86703926174101	37.0	34.5	40.0	28.5	41.0
72-73	34.501811263856645	36.5	34.0	39.0	28.0	41.0
74-75	34.13764425925069	36.0	34.0	39.0	28.0	41.0
76-77	33.80237537298064	35.5	34.0	39.0	28.0	40.0
78-79	33.429625617703124	35.0	34.0	37.5	27.0	39.5
80-81	33.06404425779914	35.0	34.0	37.0	26.5	39.0
82-83	32.832677867199386	35.0	34.0	37.0	27.0	39.0
84-85	32.55310636514317	35.0	33.5	36.0	26.0	37.5
86-87	32.28754051869372	35.0	33.0	36.0	26.0	37.0
88-89	31.911983372479856	35.0	33.0	35.5	25.5	37.0
90-91	31.711728715948645	35.0	33.0	35.0	25.0	36.0
92-93	31.520826132278057	35.0	33.0	35.0	25.0	36.0
94-95	31.3353160616873	35.0	33.0	35.0	24.0	36.0
96-97	31.149724794945698	35.0	33.0	35.0	23.5	36.0
98-99	30.94542938248286	35.0	33.0	35.0	21.5	35.5
100	30.77821475520041	35.0	33.0	35.0	19.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.2486069534232982
1101	2	0.18098776936101402
1101	3	0.2611735620972979
1101	4	0.2777807342922429
1101	5	0.2443182955306753
1101	6	0.28912272393900196
1101	7	0.24334683041508498
1101	8	0.15063068618712094
1101	9	0.22477253148359466
1101	10-11	0.21032790948438418
1101	12-13	0.24203307142212793
1101	14-15	0.46072341183013066
1101	16-17	0.3287541382708099
1101	18-19	0.3450166713412557
1101	20-21	0.31835460944142113
1101	22-23	0.32879424930295187
1101	24-25	0.22984222506556762
1101	26-27	0.360311678608376
1101	28-29	0.3850171128441531
1101	30-31	0.44835996394152744
1101	32-33	0.43425938791131813
1101	34-35	0.35127371682885666
1101	36-37	0.36670422481072507
1101	38-39	0.35117309206442116
1101	40-41	0.32620592134724546
1101	42-43	0.2545171333762397
1101	44-45	0.3025901415101018
1101	46-47	0.3056825096332787
1101	48-49	0.2600624720435505
1101	50-51	0.24582033722136032
1101	52-53	0.2662832774392996
1101	54-55	0.22429812143212757
1101	56-57	0.3382515522933218
1101	58-59	0.3365362758795172
1101	60-61	0.272359251163266
1101	62-63	0.24414524862746134
1101	64-65	0.2663605513889422
1101	66-67	0.26397115439075947
1101	68-69	0.23559448149170237
1101	70-71	0.27292177173161036
1101	72-73	0.2569798514567765
1101	74-75	0.11976177354683415
1101	76-77	0.3140761232202465
1101	78-79	0.2564244502801287
1101	80-81	0.11609102708273156
1101	82-83	0.17179043150083118
1101	84-85	0.146578101992894
1101	86-87	0.11619091637581391
1101	88-89	0.18882573184946594
1101	90-91	0.30754863104110086
1101	92-93	0.2490498269789434
1101	94-95	0.2146298306995469
1101	96-97	0.2910212721310579
1101	98-99	0.2639830265891874
1101	100	0.32378052052024486
1102	1	0.2478949773429875
1102	2	0.16430960284316853
1102	3	0.25572635317882586
1102	4	0.30188137828390893
1102	5	0.17232246253557548
1102	6	0.17888481231197773
1102	7	0.14868783482561554
1102	8	0.3240382950298297
1102	9	0.3648001311264295
1102	10-11	0.26216010636183995
1102	12-13	0.31200797468808616
1102	14-15	0.31618042944519686
1102	16-17	0.27474204313321593
1102	18-19	0.3473479279367595
1102	20-21	0.3057715737513007
1102	22-23	0.22294657497874226
1102	24-25	0.34021373790480425
1102	26-27	0.43608808767837814
1102	28-29	0.3478049473545255
1102	30-31	0.3719400705519149
1102	32-33	0.3979993424530832
1102	34-35	0.38438229571783467
1102	36-37	0.4311676552839714
1102	38-39	0.44562999468097786
1102	40-41	0.443112901080454
1102	42-43	0.3957775847115812
1102	44-45	0.366500564434034
1102	46-47	0.33891623396154813
1102	48-49	0.3436553976056942
1102	50-51	0.35803267182644305
1102	52-53	0.2075799194827539
1102	54-55	0.251688061091734
1102	56-57	0.28629843786304576
1102	58-59	0.25600349453904414
1102	60-61	0.1657344913072194
1102	62-63	0.23089336129894633
1102	64-65	0.3054084651806477
1102	66-67	0.1623640501885717
1102	68-69	0.15298834462994648
1102	70-71	0.1700471471679208
1102	72-73	0.4442667659790658
1102	74-75	0.3716262714768561
1102	76-77	0.303349975264517
1102	78-79	0.36014803444550836
1102	80-81	0.20457561728214557
1102	82-83	0.3009929506447051
1102	84-85	0.17139156764216068
1102	86-87	0.2501633370268408
1102	88-89	0.303782810346096
1102	90-91	0.39635284945709515
1102	92-93	0.4618165220570063
1102	94-95	0.40132145880788883
1102	96-97	0.436974677439931
1102	98-99	0.4935217472645661
1102	100	0.5756221878633738
1103	1	0.1682219509705689
1103	2	0.21316528618875097
1103	3	0.17458341043272796
1103	4	0.21498457369670376
1103	5	0.11812922812364235
1103	6	0.20546952451956457
1103	7	0.07282533490838006
1103	8	0.17497338034456789
1103	9	0.14549125667185336
1103	10-11	0.11423280432050831
1103	12-13	0.15387898952629087
1103	14-15	0.14159061556839703
1103	16-17	0.2264102657924454
1103	18-19	0.20345317096955284
1103	20-21	0.16993611334302727
1103	22-23	0.256411588320546
1103	24-25	0.15619527125271304
1103	26-27	0.19406855003990842
1103	28-29	0.32929277419572855
1103	30-31	0.30811954601689706
1103	32-33	0.27187993823270773
1103	34-35	0.1957821344905213
1103	36-37	0.22242628773204842
1103	38-39	0.19250497398958544
1103	40-41	0.19179010049249
1103	42-43	0.15146399261999477
1103	44-45	-0.06000651367678955
1103	46-47	0.07474614907718546
1103	48-49	0.15532339679790397
1103	50-51	0.0698080179520204
1103	52-53	0.062272561293269746
1103	54-55	-0.06671933086610693
1103	56-57	0.018398109118230366
1103	58-59	0.03727563432676817
1103	60-61	-0.008696205777447119
1103	62-63	0.013454160880485233
1103	64-65	0.11618342396340609
1103	66-67	0.18904663267273492
1103	68-69	0.1633572141847992
1103	70-71	0.08672747015225468
1103	72-73	0.17500184003768737
1103	74-75	0.06615575753582448
1103	76-77	0.02037141651089769
1103	78-79	0.008186242339355942
1103	80-81	-0.03201139580804835
1103	82-83	0.012469306179070827
1103	84-85	-0.039715446573652
1103	86-87	-0.03900608573302833
1103	88-89	-0.047293597165047885
1103	90-91	-0.0016265948880267445
1103	92-93	0.0822124807801643
1103	94-95	0.006814857426697074
1103	96-97	-0.03575986360847949
1103	98-99	0.04055782973879474
1103	100	0.07220521165350746
1104	1	-0.030821260573315357
1104	2	0.025944474539219442
1104	3	-0.03773118929476027
1104	4	-0.05809509664945267
1104	5	-0.13211116947778834
1104	6	-0.1476202299207401
1104	7	-0.20262049609900856
1104	8	-0.004433458832139081
1104	9	-0.028917953467463064
1104	10-11	0.01473220707802625
1104	12-13	-0.06580660282419615
1104	14-15	-0.07351751833397202
1104	16-17	-0.12437155162542979
1104	18-19	-0.13313048199354682
1104	20-21	-0.13357771327057577
1104	22-23	-0.15480216446308503
1104	24-25	-0.060333039241349695
1104	26-27	-0.03284577717693793
1104	28-29	-0.029346833484986234
1104	30-31	0.01941253096877915
1104	32-33	0.09575643208921036
1104	34-35	-0.017047474330659895
1104	36-37	0.024121375937859568
1104	38-39	-0.05507154207593601
1104	40-41	-0.021610940330084816
1104	42-43	-0.005904899150067422
1104	44-45	-0.023192967376992613
1104	46-47	-0.09187249809356501
1104	48-49	-0.20292965394069995
1104	50-51	-0.1854672237961239
1104	52-53	-0.21434014346486663
1104	54-55	-0.18791273538833764
1104	56-57	-0.2126302884111695
1104	58-59	-0.1661325493958259
1104	60-61	-0.19840202634060233
1104	62-63	-0.2971191318763715
1104	64-65	-0.4242537935469102
1104	66-67	-0.3053152782631088
1104	68-69	-0.3346565794154017
1104	70-71	-0.2242984063536113
1104	72-73	-0.14314892917461464
1104	74-75	-0.12728117785994186
1104	76-77	-0.19156262871146623
1104	78-79	-0.19527116136654143
1104	80-81	-0.013791598570371377
1104	82-83	0.042580531336490424
1104	84-85	-0.14770649093766508
1104	86-87	0.052337799884533354
1104	88-89	0.09790415312157208
1104	90-91	0.050289082877146285
1104	92-93	-0.025386604448435435
1104	94-95	0.004925290262431048
1104	96-97	0.04768781614950157
1104	98-99	0.08759832818116209
1104	100	0.1922611543662498
1105	1	-0.08769279773182603
1105	2	-0.1595079720810837
1105	3	-0.10343526357981858
1105	4	-0.1008131416844904
1105	5	-0.04249720286394876
1105	6	-0.020125244989444013
1105	7	0.0023533992228337297
1105	8	-0.04770311442334929
1105	9	-0.039993030033038224
1105	10-11	-0.13903421762449852
1105	12-13	-0.11185649594260383
1105	14-15	-0.12743039996330907
1105	16-17	-0.034433650114422676
1105	18-19	-0.04850413516324181
1105	20-21	-0.04812827475146264
1105	22-23	0.013052514168307994
1105	24-25	0.08592779697318065
1105	26-27	0.05207310749434413
1105	28-29	0.05161150995368757
1105	30-31	0.1171182897905112
1105	32-33	0.08826720015144929
1105	34-35	0.08977790727517743
1105	36-37	0.1329078515149007
1105	38-39	0.009643994954728896
1105	40-41	0.047215730999134564
1105	42-43	0.11163665494781583
1105	44-45	0.04362673643784376
1105	46-47	0.10169881364536337
1105	48-49	0.059774027411137354
1105	50-51	0.06981208779991732
1105	52-53	0.07021438494947319
1105	54-55	0.051949681583366214
1105	56-57	-0.029052828865424374
1105	58-59	0.01596436034422055
1105	60-61	-0.09338470155779532
1105	62-63	-0.06971589403145373
1105	64-65	-0.021140220806323384
1105	66-67	-0.0024275011182837147
1105	68-69	-0.18947941165547633
1105	70-71	-0.10352163942316395
1105	72-73	-0.1351872373433025
1105	74-75	-0.06317722540298121
1105	76-77	-0.09710261107646545
1105	78-79	-0.11486386446507879
1105	80-81	-0.09185502062388906
1105	82-83	-0.05860729262190745
1105	84-85	-0.001249376120320278
1105	86-87	-0.05157028758352311
1105	88-89	-0.14651610117578429
1105	90-91	-0.06546603512502358
1105	92-93	-0.0861094511650009
1105	94-95	-0.05865581660600583
1105	96-97	0.027197194845001604
1105	98-99	0.02667732279854107
1105	100	0.16631327347953473
1106	1	0.0012418595129481957
1106	2	-0.041106788105448544
1106	3	0.015288487143784835
1106	4	0.019420988918476212
1106	5	-0.06745793190100358
1106	6	0.07392088241753925
1106	7	0.07447548657361835
1106	8	0.015601786455256672
1106	9	0.05366029723753485
1106	10-11	-0.016438941869751034
1106	12-13	-0.05977192190991332
1106	14-15	-0.0012975698003145908
1106	16-17	-0.04337989948857057
1106	18-19	-0.00928982956328639
1106	20-21	-0.0382018351751654
1106	22-23	-0.025154449146334912
1106	24-25	0.02684522604017303
1106	26-27	0.03869056975928231
1106	28-29	0.055999648620463915
1106	30-31	0.06001637281723049
1106	32-33	0.0533910733852494
1106	34-35	-0.02411222960390802
1106	36-37	-0.07827272468126978
1106	38-39	-0.10305633764018296
1106	40-41	-0.09625171311011371
1106	42-43	-0.10759016957261736
1106	44-45	-0.1488833433204988
1106	46-47	-0.18279636939406885
1106	48-49	-0.17935436899768575
1106	50-51	-0.2527130269719393
1106	52-53	-0.29698025095738956
1106	54-55	-0.26840018929942744
1106	56-57	-0.28105529499863025
1106	58-59	-0.37738786842547967
1106	60-61	-0.39247648247740585
1106	62-63	-0.3937108415718171
1106	64-65	-0.4413449547539159
1106	66-67	-0.39370675884549655
1106	68-69	-0.4071078835470985
1106	70-71	-0.39759589638831017
1106	72-73	-0.3359649454106233
1106	74-75	-0.19568301151190326
1106	76-77	-0.24358773770835285
1106	78-79	-0.27158786064351403
1106	80-81	-0.20119374430944958
1106	82-83	-0.2906808537696719
1106	84-85	-0.10872438081763391
1106	86-87	-0.03474739989793818
1106	88-89	-0.08547767518566474
1106	90-91	-0.009491110616863807
1106	92-93	-0.12336663461062614
1106	94-95	-0.14137554302885746
1106	96-97	-0.09457700191492435
1106	98-99	-0.16550095850355717
1106	100	-0.17294729927012753
1107	1	0.3197476618985675
1107	2	0.28955332100748166
1107	3	0.26901965580367104
1107	4	0.31349302557497793
1107	5	0.340993711103728
1107	6	0.28171407555196737
1107	7	0.24720937951133948
1107	8	0.4188172426547965
1107	9	0.34589369452970686
1107	10-11	0.25504505920237364
1107	12-13	0.3591171726116045
1107	14-15	0.25476345203787787
1107	16-17	0.2522407636128534
1107	18-19	0.29460807879829787
1107	20-21	0.3017037652650316
1107	22-23	0.26191863269119864
1107	24-25	0.3024069576654824
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2216	20-21	-0.34870456256491167
2216	22-23	-0.40726284528087575
2216	24-25	-0.44446268849028314
2216	26-27	-0.4895752851669002
2216	28-29	-0.4070679605297727
2216	30-31	-0.5425101360510354
2216	32-33	-0.5004313099113702
2216	34-35	-0.5525398942734014
2216	36-37	-0.5242848886452336
2216	38-39	-0.6085237158244681
2216	40-41	-0.5708314935103047
2216	42-43	-0.5498717537416127
2216	44-45	-0.5290788629210326
2216	46-47	-0.41200852291478185
2216	48-49	-0.4486786417949489
2216	50-51	-0.40236138677366284
2216	52-53	-0.45737635999468296
2216	54-55	-0.41049525206824455
2216	56-57	-0.5207586701130893
2216	58-59	-0.6034230257664817
2216	60-61	-0.5076019897646091
2216	62-63	-0.3979934870670121
2216	64-65	-0.42655681926360955
2216	66-67	-0.5737202500743734
2216	68-69	-0.6257873763934612
2216	70-71	-0.5353662674153199
2216	72-73	-0.4997704434203314
2216	74-75	-0.4137844823108594
2216	76-77	-0.4561023704093401
2216	78-79	-0.4151973472482169
2216	80-81	-0.3801081132575206
2216	82-83	-0.44041163635808545
2216	84-85	-0.3228994607814002
2216	86-87	-0.45534268367519104
2216	88-89	-0.3902870068416
2216	90-91	-0.44294814390656256
2216	92-93	-0.40923010968889173
2216	94-95	-0.37966376595107576
2216	96-97	-0.442804320014055
2216	98-99	-0.39694217026120526
2216	100	-0.4422703577353566
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	15467.0
3	2545.0
4	1538.0
5	1341.0
6	1090.0
7	1146.0
8	1340.0
9	1487.0
10	1605.0
11	1761.0
12	1982.0
13	2262.0
14	2636.0
15	2698.0
16	2611.0
17	2807.0
18	2616.0
19	2587.0
20	2593.0
21	2684.0
22	3102.0
23	4290.0
24	6423.0
25	8550.0
26	8909.0
27	7744.0
28	8171.0
29	10214.0
30	12393.0
31	14839.0
32	19328.0
33	26410.0
34	36530.0
35	54294.0
36	100634.0
37	215513.0
38	351938.0
39	157758.0
40	433.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.676961868611016	19.92053305660996	12.90168127656863	23.5008237982104
2	15.47543246937858	20.10954225687932	40.91675787618818	23.49826739755392
3	18.156613752771126	25.507245324264797	30.008678774586077	26.327462148377993
4	11.762404920492159	16.820848017091393	39.82543760851157	31.591309453904877
5	11.987928843710293	38.22027591214376	35.922762751860596	13.869032492285353
6	30.067703050175744	38.931068459741994	18.075226417799133	12.926002072283122
7	26.739442855691202	32.72324604061724	22.7765216949497	17.760789408741854
8	24.59474982692086	36.99474728630964	20.71714195755909	17.693360929210407
9	24.442549021387023	17.41831281134592	21.572041703340076	36.56709646392697
10-11	23.29297645054524	27.348310069711466	29.191614660959083	20.16709881878421
12-13	24.073012973803323	24.58966042724585	29.319334673495412	22.017991925455412
14-15	21.32729137810024	26.930829805507173	26.381455286703265	25.360423529689317
16-17	20.947388574679625	30.386249037031526	26.61581346223122	22.05054892605763
18-19	20.66315778489475	29.231607520639745	28.765344552318133	21.339890142147368
20-21	22.177727990214294	28.571104489909626	28.0499684441825	21.20119907569358
22-23	21.59764884045787	28.57359328079398	28.181022589260724	21.64773528948743
24-25	21.536336042032477	28.555496572157114	28.170743326935906	21.737424058874506
26-27	21.24389496965916	28.317495604831848	28.473112913287586	21.965496512221403
28-29	21.30605707959884	28.4628070676893	28.423925248909427	21.80721060380243
30-31	21.715405960143514	29.099027815333912	27.86206483529845	21.323501389224123
32-33	21.00415330853629	28.635415371687994	28.67820071088091	21.682230608894816
34-35	21.19885873098471	28.204306934039497	28.716190313731023	21.880644021244773
36-37	21.85061623999245	28.81171472951492	27.942155823794558	21.395513206698073
38-39	21.571735508995292	28.856842918997	28.240038398304378	21.33138317370333
40-41	21.053166408313043	28.634518312259026	28.550729174180244	21.761586105247684
42-43	21.058130250957195	28.529913444265002	28.493077355785807	21.918878948992
44-45	21.44082309417905	28.61105125512336	28.44955349953388	21.498572151163714
46-47	21.341565484844843	29.133650733884814	28.146518385113172	21.37826539615717
48-49	21.18040970079449	28.705774442284156	28.4580015405287	21.655814316392657
50-51	21.137187194361832	28.447462443363374	28.613675170250097	21.801675192024693
52-53	21.22819520511126	28.542194685239313	28.464622169680574	21.76498793996885
54-55	21.420296087429076	29.088984088793566	28.19235703230325	21.298362791474105
56-57	21.16433869691757	28.655121170553272	28.23076113542506	21.9497789971041
58-59	21.034116734482804	28.22856987330403	28.733856810418736	22.003456581794428
60-61	21.48055307198455	28.808017466195086	28.145142157789998	21.56628730403037
62-63	21.22211043058329	28.789093934310667	28.23486344736988	21.753932187736165
64-65	21.153410856405404	28.482765972406504	28.556251856992837	21.80757131419525
66-67	21.08552822574792	28.519781248639152	28.185920512113338	22.20877001349959
68-69	21.69021201931168	28.746514466749133	28.137932536134553	21.425340977804634
70-71	21.662214395781707	28.44513455021177	28.229030439198493	21.66362061480803
72-73	21.303707440146642	28.574247464179166	28.583047593731585	21.538997501942607
74-75	21.28618352263629	28.63554444087093	28.404017072421706	21.67425496407107
76-77	21.35014642962124	28.732957370290933	28.11299032149195	21.803905878595874
78-79	21.649304957023645	28.841607136052783	28.122794252400123	21.38629365452345
80-81	21.444756084118215	28.51906740353346	28.35680610614166	21.679370406206665
82-83	21.181886802038747	28.602462608165506	28.58191365629814	21.633736933497605
84-85	21.25116693868775	28.588750393515767	28.37713643906108	21.782946228735405
86-87	21.50717374530335	28.72609942313371	28.170507830130866	21.596219001432075
88-89	21.23177349220962	28.59777696738113	28.26217234276492	21.90827719764433
90-91	21.13520693448482	28.417831010731287	28.418013593270008	22.028948461513885
92-93	21.446913665899245	28.799333967476144	28.10769534124125	21.64605702538336
94-95	21.285778766203332	28.40682403078947	28.385328361185667	21.922068841821538
96-97	21.142418507391575	28.460426677303097	28.500815302588382	21.896339512716942
98-99	21.19735059928225	28.577713198812756	28.286651503590583	21.938284698314416
100	21.40862810958329	28.55317381720602	28.156315460407722	21.881882612802972
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	411.0
1	347.0
2	249.5
3	207.5
4	173.5
5	134.5
6	107.0
7	111.0
8	124.5
9	126.0
10	123.5
11	123.5
12	124.5
13	131.5
14	145.5
15	187.0
16	249.5
17	334.5
18	482.0
19	665.0
20	944.5
21	1396.5
22	2190.5
23	3321.5
24	4647.0
25	6205.0
26	8306.0
27	11068.0
28	14396.0
29	17924.5
30	21018.5
31	24129.5
32	27318.5
33	30166.5
34	32823.5
35	35134.5
36	37190.0
37	38940.5
38	39810.5
39	40037.0
40	40427.5
41	40248.5
42	39270.0
43	38320.5
44	37365.5
45	36351.5
46	35442.5
47	34789.5
48	34965.5
49	34493.5
50	32997.0
51	31464.5
52	29010.5
53	26902.0
54	25814.0
55	24856.0
56	23125.5
57	21061.5
58	19316.0
59	17371.5
60	15329.0
61	13064.0
62	10860.5
63	8858.5
64	7096.0
65	5687.5
66	4434.0
67	3276.0
68	2338.5
69	1725.5
70	1218.5
71	853.5
72	652.0
73	506.5
74	410.0
75	317.5
76	225.0
77	140.0
78	92.5
79	74.5
80	53.5
81	31.5
82	24.0
83	21.5
84	15.5
85	13.0
86	10.0
87	5.5
88	5.5
89	4.5
90	2.0
91	2.5
92	2.5
93	1.0
94	1.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3355805161897867
2	0.12011587008252976
3	0.0663177500229073
4	0.038828997277434094
5	0.04254859748391727
6	0.009344361494335774
7	0.006985590631687909
8	0.015604176475978187
9	0.018053669294881742
10-11	0.020548523091913136
12-13	0.014833039847804848
14-15	0.017917586360498207
16-17	0.018870166901182924
18-19	0.018915527879310768
20-21	0.022998015910816686
22-23	0.015830981366617404
24-25	0.023950596451501403
26-27	0.018734083966799393
28-29	0.019187693748077828
30-31	0.01950522059497273
32-33	0.023224820801455905
34-35	0.014742317891549159
36-37	0.0091629175818244
38-39	0.012428908007029138
40-41	0.008845390734929496
42-43	0.008074254106756154
44-45	0.007847449216116936
46-47	0.007847449216116936
48-49	0.0045360978127843565
50-51	0.006078371069131038
52-53	0.006758785741048692
54-55	0.0024494928189035528
56-57	0.00190516108136943
58-59	0.0021773269501364914
60-61	0.002313409884520022
62-63	0.0018144391251137427
64-65	0.0014969122782188377
66-67	0.0011793854313239328
68-69	0.003129907490821206
70-71	0.002041244015752961
72-73	0.0011340244531960891
74-75	0.0021319659720086476
76-77	0.0029938245564376754
78-79	0.00317526846894905
80-81	0.003129907490821206
82-83	0.0020866049938808038
84-85	0.0023587708626478655
86-87	0.0018598001032415863
88-89	0.6154123902604536
90-91	0.6236227273015933
92-93	0.6193587953575761
94-95	0.6077917459349759
96-97	0.6045257555097712
98-99	0.6010329601939273
100	0.6045711164878991
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1102269.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.7827797027936
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.23461472658603	70.47054663915875
2	9.10868573798958	14.716499067079807
3	1.9652716712698934	4.762803254290104
4	0.6723848918748314	2.1726848238324483
5	0.31794106301330427	1.284208142593789
6	0.19663018390351095	0.9530599697518272
7	0.11310228555451204	0.6395701912482821
8	0.08239226520369393	0.5324700967331324
9	0.05563941741147416	0.4045236119588606
>10	0.24332215879000121	3.272306297135155
>50	0.007544074822083284	0.4152490172444071
>100	0.002246841645351219	0.2652705057964348
>500	2.2468193569960483E-4	0.11080838317696644
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	9.072195625568713E-5	0.0
10-11	0.0	0.0	0.0	9.072195625568713E-5	0.0
12-13	0.0	0.0	0.0	9.072195625568713E-5	0.0
14-15	0.0	0.0	0.0	1.360829343835307E-4	0.0
16-17	0.0	0.0	0.0	2.721658687670614E-4	0.0
18-19	0.0	0.0	0.0	2.721658687670614E-4	0.0
20-21	0.0	0.0	0.0	3.628878250227485E-4	0.0
22-23	0.0	0.0	0.0	4.989707594062792E-4	0.0
24-25	0.0	0.0	0.0	7.711366281733406E-4	0.0
26-27	0.0	0.0	0.0	0.001043302496940402	0.0
28-29	0.0	0.0	0.0	0.001587634234474525	0.0
30-31	0.0	0.0	0.0	0.005670122265980446	0.0
32-33	0.0	0.0	0.0	0.013880459307120131	0.0
34-35	0.0	0.0	0.0	0.022816571998305314	0.0
36-37	0.0	0.0	0.0	0.04050735346816431	0.0
38-39	0.0	0.0	0.0	0.06854043795117162	0.0
40-41	0.0	0.0	0.0	0.11186017206326224	0.0
42-43	0.0	0.0	0.0	0.15645001356293248	0.0
44-45	0.0	0.0	0.0	0.2021738795157988	0.0
46-47	0.0	0.0	0.0	0.2538853945815404	0.0
48-49	0.0	0.0	0.0	0.31779901276367206	0.0
50-51	0.0	0.0	0.0	0.3921456559152076	0.0
52-53	0.0	0.0	0.0	0.46213764516647027	0.0
54-55	0.0	0.0	0.0	0.536166761471111	0.0
56-57	0.0	0.0	0.0	0.6197670441607266	0.0
58-59	0.0	0.0	0.0	0.7189715033263205	0.0
60-61	0.0	0.0	0.0	0.8241636116047897	0.0
62-63	0.0	0.0	0.0	0.9320320175928017	0.0
64-65	0.0	0.0	0.0	1.0463416824749676	0.0
66-67	0.0	0.0	0.0	1.1792039874114213	0.0
68-69	0.0	0.0	0.0	1.321047766017188	0.0
70-71	0.0	0.0	0.0	1.4833039847804845	0.0
72-73	0.0	0.0	0.0	1.6651561460950095	0.0
74-75	0.0	0.0	0.0	1.8384804435214996	0.0
76-77	0.0	0.0	0.0	2.022237765917394	0.0
78-79	0.0	0.0	0.0	2.232485899539949	0.0
80-81	0.0	0.0	0.0	2.454618609431999	0.0
82-83	0.0	0.0	0.0	2.6938524080782456	0.0
84-85	0.0	0.0	0.0	2.9345831190027116	0.0
86-87	0.0	0.0	0.0	3.175404551883433	0.0
88	0.0	0.0	0.0	3.3774877094429763	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACATG	3050	0.0	30.602365	1
TACATGG	3075	0.0	29.196682	2
ACATGGG	3270	0.0	26.727152	3
CTGTCGC	380	0.0	24.71485	9
TGCACCG	115	1.3812352E-4	24.511059	5
TCTGTCG	370	0.0	24.113678	8
GTCTCGC	265	3.45608E-11	23.125286	1
GTATAGA	470	0.0	23.068514	1
GGTATCA	1695	0.0	22.527082	1
ATCAACG	2210	0.0	22.324644	3
TCAACGC	2155	0.0	22.23625	4
CATGGGG	1930	0.0	21.907553	4
GTATACG	130	3.0966336E-4	21.756927	1
GTATCAA	2295	0.0	21.36192	1
CAACGCA	2280	0.0	21.017159	5
GAGTACA	2150	0.0	20.829304	1
AACGCAG	2325	0.0	20.601023	6
TATCAAC	2565	0.0	19.60838	2
GCTATAC	320	4.5838533E-10	19.088902	3
TCTATAC	450	0.0	18.795223	3
>>END_MODULE