FastQCFastQC Report
Wed 25 May 2016
SRR1033133_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033133_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1102269
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23740.21537392415100126No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21650.19641303529356266No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11360.10306014230646059No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA39050.033.262091
GGTATCA25250.032.6165581
GTCTCGC2850.028.0714911
ATCAACG45900.027.5491433
CAACGCA46350.027.4845145
TCAACGC46200.027.4720024
CAGCGCG700.00884254626.8529829
AACGCAG48300.026.3701026
TATCAAC48600.025.9219132
GTACATG30500.024.3791581
ACGCAGA52250.024.1966747
CGCAGAG52400.024.0344438
TATACTG6300.023.8768985
TACATGG30550.023.0806772
GAGTACT33750.022.69574412-13
TCTGTCG2756.002665E-1122.214748
ACACCGT1503.2916934E-521.932926
ACTCCGC1100.003062215621.3603278
AGAGTAC47200.021.355810-11
ACATGGG32500.021.261923
Produced by FastQC (version 0.11.5)