##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033133_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1102269
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.71694477482357	34.0	31.0	34.0	31.0	34.0
2	32.81825126171561	34.0	31.0	34.0	31.0	34.0
3	32.918705869438405	34.0	31.0	34.0	31.0	34.0
4	36.34912530425876	37.0	37.0	37.0	35.0	37.0
5	36.291075953329	37.0	37.0	37.0	35.0	37.0
6	36.38390175175025	37.0	37.0	37.0	35.0	37.0
7	36.35614718367295	37.0	37.0	37.0	35.0	37.0
8	36.35282131675662	37.0	37.0	37.0	35.0	37.0
9	38.16683586311508	39.0	39.0	39.0	37.0	39.0
10-11	38.14947893844425	39.0	39.0	39.0	37.0	39.0
12-13	38.10764069387781	39.0	39.0	39.0	37.0	39.0
14-15	39.631282382068264	41.0	40.0	41.0	37.0	41.0
16-17	39.64883254450592	41.0	40.0	41.0	37.0	41.0
18-19	39.67033999867546	41.0	40.0	41.0	37.0	41.0
20-21	39.66617041756595	41.0	40.0	41.0	37.0	41.0
22-23	39.59099366851467	41.0	40.0	41.0	37.0	41.0
24-25	39.539102070365765	41.0	40.0	41.0	37.0	41.0
26-27	39.432361791903794	41.0	39.0	41.0	36.0	41.0
28-29	39.33835116473384	41.0	39.0	41.0	36.0	41.0
30-31	39.239228355328876	41.0	39.0	41.0	36.0	41.0
32-33	39.25787716065679	41.0	39.0	41.0	35.5	41.0
34-35	39.19253195000495	41.0	39.0	41.0	35.0	41.0
36-37	39.098127589544845	41.0	39.0	41.0	35.0	41.0
38-39	39.00201947074625	41.0	39.0	41.0	35.0	41.0
40-41	38.88597656288982	41.0	39.0	41.0	35.0	41.0
42-43	38.77243440575758	41.0	39.0	41.0	35.0	41.0
44-45	38.62445237959155	41.0	39.0	41.0	35.0	41.0
46-47	38.40293521817269	41.0	38.5	41.0	35.0	41.0
48-49	38.20430675270737	41.0	38.0	41.0	34.0	41.0
50-51	38.07216160483512	40.5	38.0	41.0	34.0	41.0
52-53	37.94131650259601	40.0	38.0	41.0	34.0	41.0
54-55	37.80884248763233	40.0	38.0	41.0	34.0	41.0
56-57	37.662383229502055	40.0	37.0	41.0	33.0	41.0
58-59	37.4846629996852	40.0	37.0	41.0	33.0	41.0
60-61	37.296755601400385	40.0	36.5	41.0	33.0	41.0
62-63	37.06663119438177	39.5	36.0	41.0	33.0	41.0
64-65	36.81922969801383	39.0	35.5	41.0	32.0	41.0
66-67	36.54668597229896	39.0	35.0	41.0	32.0	41.0
68-69	36.23114457541671	38.5	35.0	41.0	32.0	41.0
70-71	35.8905952176828	37.0	35.0	40.0	31.0	41.0
72-73	35.54227234912712	37.0	35.0	39.5	31.0	41.0
74-75	35.20848223074404	36.5	35.0	39.0	31.0	41.0
76-77	34.86744569610504	36.0	35.0	39.0	31.0	41.0
78-79	34.522511746225284	35.5	35.0	38.0	31.0	39.5
80-81	34.17747754858387	35.0	35.0	37.0	30.0	39.0
82-83	33.84335357340177	35.0	34.0	37.0	30.0	39.0
84-85	33.56231600453247	35.0	34.0	36.0	30.0	38.0
86-87	33.31722565000014	35.0	34.0	36.0	30.0	37.0
88-89	33.08993811855364	35.0	34.0	36.0	29.5	37.0
90-91	32.88121229935705	35.0	34.0	35.0	29.0	36.5
92-93	32.70405908176679	35.0	34.0	35.0	29.0	36.0
94-95	32.546212857297085	35.0	34.0	35.0	29.0	36.0
96-97	32.40880266069353	35.0	34.0	35.0	29.0	36.0
98-99	32.297215108108816	35.0	34.0	35.0	29.0	36.0
100	32.190402705691625	35.0	34.0	35.0	29.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.08877856581507615
1101	2	0.10695164290062564
1101	3	0.09190983920255746
1101	4	0.08253055554277466
1101	5	0.022410280102640456
1101	6	0.07183974372370727
1101	7	0.13212413132451672
1101	8	0.11814916801554176
1101	9	0.16852924501665
1101	10-11	0.1890416485526245
1101	12-13	0.22055836816169716
1101	14-15	0.25162145229663224
1101	16-17	0.20892772770581303
1101	18-19	0.27142660050429157
1101	20-21	0.16720108225459285
1101	22-23	0.2244725935547791
1101	24-25	0.20064080000407358
1101	26-27	0.1886821528287257
1101	28-29	0.1868491303888007
1101	30-31	0.0702421011187937
1101	32-33	0.08833822579985195
1101	34-35	0.04940082314177374
1101	36-37	0.16057814909084556
1101	38-39	0.05909958785595393
1101	40-41	0.13085885685531196
1101	42-43	0.057391595282823005
1101	44-45	-0.07531341650453527
1101	46-47	-0.021076314869695523
1101	48-49	-0.06072622568682107
1101	50-51	-0.07537379423462909
1101	52-53	-0.03045707549399168
1101	54-55	-0.011799488260813007
1101	56-57	0.042397980192284024
1101	58-59	-0.09721322822208833
1101	60-61	-0.03100874701270584
1101	62-63	0.025141798524138892
1101	64-65	-0.00525811214946259
1101	66-67	0.014786333751459324
1101	68-69	0.02074055552024845
1101	70-71	0.07298574105214328
1101	72-73	0.1092952057688521
1101	74-75	0.14756074986585332
1101	76-77	0.10858013592416427
1101	78-79	0.14248378728948552
1101	80-81	0.16067378506166108
1101	82-83	0.12910482078985552
1101	84-85	0.1627980143700256
1101	86-87	0.175560621583692
1101	88-89	0.26171018872114615
1101	90-91	0.3593528324304458
1101	92-93	0.19911446263755295
1101	94-95	0.1227726256707129
1101	96-97	0.18782902815916458
1101	98-99	0.19007811984327105
1101	100	0.2613701461854916
1102	1	0.07030439312789838
1102	2	0.15400125461152925
1102	3	0.12189739230480967
1102	4	0.08112520608258933
1102	5	0.052763122595266054
1102	6	0.07966133367960282
1102	7	0.12137177047271308
1102	8	0.11468568342534269
1102	9	0.15525029921477085
1102	10-11	0.1175159565321735
1102	12-13	0.15993047628736434
1102	14-15	0.2122877333800517
1102	16-17	0.1292971773364613
1102	18-19	0.15994399910266566
1102	20-21	0.2296486564624587
1102	22-23	0.20051823443472472
1102	24-25	0.1577218333671695
1102	26-27	0.2791394748516183
1102	28-29	0.22282421629945048
1102	30-31	0.21999975866675925
1102	32-33	0.16396750442094543
1102	34-35	0.18423404147659284
1102	36-37	0.17584335595287826
1102	38-39	0.2544389349349956
1102	40-41	0.2931521977650178
1102	42-43	0.3744467201576711
1102	44-45	0.4097621442823254
1102	46-47	0.3788749798782547
1102	48-49	0.46527696721304324
1102	50-51	0.42876699776689264
1102	52-53	0.36013621535754226
1102	54-55	0.3253051197971999
1102	56-57	0.4302473727295606
1102	58-59	0.4402111771679529
1102	60-61	0.4189787384433714
1102	62-63	0.32731560862777087
1102	64-65	0.33033883159596655
1102	66-67	0.33595688330905205
1102	68-69	0.36746698758994967
1102	70-71	0.2651922242623641
1102	72-73	0.3111353510728492
1102	74-75	0.3161925381603581
1102	76-77	0.2609531640379146
1102	78-79	0.3569027403971532
1102	80-81	0.29824498266969357
1102	82-83	0.18619989073601317
1102	84-85	0.10198916895507182
1102	86-87	0.10177301030572039
1102	88-89	0.1282662538779462
1102	90-91	0.02565505528403378
1102	92-93	0.15665887235695664
1102	94-95	0.16799579042974955
1102	96-97	0.2118679450084784
1102	98-99	0.15549147184695045
1102	100	0.12353766519406406
1103	1	0.11400626495383648
1103	2	0.16564264467569245
1103	3	0.15129240915587872
1103	4	0.07780589323538578
1103	5	0.0869701291966507
1103	6	0.11669685334801017
1103	7	0.11983646930539038
1103	8	0.1247234907338779
1103	9	0.12657368743822417
1103	10-11	0.11119597824985306
1103	12-13	0.130501525556042
1103	14-15	0.20157062772484835
1103	16-17	0.18523614435603974
1103	18-19	0.18487753835671583
1103	20-21	0.1618752487063304
1103	22-23	0.14270255830629708
1103	24-25	0.16121888525787398
1103	26-27	0.13392626248842276
1103	28-29	0.11852864227062554
1103	30-31	0.12502896663343677
1103	32-33	0.15196629107781945
1103	34-35	0.18361309285084104
1103	36-37	0.18196366231787664
1103	38-39	0.20569560583926005
1103	40-41	0.24112162747719168
1103	42-43	0.21782593044179066
1103	44-45	0.2808460917361302
1103	46-47	0.24822374733351893
1103	48-49	0.2961248507320988
1103	50-51	0.2598306924877676
1103	52-53	0.3174447041245969
1103	54-55	0.17554911267273354
1103	56-57	0.2697260490102735
1103	58-59	0.294708699167046
1103	60-61	0.272208498952935
1103	62-63	0.18122570205353838
1103	64-65	0.321203789650383
1103	66-67	0.27533938600720376
1103	68-69	0.295879122014469
1103	70-71	0.2833501608175979
1103	72-73	0.31192721058327777
1103	74-75	0.4221076273154196
1103	76-77	0.3020023996151906
1103	78-79	0.20859492368644794
1103	80-81	0.2687765113827183
1103	82-83	0.22500079983294796
1103	84-85	0.25013662739021925
1103	86-87	0.22564305347624014
1103	88-89	0.18855061979442667
1103	90-91	0.21058539151084688
1103	92-93	0.12819269202072547
1103	94-95	0.20165944111935374
1103	96-97	0.2898378630374623
1103	98-99	0.2166541872857053
1103	100	0.2066351166768925
1104	1	0.09962802738938592
1104	2	0.10311765953171914
1104	3	0.05681405094244241
1104	4	0.058706138735061586
1104	5	0.02133588169215983
1104	6	0.050528388951462944
1104	7	0.05137867544107877
1104	8	0.10837165188730324
1104	9	0.052144669256847465
1104	10-11	0.08156194683309792
1104	12-13	0.06958699425633341
1104	14-15	0.08121156194414425
1104	16-17	0.07548278449193901
1104	18-19	0.15353161191057296
1104	20-21	0.05148923198354538
1104	22-23	0.030673074335808792
1104	24-25	0.08595612857865831
1104	26-27	0.003047325640068266
1104	28-29	0.09938807119876714
1104	30-31	0.08503592946034644
1104	32-33	0.12305135161239633
1104	34-35	0.07155599767019538
1104	36-37	0.0580307455536655
1104	38-39	0.008165008416710862
1104	40-41	0.011704884279453154
1104	42-43	0.02453965621445775
1104	44-45	0.1344622599003955
1104	46-47	0.06604378226769114
1104	48-49	0.09411163336584849
1104	50-51	0.12418221920761852
1104	52-53	0.005875006827494644
1104	54-55	0.12119104329656949
1104	56-57	0.05481059333526872
1104	58-59	0.08494513516005497
1104	60-61	0.14214049134184847
1104	62-63	0.16949814066361313
1104	64-65	0.22711979154715323
1104	66-67	0.2674563855090071
1104	68-69	0.24759131716569982
1104	70-71	0.1653680600846954
1104	72-73	0.2222063839276558
1104	74-75	0.26170270291611075
1104	76-77	0.27203403245812297
1104	78-79	0.29627852322786197
1104	80-81	0.25322719836163543
1104	82-83	0.25011139473446775
1104	84-85	0.2799675387402232
1104	86-87	0.3577704972183966
1104	88-89	0.2896028469986689
1104	90-91	0.29894306472617416
1104	92-93	0.31503174867773254
1104	94-95	0.3256229703122102
1104	96-97	0.3144418522570902
1104	98-99	0.41994112178111465
1104	100	0.44868218778776736
1105	1	-0.050443885029743285
1105	2	0.03066904636037293
1105	3	-0.016829906440285924
1105	4	0.007177365307121875
1105	5	0.06632165901370968
1105	6	-3.064785453688046E-5
1105	7	-0.052556860950367934
1105	8	-0.12176200180880414
1105	9	-0.02558203346943344
1105	10-11	-0.014134212385698675
1105	12-13	-0.06381026122733857
1105	14-15	-0.06376368702323276
1105	16-17	-0.0013386677817166515
1105	18-19	-0.026989052804580638
1105	20-21	0.011792824596398077
1105	22-23	0.06298102117514048
1105	24-25	-0.025233443838558856
1105	26-27	-0.13428020205796543
1105	28-29	-0.10436491105155454
1105	30-31	-0.10091755401483482
1105	32-33	-0.07405523105304468
1105	34-35	-0.09476605340360322
1105	36-37	-0.17502227034978546
1105	38-39	-0.20860308766204838
1105	40-41	-0.06728446892238793
1105	42-43	-0.2043281508985757
1105	44-45	-0.14616312433752654
1105	46-47	-0.19348672833867653
1105	48-49	-0.3013294012705572
1105	50-51	-0.41022538226409466
1105	52-53	-0.2716588014098136
1105	54-55	-0.31233650057000517
1105	56-57	-0.43732397407158174
1105	58-59	-0.41916994727131396
1105	60-61	-0.47266068256963933
1105	62-63	-0.4642967481964462
1105	64-65	-0.6027863744962687
1105	66-67	-0.5892153009265257
1105	68-69	-0.6444270623325892
1105	70-71	-0.5189296516903283
1105	72-73	-0.44421079834635435
1105	74-75	-0.4526030743302343
1105	76-77	-0.4706781843808514
1105	78-79	-0.4546365975567994
1105	80-81	-0.39579883487412104
1105	82-83	-0.4322833170236322
1105	84-85	-0.49186873783212093
1105	86-87	-0.4135083406017088
1105	88-89	-0.4540403771593873
1105	90-91	-0.3288004645661502
1105	92-93	-0.4195382902583802
1105	94-95	-0.3768440486783078
1105	96-97	-0.30902378112681816
1105	98-99	-0.2483484154789224
1105	100	-0.22224545421811115
1106	1	-0.04186853311159666
1106	2	-0.04462512835929999
1106	3	-0.039071444855593995
1106	4	-0.03157300169853272
1106	5	-0.11041197434411032
1106	6	-0.08273878938857138
1106	7	-0.08437548213861845
1106	8	-0.05840134865277946
1106	9	-0.04507252281875651
1106	10-11	-0.043446696298090615
1106	12-13	-0.060153599680951686
1106	14-15	-0.01584080328375137
1106	16-17	-0.056827388987386485
1106	18-19	-0.031180791403151886
1106	20-21	-0.02442063277248252
1106	22-23	0.05808441449768509
1106	24-25	-0.0523963878339444
1106	26-27	-0.005941717672762081
1106	28-29	-0.12176414695573357
1106	30-31	-0.0022552104425130892
1106	32-33	-0.10014494370798133
1106	34-35	-0.11614795686600843
1106	36-37	-0.16568376388089234
1106	38-39	-0.2314922937677153
1106	40-41	-0.3185855062405736
1106	42-43	-0.2024861535768565
1106	44-45	-0.19048546083582352
1106	46-47	-0.19986004631238075
1106	48-49	-0.3016735086711648
1106	50-51	-0.3713397978842963
1106	52-53	-0.31192479769758563
1106	54-55	-0.3240124393713799
1106	56-57	-0.326758700282646
1106	58-59	-0.35168398761351227
1106	60-61	-0.32156658032793217
1106	62-63	-0.35542323020239763
1106	64-65	-0.3416077713514767
1106	66-67	-0.28148354613082915
1106	68-69	-0.3425708373192933
1106	70-71	-0.41431955379439245
1106	72-73	-0.28493106708681637
1106	74-75	-0.3530184649998418
1106	76-77	-0.3176226156515014
1106	78-79	-0.30839493460393186
1106	80-81	-0.2679190396804785
1106	82-83	-0.17942737040969092
1106	84-85	-0.164172418315097
1106	86-87	-0.23541348280802765
1106	88-89	-0.2650490263649701
1106	90-91	-0.31955174098558103
1106	92-93	-0.255700086263694
1106	94-95	-0.38057709808532536
1106	96-97	-0.3285155375733595
1106	98-99	-0.3704245434036082
1106	100	-0.24850622499696584
1107	1	0.03734151140432118
1107	2	0.05206156267129103
1107	3	0.05954819291897451
1107	4	0.05233752116143364
1107	5	-0.07945398154550531
1107	6	-0.16939273519515297
1107	7	-0.1557753865549003
1107	8	-0.03329998594659145
1107	9	-0.0848120926352891
1107	10-11	-0.08876405126680709
1107	12-13	0.007161837940742544
1107	14-15	0.02775881012690462
1107	16-17	-0.005327405296185361
1107	18-19	0.011304860402653105
1107	20-21	0.02122697529456019
1107	22-23	0.03383252728647079
1107	24-25	0.05722388669270373
1107	26-27	0.04201464850081038
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2216	9	-0.15288547439848088
2216	10-11	-0.15609208220067217
2216	12-13	-0.13663344541220823
2216	14-15	-0.19586917008002303
2216	16-17	-0.1943246870986144
2216	18-19	-0.11730046018194429
2216	20-21	-0.14816865644709765
2216	22-23	-0.2082934334145179
2216	24-25	-0.2835593657646811
2216	26-27	-0.22745121132059154
2216	28-29	-0.3841697629985177
2216	30-31	-0.22936034609554667
2216	32-33	-0.20210709176419783
2216	34-35	-0.23164960350023733
2216	36-37	-0.3245179383415433
2216	38-39	-0.25829244993082057
2216	40-41	-0.23786172184765775
2216	42-43	-0.2739878451413773
2216	44-45	-0.2632391287257292
2216	46-47	-0.20753826775311524
2216	48-49	-0.1846536434134478
2216	50-51	-0.14758348639355034
2216	52-53	-0.0984740667911268
2216	54-55	0.04452336763726095
2216	56-57	0.016224980309175407
2216	58-59	-0.05301553304532547
2216	60-61	-0.027935782196060188
2216	62-63	-0.0218120678194893
2216	64-65	7.171341127687469E-4
2216	66-67	-0.05981558215277971
2216	68-69	0.06118985791007958
2216	70-71	-0.02617532029291425
2216	72-73	-0.03519457713784391
2216	74-75	-0.04977380016762112
2216	76-77	-0.023318925782191968
2216	78-79	0.006996389965159722
2216	80-81	0.023686100829884538
2216	82-83	0.04633334789198784
2216	84-85	-0.029467173129319235
2216	86-87	-0.004245343966161386
2216	88-89	-2.4495276674230126E-4
2216	90-91	-0.04088439753119388
2216	92-93	0.07518505249803553
2216	94-95	0.07661572448376575
2216	96-97	0.0228745083041062
2216	98-99	-0.03705230084806743
2216	100	-0.05922768394404443
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	7.0
3	1.0
4	5.0
5	15.0
6	27.0
7	57.0
8	141.0
9	293.0
10	444.0
11	824.0
12	1272.0
13	2067.0
14	2354.0
15	2909.0
16	3722.0
17	5502.0
18	6455.0
19	3462.0
20	2219.0
21	2089.0
22	2119.0
23	2362.0
24	2648.0
25	3009.0
26	3988.0
27	5028.0
28	6259.0
29	8278.0
30	10476.0
31	13359.0
32	17212.0
33	22483.0
34	31337.0
35	47580.0
36	86970.0
37	204635.0
38	391285.0
39	208309.0
40	1067.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.634687771012395	19.877859961223944	13.180862608348203	23.30658965941545
2	15.912942499954639	20.20839003501896	40.443452542957196	23.435214922069203
3	18.309958821304058	25.42428390891878	30.336787118207987	25.928970151569175
4	11.969978208526797	16.934297166019817	39.478855376107035	31.616869249346347
5	11.756964883572977	37.8446592954207	35.995494845482455	14.402880975523868
6	30.071842847513352	38.850205984575425	17.665955707646148	13.411995460265072
7	26.542227012550114	32.82445172066219	22.700347752401743	17.932973514385953
8	24.89292502256717	36.20309090826616	20.596771193859915	18.307212875306757
9	24.666731986652948	16.216503094534353	21.9156718977089	37.2010930211038
10-11	23.590604057289415	26.754912393866075	29.094190769353595	20.560292779490922
12-13	24.71656192816817	24.041998822429008	29.55898242624985	21.682456823152968
14-15	21.66123771113844	26.13492084732531	27.203625976792406	25.000215464743846
16-17	20.438159832128093	29.829061690023035	27.538060128698167	22.19471834915071
18-19	20.178930995487942	28.071835491987663	30.656641281139756	21.09259223138464
20-21	21.627978288421428	27.40605968234614	30.18482784147971	20.781134187752716
22-23	20.960021047522492	27.432224936324438	30.485794056342947	21.12195995981012
24-25	20.928277812907396	27.40361455073952	30.429288003723236	21.238819632629845
26-27	20.692347157021022	27.205431070341994	30.596357180150974	21.50586459248601
28-29	20.769093625258527	27.382912286798266	30.56499791998309	21.282996167960118
30-31	21.107251990492465	28.0214509426308	30.002187204761388	20.869109862115344
32-33	20.53742427824136	27.529393722291317	30.767070319430008	21.166111680037314
34-35	20.698947540366216	27.169021977099533	30.706230134512463	21.425800348021788
36-37	21.347463733264124	27.740944886176987	29.93836515199088	20.973226228568006
38-39	21.11303692881482	27.871885733471874	30.004673384846438	21.01040395286687
40-41	20.689950894757235	27.61441630464671	30.352457848535146	21.343174952060906
42-43	20.742973372613267	27.65188345419642	30.027917308867615	21.577225864322696
44-45	21.129161420582797	27.820963918285862	29.78623200735019	21.26364265378115
46-47	21.09705273072243	28.420718810312586	29.325742170876467	21.156486288088516
48-49	21.049699469529354	28.022804839155295	29.390832062692894	21.53666362862246
50-51	21.060904784501023	27.931485633250254	29.309866145025737	21.69774343722299
52-53	21.1576418926735	28.01952463147485	29.180115178994694	21.642718296856962
54-55	21.31256486931781	28.56377515840815	28.915793274639455	21.207866697634582
56-57	20.97117264974014	28.17399564248805	28.987071262966275	21.86776044480553
58-59	20.876209277397926	27.615774688265333	29.55774025989343	21.950275774443316
60-61	21.347646929677335	28.174245324614294	28.969972656228737	21.508135089479634
62-63	21.03326690868645	28.171020860484198	29.079434230754053	21.716278000075302
64-65	21.01531519026273	27.823638974850283	29.383917643995538	21.77712819089145
66-67	20.930296907901123	27.873525417301348	29.01331898706386	22.182858687733667
68-69	21.50937644731512	28.18409326685758	28.85430931911039	21.45222096671691
70-71	21.58180798792304	27.842649310872897	28.967742754911708	21.607799946292356
72-73	21.17739649130765	27.996960796543473	29.26553339154693	21.560109320601946
74-75	21.15144026425635	28.019447264400597	29.079357648764404	21.74975482257864
76-77	21.329761899361767	28.162964441400206	28.647240001270113	21.86003365796791
78-79	21.47363436115355	28.336484412139335	28.79313379323359	21.39674743347353
80-81	21.348424696805846	27.997058780836532	28.924377463112283	21.730139059245335
82-83	21.042907577990583	28.079467314786967	29.136563864662246	21.741061242560203
84-85	21.09564347246648	28.106839958249623	28.938899022059566	21.85861754722433
86-87	21.35205052498647	28.207062448146615	28.71451686139423	21.726370165472687
88-89	20.943535989643177	28.103633552079188	28.821425995571857	22.131404462705774
90-91	20.945522372488025	27.916915018021914	28.877660534769646	22.259902074720415
92-93	21.11598892829246	28.32720506518826	28.630624647885405	21.926181358633873
94-95	20.884058247124795	27.92299338909105	28.945157670223875	22.247790693560283
96-97	20.80776108191376	27.90008609513649	29.002085697774316	22.290067125175433
98-99	20.830033322174533	28.038754605273304	28.850353225936683	22.28085884661548
100	21.075436213846167	27.93945942415145	28.662604137465536	22.322500224536842
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	54.0
1	45.0
2	138.5
3	248.5
4	156.0
5	85.0
6	210.0
7	444.5
8	736.0
9	856.5
10	659.0
11	472.5
12	488.0
13	569.0
14	667.5
15	772.5
16	868.0
17	980.0
18	1112.5
19	1302.0
20	1614.0
21	2051.5
22	2759.5
23	3735.0
24	5075.0
25	6628.0
26	8613.0
27	11193.0
28	14344.0
29	17532.5
30	20440.0
31	23688.0
32	26878.5
33	29780.5
34	32390.0
35	34494.0
36	36499.5
37	38448.0
38	39293.5
39	39344.0
40	39655.0
41	39928.0
42	39201.0
43	38068.5
44	37247.5
45	36246.0
46	35547.0
47	35115.5
48	35056.0
49	34695.5
50	33108.5
51	31084.5
52	28602.5
53	26696.0
54	25694.5
55	24558.5
56	22924.0
57	21058.5
58	19279.5
59	17458.0
60	15411.0
61	13068.0
62	10787.5
63	8721.0
64	7042.5
65	5539.5
66	4177.0
67	3064.5
68	2193.0
69	1591.5
70	1123.5
71	777.5
72	557.5
73	407.5
74	320.5
75	225.5
76	144.0
77	106.5
78	64.5
79	38.5
80	23.0
81	13.5
82	4.0
83	1.5
84	1.0
85	0.0
86	0.0
87	0.5
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09743538101860798
2	8.164976063011842E-4
3	0.0
4	2.7216586876706143E-4
5	0.0381032216273886
6	1.8144391251137426E-4
7	0.004354653900272983
8	3.628878250227485E-4
9	2.7216586876706143E-4
10-11	5.443317375341229E-4
12-13	0.0
14-15	4.5360978127843565E-5
16-17	0.0
18-19	4.5360978127843565E-5
20-21	0.0
22-23	1.3608293438353072E-4
24-25	1.8144391251137426E-4
26-27	0.001678356190730212
28-29	0.010387663991276178
30-31	0.03683311423980897
32-33	0.026354728292277113
34-35	0.02962071871748185
36-37	0.026762977095427704
38-39	0.025765035576615147
40-41	0.031752684689490496
42-43	0.023995957429629247
44-45	0.02327018177958375
46-47	0.017962947338626054
48-49	0.01220210311638992
50-51	0.009616527363102836
52-53	0.0064866198722816305
54-55	0.004808263681551418
56-57	0.003129907490821206
58-59	8.618585844290278E-4
60-61	6.350536937898099E-4
62-63	0.001587634234474525
64-65	0.002721658687670614
66-67	7.25775650045497E-4
68-69	0.0011340244531960891
70-71	4.5360978127843566E-4
72-73	5.896927156619664E-4
74-75	5.443317375341229E-4
76-77	3.628878250227485E-4
78-79	9.072195625568713E-5
80-81	4.5360978127843566E-4
82-83	4.082488031505921E-4
84-85	3.1752684689490495E-4
86-87	2.2680489063921783E-4
88-89	4.5360978127843565E-5
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1102269.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	78.8577753293805
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.50279821137595	68.21418226715423
2	9.619545934162693	15.171519840937147
3	2.0402839722605486	4.8267676527797505
4	0.7198079023188166	2.270497993654797
5	0.3485784413563198	1.3744060206571156
6	0.1952188057358378	0.9236712433672007
7	0.12870296727949607	0.710446077456578
8	0.09103648909860103	0.5743148003290464
9	0.0614720360086131	0.43627932041461204
>10	0.2767682609735328	3.6826216482386673
>50	0.010953662736055199	0.5890088810990709
>100	0.004257924064133089	0.5859637877274879
>500	2.3015705175518172E-4	0.1253234926020897
>1k	3.4523557763277256E-4	0.5149969735820847
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2374	0.21537392415100126	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2165	0.19641303529356266	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1136	0.10306014230646059	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	4.5360978127843565E-5	0.0
16-17	0.0	0.0	0.0	1.8144391251137426E-4	0.0
18-19	0.0	0.0	0.0	2.721658687670614E-4	0.0
20-21	0.0	0.0	0.0	2.721658687670614E-4	0.0
22-23	0.0	0.0	0.0	4.082488031505921E-4	0.0
24-25	0.0	0.0	0.0	6.804146719176535E-4	0.0
26-27	0.0	0.0	0.0	9.525805406847148E-4	0.0
28-29	0.0	0.0	0.0	0.0014969122782188377	0.0
30-31	0.0	0.0	0.0	0.005579400309724759	0.0
32-33	0.0	0.0	0.0	0.013835098328992289	0.0
34-35	0.0	0.0	0.0	0.022725850042049626	0.0
36-37	0.0	0.0	0.0	0.04050735346816431	0.0
38-39	0.0	0.0	0.0	0.06863115990742731	0.0
40-41	0.0	0.0	0.0	0.11172408912887871	0.0
42-43	0.0	0.0	0.0	0.15685826236608305	0.0
44-45	0.0	0.0	0.0	0.2026274892970772	0.0
46-47	0.0	0.0	0.0	0.2544750872972024	0.0
48-49	0.0	0.0	0.0	0.31838870547933396	0.0
50-51	0.0	0.0	0.0	0.39318895841214807	0.0
52-53	0.0	0.0	0.0	0.4640881672259675	0.0
54-55	0.0	0.0	0.0	0.5388884201587816	0.0
56-57	0.0	0.0	0.0	0.62285159067342	0.0
58-59	0.0	0.0	0.0	0.7232354352703378	0.0
60-61	0.0	0.0	0.0	0.8300605387614095	0.0
62-63	0.0	0.0	0.0	0.9387000813775948	0.0
64-65	0.0	0.0	0.0	1.0538262438660617	0.0
66-67	0.0	0.0	0.0	1.1873689634744333	0.0
68-69	0.0	0.0	0.0	1.3302560445771405	0.0
70-71	0.0	0.0	0.0	1.4927390682310762	0.0
72-73	0.0	0.0	0.0	1.6752716442175186	0.0
74-75	0.0	0.0	0.0	1.8507732685941454	0.0
76-77	0.0	0.0	0.0	2.036163586202642	0.0
78-79	0.0	0.0	0.0	2.248044715037799	0.0
80-81	0.0	0.0	0.0	2.4727176397050084	0.0
82-83	0.0	0.0	0.0	2.7154442336670996	0.0
84-85	0.0	0.0	0.0	2.9607110424043492	0.0
86-87	0.0	0.0	0.0	3.2046170217977643	0.0
88	0.0	0.0	0.0	3.4090589502199555	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3905	0.0	33.26209	1
GGTATCA	2525	0.0	32.616558	1
GTCTCGC	285	0.0	28.071491	1
ATCAACG	4590	0.0	27.549143	3
CAACGCA	4635	0.0	27.484514	5
TCAACGC	4620	0.0	27.472002	4
CAGCGCG	70	0.008842546	26.852982	9
AACGCAG	4830	0.0	26.370102	6
TATCAAC	4860	0.0	25.921913	2
GTACATG	3050	0.0	24.379158	1
ACGCAGA	5225	0.0	24.196674	7
CGCAGAG	5240	0.0	24.034443	8
TATACTG	630	0.0	23.876898	5
TACATGG	3055	0.0	23.080677	2
GAGTACT	3375	0.0	22.695744	12-13
TCTGTCG	275	6.002665E-11	22.21474	8
ACACCGT	150	3.2916934E-5	21.93292	6
ACTCCGC	110	0.0030622156	21.360327	8
AGAGTAC	4720	0.0	21.3558	10-11
ACATGGG	3250	0.0	21.26192	3
>>END_MODULE