FastQCFastQC Report
Wed 25 May 2016
SRR1033132_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033132_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2146023
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACCG550.002724171434.1648065
TCAACGC27150.027.8578644
GTACATG47500.027.6686821
ATCAACG27500.027.5077953
TACATGG46500.026.585682
GTATCAA31350.026.1450581
CAACGCA29600.025.8688765
ACATGGG46600.025.2067433
AACGCAG31150.024.72446
GGACCGA1751.6607373E-724.1515166
GGTATCA20850.023.270681
TATCAAC34550.022.0400162
ACGGACC1503.3140983E-521.9152668
ACGCAGA34150.021.8648437
AGGACCG1954.700487E-721.681515
CGCAGAG34000.021.5469428
GTCTCGC4900.021.1497141
TAGGTCG1150.003971170620.4246125
GACCGAC1200.00508973219.5672027
TACGCGA600.005091917719.56628880-81
Produced by FastQC (version 0.11.5)