##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033132_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2146023
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.62654640700496	33.0	31.0	34.0	30.0	34.0
2	31.71110328267684	34.0	31.0	34.0	30.0	34.0
3	31.79584188985859	34.0	31.0	34.0	30.0	34.0
4	35.16086267481756	37.0	35.0	37.0	33.0	37.0
5	35.2293810457763	37.0	35.0	37.0	33.0	37.0
6	35.33057893601327	37.0	36.0	37.0	33.0	37.0
7	35.228958403521304	37.0	35.0	37.0	33.0	37.0
8	35.25047821015898	37.0	36.0	37.0	33.0	37.0
9	36.900147388914284	39.0	38.0	39.0	34.0	39.0
10-11	36.90278855352436	39.0	38.0	39.0	34.0	39.0
12-13	36.87844188995179	39.0	38.0	39.0	34.0	39.0
14-15	38.33807349688237	41.0	39.0	41.0	34.0	41.0
16-17	38.36195138635513	41.0	39.0	41.0	34.0	41.0
18-19	38.35953272635009	41.0	39.0	41.0	34.0	41.0
20-21	38.383361222130425	41.0	39.0	41.0	34.0	41.0
22-23	38.35168425501497	41.0	39.0	41.0	34.0	41.0
24-25	38.35061693187818	41.0	39.0	41.0	34.0	41.0
26-27	38.30703585189907	40.5	39.0	41.0	34.0	41.0
28-29	38.28285530956565	40.0	39.0	41.0	34.0	41.0
30-31	38.29166113317518	40.0	39.0	41.0	34.0	41.0
32-33	38.352079870532606	41.0	39.0	41.0	34.0	41.0
34-35	38.32594757838103	41.0	39.0	41.0	34.0	41.0
36-37	38.25065831074504	41.0	39.0	41.0	33.5	41.0
38-39	38.174071526726415	41.0	39.0	41.0	33.0	41.0
40-41	38.11543352517657	41.0	38.5	41.0	33.0	41.0
42-43	38.01542154953604	40.5	38.0	41.0	33.0	41.0
44-45	37.914636981989474	40.0	38.0	41.0	33.0	41.0
46-47	37.80284437771636	40.0	38.0	41.0	33.0	41.0
48-49	37.69781777734907	40.0	38.0	41.0	32.5	41.0
50-51	37.58901325847859	40.0	38.0	41.0	32.0	41.0
52-53	37.45762929847443	40.0	38.0	41.0	32.0	41.0
54-55	37.31884886601868	40.0	37.0	41.0	31.5	41.0
56-57	37.18255442742226	40.0	37.0	41.0	31.0	41.0
58-59	37.005476642142234	40.0	36.5	41.0	31.0	41.0
60-61	36.801389826670075	40.0	36.0	41.0	31.0	41.0
62-63	36.55268978943842	39.0	35.5	41.0	31.0	41.0
64-65	36.29255161757353	39.0	35.0	41.0	30.5	41.0
66-67	36.01207186502661	39.0	35.0	41.0	30.0	41.0
68-69	35.707308122979114	38.0	35.0	40.0	30.0	41.0
70-71	35.363466281582255	37.0	35.0	40.0	29.5	41.0
72-73	34.99807620887567	37.0	35.0	39.5	29.0	41.0
74-75	34.625363288277896	36.5	34.0	39.0	29.0	41.0
76-77	34.288247609648174	36.0	34.0	39.0	29.0	40.5
78-79	33.90561214861164	35.5	34.0	38.5	28.5	39.5
80-81	33.53109822215326	35.0	34.0	37.0	28.0	39.0
82-83	33.285398851736446	35.0	34.0	37.0	28.0	39.0
84-85	32.99197771878493	35.0	34.0	36.5	28.0	38.5
86-87	32.717640258282415	35.0	34.0	36.0	28.0	37.0
88-89	32.33325900980558	35.0	34.0	36.0	27.0	37.0
90-91	32.12253876123415	35.0	34.0	35.0	26.0	36.5
92-93	31.928543403309284	35.0	34.0	35.0	26.0	36.0
94-95	31.749832364331603	35.0	33.5	35.0	26.0	36.0
96-97	31.564756295715377	35.0	33.0	35.0	25.0	36.0
98-99	31.36946388738611	35.0	33.0	35.0	25.0	36.0
100	31.208201869225075	35.0	33.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.1675029424407697
1101	2	0.19346711032650177
1101	3	0.1804274374258199
1101	4	0.17163434585019388
1101	5	0.19893937389262106
1101	6	0.1482363837127636
1101	7	0.16107017201949958
1101	8	0.17049349393836621
1101	9	0.1449258413501795
1101	10-11	0.16133392961717874
1101	12-13	0.2390405648375662
1101	14-15	0.2673342814476385
1101	16-17	0.23097319477989942
1101	18-19	0.1821821723407453
1101	20-21	0.1538384569222515
1101	22-23	0.13999602394171973
1101	24-25	0.14788856490758207
1101	26-27	0.18883010128939048
1101	28-29	0.21458911979042483
1101	30-31	0.23210644350605492
1101	32-33	0.28201600262559623
1101	34-35	0.32182681277507896
1101	36-37	0.2983832859904325
1101	38-39	0.30158908142369256
1101	40-41	0.28056463113296104
1101	42-43	0.22069222410769385
1101	44-45	0.15193377360577642
1101	46-47	0.15678731152022607
1101	48-49	0.19727858012038268
1101	50-51	0.2141054335116337
1101	52-53	0.12248576651172982
1101	54-55	0.09997682506678984
1101	56-57	0.05307120593938208
1101	58-59	0.03856502695168729
1101	60-61	0.1410528823895305
1101	62-63	0.11798503246166092
1101	64-65	0.08850083675935849
1101	66-67	0.17396186862702478
1101	68-69	0.14621053887407243
1101	70-71	0.15249051352016352
1101	72-73	0.2431591724960711
1101	74-75	0.06141216181286069
1101	76-77	0.0763013841233331
1101	78-79	0.16234528648157465
1101	80-81	0.14497038765971837
1101	82-83	0.09520391281219531
1101	84-85	0.038097537016071215
1101	86-87	0.10221858936214545
1101	88-89	0.25681337587564457
1101	90-91	0.19757784774988352
1101	92-93	0.206205303946561
1101	94-95	0.24775160330795387
1101	96-97	0.25640150401399
1101	98-99	0.3102113897051524
1101	100	0.4857393262307603
1102	1	0.06431369494182348
1102	2	0.11527942306905103
1102	3	0.1104368262074793
1102	4	0.005886930953963088
1102	5	-0.06495793625104795
1102	6	-0.07363906756145866
1102	7	-0.011136528773555199
1102	8	0.07717396969130164
1102	9	0.021483975948221712
1102	10-11	0.04165651694856365
1102	12-13	0.06547053186066165
1102	14-15	-0.029118093832124714
1102	16-17	0.02606924187960402
1102	18-19	0.013671080048709428
1102	20-21	0.03086324039033883
1102	22-23	0.08627286635914544
1102	24-25	0.12552700160483
1102	26-27	0.09270584365685153
1102	28-29	0.03820581196001882
1102	30-31	-0.019970329030783773
1102	32-33	-0.017754049666407923
1102	34-35	0.05817185255098423
1102	36-37	0.09498456752951512
1102	38-39	0.03042412084882784
1102	40-41	0.1384721855717146
1102	42-43	-0.019257030461304225
1102	44-45	-0.009358240736602852
1102	46-47	0.002853601056408195
1102	48-49	0.010855687710538575
1102	50-51	0.043932797915260835
1102	52-53	0.04595142274929742
1102	54-55	0.10720535557440769
1102	56-57	0.09164436167699108
1102	58-59	0.0983334939472229
1102	60-61	-0.010223520427011579
1102	62-63	0.13670536794319332
1102	64-65	0.10284680174708427
1102	66-67	0.03250280139177164
1102	68-69	-0.08576064289611907
1102	70-71	0.11447471704306622
1102	72-73	-0.03636742973528584
1102	74-75	-0.05697144622168793
1102	76-77	-0.025902861282382617
1102	78-79	0.04471634561905802
1102	80-81	0.07893726635219167
1102	82-83	0.038472564075675564
1102	84-85	0.004673804581315721
1102	86-87	0.005324346858252227
1102	88-89	0.11615208175440728
1102	90-91	0.2398129586044675
1102	92-93	0.25850277662867427
1102	94-95	0.23339322472002522
1102	96-97	0.29038897333873237
1102	98-99	0.3332973833831048
1102	100	0.279414129993377
1103	1	0.07143536820863616
1103	2	0.08790553198011253
1103	3	0.056547639495388324
1103	4	0.10861878631564537
1103	5	0.07514443807236404
1103	6	0.11137701489137442
1103	7	0.09432934356981093
1103	8	0.06481728509759677
1103	9	0.1331467805204838
1103	10-11	0.18078551378395247
1103	12-13	0.12714587382895104
1103	14-15	0.11200823988742314
1103	16-17	0.08391354471391566
1103	18-19	0.14873039490163364
1103	20-21	0.10513693606882413
1103	22-23	0.043030955141212246
1103	24-25	0.03971220056033786
1103	26-27	0.11680271881896687
1103	28-29	0.15141849807550756
1103	30-31	0.09990360529055664
1103	32-33	0.12416386929878342
1103	34-35	0.11821906233188884
1103	36-37	0.1553456445350676
1103	38-39	0.05725090591462845
1103	40-41	0.08559398853573441
1103	42-43	0.0871733567280728
1103	44-45	0.05582454585098162
1103	46-47	0.1716123556174196
1103	48-49	0.11630513174186774
1103	50-51	0.04406703451753913
1103	52-53	0.07272330266698646
1103	54-55	0.1807335085133417
1103	56-57	0.09451412839496243
1103	58-59	0.11324629419624443
1103	60-61	0.11687584492135272
1103	62-63	0.15095174074481577
1103	64-65	0.17479416197672748
1103	66-67	0.19887145271381712
1103	68-69	0.1779481350103964
1103	70-71	0.20227280444169082
1103	72-73	0.2407057800110337
1103	74-75	0.22840541060990205
1103	76-77	0.16052302973738364
1103	78-79	0.12492303972388896
1103	80-81	0.17395840699584397
1103	82-83	0.10159683993296653
1103	84-85	0.095238117099683
1103	86-87	0.08878449523184173
1103	88-89	0.14707189429871903
1103	90-91	0.158621862749591
1103	92-93	0.11481903028180795
1103	94-95	0.12823776100930573
1103	96-97	0.0724767091714753
1103	98-99	0.09587757948526843
1103	100	0.10102816841833473
1104	1	-0.0035956523898725834
1104	2	-0.008258115209503814
1104	3	-0.0309189977468165
1104	4	-0.0035896929255372356
1104	5	-0.07662977360886458
1104	6	-0.08422066251938531
1104	7	-0.03277381137079516
1104	8	-0.004400496159298939
1104	9	-0.044263457526177774
1104	10-11	-0.03583335548400157
1104	12-13	-0.03040025109454092
1104	14-15	-0.10112393492718752
1104	16-17	0.016073005163697474
1104	18-19	-0.01195703405286963
1104	20-21	-0.05972651084014302
1104	22-23	-0.09567556917769338
1104	24-25	-0.009684615352192338
1104	26-27	-0.03485438489808956
1104	28-29	-0.07803660116959321
1104	30-31	-0.0527496968417438
1104	32-33	-0.03785864249529425
1104	34-35	-0.0946026462576981
1104	36-37	-0.04328767949902357
1104	38-39	-0.02666423713404953
1104	40-41	-0.04647220192657642
1104	42-43	-0.07330546390625159
1104	44-45	-0.14563571239030182
1104	46-47	-0.0746326425713093
1104	48-49	-0.13889206766020123
1104	50-51	-0.13434403421494778
1104	52-53	-0.13867421693429094
1104	54-55	-0.16299902938799704
1104	56-57	-0.18410414336214131
1104	58-59	-0.11431431047641638
1104	60-61	-0.0401627080614162
1104	62-63	-0.05218966738215869
1104	64-65	-0.011063463885705005
1104	66-67	-0.0368164967205189
1104	68-69	-0.058130122256542904
1104	70-71	-0.04531383463260141
1104	72-73	-0.02631937582063415
1104	74-75	-0.07120938225994422
1104	76-77	-0.22131784500185603
1104	78-79	-0.06731259815538237
1104	80-81	0.012204879183478567
1104	82-83	0.01307482571762364
1104	84-85	0.04599473864422521
1104	86-87	0.016892176265145054
1104	88-89	0.21942952224307533
1104	90-91	0.09473488806990105
1104	92-93	0.07125153653797867
1104	94-95	0.09598175154906485
1104	96-97	0.1034214737688508
1104	98-99	0.12870205702760273
1104	100	0.1328366693114802
1105	1	0.08925202991843051
1105	2	0.13421431225836145
1105	3	0.08756528597104918
1105	4	0.062176170407383324
1105	5	0.03178499852972294
1105	6	0.08636049197408369
1105	7	0.0205231243437467
1105	8	0.030099331295673437
1105	9	0.046682354361401224
1105	10-11	0.09624848592630997
1105	12-13	0.05543641280650746
1105	14-15	0.13147884561757195
1105	16-17	0.14338737241293842
1105	18-19	0.1553259304093757
1105	20-21	0.15040931636028887
1105	22-23	0.19227296631070345
1105	24-25	0.13904756621248993
1105	26-27	0.19884189465585678
1105	28-29	0.18566844024529416
1105	30-31	0.23610490399185835
1105	32-33	0.26154573044019
1105	34-35	0.2019225090124337
1105	36-37	0.23747693563582573
1105	38-39	0.15327177435916184
1105	40-41	0.12202867344369395
1105	42-43	0.19529023984495097
1105	44-45	0.2143545019000186
1105	46-47	0.26659228027292414
1105	48-49	0.2172630478715405
1105	50-51	0.22835208788656303
1105	52-53	0.23323394342571646
1105	54-55	0.16701576950934793
1105	56-57	0.23986086181421484
1105	58-59	0.29461546573717357
1105	60-61	0.19123475573064042
1105	62-63	0.20255776830504857
1105	64-65	0.1746950244730172
1105	66-67	0.21786908993665577
1105	68-69	0.1385731969713362
1105	70-71	0.09345948036119012
1105	72-73	0.11062085221617934
1105	74-75	0.12945089820119193
1105	76-77	0.1931896633582184
1105	78-79	0.2353180200616123
1105	80-81	0.12822545038502398
1105	82-83	0.21580778801796896
1105	84-85	0.1701323704290587
1105	86-87	0.14612727967386974
1105	88-89	0.16519546919710137
1105	90-91	0.10196643166668196
1105	92-93	0.1497307152889107
1105	94-95	0.21461461570677187
1105	96-97	0.24392717346545112
1105	98-99	0.16330182190951348
1105	100	0.22391984417260602
1106	1	0.25560300405537717
1106	2	0.284310681556903
1106	3	0.22293566689087996
1106	4	0.1934188121604521
1106	5	0.22402988098765775
1106	6	0.22766731662943585
1106	7	0.17335628677585646
1106	8	0.12515601353334205
1106	9	0.1847111986893779
1106	10-11	0.14365339838691682
1106	12-13	0.17640243194744443
1106	14-15	0.23485746777395633
1106	16-17	0.2422335518212364
1106	18-19	0.27345902448100645
1106	20-21	0.1270670581855171
1106	22-23	0.1207745423997224
1106	24-25	0.14581359294903962
1106	26-27	0.23548003031344678
1106	28-29	0.1889298051763646
1106	30-31	0.14033375971325057
1106	32-33	0.10963828294484301
1106	34-35	0.19579142777738667
1106	36-37	0.13182081737218
1106	38-39	0.18522911551597332
1106	40-41	0.20582723787345714
1106	42-43	0.21736735427280962
1106	44-45	0.23081323875011606
1106	46-47	0.3055126592938464
1106	48-49	0.28299118201000795
1106	50-51	0.21647326634541741
1106	52-53	0.2371539901387152
1106	54-55	0.2631073535737585
1106	56-57	0.18775853945653154
1106	58-59	0.16802394813434773
1106	60-61	0.22303781106466403
1106	62-63	0.21625953618321603
1106	64-65	0.17132328583794276
1106	66-67	0.2713172593947064
1106	68-69	0.23020592075698687
1106	70-71	0.23617149281798788
1106	72-73	0.32711827920338266
1106	74-75	0.31786355818371703
1106	76-77	0.27358919243041413
1106	78-79	0.2230997697103163
1106	80-81	0.1960613662256634
1106	82-83	0.12798236361327042
1106	84-85	0.1855656999645845
1106	86-87	0.19166296005061412
1106	88-89	0.3249002848136797
1106	90-91	0.3533466963736842
1106	92-93	0.32313136471354653
1106	94-95	0.2517868176740379
1106	96-97	0.2824108049264673
1106	98-99	0.3302794338498103
1106	100	0.3615736552697939
1107	1	0.12383137998436666
1107	2	0.03092163148579985
1107	3	0.03904574808767336
1107	4	-0.016520962254524818
1107	5	-0.0036316153339299717
1107	6	-0.007533828264868703
1107	7	-0.020449998729908714
1107	8	0.03945928963698009
1107	9	-0.027431284638019804
1107	10-11	0.012901567817458215
1107	12-13	-0.01103566995174532
1107	14-15	-0.03380356602475132
1107	16-17	-0.032473739146198
1107	18-19	-0.009905694333610882
1107	20-21	0.013909539861053588
1107	22-23	-9.858264472626388E-4
1107	24-25	-0.014917191327583623
1107	26-27	0.053220157402122936
1107	28-29	0.04062561604055048
1107	30-31	0.08742923239512379
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2216	24-25	-0.20284599261261604
2216	26-27	-0.19493704415030066
2216	28-29	-0.10827149317186269
2216	30-31	-0.1145814087846162
2216	32-33	-0.11271161860129553
2216	34-35	-0.09649009008218457
2216	36-37	-0.17567238115508843
2216	38-39	-0.09587135888796183
2216	40-41	-0.08034023661980427
2216	42-43	-0.11507926314750705
2216	44-45	-0.0806408102865106
2216	46-47	-0.0026078913487239674
2216	48-49	0.02267148851806411
2216	50-51	0.008557075442006123
2216	52-53	0.015358377064359274
2216	54-55	0.07356788270017489
2216	56-57	0.021588147272261438
2216	58-59	-0.023626236733662154
2216	60-61	0.049791654066666524
2216	62-63	0.09441328830472884
2216	64-65	0.13258341279876618
2216	66-67	0.0815087152691163
2216	68-69	-0.022141461185753997
2216	70-71	6.512020004123542E-4
2216	72-73	0.030448937582058022
2216	74-75	0.12277622649710906
2216	76-77	0.06354505325121096
2216	78-79	0.012445512697183858
2216	80-81	0.07852308184740053
2216	82-83	-0.011201814054210502
2216	84-85	0.015144144587679875
2216	86-87	0.017258343787666774
2216	88-89	-0.016262266986259988
2216	90-91	-0.05733542677327108
2216	92-93	-0.0333677556098948
2216	94-95	-0.08217313956552985
2216	96-97	-0.11100535976141401
2216	98-99	-0.20288345001346642
2216	100	-0.1622492190393814
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	24534.0
3	4068.0
4	2486.0
5	2028.0
6	1746.0
7	1868.0
8	2151.0
9	2490.0
10	2827.0
11	3035.0
12	3477.0
13	3998.0
14	4564.0
15	4930.0
16	4872.0
17	4721.0
18	4664.0
19	4611.0
20	4623.0
21	4869.0
22	5622.0
23	7336.0
24	10973.0
25	15046.0
26	15757.0
27	13574.0
28	14571.0
29	18031.0
30	21831.0
31	26730.0
32	35112.0
33	47871.0
34	65717.0
35	97919.0
36	178936.0
37	399339.0
38	718455.0
39	359326.0
40	1315.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.866881176869924	20.195518476023764	13.31940980706238	23.618190540043933
2	15.051922404941106	19.90732857255491	41.61662748596096	23.424121536543023
3	18.103104993448692	25.446398179622403	30.086122884813555	26.36437394211535
4	11.658123594541882	16.66090187991095	40.300940514859164	31.380034010688007
5	12.071645208719675	38.61171791307364	36.544939551336384	12.77169732687031
6	30.608031719017525	39.14300307291963	17.85182692854367	12.397138279519183
7	26.71389500006291	32.8136104091222	22.964769347127785	17.50772524368711
8	24.314993319224556	37.69765611273477	20.488705598009798	17.498644970030885
9	24.141701259607142	17.235883074229996	21.722968454127827	36.899447212035035
10-11	23.197319542030947	27.516640423079103	29.592816193672316	19.693223841217634
12-13	23.88461963281964	24.723901676979526	29.604609771249795	21.78686891895104
14-15	21.0315068062205	27.06705085527795	26.472754727885174	25.428687610616375
16-17	20.72795182222152	30.454040094862293	27.035450785815772	21.782557297100418
18-19	20.53712204149906	29.448779594330638	29.109882883374137	20.904215480796164
20-21	21.811950331743923	28.84371902754322	28.509330101225533	20.835000539487325
22-23	21.24242701752595	28.664072179590516	28.653586108239093	21.43991469464444
24-25	21.239412593377168	28.688449137917978	28.63687685924124	21.435261409463617
26-27	21.08840557150008	28.522035911064048	28.71726958092573	21.672288936510142
28-29	20.986601654146693	28.69272449499869	28.806396978427863	21.514276872426755
30-31	21.331017299412476	29.24380436069354	28.3584433791227	21.06673496077128
32-33	20.84201126108963	28.815241179391847	28.97247509297821	21.37027246654031
34-35	20.97548994820599	28.506884546221617	29.067747458127524	21.449878047444866
36-37	21.43853095401868	29.04116732561976	28.35443940520862	21.16586231515294
38-39	21.21889145675201	29.165425843515074	28.535320700938087	21.080361998794828
40-41	20.905166588530477	28.858257845696716	28.900223037655433	21.336352528117377
42-43	20.93374122114228	28.642802169674837	28.89235345502706	21.53110315415582
44-45	21.154287527766005	28.841227037779475	28.748023204958255	21.256462229496265
46-47	21.16781535061792	29.09675205040027	28.58984608279705	21.145586516184757
48-49	21.082094812085803	28.809153286940287	28.724994343969158	21.383757557004753
50-51	21.061711382881974	28.658275876450944	28.79810166856604	21.48191107210104
52-53	21.13786509384657	28.670196393583115	28.74224178124532	21.449696731324995
54-55	21.261992324166947	29.205841126285907	28.466124188939567	21.066042360607582
56-57	21.075164645901225	28.876535495153938	28.55267247569746	21.49562738324738
58-59	20.92559269941665	28.42638896105273	29.063025477343412	21.584992862187207
60-61	21.21097740979821	28.96916404254055	28.543385745956634	21.276472801704603
62-63	20.995161380890217	28.961047100754513	28.57143189993679	21.472359618418487
64-65	21.012154370713198	28.70184535994561	28.758532826773077	21.527467442568117
66-67	20.916900437096338	28.695887193729668	28.598915180942974	21.788297188231017
68-69	21.465221777716682	28.86414207194466	28.461990116288554	21.20864603405011
70-71	21.297295584471744	28.6932448326446	28.634576779193765	21.37488280368989
72-73	21.056945650836727	28.79794555018323	28.804376190600458	21.34073260837959
74-75	21.17171283189253	28.851295416826755	28.596771762133617	21.380219989147093
76-77	21.165005925172732	28.88397053210987	28.41093531053451	21.540088232182892
78-79	21.31124011970523	29.00225915194195	28.5055066828585	21.180994045494327
80-81	21.210506461864085	28.682419074376153	28.6552281486428	21.451846315116967
82-83	21.066477599184704	28.785128037610946	28.844075712113536	21.30431865109081
84-85	21.083220368759243	28.76861702003725	28.6173322528499	21.530830358353608
86-87	21.327361502802468	28.884757619419528	28.483119940502267	21.30476093727574
88-89	21.01161058276068	28.713970673035	28.617559611711656	21.656859132492663
90-91	21.075669714860133	28.627637787596676	28.703123710644174	21.59356878689902
92-93	21.252828568086414	28.93085107754584	28.473615229411436	21.34270512495631
94-95	21.023088579468148	28.626346130632534	28.695491158226087	21.655074131673228
96-97	21.019764413367913	28.6439611705828	28.71397243988922	21.62230197616007
98-99	21.0614479049624	28.74033833918576	28.583328294199184	21.61488546165266
100	21.24200577613876	28.712734834354386	28.37142606687702	21.673833322629836
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	298.0
1	278.0
2	235.0
3	197.5
4	161.0
5	129.0
6	115.5
7	120.0
8	142.5
9	165.5
10	156.0
11	130.0
12	140.0
13	185.5
14	226.5
15	302.5
16	414.5
17	570.0
18	807.0
19	1191.5
20	1880.5
21	2859.5
22	4295.5
23	6463.5
24	9314.5
25	12825.0
26	17472.0
27	23126.0
28	29578.0
29	36815.0
30	43940.5
31	51092.5
32	57495.0
33	63297.5
34	68844.5
35	72547.0
36	75665.0
37	78666.5
38	80116.5
39	80737.5
40	81100.0
41	79963.5
42	77592.0
43	75229.0
44	72871.5
45	70951.5
46	69213.0
47	66992.0
48	65551.0
49	64222.5
50	61690.0
51	58329.0
52	54104.5
53	50664.5
54	48931.5
55	46604.5
56	42919.5
57	39185.5
58	35110.5
59	30802.5
60	26498.0
61	22152.5
62	18448.0
63	15013.0
64	11764.5
65	9174.5
66	7188.0
67	5407.5
68	4058.5
69	3141.0
70	2270.5
71	1601.0
72	1219.0
73	934.0
74	675.5
75	497.5
76	342.5
77	223.5
78	158.0
79	108.0
80	74.5
81	58.5
82	37.0
83	19.0
84	14.5
85	9.0
86	8.5
87	10.5
88	7.0
89	6.0
90	4.0
91	2.0
92	3.5
93	3.5
94	1.5
95	2.0
96	2.5
97	1.5
98	1.5
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3353645324397735
2	0.11817207923680222
3	0.06584272395962205
4	0.03797722577996601
5	0.041798247269484064
6	0.008434205970765458
7	0.006337303933834819
8	0.014631716435471568
9	0.01649562935718769
10-11	0.019547786766497845
12-13	0.014911303373728986
14-15	0.017497482552610105
16-17	0.01801005860608204
18-19	0.017893564048474784
20-21	0.02178448227255719
22-23	0.014888004462207534
24-25	0.02285623220254396
26-27	0.0181265531636893
28-29	0.017940161871517687
30-31	0.018685727040204134
32-33	0.022996025671672672
34-35	0.01451522187786431
36-37	0.009086575493366101
38-39	0.012581412221583833
40-41	0.009179771139451908
42-43	0.007991526651857879
44-45	0.008783689643587231
46-47	0.007525548421428848
48-49	0.0038909182240824073
50-51	0.0064537984914420764
52-53	0.007152765837085622
54-55	0.0031220541438745065
56-57	0.0025162824443167664
58-59	0.0026793748249669272
60-61	0.0032851465245246673
62-63	0.002772570471052733
64-65	0.0021434998599735417
66-67	0.0020037063908448327
68-69	0.0031686519669174096
70-71	0.0023764889751880574
72-73	0.002190097683016445
74-75	0.0024929835327953146
76-77	0.00438019536603289
78-79	0.003960814958646762
80-81	0.004030711693211117
82-83	0.00242308679823096
84-85	0.0032618476130032155
86-87	0.0027492715595312817
88-89	0.60432716704341
90-91	0.613926318590248
92-93	0.6103149873044231
94-95	0.5976403794367534
96-97	0.596219145833945
98-99	0.5927243091057273
100	0.594774613319615
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2146023.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.7818040073683
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.4843145632187	60.120597267781484
2	12.39376697593912	18.288689718634675
3	3.25815751600493	7.2117821781302895
4	1.217399929891004	3.5928785210320786
5	0.5664531965617858	2.0896969364034472
6	0.3076833746838462	1.3620860668349521
7	0.19657651150118385	1.015263875082277
8	0.11492847668807564	0.6783704273491994
9	0.09472107798137966	0.6289822809889921
>10	0.3532415496864373	4.264082792290624
>50	0.01085602399190592	0.526421321683665
>100	0.0018375997116449358	0.19624650920659314
>500	6.320413992946736E-5	0.024902104581659493
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	4.659782304290308E-5	0.0
9	0.0	0.0	0.0	1.3979346912870925E-4	0.0
10-11	4.659782304290308E-5	0.0	0.0	1.3979346912870925E-4	0.0
12-13	4.659782304290308E-5	0.0	0.0	1.3979346912870925E-4	0.0
14-15	4.659782304290308E-5	0.0	0.0	1.3979346912870925E-4	0.0
16-17	4.659782304290308E-5	0.0	0.0	1.3979346912870925E-4	0.0
18-19	4.659782304290308E-5	0.0	0.0	1.3979346912870925E-4	0.0
20-21	4.659782304290308E-5	0.0	0.0	1.630923806501608E-4	0.0
22-23	6.989673456435461E-5	0.0	0.0	2.0969020369306386E-4	0.0
24-25	9.319564608580616E-5	0.0	0.0	3.261847613003216E-4	0.0
26-27	9.319564608580616E-5	0.0	0.0	4.193804073861277E-4	0.0
28-29	9.319564608580616E-5	0.0	0.0	9.319564608580616E-4	0.0
30-31	1.164945576072577E-4	0.0	0.0	0.0035647334627820857	0.0
32-33	1.630923806501608E-4	0.0	0.0	0.009412760254666423	0.0
34-35	1.8639129217161231E-4	0.0	0.0	0.01630923806501608	0.0
36-37	1.8639129217161231E-4	0.0	0.0	0.030847758854401837	0.0
38-39	1.8639129217161231E-4	0.0	0.0	0.058014289688414336	0.0
40-41	1.8639129217161231E-4	0.0	0.0	0.09265977112081278	0.0
42-43	1.8639129217161231E-4	0.0	0.0	0.12700236670343235	0.0
44-45	1.8639129217161231E-4	0.0	0.0	0.16579505438664915	0.0
46-47	1.8639129217161231E-4	0.0	0.0	0.21388400776692515	0.0
48-49	1.8639129217161231E-4	0.0	0.0	0.26987129215297323	0.0
50-51	1.8639129217161231E-4	0.0	0.0	0.33047176102026865	0.0
52-53	1.8639129217161231E-4	0.0	0.0	0.3903499636303991	0.0
54-55	1.8639129217161231E-4	0.0	0.0	0.45602959520937103	0.0
56-57	1.8639129217161231E-4	0.0	0.0	0.5338712586025406	0.0
58-59	2.0969020369306386E-4	0.0	0.0	0.6212188778964625	0.0
60-61	2.329891152145154E-4	0.0	0.0	0.7158590564965985	0.0
62-63	2.329891152145154E-4	0.0	0.0	0.8121534578147578	0.0
64-65	2.329891152145154E-4	0.0	0.0	0.9148084619782733	0.0
66-67	2.329891152145154E-4	0.0	0.0	1.0376636224308873	0.0
68-69	2.329891152145154E-4	0.0	0.0	1.1703509235455538	0.0
70-71	2.329891152145154E-4	0.0	0.0	1.3181359193261208	0.0
72-73	2.329891152145154E-4	0.0	0.0	1.4774538763098066	0.0
74-75	2.329891152145154E-4	0.0	0.0	1.6326945237772383	0.0
76-77	2.56288026735967E-4	0.0	0.0	1.7976741162606364	0.0
78-79	3.0288584977887E-4	0.0	0.0	1.9864651963189583	0.0
80-81	3.261847613003216E-4	0.0	0.0	2.1950836500820357	0.0
82-83	3.261847613003216E-4	0.0	0.0	2.4150952715791023	0.0
84-85	3.261847613003216E-4	0.0	0.0	2.6336157627387964	0.0
86-87	3.261847613003216E-4	0.0	0.0	2.8646710682970316	0.0
88	3.261847613003216E-4	0.0	0.0	3.055745441684455	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGACCG	55	0.0027241714	34.164806	5
TCAACGC	2715	0.0	27.857864	4
GTACATG	4750	0.0	27.668682	1
ATCAACG	2750	0.0	27.507795	3
TACATGG	4650	0.0	26.58568	2
GTATCAA	3135	0.0	26.145058	1
CAACGCA	2960	0.0	25.868876	5
ACATGGG	4660	0.0	25.206743	3
AACGCAG	3115	0.0	24.7244	6
GGACCGA	175	1.6607373E-7	24.151516	6
GGTATCA	2085	0.0	23.27068	1
TATCAAC	3455	0.0	22.040016	2
ACGGACC	150	3.3140983E-5	21.915266	8
ACGCAGA	3415	0.0	21.864843	7
AGGACCG	195	4.700487E-7	21.68151	5
CGCAGAG	3400	0.0	21.546942	8
GTCTCGC	490	0.0	21.149714	1
TAGGTCG	115	0.0039711706	20.424612	5
GACCGAC	120	0.005089732	19.567202	7
TACGCGA	60	0.0050919177	19.566288	80-81
>>END_MODULE