FastQCFastQC Report
Wed 25 May 2016
SRR1033132_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033132_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2146023
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35220.16411753275710467No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31130.14505902313255728No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32250.041.4273641
GTATCAA55750.036.6221241
TCAACGC61350.032.25914
ATCAACG61500.032.2568633
CAACGCA63300.031.1910745
AACGCAG64800.030.458416
TATCAAC66900.029.8639722
ACGCAGA75250.025.9788427
CGCAGAG75800.025.665758
AGGACCG1851.002627E-825.4104885
GTACATG48200.024.40011
GCAGAGT85300.022.5318629
TACATGG48450.022.413152
ACATGGG48850.020.978613
TATAACG1805.658674E-620.893072
GAGTACT59600.020.06616412-13
TAGTACG1656.8518115E-519.9433844
AGAGTAC74000.019.81279210-11
GACAGCG2902.7903297E-919.4453687
TATACTG13550.019.081315
Produced by FastQC (version 0.11.5)