FastQCFastQC Report
Wed 25 May 2016
SRR1033131_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033131_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2016978
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14950.036.5364041
GTATCAA23650.033.05111
TCAACGC22200.032.803274
ATCAACG23250.031.3241653
CAACGCA23450.031.255055
AACGCAG26100.028.2504436
TATCAAC27350.027.335932
TATACCG700.008853408526.8472865
GTACATG50100.026.1285951
TACATGG49650.024.52872
ACGCAGA29550.024.157517
TACGACC1602.0465977E-623.4913734
CGCAGAG31150.022.4643758
ACATGGG53200.022.3449613
TAGACCG1100.00306685821.3557955
TATACGG2005.988768E-721.1422375
CTGTCGC5400.020.0032339
TCTGTCG5450.019.8197178
GAGTACA38850.019.1502781
ATAACCG1250.00642641518.7930985
Produced by FastQC (version 0.11.5)