##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033131_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2016978 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.619891739027395 33.0 31.0 34.0 30.0 34.0 2 31.702260510526145 34.0 31.0 34.0 30.0 34.0 3 31.881004651513305 34.0 31.0 34.0 30.0 34.0 4 35.237399713829305 37.0 35.0 37.0 33.0 37.0 5 35.326058588641025 37.0 35.0 37.0 33.0 37.0 6 35.3878212851107 37.0 36.0 37.0 33.0 37.0 7 35.350774772952406 37.0 37.0 37.0 33.0 37.0 8 35.33368584089663 37.0 37.0 37.0 33.0 37.0 9 37.00117502521098 39.0 38.0 39.0 34.0 39.0 10-11 36.99621463397221 39.0 38.0 39.0 34.0 39.0 12-13 36.94473092914251 39.0 38.0 39.0 34.0 39.0 14-15 38.41575986451018 41.0 39.0 41.0 34.0 41.0 16-17 38.42685443272063 41.0 39.0 41.0 34.0 41.0 18-19 38.42621610151424 41.0 39.0 41.0 34.0 41.0 20-21 38.46408884975443 41.0 39.0 41.0 34.0 41.0 22-23 38.4300974031447 41.0 39.0 41.0 34.0 41.0 24-25 38.42717520964533 41.0 39.0 41.0 34.0 41.0 26-27 38.38981287847463 41.0 39.0 41.0 34.0 41.0 28-29 38.366900630547285 40.5 39.0 41.0 34.0 41.0 30-31 38.36431111296206 41.0 39.0 41.0 34.0 41.0 32-33 38.42798235776493 41.0 39.0 41.0 34.0 41.0 34-35 38.40123937891241 41.0 39.0 41.0 34.0 41.0 36-37 38.321865186432376 41.0 39.0 41.0 34.0 41.0 38-39 38.2447094118032 41.0 39.0 41.0 33.0 41.0 40-41 38.18669142648061 41.0 39.0 41.0 33.0 41.0 42-43 38.082209374618856 40.5 38.0 41.0 33.0 41.0 44-45 37.957973017058194 40.0 38.0 41.0 33.0 41.0 46-47 37.8446086174465 40.0 38.0 41.0 33.0 41.0 48-49 37.73184362942977 40.0 38.0 41.0 32.5 41.0 50-51 37.622212289871285 40.0 38.0 41.0 32.0 41.0 52-53 37.48672171932465 40.0 37.5 41.0 32.0 41.0 54-55 37.34508779966861 40.0 37.0 41.0 31.5 41.0 56-57 37.20315863633614 40.0 37.0 41.0 31.0 41.0 58-59 37.01689780453728 40.0 36.5 41.0 31.0 41.0 60-61 36.81298035972628 40.0 36.0 41.0 31.0 41.0 62-63 36.5558744319472 39.0 35.5 41.0 31.0 41.0 64-65 36.2936231828012 39.0 35.0 41.0 30.5 41.0 66-67 36.007578912610846 38.5 35.0 41.0 30.0 41.0 68-69 35.70228579587878 38.0 35.0 40.0 30.0 41.0 70-71 35.35657503453186 37.0 35.0 40.0 29.5 41.0 72-73 34.99156039381688 37.0 35.0 39.5 29.0 41.0 74-75 34.62101768090679 36.0 34.0 39.0 29.0 41.0 76-77 34.28616524325005 36.0 34.0 39.0 29.0 40.5 78-79 33.909147496898825 35.0 34.0 37.5 29.0 39.5 80-81 33.538137996547306 35.0 34.0 37.0 28.0 39.0 82-83 33.30344406334625 35.0 34.0 37.0 28.5 39.0 84-85 33.01697192532591 35.0 34.0 36.0 28.0 38.0 86-87 32.748251344337916 35.0 34.0 36.0 28.0 37.0 88-89 32.37208710258615 35.0 34.0 36.0 27.0 37.0 90-91 32.17351255194653 35.0 34.0 35.0 27.0 36.5 92-93 31.994094382784542 35.0 34.0 35.0 26.5 36.0 94-95 31.81923972398311 35.0 34.0 35.0 26.0 36.0 96-97 31.642647316926613 35.0 33.0 35.0 25.5 36.0 98-99 31.449888149498904 35.0 33.0 35.0 25.0 35.5 100 31.290891125237856 35.0 33.0 35.0 25.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.15126860711855628 1101 2 0.17632039537603816 1101 3 0.1559511289100044 1101 4 0.23294126211999355 1101 5 0.17474046954949074 1101 6 0.13987825325011727 1101 7 0.2123364959702272 1101 8 0.1580940521957075 1101 9 0.22592747698971039 1101 10-11 0.22418857276642967 1101 12-13 0.23742347499190686 1101 14-15 0.22229183393999108 1101 16-17 0.11052114791774414 1101 18-19 0.09953738902418507 1101 20-21 0.14805715427055333 1101 22-23 0.18464904529486148 1101 24-25 0.16904773703696918 1101 26-27 0.2159327014609005 1101 28-29 0.20943667339855665 1101 30-31 0.17744111920937655 1101 32-33 0.20051399418673554 1101 34-35 0.22664429547631926 1101 36-37 0.18489488050808234 1101 38-39 0.15995982034904443 1101 40-41 0.19801823987656775 1101 42-43 0.18416488842829892 1101 44-45 0.1831838641824035 1101 46-47 0.2215237743253482 1101 48-49 0.15098263943712453 1101 50-51 0.09047277739001913 1101 52-53 0.1004115805493484 1101 54-55 0.058809878405590155 1101 56-57 0.1933788131758476 1101 58-59 0.19818325602800257 1101 60-61 0.1668903557165038 1101 62-63 0.1587709660430221 1101 64-65 0.2366737548510116 1101 66-67 0.2195535678940317 1101 68-69 0.11092983513437105 1101 70-71 0.2153953602628036 1101 72-73 0.2407327987879384 1101 74-75 0.13064126751442728 1101 76-77 0.15173429189714938 1101 78-79 0.25977352610969007 1101 80-81 0.22239854135679593 1101 82-83 0.20137147309280579 1101 84-85 0.1630136790008052 1101 86-87 0.20524182321010187 1101 88-89 0.3536919219590473 1101 90-91 0.3328109808779445 1101 92-93 0.3850776653772101 1101 94-95 0.39372998103128154 1101 96-97 0.28589041458450026 1101 98-99 0.3393604629578846 1101 100 0.35576854659951707 1102 1 0.07477192644227415 1102 2 0.07446213129478352 1102 3 0.13417055451576232 1102 4 0.13027306713161835 1102 5 0.009506604487562242 1102 6 0.04066768713428104 1102 7 0.08747778612847412 1102 8 0.02797775646545375 1102 9 0.14767262934427805 1102 10-11 0.12313526458672897 1102 12-13 0.12661915589107053 1102 14-15 0.07887137830908131 1102 16-17 0.1320410320200196 1102 18-19 0.1399896568307284 1102 20-21 0.12152942955950152 1102 22-23 0.15347118287566275 1102 24-25 0.15037355612188463 1102 26-27 0.1864050483899291 1102 28-29 0.13726178006267276 1102 30-31 0.2392744684627246 1102 32-33 0.1633352800465815 1102 34-35 0.17697582984764182 1102 36-37 0.18651175731898206 1102 38-39 0.18450500533965197 1102 40-41 0.20958612283259725 1102 42-43 0.14552849765554754 1102 44-45 0.010755146062244592 1102 46-47 -0.023443510038013926 1102 48-49 0.08677621252592616 1102 50-51 0.007704604689671157 1102 52-53 -0.03848958001995584 1102 54-55 -0.04355086123843677 1102 56-57 -0.09424267646021889 1102 58-59 -0.05520522026709784 1102 60-61 -0.017007740249361802 1102 62-63 0.09773162767896082 1102 64-65 -0.04060022069584335 1102 66-67 -0.17826578766364065 1102 68-69 -0.1978274681738128 1102 70-71 -0.12492785589919464 1102 72-73 -0.07345820560598781 1102 74-75 -0.030540953009655425 1102 76-77 -0.11566728259261083 1102 78-79 -0.12394467365469097 1102 80-81 -0.09397450048101064 1102 82-83 -0.07532241680434737 1102 84-85 -0.0922996269501013 1102 86-87 -0.09609277178464026 1102 88-89 -0.050080027765531554 1102 90-91 -0.07869312250664962 1102 92-93 -0.06391161249077726 1102 94-95 -0.09622100521382038 1102 96-97 -0.034791977079777325 1102 98-99 0.015883610663809833 1102 100 -0.0030079602301675834 1103 1 -0.06191274863214247 1103 2 -0.011783469358260135 1103 3 0.006720684608342964 1103 4 -0.04668678100129853 1103 5 -0.1542141007843867 1103 6 -0.009864583664757731 1103 7 0.055532457513265854 1103 8 0.10262327567920693 1103 9 0.12361553772124978 1103 10-11 0.08073425825701719 1103 12-13 0.06019140650233368 1103 14-15 0.11452652016830456 1103 16-17 0.06757387155592909 1103 18-19 0.11069289757943324 1103 20-21 0.003173494909759711 1103 22-23 0.056166039773813736 1103 24-25 -0.0047031851209951014 1103 26-27 -4.248935511128593E-4 1103 28-29 8.635046257978729E-4 1103 30-31 -0.04876423049672951 1103 32-33 0.030793422924745073 1103 34-35 0.07071630708956178 1103 36-37 0.06929568605768566 1103 38-39 0.024760678848366524 1103 40-41 0.037202814256040995 1103 42-43 -0.0014383664918540262 1103 44-45 0.028229347393178728 1103 46-47 0.0528462083464305 1103 48-49 0.06629953456099003 1103 50-51 -0.10510949099958822 1103 52-53 -0.11341528577939641 1103 54-55 -0.16646959495296443 1103 56-57 -0.1145397721743322 1103 58-59 -0.05567084370784414 1103 60-61 0.004828529486765376 1103 62-63 0.02829602494214356 1103 64-65 -0.023255060503210245 1103 66-67 -0.013139609326877633 1103 68-69 -0.03913315420429342 1103 70-71 0.057802281802040056 1103 72-73 0.04962708676299599 1103 74-75 0.015514307185078735 1103 76-77 -0.05609854763546451 1103 78-79 -0.1268635125138502 1103 80-81 -0.1528241895396647 1103 82-83 -0.07115892837312288 1103 84-85 -0.058293822010597296 1103 86-87 -0.13913026158083142 1103 88-89 -0.06318260208844606 1103 90-91 -0.09528040497674084 1103 92-93 -0.09200745277799172 1103 94-95 -0.012838684040939796 1103 96-97 -0.003625913560952654 1103 98-99 -0.005462661194325591 1103 100 -0.03201449640279108 1104 1 0.0739374459122395 1104 2 0.05021059542099238 1104 3 0.13628524118630025 1104 4 0.11312162035625306 1104 5 0.09783825983169692 1104 6 0.065734557650174 1104 7 0.060267743596583045 1104 8 0.12451085643687776 1104 9 0.09730306700993907 1104 10-11 0.1449662465559669 1104 12-13 0.0924453803406422 1104 14-15 0.03374767252441302 1104 16-17 0.09413088974272199 1104 18-19 0.07861552843559849 1104 20-21 0.09834504345138839 1104 22-23 0.13400915430813143 1104 24-25 0.032667322220781614 1104 26-27 0.11803730071218865 1104 28-29 0.16231195629823958 1104 30-31 0.19135067656824845 1104 32-33 0.19009551095533084 1104 34-35 0.11904165714611992 1104 36-37 0.16577711092018887 1104 38-39 0.10405164939237466 1104 40-41 0.08172999997763952 1104 42-43 0.10126086989643568 1104 44-45 0.1319937962864728 1104 46-47 0.04681384338387318 1104 48-49 0.0055007508298388075 1104 50-51 -0.009387419787181273 1104 52-53 -0.016119594608184684 1104 54-55 -0.11287123358393103 1104 56-57 0.03259357763280235 1104 58-59 -0.02362232697787192 1104 60-61 -0.009962637207131309 1104 62-63 -0.07639181753484792 1104 64-65 -0.09785681575377225 1104 66-67 -0.06802364821806606 1104 68-69 -0.09543712741436394 1104 70-71 -0.08939005257696664 1104 72-73 0.013011512136927195 1104 74-75 -0.11703740494973403 1104 76-77 -0.15581714352809684 1104 78-79 -0.1258255236573973 1104 80-81 0.0034759677059881255 1104 82-83 0.07134419071241638 1104 84-85 0.09103847728945169 1104 86-87 0.11869595567691249 1104 88-89 0.0798674331161564 1104 90-91 0.0472861196150518 1104 92-93 0.02847219998785988 1104 94-95 0.04255422766555128 1104 96-97 0.05482035137773522 1104 98-99 0.07626133568402693 1104 100 0.09379738492816259 1105 1 -0.03478578055471715 1105 2 -0.08861592799051721 1105 3 -0.07023696060005946 1105 4 -0.07096334703722107 1105 5 -0.057351789968585365 1105 6 -0.12052791202273028 1105 7 -0.15698941001365796 1105 8 -0.10320592489215841 1105 9 -0.09948780540231894 1105 10-11 -0.13377935979553257 1105 12-13 -0.01866088938736965 1105 14-15 -0.048235288296233136 1105 16-17 -0.03924638940993219 1105 18-19 -0.05967961273240974 1105 20-21 -0.04828621266723587 1105 22-23 -0.017486198337465453 1105 24-25 0.05415055522920653 1105 26-27 0.032622757595625274 1105 28-29 0.0427624886885809 1105 30-31 0.048882101376094056 1105 32-33 -0.0253680035602315 1105 34-35 0.019677098011932515 1105 36-37 -0.044831940051736296 1105 38-39 0.0190045037797546 1105 40-41 -0.10257843022692015 1105 42-43 -0.12885743658029014 1105 44-45 -0.11807845108852888 1105 46-47 -0.06916817206027304 1105 48-49 -0.053345128157488375 1105 50-51 0.008531833410188483 1105 52-53 -0.04365346126529346 1105 54-55 -0.04729357941699419 1105 56-57 -0.08414832338046097 1105 58-59 -0.032335584427187314 1105 60-61 -0.05098301656745008 1105 62-63 -0.11407481621534998 1105 64-65 -0.08357412577677792 1105 66-67 -0.06672588374227217 1105 68-69 -0.05173687449254061 1105 70-71 -0.1132555447664032 1105 72-73 -0.08556639069539074 1105 74-75 -0.1013345219994477 1105 76-77 -0.08121932058232062 1105 78-79 -0.10504012188359013 1105 80-81 -0.2032655524683875 1105 82-83 -0.13120194119672135 1105 84-85 -0.1390784850500637 1105 86-87 -0.05988555392359274 1105 88-89 -0.016973508407154725 1105 90-91 0.0023522847691950233 1105 92-93 -0.0030960945036468956 1105 94-95 -0.007439611705571281 1105 96-97 0.017731237320973037 1105 98-99 0.010797149099861514 1105 100 0.09089785694434482 1106 1 0.16693300537928835 1106 2 0.16901484884803608 1106 3 0.13884726412156567 1106 4 0.12754957219959806 1106 5 0.07046103630543854 1106 6 0.13492714678352513 1106 7 0.08308521934984725 1106 8 0.09281341050485281 1106 9 0.11632396707745585 1106 10-11 0.06933402997770344 1106 12-13 0.023907431786660993 1106 14-15 0.08610587975263684 1106 16-17 0.1394806789582077 1106 18-19 0.12924933929296145 1106 20-21 0.12630196000385752 1106 22-23 0.12285150169179104 1106 24-25 0.16215636837291214 1106 26-27 0.20277263175574944 1106 28-29 0.18931455163119892 1106 30-31 0.15173360816152126 1106 32-33 0.18091438090839063 1106 34-35 0.12895248489801503 1106 36-37 0.2006059730037606 1106 38-39 0.20127517126555716 1106 40-41 0.13065760349017808 1106 42-43 0.18971163822938308 1106 44-45 0.1669626371699735 1106 46-47 0.22143972470244933 1106 48-49 0.23524787296433658 1106 50-51 0.24214522480297518 1106 52-53 0.16216178787175295 1106 54-55 0.23209767085158006 1106 56-57 0.2549719527654659 1106 58-59 0.2352877208373556 1106 60-61 0.2636781659063061 1106 62-63 0.3139842372916988 1106 64-65 0.3054617172640235 1106 66-67 0.42752688867596333 1106 68-69 0.3580830627332787 1106 70-71 0.28983706064271075 1106 72-73 0.3856747975439987 1106 74-75 0.348561394223907 1106 76-77 0.31792409368945584 1106 78-79 0.3547939595068996 1106 80-81 0.3451132671308912 1106 82-83 0.3182406059621883 1106 84-85 0.24646304750796588 1106 86-87 0.2597544824841407 1106 88-89 0.3507627342332853 1106 90-91 0.3011502467138314 1106 92-93 0.3283305118577786 1106 94-95 0.301222339519736 1106 96-97 0.2506106437411262 1106 98-99 0.31588937229262015 1106 100 0.3652559979908183 1107 1 -0.027988082823881655 1107 2 -0.0767702272876356 1107 3 -0.06933747038214833 1107 4 -0.11106964655575524 1107 5 -0.16533436647049626 1107 6 -0.1354011158365651 1107 7 -0.13541782496286459 1107 8 -0.19812950015820263 1107 9 -0.20382260311281897 1107 10-11 -0.19490706505227706 1107 12-13 -0.16622848687561032 1107 14-15 -0.2593194560974652 1107 16-17 -0.23442544118756814 1107 18-19 -0.16264308704264607 1107 20-21 -0.1909329599722298 1107 22-23 -0.1474176667632321 1107 24-25 -0.1505744828527682 1107 26-27 -0.09963881628871718 1107 28-29 -0.11780881001561738 1107 30-31 -0.17879739482422963 1107 32-33 -0.1765483582680787 1107 34-35 -0.14484546428975165 1107 36-37 -0.19076400884730305 1107 38-39 -0.14309193647440566 1107 40-41 -0.10433797147626223 1107 42-43 -0.12126174732063077 1107 44-45 -0.1892552717046314 1107 46-47 -0.14872669800337235 1107 48-49 -0.1324980319141602 1107 50-51 -0.1159548983107328 1107 52-53 -0.18587452083284006 1107 54-55 -0.16786704967154265 1107 56-57 -0.11836280229885432 1107 58-59 -0.10102081713967692 1107 60-61 -0.14853315649406795 1107 62-63 -0.036256830456032674 1107 64-65 -0.1372666129577098 1107 66-67 -0.13443977346796743 1107 68-69 -0.10952896132049261 1107 70-71 -0.1247804095501408 1107 72-73 -0.14816585355602285 1107 74-75 -0.08198031339649248 1107 76-77 -0.1318692954072418 1107 78-79 -0.17711350538257875 1107 80-81 -0.16348141947582917 1107 82-83 -0.11496262996139706 1107 84-85 -0.1586925877792993 1107 86-87 -0.21741210485230056 1107 88-89 -0.17724451576169287 1107 90-91 -0.19994583290115742 1107 92-93 -0.16308708895660828 1107 94-95 -0.2283716538890026 1107 96-97 -0.2342509410633724 1107 98-99 -0.12967001099130115 1107 100 -0.12134180982912213 1108 1 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0.06733918719088905 2214 42-43 0.042079387371458665 2214 44-45 0.1538873681085562 2214 46-47 0.1333920924612073 2214 48-49 0.08340189120448827 2214 50-51 0.08108817260920631 2214 52-53 0.15468880324240786 2214 54-55 0.08396344853346704 2214 56-57 0.03143215704358937 2214 58-59 0.12257549570546189 2214 60-61 0.1735803036060517 2214 62-63 0.12339878131809456 2214 64-65 0.08208879625687615 2214 66-67 0.11877123030949832 2214 68-69 0.09219928933605104 2214 70-71 0.1666889104450746 2214 72-73 0.19506047051248743 2214 74-75 0.1213815355207899 2214 76-77 0.12228548080521762 2214 78-79 0.1644221974802491 2214 80-81 0.12446822803033797 2214 82-83 0.10100778907754204 2214 84-85 0.025804439010194358 2214 86-87 0.0637421818736783 2214 88-89 0.045346494898375056 2214 90-91 -0.028299367948967813 2214 92-93 -0.07034434507891518 2214 94-95 -0.010585182141500837 2214 96-97 0.0012641160776993843 2214 98-99 -0.10100331465878654 2214 100 -0.03785547441484738 2215 1 -0.220996266345999 2215 2 -0.16958446871552013 2215 3 -0.16002921246437296 2215 4 -0.17972954839749633 2215 5 -0.15334891923251348 2215 6 -0.08570170087153173 2215 7 -0.19488502738826696 2215 8 -0.13488668383112667 2215 9 -0.13794949010613777 2215 10-11 -0.288066927702225 2215 12-13 -0.22965048534552324 2215 14-15 -0.2617305995857322 2215 16-17 -0.23122876575202866 2215 18-19 -0.29227126003110726 2215 20-21 -0.22551685539843191 2215 22-23 -0.23697559705134097 2215 24-25 -0.2125398758089645 2215 26-27 -0.28058880709812684 2215 28-29 -0.21256073052823865 2215 30-31 -0.17919027539375776 2215 32-33 -0.09221623002518697 2215 34-35 -0.20673343625867346 2215 36-37 -0.280774363732462 2215 38-39 -0.15639651998937154 2215 40-41 -0.21526551524158322 2215 42-43 -0.2568421228192932 2215 44-45 -0.10168768877502288 2215 46-47 -0.08041988903610786 2215 48-49 -0.09071270119727615 2215 50-51 -0.18331674952034405 2215 52-53 -0.32184465989266187 2215 54-55 -0.28557156552908936 2215 56-57 -0.30359423329448276 2215 58-59 -0.33470655577211517 2215 60-61 -0.34386392379609987 2215 62-63 -0.2741337993854174 2215 64-65 -0.3747739413652553 2215 66-67 -0.33614422698534696 2215 68-69 -0.2871852368896981 2215 70-71 -0.27030619614782836 2215 72-73 -0.22251072027870578 2215 74-75 -0.231866748863645 2215 76-77 -0.30665361447155703 2215 78-79 -0.3251174511484578 2215 80-81 -0.2903883524816919 2215 82-83 -0.3017191489781368 2215 84-85 -0.35487086048910754 2215 86-87 -0.4132786308868788 2215 88-89 -0.3032146502376847 2215 90-91 -0.28711819750496304 2215 92-93 -0.2016512207162222 2215 94-95 -0.227732483819981 2215 96-97 -0.3299769266912129 2215 98-99 -0.4761314959982599 2215 100 -0.29651272960787267 2216 1 -0.06008531551305296 2216 2 -0.06229789023129584 2216 3 -0.07682644987499998 2216 4 -0.07470102846968985 2216 5 -0.14837646720953046 2216 6 -0.11049062288171996 2216 7 -0.08588765486844352 2216 8 -0.17214830486832255 2216 9 -0.23069801430731474 2216 10-11 -0.21386222040801073 2216 12-13 -0.25376080898906395 2216 14-15 -0.254804165014491 2216 16-17 -0.21612320466732626 2216 18-19 -0.2769938805389458 2216 20-21 -0.27644828145908207 2216 22-23 -0.3261039258823786 2216 24-25 -0.2938980605583339 2216 26-27 -0.3770131929417033 2216 28-29 -0.3071572062828807 2216 30-31 -0.4365583451235935 2216 32-33 -0.30159025355603575 2216 34-35 -0.3537267346178439 2216 36-37 -0.29017340897154753 2216 38-39 -0.3010908735979214 2216 40-41 -0.3454684039435776 2216 42-43 -0.36933173057690283 2216 44-45 -0.26185802917468237 2216 46-47 -0.15328503126625037 2216 48-49 -0.2469439499773216 2216 50-51 -0.19126670449733751 2216 52-53 -0.2762432191563562 2216 54-55 -0.25689343466275005 2216 56-57 -0.2405184428213616 2216 58-59 -0.322708867569105 2216 60-61 -0.22670067863717946 2216 62-63 -0.21633995898294955 2216 64-65 -0.19255224662045833 2216 66-67 -0.28957858697182104 2216 68-69 -0.27531241624836156 2216 70-71 -0.260244898987132 2216 72-73 -0.11184693412073443 2216 74-75 -0.17148623414743014 2216 76-77 -0.22221555062964882 2216 78-79 -0.22372647711722493 2216 80-81 -0.1175363436361394 2216 82-83 -0.15128136949208226 2216 84-85 -0.1633297642410767 2216 86-87 -0.1665886454340182 2216 88-89 -0.10607748709694675 2216 90-91 -0.14189196190248765 2216 92-93 -0.09423885091545259 2216 94-95 -0.15810518276665775 2216 96-97 -0.15553381031175206 2216 98-99 -0.186577035025298 2216 100 -0.14234189600343683 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 20747.0 3 3684.0 4 2341.0 5 1894.0 6 1552.0 7 1839.0 8 2127.0 9 2425.0 10 2539.0 11 2769.0 12 3150.0 13 3649.0 14 4097.0 15 4385.0 16 4222.0 17 4395.0 18 4197.0 19 4087.0 20 4178.0 21 4248.0 22 5024.0 23 6897.0 24 10562.0 25 14690.0 26 14990.0 27 12902.0 28 13647.0 29 16971.0 30 20553.0 31 24848.0 32 32497.0 33 45007.0 34 61564.0 35 93981.0 36 172271.0 37 383518.0 38 675943.0 39 327684.0 40 904.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.556088252879896 20.315980488620188 13.563353784533163 23.564577473966754 2 15.127168324419952 20.48626657546459 41.03989230477511 23.346672795340353 3 17.89480107819493 25.840510301941215 30.167925933386286 26.096762686477565 4 11.862609428861939 17.044515537038414 40.110755648141264 30.982119385958384 5 12.310505073451838 38.3454451032026 36.29151180779759 13.05253801554797 6 30.15876242756424 39.039323438717126 18.092292344208882 12.70962178950975 7 26.608496373281532 32.72812825230795 23.20653577854829 17.456839595862235 8 24.163066271891832 37.42909747074023 20.857182384685302 17.550653872682638 9 24.192810969598753 17.480913683634263 21.769855422021315 36.55641992474566 10-11 23.088683385059174 27.544684355027112 29.580897979059902 19.78573428085382 12-13 23.877759083473293 24.837366989031846 29.449132896670083 21.835741030824778 14-15 21.239329494691184 27.013309788735835 26.451993294649377 25.295367421923597 16-17 20.903814440888105 30.298973041820922 26.910053201288413 21.88715931600256 18-19 20.69765175777865 29.336750316809013 28.917726849759262 21.047871075653074 20-21 22.152321197796976 28.586433338523843 28.26476244506576 20.99648301861342 22-23 21.55810243713526 28.49629414019441 28.404806733473137 21.540796689197197 24-25 21.536423341863838 28.499468258807326 28.373010805665395 21.59109759366344 26-27 21.268151489297978 28.44310820490582 28.484464851685097 21.804275454111107 28-29 21.28892947402115 28.413727681310952 28.569607978532307 21.727734866135595 30-31 21.595030601284748 29.08490703576646 28.005283197013558 21.31477916593523 32-33 21.13510587625029 28.68665048501561 28.68977473882106 21.48846889991304 34-35 21.222660565562084 28.325830918478058 28.788174052057734 21.663334463902128 36-37 21.697586730176944 28.812246611131126 28.105131604163358 21.38503505452858 38-39 21.48248899317351 28.892380286041124 28.32561363770471 21.29951708308065 40-41 21.165439677111056 28.587472434571232 28.623519772113394 21.62356811620432 42-43 21.155564877186276 28.47859830440529 28.639743516322397 21.726093302086035 44-45 21.34519531487264 28.616315637461682 28.575533361513184 21.462955686152497 46-47 21.37388664374112 28.906195574862092 28.338717638696785 21.3812001427 48-49 21.22425514413698 28.626517099277176 28.467856472622245 21.6813712839636 50-51 21.299879292354216 28.51918319424312 28.452916704782915 21.72802080861975 52-53 21.29007642671633 28.51945177441475 28.50113098053682 21.6893408183321 54-55 21.482255126848965 28.976008555676724 28.1960269353091 21.34570938216521 56-57 21.276725498192167 28.65729510503032 28.30869383601461 21.757285560762895 58-59 21.055194112998148 28.296465200865878 28.76891871657648 21.879421969559495 60-61 21.505225468484934 28.722016777724036 28.213959984639796 21.55879776915123 62-63 21.275881046347898 28.74861174464034 28.329085933006766 21.646421276004997 64-65 21.29155425346437 28.40180343706129 28.521019481870063 21.78562282760428 66-67 21.143108726418777 28.46621252953424 28.329421050765802 22.061257693281178 68-69 21.653858358288876 28.681206988874123 28.199655910993 21.465278741844 70-71 21.570464837303597 28.432968596107504 28.348632563872396 21.647934002716504 72-73 21.311394130953488 28.562659973350684 28.51275758420873 21.613188311487093 74-75 21.391874994422228 28.585863493798016 28.358711787296958 21.663549724482802 76-77 21.397544244990442 28.63108887671396 28.19618770436736 21.77517917392824 78-79 21.542823512845914 28.749431059256107 28.25820836469325 21.44953706320473 80-81 21.43442551830651 28.474593498454315 28.39744559114088 21.69353539209829 82-83 21.24438750416475 28.601541552301324 28.530839970489772 21.623230973044155 84-85 21.34741324194631 28.481992189562632 28.34522400902566 21.8253705594654 86-87 21.537976031317 28.642038232982063 28.246138239749797 21.573847495951135 88-89 21.3244013735619 28.5372224152254 28.29922580648379 21.83915040472891 90-91 21.273542198244577 28.36671829675578 28.39734716121807 21.962392343781566 92-93 21.444079499520257 28.678868059398923 28.175455232697495 21.70159720838333 94-95 21.2759409930159 28.42121354644902 28.37260964307428 21.930235817460797 96-97 21.249656480877892 28.401188439004223 28.384928949522266 21.96422613059562 98-99 21.3223588532305 28.480566322673056 28.296009727312082 21.90106509678436 100 21.433997082330645 28.469669954239972 28.22503460056608 21.871298362863307 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 356.0 1 342.5 2 306.5 3 274.0 4 232.0 5 191.0 6 169.0 7 157.5 8 151.5 9 145.0 10 151.5 11 163.5 12 189.0 13 197.5 14 223.0 15 276.5 16 363.0 17 523.5 18 761.5 19 1071.0 20 1608.0 21 2420.0 22 3573.0 23 5492.5 24 7868.0 25 10535.5 26 14342.5 27 19160.0 28 25177.0 29 31698.5 30 37616.0 31 43878.5 32 50280.0 33 56240.5 34 62094.5 35 66855.5 36 70278.5 37 73601.0 38 76013.5 39 76318.5 40 76114.5 41 75834.0 42 74261.5 43 72557.5 44 70427.0 45 67902.5 46 66451.0 47 64612.0 48 62321.5 49 60490.0 50 58501.0 51 55915.0 52 52065.5 53 48858.0 54 46786.0 55 44380.5 56 41016.0 57 37049.0 58 33625.0 59 30484.0 60 26753.5 61 22714.0 62 19239.0 63 15854.0 64 12773.5 65 10173.5 66 7833.5 67 5787.5 68 4130.5 69 3036.0 70 2182.0 71 1560.0 72 1186.5 73 878.0 74 644.0 75 438.5 76 305.0 77 217.0 78 155.0 79 98.5 80 63.0 81 45.5 82 28.0 83 20.5 84 17.0 85 15.0 86 10.5 87 7.0 88 8.5 89 7.0 90 3.5 91 2.0 92 2.5 93 2.5 94 3.5 95 3.0 96 1.0 97 1.0 98 2.0 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3303952745146452 2 0.11918821127449085 3 0.06876624336011598 4 0.041051513700199015 5 0.04437331492956294 6 0.009469612459828516 7 0.006990656318512151 8 0.014923315970724521 9 0.01735269298921456 10-11 0.020649704657165324 12-13 0.015914898427251065 14-15 0.018815277112591216 16-17 0.01941022658650714 18-19 0.019063172726722852 20-21 0.023599662465331798 22-23 0.015220790707682483 24-25 0.024268980623487216 26-27 0.01851780237563325 28-29 0.018170748515848958 30-31 0.019633332639225613 32-33 0.02461603448327151 34-35 0.014576262110940229 36-37 0.00949440202124168 38-39 0.01363425877724001 40-41 0.009196927284283715 42-43 0.008106186582104515 44-45 0.008453240441888806 46-47 0.007907870090799206 48-49 0.004685227107087931 50-51 0.006023863423398767 52-53 0.007139393686991132 54-55 0.003544907282082402 56-57 0.002999536930992802 58-59 0.0029747473695796384 60-61 0.0038423820190403666 62-63 0.0028755891239269837 64-65 0.0025533248255558564 66-67 0.0019583753516399286 68-69 0.0028755891239269837 70-71 0.00230542921142422 72-73 0.002007954474466256 74-75 0.0021814814043584012 76-77 0.004115067194585167 78-79 0.003569696843495566 80-81 0.0036192759663218935 82-83 0.0024789561413163653 84-85 0.0030491160538191294 86-87 0.0027020621940348384 88-89 0.6007750208480211 90-91 0.6114593218170947 92-93 0.6079144145350123 94-95 0.594701578301796 96-97 0.594701578301796 98-99 0.5900907198789476 100 0.5926192551430903 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2016978.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.46593044779877 #Duplication Level Percentage of deduplicated Percentage of total 1 83.42594655855638 62.95816680529792 2 11.32759947174837 17.096956677509688 3 2.7570862400899836 6.241982352996412 4 0.9886221964257511 2.9842917565846316 5 0.4743671405449376 1.7899278817542723 6 0.27590178622258066 1.2492711005698032 7 0.17147139682637988 0.9058173954680542 8 0.11691940592531856 0.7058745404446435 9 0.08087887896085477 0.5493239868920221 >10 0.36703504188917485 4.66742313158577 >50 0.01173506086511745 0.5837031731214465 >100 0.002371060120021767 0.24090885017975486 >500 6.576182517751246E-5 0.026352347595571885 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 4.957912282632731E-5 0.0 0.0 0.0 0.0 10-11 4.957912282632731E-5 0.0 0.0 0.0 0.0 12-13 4.957912282632731E-5 0.0 0.0 0.0 0.0 14-15 4.957912282632731E-5 0.0 0.0 0.0 0.0 16-17 4.957912282632731E-5 0.0 0.0 4.957912282632731E-5 0.0 18-19 4.957912282632731E-5 0.0 0.0 4.957912282632731E-5 0.0 20-21 4.957912282632731E-5 0.0 0.0 1.7352692989214556E-4 0.0 22-23 4.957912282632731E-5 0.0 0.0 3.222642983711275E-4 0.0 24-25 4.957912282632731E-5 0.0 0.0 3.470538597842912E-4 0.0 26-27 4.957912282632731E-5 0.0 0.0 5.453703510896004E-4 0.0 28-29 4.957912282632731E-5 0.0 0.0 0.0012394780706581827 0.0 30-31 4.957912282632731E-5 0.0 0.0 0.004784385352740585 0.0 32-33 4.957912282632731E-5 0.0 0.0 0.011576725179947426 0.0 34-35 4.957912282632731E-5 0.0 0.0 0.02186439316641034 0.0 36-37 4.957912282632731E-5 0.0 0.0 0.040134299927911954 0.0 38-39 4.957912282632731E-5 0.0 0.0 0.07174099072969561 0.0 40-41 4.957912282632731E-5 0.0 0.0 0.10994170486738081 0.0 42-43 4.957912282632731E-5 0.0 0.0 0.15047263777790337 0.0 44-45 4.957912282632731E-5 0.0 0.0 0.1954161126199691 0.0 46-47 4.957912282632731E-5 0.0 0.0 0.24861451141261828 0.0 48-49 4.957912282632731E-5 0.0 0.0 0.31182789301618563 0.0 50-51 4.957912282632731E-5 0.0 0.0 0.38317225076327055 0.0 52-53 4.957912282632731E-5 0.0 0.0 0.45245907491306303 0.0 54-55 4.957912282632731E-5 0.0 0.0 0.5283151328373438 0.0 56-57 4.957912282632731E-5 0.0 0.0 0.6180037660301698 0.0 58-59 4.957912282632731E-5 2.4789561413163655E-5 0.0 0.7229379794920916 0.0 60-61 4.957912282632731E-5 4.957912282632731E-5 0.0 0.8320120497100117 0.0 62-63 4.957912282632731E-5 4.957912282632731E-5 0.0 0.9428709683496797 0.0 64-65 4.957912282632731E-5 9.915824565265462E-5 0.0 1.0627780769051522 0.0 66-67 4.957912282632731E-5 9.915824565265462E-5 0.0 1.200137036695492 0.0 68-69 4.957912282632731E-5 9.915824565265462E-5 0.0 1.3601536556174634 0.0 70-71 4.957912282632731E-5 9.915824565265462E-5 0.0 1.5273592473492523 0.0 72-73 4.957912282632731E-5 9.915824565265462E-5 0.0 1.7050756131202225 0.0 74-75 4.957912282632731E-5 9.915824565265462E-5 0.0 1.881949133803145 0.0 76-77 4.957912282632731E-5 9.915824565265462E-5 0.0 2.070250642297536 0.0 78-79 4.957912282632731E-5 9.915824565265462E-5 0.0 2.2908033701904533 0.0 80-81 4.957912282632731E-5 9.915824565265462E-5 0.0 2.5312373263367274 0.0 82-83 4.957912282632731E-5 9.915824565265462E-5 0.0 2.7817854235395725 0.0 84-85 4.957912282632731E-5 9.915824565265462E-5 0.0 3.0303503558293645 0.0 86-87 4.957912282632731E-5 9.915824565265462E-5 0.0 3.287889109350722 0.0 88 4.957912282632731E-5 9.915824565265462E-5 0.0 3.4949315262734646 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1495 0.0 36.536404 1 GTATCAA 2365 0.0 33.0511 1 TCAACGC 2220 0.0 32.80327 4 ATCAACG 2325 0.0 31.324165 3 CAACGCA 2345 0.0 31.25505 5 AACGCAG 2610 0.0 28.250443 6 TATCAAC 2735 0.0 27.33593 2 TATACCG 70 0.0088534085 26.847286 5 GTACATG 5010 0.0 26.128595 1 TACATGG 4965 0.0 24.5287 2 ACGCAGA 2955 0.0 24.15751 7 TACGACC 160 2.0465977E-6 23.491373 4 CGCAGAG 3115 0.0 22.464375 8 ACATGGG 5320 0.0 22.344961 3 TAGACCG 110 0.003066858 21.355795 5 TATACGG 200 5.988768E-7 21.142237 5 CTGTCGC 540 0.0 20.003233 9 TCTGTCG 545 0.0 19.819717 8 GAGTACA 3885 0.0 19.150278 1 ATAACCG 125 0.006426415 18.793098 5 >>END_MODULE