FastQCFastQC Report
Wed 25 May 2016
SRR1033131_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033131_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2016978
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39200.19435016147920306No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34300.17005639129430267No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28750.054.176121
GTATCAA53200.043.1644251
TCAACGC62450.036.0579154
ATCAACG62950.035.771513
CAACGCA64300.035.0204775
AACGCAG67150.033.594146
TATCAAC68350.033.2901042
ACGCAGA76600.029.265637
CGCAGAG77250.028.9578298
GCAGAGT86800.025.4469479
GTACATG50700.023.9458071
GTGTACG1201.823857E-423.5281471
TATAACG1602.0355674E-623.5059762
TACATGG50900.022.536182
GTGGTAT14200.022.202621
CAGAGTA85750.021.34670610-11
GAGTACT61550.021.3408312-13
AGAGTAC81250.020.90944310-11
ACATGGG53200.020.6776773
TGGTATC13950.019.5461882
Produced by FastQC (version 0.11.5)