##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033131_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2016978
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.76688243500921	34.0	31.0	34.0	31.0	34.0
2	32.87051420491448	34.0	31.0	34.0	31.0	34.0
3	32.9648127049477	34.0	31.0	34.0	31.0	34.0
4	36.38120544696075	37.0	37.0	37.0	35.0	37.0
5	36.32090037670218	37.0	37.0	37.0	35.0	37.0
6	36.407429828188505	37.0	37.0	37.0	35.0	37.0
7	36.377351165952234	37.0	37.0	37.0	35.0	37.0
8	36.378300606154355	37.0	37.0	37.0	35.0	37.0
9	38.199886662125216	39.0	39.0	39.0	37.0	39.0
10-11	38.184821301967595	39.0	39.0	39.0	37.0	39.0
12-13	38.14687517662563	39.0	39.0	39.0	37.0	39.0
14-15	39.68457043160609	41.0	40.0	41.0	37.0	41.0
16-17	39.698586449629104	41.0	40.0	41.0	37.0	41.0
18-19	39.7185333702202	41.0	40.0	41.0	37.0	41.0
20-21	39.71574826299543	41.0	40.0	41.0	37.0	41.0
22-23	39.64606827640162	41.0	40.0	41.0	37.0	41.0
24-25	39.600088597892494	41.0	40.0	41.0	37.0	41.0
26-27	39.49898313219083	41.0	39.5	41.0	36.5	41.0
28-29	39.41017799896677	41.0	39.0	41.0	36.0	41.0
30-31	39.31579571021598	41.0	39.0	41.0	36.0	41.0
32-33	39.34164452958832	41.0	39.0	41.0	36.0	41.0
34-35	39.28285980313122	41.0	39.0	41.0	35.0	41.0
36-37	39.19570342363675	41.0	39.0	41.0	35.0	41.0
38-39	39.102814705961094	41.0	39.0	41.0	35.0	41.0
40-41	38.989947832846966	41.0	39.0	41.0	35.0	41.0
42-43	38.875080194231174	41.0	39.0	41.0	35.0	41.0
44-45	38.731170097046174	41.0	39.0	41.0	35.0	41.0
46-47	38.52471866326752	41.0	39.0	41.0	35.0	41.0
48-49	38.347250441006295	41.0	39.0	41.0	35.0	41.0
50-51	38.22203563945665	41.0	38.5	41.0	34.0	41.0
52-53	38.09495641499312	40.5	38.0	41.0	34.0	41.0
54-55	37.96661788080981	40.0	38.0	41.0	34.0	41.0
56-57	37.8214849145603	40.0	37.5	41.0	34.0	41.0
58-59	37.64569965562342	40.0	37.0	41.0	33.5	41.0
60-61	37.465852874944595	40.0	37.0	41.0	33.0	41.0
62-63	37.24255742997693	40.0	36.0	41.0	33.0	41.0
64-65	36.99949107030419	39.0	36.0	41.0	33.0	41.0
66-67	36.73020801416773	39.0	35.0	41.0	32.5	41.0
68-69	36.41849960683756	38.5	35.0	41.0	32.0	41.0
70-71	36.086322954439765	37.5	35.0	40.0	32.0	41.0
72-73	35.73299733561794	37.0	35.0	40.0	31.0	41.0
74-75	35.393560316473454	36.5	35.0	39.0	31.0	41.0
76-77	35.04745713636936	36.0	35.0	39.0	31.0	41.0
78-79	34.69692827586617	36.0	35.0	38.5	31.0	39.5
80-81	34.34359224542855	35.0	35.0	37.0	31.0	39.0
82-83	34.00774153213372	35.0	35.0	37.0	30.5	39.0
84-85	33.721103799843135	35.0	34.0	36.5	30.0	38.0
86-87	33.47344616550106	35.0	34.0	36.0	30.0	37.0
88-89	33.24269005413049	35.0	34.0	36.0	30.0	37.0
90-91	33.03454425382924	35.0	34.0	35.5	30.0	36.5
92-93	32.859477892173345	35.0	34.0	35.0	30.0	36.0
94-95	32.70364996544335	35.0	34.0	35.0	29.0	36.0
96-97	32.57766123378639	35.0	34.0	35.0	29.0	36.0
98-99	32.471558936190675	35.0	34.0	35.0	29.0	36.0
100	32.37434320057036	35.0	34.0	35.0	29.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.025094829000018137
1101	2	0.09062968734743038
1101	3	0.10993938464685016
1101	4	0.06250910118505004
1101	5	0.016087284448559558
1101	6	0.06089022615412887
1101	7	0.06477901039648515
1101	8	0.06689287787826004
1101	9	0.1531208553468275
1101	10-11	0.12411299465902914
1101	12-13	0.1326755850700394
1101	14-15	0.1337296510965018
1101	16-17	0.13235283873232362
1101	18-19	0.12410618479410118
1101	20-21	0.1554986537040861
1101	22-23	0.14789583723811717
1101	24-25	0.07156323324723957
1101	26-27	0.07934018386893626
1101	28-29	0.13743266577061775
1101	30-31	0.14331422857170395
1101	32-33	0.10880056732088406
1101	34-35	0.13793043010073802
1101	36-37	0.1701144910615895
1101	38-39	0.16694425516568856
1101	40-41	0.19766734523278728
1101	42-43	0.11703120767721487
1101	44-45	0.10704238613821104
1101	46-47	0.10376839276579375
1101	48-49	0.12040996859626318
1101	50-51	0.18241604583488424
1101	52-53	0.19636429631396624
1101	54-55	0.17625648411987527
1101	56-57	0.143863265768033
1101	58-59	0.14735652949897116
1101	60-61	0.23522050204007883
1101	62-63	0.03694821498771006
1101	64-65	0.08398023525350595
1101	66-67	0.10715412383675016
1101	68-69	0.1616330624233271
1101	70-71	0.1566523278221439
1101	72-73	0.15724307266089
1101	74-75	0.08360861840954215
1101	76-77	0.09982662614727644
1101	78-79	0.09647532592183694
1101	80-81	0.0890691649055313
1101	82-83	0.0482912184875417
1101	84-85	0.08001273522055641
1101	86-87	0.05711965711957845
1101	88-89	0.09806352535441931
1101	90-91	-0.01602010804609222
1101	92-93	-0.06503844444243612
1101	94-95	-0.039648128030805196
1101	96-97	-0.02658907308305203
1101	98-99	-0.03318766188037614
1101	100	-0.025754998912915994
1102	1	0.0434187695228303
1102	2	0.106990490671528
1102	3	0.0656558650493082
1102	4	0.045955267644757214
1102	5	0.06947207923952448
1102	6	0.05199690619360808
1102	7	0.07597774793907064
1102	8	0.07469070161182145
1102	9	0.11531976800961274
1102	10-11	0.0898819713202812
1102	12-13	0.08016639125974478
1102	14-15	0.18840695233507887
1102	16-17	0.161709849112178
1102	18-19	0.15727703444545682
1102	20-21	0.16751279465992042
1102	22-23	0.1555653058279276
1102	24-25	0.18476451003267158
1102	26-27	0.15829650730002243
1102	28-29	0.1559784915419371
1102	30-31	0.15650048084030033
1102	32-33	0.1714871300298384
1102	34-35	0.15673843866570536
1102	36-37	0.17533774941163927
1102	38-39	0.143732869168943
1102	40-41	0.17329529043608716
1102	42-43	0.18628328523953996
1102	44-45	0.19515371482290078
1102	46-47	0.23500008474528755
1102	48-49	0.10954455642700367
1102	50-51	0.07004111747496466
1102	52-53	0.01244829032803807
1102	54-55	0.0164404001258589
1102	56-57	0.03609320256556714
1102	58-59	0.011950807844563371
1102	60-61	0.030827373119777235
1102	62-63	0.036221366673636624
1102	64-65	-0.014435822472478321
1102	66-67	-0.0314022688464064
1102	68-69	0.011895842217640507
1102	70-71	0.024254992037128886
1102	72-73	-0.016603024546739675
1102	74-75	0.005573666010398881
1102	76-77	0.007246747457813285
1102	78-79	-0.051492782302439366
1102	80-81	-0.06676044712363449
1102	82-83	-0.07426854127938753
1102	84-85	0.02011466313471999
1102	86-87	0.027716379187914697
1102	88-89	0.005056791186873966
1102	90-91	-0.022450800248471126
1102	92-93	-0.07364968158702112
1102	94-95	-0.07292643780250785
1102	96-97	-0.10468671055240719
1102	98-99	-0.009123559928582381
1102	100	0.015941517787183557
1103	1	0.1206541921755715
1103	2	0.10911226153652365
1103	3	0.08173176226755885
1103	4	0.053431050924466206
1103	5	0.07154988053147093
1103	6	0.08143501328437708
1103	7	0.07542621655530724
1103	8	0.06618712956193917
1103	9	0.14216725476616432
1103	10-11	0.03937634512665511
1103	12-13	0.055882880231983734
1103	14-15	0.1277192634331925
1103	16-17	0.14108322056521416
1103	18-19	0.08796688837088595
1103	20-21	0.08454268823444266
1103	22-23	0.13824738765339362
1103	24-25	0.1397285553480927
1103	26-27	0.09178559806660047
1103	28-29	0.1032674567084797
1103	30-31	0.1646662757623929
1103	32-33	0.17556220138479972
1103	34-35	0.21883900854961524
1103	36-37	0.1902134445610315
1103	38-39	0.15799932286994078
1103	40-41	0.09848070343464599
1103	42-43	0.08459504480626379
1103	44-45	0.11444493486384744
1103	46-47	0.20521989915545902
1103	48-49	0.2045122626873166
1103	50-51	0.18026793834101085
1103	52-53	0.18435988185453311
1103	54-55	0.20174299681590213
1103	56-57	0.1952702259784047
1103	58-59	0.2147707390608602
1103	60-61	0.2488580285149382
1103	62-63	0.20323895354700738
1103	64-65	0.23673904929886902
1103	66-67	0.14261890632646157
1103	68-69	0.2294574091785151
1103	70-71	0.19857965285009982
1103	72-73	0.17944439759367015
1103	74-75	0.22446304238803094
1103	76-77	0.21782491866768083
1103	78-79	0.09183739191265516
1103	80-81	0.1709854240378661
1103	82-83	0.24578287444527547
1103	84-85	0.20573484440405565
1103	86-87	0.22415295594635865
1103	88-89	0.1909664786396803
1103	90-91	0.14703601270305455
1103	92-93	0.12958485402737807
1103	94-95	0.11237221596387315
1103	96-97	0.11634689940689213
1103	98-99	0.1429274055858869
1103	100	0.13325329455939539
1104	1	0.03878639858946542
1104	2	0.11176505484574761
1104	3	0.09626476966168696
1104	4	0.08276767343298275
1104	5	-0.021907741554962
1104	6	0.03899311250596327
1104	7	0.06422217274990061
1104	8	0.07596577105069002
1104	9	0.04791293376817407
1104	10-11	0.0857183244115447
1104	12-13	0.14017509096442637
1104	14-15	0.19642826217531706
1104	16-17	0.18313514839508827
1104	18-19	0.14004363393028996
1104	20-21	0.14465727419997876
1104	22-23	0.18022439227954834
1104	24-25	0.14699959631992243
1104	26-27	0.14139185568980395
1104	28-29	0.1312309137302492
1104	30-31	0.09017643995588998
1104	32-33	0.12445899211219569
1104	34-35	0.18645729297577418
1104	36-37	0.15027239655758962
1104	38-39	0.16372094268167814
1104	40-41	0.19465778186713578
1104	42-43	0.1205713290528081
1104	44-45	0.19067848026242729
1104	46-47	0.16491807963846838
1104	48-49	0.17585879088100853
1104	50-51	0.16380751989724018
1104	52-53	0.14692515462385813
1104	54-55	0.1108618487504387
1104	56-57	0.1947584446536439
1104	58-59	0.19638197884442832
1104	60-61	0.1797997759567309
1104	62-63	0.12429700413831313
1104	64-65	0.14293345314052175
1104	66-67	0.16935439065377977
1104	68-69	0.25847328126366875
1104	70-71	0.23743920069554747
1104	72-73	0.3077848207096565
1104	74-75	0.2758241782796489
1104	76-77	0.20758161370743977
1104	78-79	0.24816756497595094
1104	80-81	0.20464979228401603
1104	82-83	0.2364092138138858
1104	84-85	0.17450873780928333
1104	86-87	0.20227165756912058
1104	88-89	0.18920275850251755
1104	90-91	0.16836097139059092
1104	92-93	0.21424470035569954
1104	94-95	0.21660993751666524
1104	96-97	0.23261340170787292
1104	98-99	0.17483491777718996
1104	100	0.13008930162730792
1105	1	0.016277476878734376
1105	2	0.05111556774610193
1105	3	0.026112008467826797
1105	4	0.019414006290638497
1105	5	0.019127199291801844
1105	6	-0.008201785149189789
1105	7	-0.031032126809002136
1105	8	-0.08898002661633342
1105	9	-0.014245347349167048
1105	10-11	-0.022894346769930962
1105	12-13	-0.03643098498380226
1105	14-15	-0.057855391100005704
1105	16-17	-0.07552165529143195
1105	18-19	-0.08760728467548518
1105	20-21	-0.054134346448662995
1105	22-23	0.05942398097356261
1105	24-25	-0.0407415362948953
1105	26-27	-0.06972113472426145
1105	28-29	0.014705978864895997
1105	30-31	0.030921271188262267
1105	32-33	-0.02313858664864199
1105	34-35	8.257837113134769E-4
1105	36-37	-0.034948254766497655
1105	38-39	-0.06586878183739486
1105	40-41	0.04734359203463612
1105	42-43	-0.008051848801187589
1105	44-45	0.09046689066335745
1105	46-47	0.028954792506119986
1105	48-49	-0.10303343843776247
1105	50-51	-0.1382195510261539
1105	52-53	-0.10644291718891452
1105	54-55	-0.1494783747795836
1105	56-57	-0.21796022966863404
1105	58-59	-0.15568362401231184
1105	60-61	-0.1410315505050761
1105	62-63	-0.11822813682807265
1105	64-65	-0.12943971071204885
1105	66-67	-0.09840457418135173
1105	68-69	-0.1311208565059161
1105	70-71	-0.1795480053584413
1105	72-73	-0.1806315168300756
1105	74-75	-0.09865715277462783
1105	76-77	-0.18580388708448226
1105	78-79	-0.18550464028276537
1105	80-81	-0.1226581473104531
1105	82-83	-0.07054434400529885
1105	84-85	-0.0592498282651448
1105	86-87	-0.0682194901672375
1105	88-89	-0.050707811568479144
1105	90-91	-0.08125214111593237
1105	92-93	-0.03242743993638442
1105	94-95	-0.11257923411397286
1105	96-97	-0.13120634501187567
1105	98-99	-0.028640797023768982
1105	100	-0.030959394223565084
1106	1	0.07154812169068947
1106	2	0.07930575169581999
1106	3	0.08294941643628562
1106	4	0.03362673236783564
1106	5	-0.0020892636521097074
1106	6	0.004999795110308014
1106	7	-0.0023155855863166153
1106	8	-0.008754000705522458
1106	9	0.046615157716281885
1106	10-11	0.04514545555727523
1106	12-13	0.07241165524586535
1106	14-15	0.06809553404713142
1106	16-17	0.07498062451038123
1106	18-19	0.06645182065751953
1106	20-21	0.01505739551858909
1106	22-23	0.12380044465167117
1106	24-25	0.07330259349887314
1106	26-27	0.010246841159400333
1106	28-29	-0.04138886045847556
1106	30-31	0.06098814825387677
1106	32-33	0.0567327014528729
1106	34-35	0.08193662969431159
1106	36-37	0.0672696798183594
1106	38-39	-0.0035518057086463273
1106	40-41	0.035089400881112454
1106	42-43	-0.0020783145847289575
1106	44-45	0.06904145038574683
1106	46-47	0.07088689619688182
1106	48-49	0.00915138152645767
1106	50-51	-0.020919078222355836
1106	52-53	-0.02836221555197227
1106	54-55	-0.05014039726555808
1106	56-57	0.00797352813360419
1106	58-59	-0.054723278419622545
1106	60-61	-0.08918606092802861
1106	62-63	-0.048074807604827186
1106	64-65	-0.09545619568197594
1106	66-67	-0.03134617173775922
1106	68-69	0.02288890620682338
1106	70-71	-0.057547027143698415
1106	72-73	-0.06372729657843479
1106	74-75	-0.060593802032812505
1106	76-77	-0.10408581375725134
1106	78-79	-0.03127537309085682
1106	80-81	-0.04724000369427728
1106	82-83	-0.010693581138156105
1106	84-85	-0.00998748424235174
1106	86-87	0.018685982544781155
1106	88-89	0.039776512702147215
1106	90-91	-0.027060609691595516
1106	92-93	-0.04137713313757985
1106	94-95	-0.15171586782437885
1106	96-97	-0.07152223495515386
1106	98-99	-0.060727104500635676
1106	100	-0.07413839780495834
1107	1	-0.008936777433227405
1107	2	-0.023586509384387
1107	3	-0.020406538594670565
1107	4	-0.03447078018954386
1107	5	-0.08166531013687717
1107	6	-0.1124500401913906
1107	7	-0.06344592211483047
1107	8	-0.026299396738998837
1107	9	-0.030616282212236
1107	10-11	0.01706505375443612
1107	12-13	-0.07052416578347476
1107	14-15	-0.040602689702019745
1107	16-17	0.010002034300349294
1107	18-19	0.01791249825464547
1107	20-21	-0.04298822559336912
1107	22-23	-0.01017154648430818
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2216	2	-0.08221913353970933
2216	3	-0.09963671234115168
2216	4	-0.06358744311719278
2216	5	-0.04651698507586133
2216	6	-0.09117991718520813
2216	7	-0.10788749008432319
2216	8	-0.075775865304891
2216	9	-0.12230215219768326
2216	10-11	-0.11186467028447566
2216	12-13	-0.09739723821792978
2216	14-15	-0.184427121864573
2216	16-17	-0.150895526031114
2216	18-19	-0.18248100711667803
2216	20-21	-0.1344390951713379
2216	22-23	-0.20555929580736887
2216	24-25	-0.19919305761350614
2216	26-27	-0.24803607220572133
2216	28-29	-0.22273948180243508
2216	30-31	-0.15846708616818006
2216	32-33	-0.1829025029224951
2216	34-35	-0.14463544323013622
2216	36-37	-0.13241123144352684
2216	38-39	-0.1723822564802191
2216	40-41	-0.2157204785069382
2216	42-43	-0.23319338524810718
2216	44-45	-0.08184195818596862
2216	46-47	-0.13218708717251104
2216	48-49	-0.12892085097429629
2216	50-51	-0.0533233319846218
2216	52-53	-0.05454609562650603
2216	54-55	-0.030593075729619557
2216	56-57	-0.01085122934964744
2216	58-59	-0.04193924583065467
2216	60-61	-0.060952140863619775
2216	62-63	-0.03496236169335276
2216	64-65	-0.03378392362017024
2216	66-67	-0.05649467623682369
2216	68-69	-0.0723948430863004
2216	70-71	-0.036132644620046506
2216	72-73	-0.05301460819126902
2216	74-75	-0.056130090632905194
2216	76-77	-0.07169109442814658
2216	78-79	-0.12906298314512554
2216	80-81	-0.08937138654109589
2216	82-83	-0.10843283473710841
2216	84-85	-0.06436062308905832
2216	86-87	-0.05405968968933195
2216	88-89	-0.08358810391236204
2216	90-91	-0.04885573087195638
2216	92-93	0.010299312177572517
2216	94-95	-0.04566742414495195
2216	96-97	-0.014222344459710712
2216	98-99	0.05577965436324206
2216	100	0.035690608294707715
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	12.0
3	11.0
4	9.0
5	22.0
6	33.0
7	114.0
8	213.0
9	420.0
10	789.0
11	1359.0
12	2126.0
13	3310.0
14	3889.0
15	5215.0
16	6588.0
17	9154.0
18	10782.0
19	6108.0
20	3936.0
21	3602.0
22	3676.0
23	3940.0
24	4445.0
25	5426.0
26	6714.0
27	8681.0
28	10996.0
29	14266.0
30	18483.0
31	23349.0
32	29907.0
33	39247.0
34	54076.0
35	83212.0
36	151894.0
37	363017.0
38	728986.0
39	407018.0
40	1953.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.48334616942047	20.35767028988384	13.847739525355903	23.311244015339785
2	15.47358400761542	20.543094558146915	40.700311359669996	23.283010074567667
3	17.922803322594497	25.903653882194057	30.583228969279787	25.590313825931666
4	11.913391050157315	17.204535110517043	39.80641297309732	31.075660866228322
5	12.04902340068843	38.033260919163965	36.29383289190449	13.623882788243113
6	29.968229666589984	39.14330165554772	17.778793599923848	13.10967507793846
7	26.280211057199832	32.96449887797772	23.100866976648252	17.65442308817419
8	24.627610100685583	36.48637660810363	20.736232332427022	18.14978095878376
9	24.664906605188463	16.47244016410714	21.944942302209995	36.9177109284944
10-11	23.360853953140165	26.976346975479338	29.446236872342084	20.21656219903841
12-13	24.45948840294738	24.376245055721977	29.659644279709546	21.504622261621098
14-15	21.506665668473165	26.34841646682139	27.220141826109074	24.924776038596374
16-17	20.439067753837673	29.877841999268213	27.707268001931602	21.975822244962515
18-19	20.180061636826615	28.224133554390335	30.73876436804404	20.85704044073901
20-21	21.497904290478132	27.600077938381084	30.287935713726178	20.614082057414606
22-23	20.984479720611173	27.47135064705842	30.436609495214118	21.107560137116295
24-25	20.95867254774659	27.523027660461093	30.417211107814097	21.101088683978222
26-27	20.681251394432188	27.444185527653143	30.515729195071767	21.358833882842905
28-29	20.687113589405786	27.46490850104547	30.617379322381655	21.23059858716709
30-31	21.021249644450542	28.04199686992482	30.048374565498097	20.888378920126545
32-33	20.606055646846425	27.701079397762946	30.62493289563309	21.06793205975754
34-35	20.760715932929642	27.381310163212476	30.617762436830176	21.24021146702771
36-37	21.212100173051606	27.91261178987533	29.86515166129791	21.010136375775154
38-39	21.025657683320176	28.054765947655113	29.973438758385935	20.94613761063878
40-41	20.825848686983907	27.78215587571602	30.181764078656983	21.210231358643092
42-43	20.900694988047416	27.68447160799391	30.01324813991702	21.401585264041653
44-45	21.09321981156239	27.88977299860974	29.823540890425853	21.193466299402015
46-47	21.147167127993708	28.231008317771188	29.443987690452367	21.177836863782737
48-49	21.088438072622868	28.102058671786427	29.250459213942747	21.559044041647958
50-51	21.247749554621304	28.05955207769844	29.095587466029492	21.597110901650765
52-53	21.240493299756505	28.101952186237366	29.015225099126518	21.64232941487961
54-55	21.312085826955336	28.52949000084536	28.8726411457609	21.285783026438406
56-57	21.156279407375383	28.205769572111222	28.933064475345155	21.704886545168243
58-59	20.91494939919602	27.824591812248507	29.392021870494467	21.868436918061008
60-61	21.26516137092904	28.244250776412912	28.953186168546058	21.53740168411199
62-63	21.0628750085525	28.186041922621936	29.049447830738828	21.701635238086737
64-65	21.105766072404684	27.857248215416767	29.25625735183838	21.78072836034017
66-67	20.95082699523865	28.025843709662585	29.029799890479314	21.99352940461945
68-69	21.54392862543475	28.15440525740407	28.821942869608545	21.479723247552634
70-71	21.47398601766112	27.92679921843277	28.93928178513049	21.659932978775622
72-73	21.200385925805467	28.103742845697106	29.0660288115627	21.629842416934725
74-75	21.289315480477022	28.095714883145234	28.891537526968058	21.723432109409686
76-77	21.298213361415034	28.17270145544906	28.71502430375831	21.8140608793776
78-79	21.46742377330034	28.288845138045698	28.783372343859153	21.460358744794807
80-81	21.33979222216383	28.027077356935827	28.891372271763178	21.741758149137166
82-83	21.152980420076908	28.118672420529073	29.00768184261097	21.720665316783048
84-85	21.117061177379348	28.037884530813777	28.86615623129931	21.97889806050756
86-87	21.361439442372998	28.170347136095604	28.700770039100625	21.767443382430773
88-89	21.09646213295336	28.072046398126304	28.736481012683328	22.095010456237006
90-91	21.001895162439837	27.93243851257637	28.898537539461895	22.1671287855219
92-93	21.1446582820086	28.142579676768833	28.698361781427906	22.014400259794666
94-95	20.919117610603585	27.895990932970015	28.88016131063403	22.304730145792366
96-97	20.8556811229473	27.79839442968639	28.971064632336095	22.37485981503021
98-99	20.879156837605567	27.9387033472849	28.820021834645694	22.362117980463843
100	20.9923955541409	27.900502633147212	28.71662457399139	22.390477238720504
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	25.0
1	25.5
2	117.0
3	214.5
4	145.0
5	94.5
6	250.0
7	596.0
8	990.5
9	1193.5
10	1014.0
11	779.0
12	793.0
13	901.5
14	1025.0
15	1138.0
16	1310.5
17	1558.0
18	1849.0
19	2236.0
20	2734.5
21	3482.5
22	4606.5
23	6382.5
24	8568.5
25	11122.0
26	14762.0
27	19298.0
28	24979.5
29	31081.5
30	36866.5
31	43399.0
32	49856.5
33	55884.0
34	61424.0
35	65874.5
36	69460.5
37	72729.5
38	75134.0
39	75737.0
40	75976.0
41	75798.5
42	73948.0
43	72376.5
44	70892.5
45	68173.5
46	66123.5
47	64339.5
48	62015.5
49	59979.0
50	58245.0
51	55614.5
52	51615.0
53	48455.0
54	46359.5
55	44009.5
56	40665.0
57	36747.0
58	33312.5
59	30081.0
60	26727.5
61	22651.5
62	18736.0
63	15459.5
64	12605.5
65	9925.5
66	7520.0
67	5463.5
68	3807.0
69	2880.0
70	2020.0
71	1403.5
72	1050.5
73	735.5
74	547.5
75	403.0
76	261.5
77	174.0
78	114.0
79	67.0
80	41.5
81	21.0
82	3.5
83	2.5
84	2.5
85	1.0
86	0.5
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09053147828087366
2	8.924242108738915E-4
3	0.0
4	1.9831649130530924E-4
5	0.03956414001540919
6	9.915824565265462E-5
7	0.004164646317411494
8	2.9747473695796383E-4
9	1.4873736847898191E-4
10-11	2.7268517554480015E-4
12-13	0.0
14-15	4.957912282632731E-5
16-17	0.0
18-19	9.915824565265462E-5
20-21	0.0
22-23	1.9831649130530924E-4
24-25	7.436868423949096E-5
26-27	0.0013882154391371645
28-29	0.008874662985912588
30-31	0.03631670747028475
32-33	0.02625214553654031
34-35	0.029152524221880458
36-37	0.026152987290887657
38-39	0.025087036150121617
40-41	0.031135689134933548
42-43	0.023227819044134342
44-45	0.0222362365876078
46-47	0.016460268778340666
48-49	0.010733880091899862
50-51	0.007684764038080733
52-53	0.005503282633722331
54-55	0.0040902776331720025
56-57	0.00257811438696902
58-59	6.941077195685823E-4
60-61	4.957912282632731E-4
62-63	0.0011898989478318553
64-65	0.002503745702729529
66-67	3.718434211974548E-4
68-69	6.44528596742255E-4
70-71	2.4789561413163653E-4
72-73	4.957912282632731E-4
74-75	4.214225440237821E-4
76-77	2.9747473695796383E-4
78-79	4.957912282632731E-5
80-81	7.93265965221237E-4
82-83	6.941077195685823E-4
84-85	3.470538597842911E-4
86-87	1.2394780706581827E-4
88-89	0.0
90-91	2.4789561413163655E-5
92-93	2.4789561413163655E-5
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2016978.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.73122620281283
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.73787897479352	61.00365270231454
2	11.684604798016165	17.230404789060046
3	2.9376875987764244	6.497979265757479
4	1.048142856283215	3.091234321179203
5	0.4933782139507622	1.8188690348171714
6	0.2890905067534114	1.2788998527912943
7	0.17336566816205257	0.8947724306540662
8	0.11776667110828941	0.694646485331004
9	0.089573376256474	0.5943919379864174
>10	0.40929008960786506	5.1058975679316605
>50	0.014435922118760566	0.7019400276157862
>100	0.004448670795248319	0.5540386078994032
>500	1.346613511169565E-4	0.0747138177133347
>1k	2.0199202667526826E-4	0.4585591589486318
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3920	0.19435016147920306	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3430	0.17005639129430267	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	4.957912282632731E-5	0.0
10-11	0.0	0.0	0.0	4.957912282632731E-5	0.0
12-13	0.0	0.0	0.0	4.957912282632731E-5	0.0
14-15	0.0	0.0	0.0	7.436868423949097E-5	0.0
16-17	0.0	0.0	0.0	9.915824565265462E-5	0.0
18-19	0.0	0.0	0.0	9.915824565265462E-5	0.0
20-21	0.0	0.0	0.0	1.4873736847898194E-4	0.0
22-23	0.0	0.0	0.0	2.2310605271847289E-4	0.0
24-25	0.0	0.0	0.0	2.4789561413163653E-4	0.0
26-27	0.0	0.0	0.0	4.4621210543694577E-4	0.0
28-29	0.0	0.0	0.0	0.0011155302635923645	0.0
30-31	0.0	0.0	0.0	0.0046604375456747674	0.0
32-33	0.0	0.0	0.0	0.011427987811468445	0.0
34-35	0.0	0.0	0.0	0.02179002448217085	0.0
36-37	0.0	0.0	0.0	0.0400351416822593	0.0
38-39	0.0	0.0	0.0	0.07171620116828245	0.0
40-41	0.0	0.0	0.0	0.11006565267444662	0.0
42-43	0.0	0.0	0.0	0.150720533392035	0.0
44-45	0.0	0.0	0.0	0.19593669340964553	0.0
46-47	0.0	0.0	0.0	0.24925904000936056	0.0
48-49	0.0	0.0	0.0	0.3126707381042332	0.0
50-51	0.0	0.0	0.0	0.384287781026863	0.0
52-53	0.0	0.0	0.0	0.45387207991361334	0.0
54-55	0.0	0.0	0.0	0.530000823013439	0.0
56-57	0.0	0.0	0.0	0.6202596161187678	0.0
58-59	0.0	2.4789561413163655E-5	0.0	0.725863147738845	0.0
60-61	0.0	4.957912282632731E-5	0.0	0.8357800630448126	0.0
62-63	0.0	4.957912282632731E-5	0.0	0.947481826772528	0.0
64-65	0.0	7.436868423949097E-5	0.0	1.0682069908546352	0.0
66-67	0.0	9.915824565265462E-5	0.0	1.2061609001188907	0.0
68-69	0.0	9.915824565265462E-5	0.0	1.366623731146299	0.0
70-71	2.4789561413163655E-5	9.915824565265462E-5	0.0	1.5344738514748302	0.0
72-73	4.957912282632731E-5	9.915824565265462E-5	0.0	1.7128099562811294	0.0
74-75	4.957912282632731E-5	9.915824565265462E-5	0.0	1.8906254802977522	0.0
76-77	4.957912282632731E-5	9.915824565265462E-5	0.0	2.0790757261606228	0.0
78-79	4.957912282632731E-5	9.915824565265462E-5	0.0	2.301710777212245	0.0
80-81	4.957912282632731E-5	9.915824565265462E-5	0.0	2.5441278982715727	0.0
82-83	4.957912282632731E-5	9.915824565265462E-5	0.0	2.7978738488967156	0.0
84-85	4.957912282632731E-5	9.915824565265462E-5	0.0	3.0498597406615247	0.0
86-87	4.957912282632731E-5	1.4873736847898191E-4	0.0	3.3110425597106166	0.0
88	4.957912282632731E-5	1.4873736847898191E-4	0.0	3.520563932774676	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2875	0.0	54.17612	1
GTATCAA	5320	0.0	43.164425	1
TCAACGC	6245	0.0	36.057915	4
ATCAACG	6295	0.0	35.77151	3
CAACGCA	6430	0.0	35.020477	5
AACGCAG	6715	0.0	33.59414	6
TATCAAC	6835	0.0	33.290104	2
ACGCAGA	7660	0.0	29.26563	7
CGCAGAG	7725	0.0	28.957829	8
GCAGAGT	8680	0.0	25.446947	9
GTACATG	5070	0.0	23.945807	1
GTGTACG	120	1.823857E-4	23.528147	1
TATAACG	160	2.0355674E-6	23.505976	2
TACATGG	5090	0.0	22.53618	2
GTGGTAT	1420	0.0	22.20262	1
CAGAGTA	8575	0.0	21.346706	10-11
GAGTACT	6155	0.0	21.34083	12-13
AGAGTAC	8125	0.0	20.909443	10-11
ACATGGG	5320	0.0	20.677677	3
TGGTATC	1395	0.0	19.546188	2
>>END_MODULE