FastQCFastQC Report
Wed 25 May 2016
SRR1033130_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033130_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2369819
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA27300.035.5486071
TCAACGC26150.034.8526654
CAACGCA26750.034.07025
ATCAACG27400.033.7806053
AACGCAG28600.032.020486
GTACATG60400.028.9370961
TATCAAC34000.027.790622
TACATGG62100.026.9488052
GGTATCA17550.026.8436051
ACATGGG62850.025.4918233
ACGCAGA35100.025.287977
CGCAGAG35800.024.5311458
CATGGGG42650.019.9372624
GAGTACA43950.019.723181
TCTGTCG5500.019.6392298
GCAGAGT45150.018.7229399
GTCGTAC1801.3143872E-418.320761
AATACCG3358.4946805E-1018.2303645
GGACCGT2854.8719812E-818.1262136
CTTAGAC7050.017.9939483
Produced by FastQC (version 0.11.5)