FastQCFastQC Report
Wed 25 May 2016
SRR1033130_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033130_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2369819
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39110.16503370088601704No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33620.14186737468135752No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33050.050.275781
GTATCAA56550.041.535891
ATCAACG66200.034.8703423
TCAACGC66400.034.7653124
CAACGCA68350.033.7046855
AACGCAG69500.033.200616
TATCAAC72850.031.8170032
ACGCAGA81200.028.3589047
CGCAGAG82850.027.906988
GTACATG60150.027.5462551
TACATGG62700.024.8950162
GCAGAGT94400.023.8951369
ACATGGG63400.023.50733
GTGGTAT16800.022.9752451
TATAACG1805.652644E-620.8958192
TGGTATC16250.020.8315222
GAGTACT65050.020.76972812-13
CAGAGTA93300.020.24811710-11
AGAGTAC87400.019.89438610-11
CATGGGG44050.019.7450644
Produced by FastQC (version 0.11.5)