##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033130_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2369819 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.76453433785449 34.0 31.0 34.0 31.0 34.0 2 32.869531386152275 34.0 31.0 34.0 31.0 34.0 3 32.96731733520577 34.0 31.0 34.0 31.0 34.0 4 36.381526606040374 37.0 37.0 37.0 35.0 37.0 5 36.31973623302033 37.0 37.0 37.0 35.0 37.0 6 36.4025940377725 37.0 37.0 37.0 35.0 37.0 7 36.37413912201733 37.0 37.0 37.0 35.0 37.0 8 36.37533161815312 37.0 37.0 37.0 35.0 37.0 9 38.19988404177703 39.0 39.0 39.0 37.0 39.0 10-11 38.18049289840279 39.0 39.0 39.0 37.0 39.0 12-13 38.1408383931431 39.0 39.0 39.0 37.0 39.0 14-15 39.685024046140235 41.0 40.0 41.0 37.0 41.0 16-17 39.698451231929525 41.0 40.0 41.0 37.0 41.0 18-19 39.71112772747624 41.0 40.0 41.0 37.0 41.0 20-21 39.70285937449231 41.0 40.0 41.0 37.0 41.0 22-23 39.6363471218688 41.0 40.0 41.0 37.0 41.0 24-25 39.588094491604636 41.0 40.0 41.0 37.0 41.0 26-27 39.49021317661813 41.0 39.5 41.0 36.5 41.0 28-29 39.4011873902606 41.0 39.0 41.0 36.0 41.0 30-31 39.314565584966616 41.0 39.0 41.0 36.0 41.0 32-33 39.35464839297853 41.0 39.0 41.0 36.0 41.0 34-35 39.30004527771952 41.0 39.0 41.0 35.5 41.0 36-37 39.219209146352526 41.0 39.0 41.0 35.0 41.0 38-39 39.132052279098104 41.0 39.0 41.0 35.0 41.0 40-41 39.02002114929452 41.0 39.0 41.0 35.0 41.0 42-43 38.912957487470564 41.0 39.0 41.0 35.0 41.0 44-45 38.775444031801584 41.0 39.0 41.0 35.0 41.0 46-47 38.5796119872446 41.0 39.0 41.0 35.0 41.0 48-49 38.40639348405933 41.0 39.0 41.0 35.0 41.0 50-51 38.2801055692439 41.0 38.0 41.0 34.5 41.0 52-53 38.14261046940716 40.0 38.0 41.0 34.0 41.0 54-55 38.00966339623406 40.0 38.0 41.0 34.0 41.0 56-57 37.85346560222531 40.0 37.0 41.0 34.0 41.0 58-59 37.66184421679462 40.0 37.0 41.0 34.0 41.0 60-61 37.47112479898254 40.0 36.5 41.0 33.0 41.0 62-63 37.236558150643575 39.5 36.0 41.0 33.0 41.0 64-65 36.987591668393236 39.0 35.5 41.0 33.0 41.0 66-67 36.710461220878045 39.0 35.0 41.0 33.0 41.0 68-69 36.39128916596584 38.0 35.0 40.5 32.0 41.0 70-71 36.05580848157602 37.0 35.0 40.0 32.0 41.0 72-73 35.7054715993078 37.0 35.0 39.5 32.0 41.0 74-75 35.3684211747817 36.0 35.0 39.0 31.0 41.0 76-77 35.02506161862995 36.0 35.0 39.0 31.0 40.5 78-79 34.68852051570183 35.5 35.0 37.5 31.0 39.5 80-81 34.34810105750692 35.0 35.0 37.0 31.0 39.0 82-83 34.02352837917158 35.0 34.5 37.0 31.0 39.0 84-85 33.751092172018204 35.0 34.0 36.0 30.5 37.5 86-87 33.52254961243875 35.0 34.0 36.0 30.0 37.0 88-89 33.30764628015895 35.0 34.0 36.0 30.0 37.0 90-91 33.11209147196473 35.0 34.0 35.0 30.0 36.0 92-93 32.94992571162608 35.0 34.0 35.0 30.0 36.0 94-95 32.80138630840583 35.0 34.0 35.0 30.0 36.0 96-97 32.679279936568996 35.0 34.0 35.0 30.0 36.0 98-99 32.57589777953506 35.0 34.0 35.0 30.0 36.0 100 32.48303773410543 35.0 34.0 35.0 29.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.18920348959376554 1101 2 0.1963213841516236 1101 3 0.19394318131595156 1101 4 0.1319938745343947 1101 5 0.1714971372196743 1101 6 0.17285426325635456 1101 7 0.16804558519628188 1101 8 0.13770967286976799 1101 9 0.17505406366480258 1101 10-11 0.16900781181403346 1101 12-13 0.20408811383894943 1101 14-15 0.2622872562122751 1101 16-17 0.31486873498910484 1101 18-19 0.29040452372007763 1101 20-21 0.2381437525631256 1101 22-23 0.3014716373726998 1101 24-25 0.29623167971648456 1101 26-27 0.26589249776564117 1101 28-29 0.22386095558300667 1101 30-31 0.3000311804502189 1101 32-33 0.27717287030899485 1101 34-35 0.2951767774751488 1101 36-37 0.34224212948206656 1101 38-39 0.3586497437952403 1101 40-41 0.4679194565311917 1101 42-43 0.35132886668043994 1101 44-45 0.314718012773632 1101 46-47 0.3877965367196907 1101 48-49 0.34248285398020784 1101 50-51 0.3282224275149801 1101 52-53 0.4005100890303197 1101 54-55 0.3638960879137585 1101 56-57 0.37105293102197834 1101 58-59 0.3287360435488935 1101 60-61 0.3485582685963493 1101 62-63 0.30537817966926184 1101 64-65 0.2895758696737829 1101 66-67 0.36520805857111327 1101 68-69 0.4467131973216212 1101 70-71 0.42928384305079703 1101 72-73 0.39245525689020155 1101 74-75 0.34471410095085986 1101 76-77 0.33231760787050746 1101 78-79 0.30992066167746657 1101 80-81 0.3142544875014295 1101 82-83 0.4077792871047734 1101 84-85 0.400002530303567 1101 86-87 0.2949607520271229 1101 88-89 0.3548012480682914 1101 90-91 0.4369288754634084 1101 92-93 0.46385258358153436 1101 94-95 0.43501646911262526 1101 96-97 0.4792608701973933 1101 98-99 0.4851403208523166 1101 100 0.5509531158023933 1102 1 0.17140035313726543 1102 2 0.1695980333795859 1102 3 0.10244766173072861 1102 4 0.05378295368722519 1102 5 0.09816706049009838 1102 6 0.06538680067868796 1102 7 0.05530967988443791 1102 8 0.12847644428683225 1102 9 0.1682753801671808 1102 10-11 0.13799507635179964 1102 12-13 0.18305878889746907 1102 14-15 0.2065272398480147 1102 16-17 0.17788294699991525 1102 18-19 0.166551258088667 1102 20-21 0.21366840915204222 1102 22-23 0.19619638235514714 1102 24-25 0.19203595930736128 1102 26-27 0.21002438947950708 1102 28-29 0.22428561560575844 1102 30-31 0.25355668478995597 1102 32-33 0.22197177502675203 1102 34-35 0.2667707096321834 1102 36-37 0.28902222712848413 1102 38-39 0.2410298559154569 1102 40-41 0.23937676456048962 1102 42-43 0.17783860898413195 1102 44-45 0.19158409842084012 1102 46-47 0.21947996824959404 1102 48-49 0.23382744179644988 1102 50-51 0.2558009783115409 1102 52-53 0.23388694528523502 1102 54-55 0.2496792032724855 1102 56-57 0.1953611427218931 1102 58-59 0.14011190140737284 1102 60-61 0.1961454279775694 1102 62-63 0.18848664303597928 1102 64-65 0.18558802544578157 1102 66-67 0.21349070777765888 1102 68-69 0.18883144083804382 1102 70-71 0.22519000227804042 1102 72-73 0.2007130643392543 1102 74-75 0.19424169956004533 1102 76-77 0.1640672237795826 1102 78-79 0.18579292010264226 1102 80-81 0.13635438400640965 1102 82-83 0.10671010015171589 1102 84-85 0.14270327943319927 1102 86-87 0.1282324701957549 1102 88-89 0.1886939640204517 1102 90-91 0.1352906597721173 1102 92-93 0.09994562091100789 1102 94-95 0.04501906110338183 1102 96-97 0.046097631641572434 1102 98-99 0.10283640846012787 1102 100 0.08957497795206137 1103 1 0.09052835956880045 1103 2 0.1171930677608728 1103 3 0.13152554929519766 1103 4 0.05484266310412522 1103 5 0.02233892452255759 1103 6 0.06693631138031009 1103 7 0.03468306472563398 1103 8 0.1467700822225595 1103 9 0.1377352732191568 1103 10-11 0.10730454455009664 1103 12-13 0.05891104672512881 1103 14-15 0.09683150936337626 1103 16-17 0.10602063881630386 1103 18-19 0.14633209222007082 1103 20-21 0.13803811554428336 1103 22-23 0.1777285210068129 1103 24-25 0.10840009989020416 1103 26-27 0.16832751810093072 1103 28-29 0.13772576645090595 1103 30-31 0.16366503738365168 1103 32-33 0.18614892682958128 1103 34-35 0.1779288929956806 1103 36-37 0.1826146492362497 1103 38-39 0.18916713821701592 1103 40-41 0.13251494851311918 1103 42-43 0.09514669920096708 1103 44-45 0.10074895040996523 1103 46-47 0.1051542056719228 1103 48-49 0.07074723292952001 1103 50-51 0.08101133961138629 1103 52-53 0.06717857875366917 1103 54-55 0.09112907255104119 1103 56-57 0.0783948177726046 1103 58-59 0.1275231821757643 1103 60-61 0.06857814720629563 1103 62-63 0.015475160815029199 1103 64-65 -0.015461482022836037 1103 66-67 -0.015619456021092049 1103 68-69 0.013259323481150886 1103 70-71 0.002900056441774268 1103 72-73 0.0014222254732842998 1103 74-75 0.05838021687210926 1103 76-77 0.008785847579858341 1103 78-79 0.012022308754481514 1103 80-81 0.005060338711189161 1103 82-83 -0.04257341055422614 1103 84-85 -0.01823775330571209 1103 86-87 -0.0021260672184553187 1103 88-89 0.012758671673559263 1103 90-91 0.027096483647696346 1103 92-93 0.035587402487287534 1103 94-95 0.07500165313106066 1103 96-97 0.06956543551413574 1103 98-99 0.015479083066104238 1103 100 -0.0013777259593865665 1104 1 0.016873693117879895 1104 2 0.05607941285342832 1104 3 0.055769095033618044 1104 4 0.03175174399799374 1104 5 -0.021037775756646226 1104 6 -0.023305265115467932 1104 7 -0.010765471596904774 1104 8 0.05363420103812189 1104 9 0.054308068063321 1104 10-11 0.06126463295944973 1104 12-13 0.08981705325943068 1104 14-15 0.09011112460366633 1104 16-17 0.028769522510494028 1104 18-19 0.09712503943955397 1104 20-21 0.07953560062863829 1104 22-23 0.06346054908573251 1104 24-25 0.0865908237934434 1104 26-27 0.06149283464291244 1104 28-29 0.10103906882260816 1104 30-31 0.041044395451869775 1104 32-33 0.02810162713632991 1104 34-35 0.015555198221406386 1104 36-37 0.007494583962056822 1104 38-39 0.031730923431865676 1104 40-41 0.011725753871388633 1104 42-43 -0.036790061257192974 1104 44-45 -0.07212714222706751 1104 46-47 -4.10281860219186E-4 1104 48-49 -0.08642277261327536 1104 50-51 -0.038920184451590956 1104 52-53 -0.035182446767308306 1104 54-55 -0.09633120692993202 1104 56-57 -0.024252815177092657 1104 58-59 0.01312800131184133 1104 60-61 0.002115605850477209 1104 62-63 7.453010655211756E-5 1104 64-65 -0.014184142428320001 1104 66-67 -0.007109883505101777 1104 68-69 -0.02426550722250198 1104 70-71 0.006391847247613214 1104 72-73 0.05039319291536515 1104 74-75 0.03987140175713222 1104 76-77 -0.04705732909769722 1104 78-79 -0.015219512175264072 1104 80-81 0.03789238159386343 1104 82-83 0.0447341837680284 1104 84-85 0.09612773399577179 1104 86-87 0.07051755494860856 1104 88-89 0.019599121707472023 1104 90-91 0.009956843000040294 1104 92-93 0.007160421109027482 1104 94-95 -0.005668095928221817 1104 96-97 0.039215602314499165 1104 98-99 0.09879835857761776 1104 100 0.1790852870507038 1105 1 0.01784012073104435 1105 2 0.0566508224961737 1105 3 0.07324843319218388 1105 4 0.04851060912875482 1105 5 0.03282545030625528 1105 6 0.017244944401397788 1105 7 -0.05061177068430567 1105 8 -0.10572449515413496 1105 9 8.150160733251255E-4 1105 10-11 -0.025234779068306068 1105 12-13 -0.005920147992696911 1105 14-15 -0.014630747301197289 1105 16-17 -0.025723082289346166 1105 18-19 -0.019960522943478054 1105 20-21 0.02916045777623566 1105 22-23 0.11005486480198101 1105 24-25 0.054740533119840507 1105 26-27 0.013932505378456028 1105 28-29 0.0581017748199244 1105 30-31 0.05217197714688382 1105 32-33 0.022360616486587048 1105 34-35 0.06727853657872629 1105 36-37 0.009563252494778851 1105 38-39 0.013134023950271967 1105 40-41 0.004443593446055161 1105 42-43 0.016737358124359503 1105 44-45 0.040756903021950563 1105 46-47 -0.06112876737622486 1105 48-49 -0.055778653163820024 1105 50-51 -0.015089917959379306 1105 52-53 -0.03456963062870955 1105 54-55 -0.00881153884440522 1105 56-57 -0.068757345047743 1105 58-59 -0.052086815588630486 1105 60-61 -0.11485567460400148 1105 62-63 -0.09572146836969608 1105 64-65 -0.18975389581122926 1105 66-67 -0.15579729758662353 1105 68-69 -0.17109749622493808 1105 70-71 -0.16746409127202355 1105 72-73 -0.140889083448144 1105 74-75 -0.13146288116517013 1105 76-77 -0.13301058944517763 1105 78-79 -0.1410646187555784 1105 80-81 -0.08567495364968636 1105 82-83 -0.10958244342445056 1105 84-85 -0.0792271196736749 1105 86-87 -0.10136536404372265 1105 88-89 -0.1331001677570569 1105 90-91 -0.11661950229699869 1105 92-93 -0.09136964828825711 1105 94-95 -0.10877689563671566 1105 96-97 -0.09101486624140875 1105 98-99 -0.09597779790581029 1105 100 -0.07862625873660534 1106 1 0.0014883339996529799 1106 2 0.06781190281333238 1106 3 0.06980884950071697 1106 4 0.02498448725758351 1106 5 -0.04624948475446189 1106 6 -0.0059089302101327235 1106 7 -0.01294944228126127 1106 8 0.016343532854321552 1106 9 0.04483937414682515 1106 10-11 0.014917542070406853 1106 12-13 0.02394031671828145 1106 14-15 0.09170347671100387 1106 16-17 0.021561588237368312 1106 18-19 0.08236425981986173 1106 20-21 0.049648474805017884 1106 22-23 0.10260370096486326 1106 24-25 0.06278444863183097 1106 26-27 0.05931118121122125 1106 28-29 -0.03259038010275361 1106 30-31 0.03916102837187907 1106 32-33 0.030210446919596734 1106 34-35 -0.019181058472653945 1106 36-37 -0.009089274215689613 1106 38-39 0.014407182459159174 1106 40-41 -0.027197978151932034 1106 42-43 -0.014964617503309796 1106 44-45 -0.00828459395229686 1106 46-47 -0.03441156993308425 1106 48-49 0.00816604288706202 1106 50-51 -0.0916589373861143 1106 52-53 -0.05729303245691142 1106 54-55 -0.106863399961874 1106 56-57 -0.07464786680388613 1106 58-59 -0.1012131158596361 1106 60-61 -0.05092626770422726 1106 62-63 -0.09786115734241463 1106 64-65 -0.09018575491656122 1106 66-67 -0.01854472635542237 1106 68-69 -0.08112341648529053 1106 70-71 -0.013812598361674588 1106 72-73 0.006564995626078485 1106 74-75 0.007918644945611675 1106 76-77 0.001423272301281031 1106 78-79 -0.008471832224614673 1106 80-81 -0.03644408509123309 1106 82-83 -0.042268945322128104 1106 84-85 -0.03416610962323574 1106 86-87 -0.10474101848672746 1106 88-89 -0.04725205953204892 1106 90-91 -0.09510699331562478 1106 92-93 -0.10867296682624072 1106 94-95 -0.08189060651965718 1106 96-97 -0.07135190884426379 1106 98-99 -0.119879177335811 1106 100 -0.09810471648111019 1107 1 -0.029150033409308662 1107 2 -0.0025829127748764336 1107 3 -0.017271606928680683 1107 4 -0.00825556810921313 1107 5 -0.1763050143645728 1107 6 -0.19358690731389316 1107 7 -0.14918082450063253 1107 8 -0.06475606377362197 1107 9 -0.06491836142212293 1107 10-11 -0.06773984885798257 1107 12-13 -0.040740463397668236 1107 14-15 -0.008791271372160736 1107 16-17 0.02174233533945369 1107 18-19 0.028761249498735708 1107 20-21 0.034258213320192965 1107 22-23 -0.012405084640732866 1107 24-25 -0.008208912718558281 1107 26-27 0.018208626903422953 1107 28-29 -0.01317431233359656 1107 30-31 0.018295705012448593 1107 32-33 7.069120248601735E-4 1107 34-35 -0.023230645286886897 1107 36-37 0.003947581610837858 1107 38-39 -0.05987086929511065 1107 40-41 -0.07601748400533381 1107 42-43 -0.06652811762345578 1107 44-45 -0.05122759545164257 1107 46-47 -0.06549355163284787 1107 48-49 -0.007840628022663054 1107 50-51 0.006780170437750144 1107 52-53 0.006028526436338666 1107 54-55 -0.028744170791732415 1107 56-57 -0.07104242189919319 1107 58-59 -0.004563200836400938 1107 60-61 -0.033242372225799954 1107 62-63 -0.037913757914829205 1107 64-65 -0.03415502007028692 1107 66-67 0.004729741812433019 1107 68-69 0.015017685646931511 1107 70-71 -4.8295182703128603E-4 1107 72-73 -0.028547465552492213 1107 74-75 -0.03071186486372568 1107 76-77 -0.07674826649723343 1107 78-79 0.028514768370115462 1107 80-81 0.0035412365556979353 1107 82-83 0.037686510089713465 1107 84-85 0.0029562020462776673 1107 86-87 -0.06537165402006906 1107 88-89 -0.05682279948565849 1107 90-91 0.01212604894302416 1107 92-93 -0.013204427826622123 1107 94-95 -0.04816845838180228 1107 96-97 -0.009211349104113253 1107 98-99 -0.04489400342212235 1107 100 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2214 8 -0.05581071080908373 2214 9 -0.034929063749515876 2214 10-11 -0.06643541820801602 2214 12-13 -0.040246447426383725 2214 14-15 -0.07385823798159663 2214 16-17 -0.1542511924294061 2214 18-19 -0.0960082367543933 2214 20-21 -0.08293815275565208 2214 22-23 -0.11071983987298495 2214 24-25 -0.11414275567880594 2214 26-27 -0.17640054210871625 2214 28-29 -0.054727626174880584 2214 30-31 -0.06463289981019216 2214 32-33 -0.03955025208551888 2214 34-35 -0.09197616514067875 2214 36-37 -0.09962285325030251 2214 38-39 -0.07777830606066516 2214 40-41 0.014088379263924367 2214 42-43 -0.04713366439260369 2214 44-45 -0.0347511014994879 2214 46-47 -0.07341636319213052 2214 48-49 -0.18409042488664795 2214 50-51 -0.14566411351083985 2214 52-53 -0.10415102822462075 2214 54-55 -0.11995541845652014 2214 56-57 -0.19931541290766575 2214 58-59 -0.27508287886885796 2214 60-61 -0.3159450397768495 2214 62-63 -0.29447430758939674 2214 64-65 -0.1168134979332649 2214 66-67 -0.29374621756414854 2214 68-69 -0.22225051959872388 2214 70-71 -0.09998380506588944 2214 72-73 -0.1847010942444598 2214 74-75 -0.18895874089545828 2214 76-77 -0.14573285141430858 2214 78-79 -0.3413437272850004 2214 80-81 -0.19974089388344396 2214 82-83 -0.2067887649111526 2214 84-85 -0.25268932297210256 2214 86-87 -0.1617644924379107 2214 88-89 -0.21346111544010427 2214 90-91 -0.15904950491864156 2214 92-93 -0.15286087716862085 2214 94-95 -0.23303717100106525 2214 96-97 -0.2977997221259159 2214 98-99 -0.33489462804001136 2214 100 -0.42909570244491846 2215 1 -0.03778532806177992 2215 2 -0.07473210525113672 2215 3 -0.06156872322869589 2215 4 -0.0486546174656155 2215 5 -0.03005012939171081 2215 6 -0.023753851410191373 2215 7 -0.008732403417951673 2215 8 -0.013547830853255505 2215 9 -0.0636596202742794 2215 10-11 -0.034445848157055536 2215 12-13 -0.02776095900734532 2215 14-15 -0.09536894118525652 2215 16-17 -0.1097127932146833 2215 18-19 -0.0782882427495295 2215 20-21 -0.12346132567263624 2215 22-23 -0.09474730523720609 2215 24-25 -0.09944815362033665 2215 26-27 -0.052316954681238315 2215 28-29 0.012261641595294748 2215 30-31 0.012988839118008855 2215 32-33 -0.08316885835115073 2215 34-35 -0.11546254069072859 2215 36-37 -0.04704339456969109 2215 38-39 -0.0764490518552634 2215 40-41 -0.10674424744551914 2215 42-43 -0.006470769244607766 2215 44-45 -0.053942445716288034 2215 46-47 -0.06144925927400635 2215 48-49 -0.01067263041618105 2215 50-51 -0.03460089524092069 2215 52-53 -0.0389440151662086 2215 54-55 -0.07346430340820831 2215 56-57 -0.1614127679928714 2215 58-59 -0.08869303208975765 2215 60-61 -0.03346385780730543 2215 62-63 -0.06901959736369179 2215 64-65 -0.0701689588609824 2215 66-67 -0.03481309926082332 2215 68-69 -0.03174220230991409 2215 70-71 0.027014628482632475 2215 72-73 0.07921815459920367 2215 74-75 0.09405521846719012 2215 76-77 0.06278593188089587 2215 78-79 0.0017879605270962884 2215 80-81 -0.028939901489025033 2215 82-83 -0.03700203572264371 2215 84-85 -0.007421309290990052 2215 86-87 -0.09468961850942748 2215 88-89 -0.0833178800213048 2215 90-91 -0.12944744224591886 2215 92-93 -0.1091296217322224 2215 94-95 -0.06537812593671077 2215 96-97 -0.06822410751937014 2215 98-99 -0.07579375291611257 2215 100 -0.09211566896206591 2216 1 -0.08900842037230916 2216 2 -0.06805692734576496 2216 3 -0.13521437805928116 2216 4 -0.07262422278536462 2216 5 -0.07396322827910495 2216 6 -0.07122244061547889 2216 7 -0.04739197435139175 2216 8 -0.07077487383500625 2216 9 -0.160031534096845 2216 10-11 -0.08835475969706863 2216 12-13 -0.09502853278089418 2216 14-15 -0.21082241497678922 2216 16-17 -0.18424575605880023 2216 18-19 -0.1683122858609991 2216 20-21 -0.12912949204687152 2216 22-23 -0.1445885160178051 2216 24-25 -0.23309627762044016 2216 26-27 -0.22945739467132142 2216 28-29 -0.2797975888885986 2216 30-31 -0.20012768520160762 2216 32-33 -0.21488664934334878 2216 34-35 -0.1953480827360181 2216 36-37 -0.18764843213064353 2216 38-39 -0.2432107303443587 2216 40-41 -0.22087825166472186 2216 42-43 -0.1691566112314149 2216 44-45 -0.1258653712156459 2216 46-47 -0.22890020017550938 2216 48-49 -0.2680864541243153 2216 50-51 -0.18849683510696735 2216 52-53 -0.17973657306652768 2216 54-55 -0.11464174452932951 2216 56-57 -0.11640185585662266 2216 58-59 -0.12904546336833533 2216 60-61 -0.11657621753685987 2216 62-63 -0.14630596868582302 2216 64-65 -0.1200194432505981 2216 66-67 -0.08950628503567515 2216 68-69 -0.052316471970534906 2216 70-71 -0.024600174245506423 2216 72-73 -0.04988157709195917 2216 74-75 -0.02558491998836132 2216 76-77 -0.033493188958203746 2216 78-79 -0.025740150455817457 2216 80-81 -0.07889165596482428 2216 82-83 -0.1097808641301583 2216 84-85 -0.11780615837579944 2216 86-87 -0.15148643430482878 2216 88-89 -0.10416000337054498 2216 90-91 -0.17448099791829463 2216 92-93 -0.10082826243733933 2216 94-95 -0.11497771084314223 2216 96-97 -0.17771294391469894 2216 98-99 -0.0733067189007457 2216 100 -0.10534848320086354 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11.0 3 6.0 4 8.0 5 20.0 6 40.0 7 111.0 8 231.0 9 496.0 10 892.0 11 1426.0 12 2166.0 13 3398.0 14 4133.0 15 5440.0 16 6591.0 17 9364.0 18 11249.0 19 6410.0 20 4169.0 21 3957.0 22 4144.0 23 4438.0 24 5135.0 25 6163.0 26 7828.0 27 10119.0 28 12931.0 29 16778.0 30 21804.0 31 27843.0 32 35764.0 33 47332.0 34 66398.0 35 101469.0 36 193255.0 37 453951.0 38 852620.0 39 439829.0 40 1900.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.17076868949968 19.697776658995217 13.261951814729608 23.869502836775492 2 15.813057909968089 20.038805257192433 40.154917203354564 23.993219629484905 3 18.37435686016527 25.15073936026338 29.99520216522865 26.479701614342698 4 12.240628268981562 16.557846547385807 38.810826001882845 32.39069918174978 5 12.555854289377248 37.76503480326415 35.92957367742742 13.749537229931185 6 31.175334487570574 38.19882446718504 17.35360379843355 13.272237246810834 7 27.27844335382811 32.54166121592937 22.36201898122555 17.817876449016968 8 25.284157561865666 36.36149196645135 20.095053953646953 18.25929651803603 9 25.085692415393112 16.11456262582744 21.186102025771653 37.613642933007796 10-11 24.01240521130276 26.545582442140415 28.923093217791042 20.51891912876578 12-13 24.93437262508234 23.787512886005217 29.2160498333417 22.062064655570744 14-15 21.91942125525647 25.798199313576127 26.51430900720878 25.768070423958626 16-17 21.158620130904513 29.404355353721108 27.031958980833558 22.405065534540825 18-19 20.965360849364625 27.902727571752855 29.743438157816726 21.38847342106579 20-21 22.065630328729746 27.23625728378412 29.477061328312416 21.221051059173718 22-23 21.6440900146024 27.031007266177472 29.575614641730546 21.749288077489577 24-25 21.559212892975935 27.195216509288162 29.498811617350277 21.746758980385618 26-27 21.391057508544016 27.122880663958405 29.600761587433805 21.885300240063767 28-29 21.393059390560254 27.15845880709093 29.64444917094267 21.804032631406145 30-31 21.603631354072853 27.674532335045853 29.235958864859924 21.485877446021366 32-33 21.274634836813828 27.319214386577972 29.702802298094216 21.703348478513984 34-35 21.35426669565089 27.11924967445512 29.737305860229917 21.78917776966407 36-37 21.772588252276954 27.5433969813952 29.081781245277888 21.602233521049953 38-39 21.634501542938256 27.688597874117683 29.160667758183102 21.516232824760962 40-41 21.472828345484174 27.391870252956842 29.331051959643577 21.804249441915406 42-43 21.451684622139457 27.345704245727692 29.232680261881395 21.96993087025146 44-45 21.658409573219835 27.508593342438786 29.08732456666087 21.745672517680507 46-47 21.734752252257007 27.78708957096279 28.748554784609887 21.72960339217031 48-49 21.683493626771718 27.726386391062814 28.593530319230087 21.996589662935385 50-51 21.72625101386794 27.670618887779487 28.551555132049707 22.05157496630287 52-53 21.695139126994594 27.735576948437295 28.49913754450745 22.070146380060663 54-55 21.837189022263566 28.054454454285487 28.333389950346525 21.774966573104415 56-57 21.679300503152113 27.723772085828923 28.411689704508113 22.18523770651085 58-59 21.530322243063218 27.364940849313967 28.79877795336727 22.305958954255537 60-61 21.807094213889798 27.754188161934877 28.397131493336264 22.041586130839065 62-63 21.604229242640475 27.728199930289065 28.478415350584395 22.189155476486064 64-65 21.613761285739272 27.507548793659414 28.594794513090942 22.28389540751037 66-67 21.533883097853092 27.573479224534537 28.43511220871782 22.45752546889456 68-69 21.984133230061413 27.687093675382542 28.33058801909279 21.99818507546326 70-71 21.939343992853424 27.47646274073432 28.39048251407868 22.193710752333576 72-73 21.73818740974984 27.66167806424334 28.406866902804538 22.193267623202274 74-75 21.822374339818822 27.737314984699157 28.238284685146958 22.202025990335066 76-77 21.769414507049415 27.734690574947784 28.14843743571469 22.34745748228811 78-79 21.897328337447224 27.817406959568387 28.21541245915032 22.069852243834067 80-81 21.834421749646808 27.558945803112834 28.313755061600034 22.29287738564033 82-83 21.69250670255711 27.65107839064362 28.404621562664765 22.251793344134505 84-85 21.67380964588082 27.562088851445655 28.243662623514677 22.520438879158853 86-87 21.75623598913416 27.739135480127647 28.20045889706464 22.304169633673553 88-89 21.50247402965122 27.638029587082215 28.23782248257039 22.62167390069617 90-91 21.44161642724613 27.520392907644002 28.359359934239702 22.678630730870164 92-93 21.64386816039537 27.678506248789464 28.126198667493174 22.551426923321994 94-95 21.356441145927178 27.441547223648726 28.343282757037564 22.858728873386532 96-97 21.3560824687455 27.41099636723311 28.373812514795432 22.859108649225952 98-99 21.331966702942292 27.464080590121014 28.278699765678304 22.925252941258385 100 21.487210626634354 27.373651743023412 28.169071140032216 22.97006649031002 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 53.0 1 39.0 2 103.0 3 198.0 4 141.5 5 95.5 6 241.0 7 575.0 8 1019.0 9 1215.0 10 981.5 11 757.0 12 811.5 13 909.5 14 1059.5 15 1247.0 16 1374.0 17 1609.5 18 1920.5 19 2273.5 20 2743.5 21 3459.5 22 4578.5 23 6226.0 24 8502.5 25 11105.0 26 14513.5 27 19270.0 28 25042.0 29 31458.5 30 37782.0 31 44454.0 32 51398.5 33 58179.0 34 64987.5 35 70652.5 36 74731.0 37 78788.0 38 82213.5 39 83235.0 40 83951.0 41 84650.5 42 83643.5 43 83268.0 44 82375.0 45 80481.0 46 79581.5 47 77435.5 48 75474.5 49 74433.0 50 72478.0 51 69996.5 52 66253.5 53 62716.0 54 60497.5 55 57855.5 56 54124.5 57 50010.0 58 45506.5 59 40886.0 60 36424.5 61 31585.5 62 27154.0 63 22747.5 64 18340.5 65 14744.0 66 11615.0 67 8693.0 68 6517.5 69 4930.0 70 3483.0 71 2384.5 72 1757.5 73 1312.0 74 936.5 75 643.5 76 375.0 77 247.0 78 173.5 79 109.0 80 59.0 81 24.5 82 11.0 83 6.0 84 3.0 85 1.5 86 1.0 87 1.5 88 1.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09139938535390256 2 0.001561300673173774 3 0.0 4 2.5318389294709846E-4 5 0.03932789803778263 6 0.0 7 0.004599507388538956 8 2.953812084382816E-4 9 2.5318389294709846E-4 10-11 3.586771816750562E-4 12-13 0.0 14-15 2.109865774559154E-5 16-17 0.0 18-19 2.109865774559154E-5 20-21 0.0 22-23 1.476906042191408E-4 24-25 6.329597323677461E-5 26-27 0.0013503140957178586 28-29 0.008439463098236616 30-31 0.03778769602235445 32-33 0.0269429859411204 34-35 0.03012888326070472 36-37 0.02721726849181309 38-39 0.026921887283374805 40-41 0.03285061010988603 42-43 0.024411147011649415 44-45 0.022913142311712414 46-47 0.015296526865553868 48-49 0.010908006054470827 50-51 0.007637714103904138 52-53 0.005548946987090575 54-55 0.004029843629407984 56-57 0.00246854295623421 58-59 0.0010127355717883938 60-61 6.118610746221547E-4 62-63 0.0013925114112090418 64-65 0.0029327134266372242 66-67 6.962557056045209E-4 68-69 9.916369140428023E-4 70-71 5.274664436397885E-4 72-73 7.173543633501124E-4 74-75 6.329597323677462E-4 76-77 5.274664436397885E-4 78-79 1.0549328872795771E-4 80-81 6.329597323677462E-4 82-83 6.118610746221547E-4 84-85 4.0087449716623923E-4 86-87 2.7428255069269006E-4 88-89 4.219731549118308E-5 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2369819.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.90811392779696 #Duplication Level Percentage of deduplicated Percentage of total 1 78.9755831946848 53.63082901098862 2 13.355270987433693 18.138625275024975 3 3.739691347625506 7.618661582680482 4 1.5121634219178237 4.107526637321715 5 0.7592713286899443 2.5780341945393257 6 0.427737456020467 1.7428106336774343 7 0.27729389175437175 1.3181353634916653 8 0.1808866775988869 0.9826938488324712 9 0.13502408758835657 0.8252308010652256 >10 0.610279575568332 7.081048140934326 >50 0.02149413018267439 0.9610274663837265 >100 0.004992872571778188 0.5683648035997899 >500 6.220567266770301E-5 0.02120583493049758 >1k 2.488226906458639E-4 0.42580640652984764 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3911 0.16503370088601704 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3362 0.14186737468135752 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 4.219731549118308E-5 0.0 9 0.0 0.0 0.0 8.439463098236616E-5 0.0 10-11 0.0 0.0 0.0 1.2659194647354926E-4 0.0 12-13 0.0 0.0 0.0 1.2659194647354926E-4 0.0 14-15 0.0 0.0 0.0 1.2659194647354926E-4 0.0 16-17 0.0 0.0 0.0 1.2659194647354926E-4 0.0 18-19 0.0 0.0 0.0 1.4769060421914078E-4 0.0 20-21 0.0 0.0 0.0 1.8988791971032387E-4 0.0 22-23 0.0 0.0 0.0 3.3757852392946465E-4 0.0 24-25 0.0 0.0 0.0 5.06367785894197E-4 0.0 26-27 0.0 0.0 0.0 5.907624168765631E-4 0.0 28-29 0.0 0.0 0.0 0.001054932887279577 0.0 30-31 0.0 0.0 0.0 0.004219731549118308 0.0 32-33 0.0 0.0 0.0 0.012511504043135783 0.0 34-35 0.0 0.0 0.0 0.022892043653966822 0.0 36-37 0.0 0.0 0.0 0.04099469199968436 0.0 38-39 0.0 0.0 0.0 0.07458375513066609 0.0 40-41 0.0 0.0 0.0 0.11568394041907841 0.0 42-43 0.0 0.0 0.0 0.15695291496945546 0.0 44-45 0.0 0.0 0.0 0.20147108281265363 0.0 46-47 0.0 0.0 0.0 0.2600831540299069 0.0 48-49 0.0 0.0 0.0 0.32588986753840693 0.0 50-51 0.0 0.0 0.0 0.4015918515295894 0.0 52-53 0.0 0.0 0.0 0.47396024759696836 0.0 54-55 0.0 0.0 0.0 0.5528903262232263 0.0 56-57 0.0 0.0 0.0 0.6452813484911717 0.0 58-59 0.0 0.0 0.0 0.7535807586992931 0.0 60-61 0.0 0.0 0.0 0.8697921655620113 0.0 62-63 0.0 0.0 0.0 0.9875015771246665 0.0 64-65 0.0 0.0 0.0 1.1093041282899665 0.0 66-67 0.0 0.0 0.0 1.246150022427873 0.0 68-69 0.0 0.0 0.0 1.4051073098831597 0.0 70-71 0.0 0.0 0.0 1.5803527611180432 0.0 72-73 0.0 0.0 0.0 1.7670969808242738 0.0 74-75 0.0 0.0 0.0 1.9502966260292451 0.0 76-77 0.0 0.0 0.0 2.1470416095068865 0.0 78-79 0.0 0.0 0.0 2.3798441990717434 0.0 80-81 0.0 0.0 0.0 2.635559930948313 0.0 82-83 0.0 0.0 0.0 2.9042302386806753 0.0 84-85 0.0 0.0 0.0 3.161085298075507 0.0 86-87 0.0 0.0 0.0 3.4339542386992425 0.0 88 0.0 0.0 0.0 3.6624738007417443 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3305 0.0 50.27578 1 GTATCAA 5655 0.0 41.53589 1 ATCAACG 6620 0.0 34.870342 3 TCAACGC 6640 0.0 34.765312 4 CAACGCA 6835 0.0 33.704685 5 AACGCAG 6950 0.0 33.20061 6 TATCAAC 7285 0.0 31.817003 2 ACGCAGA 8120 0.0 28.358904 7 CGCAGAG 8285 0.0 27.90698 8 GTACATG 6015 0.0 27.546255 1 TACATGG 6270 0.0 24.895016 2 GCAGAGT 9440 0.0 23.895136 9 ACATGGG 6340 0.0 23.5073 3 GTGGTAT 1680 0.0 22.975245 1 TATAACG 180 5.652644E-6 20.895819 2 TGGTATC 1625 0.0 20.831522 2 GAGTACT 6505 0.0 20.769728 12-13 CAGAGTA 9330 0.0 20.248117 10-11 AGAGTAC 8740 0.0 19.894386 10-11 CATGGGG 4405 0.0 19.745064 4 >>END_MODULE