FastQCFastQC Report
Wed 25 May 2016
SRR1033129_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033129_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3309615
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA43700.034.3847921
GGTATCA34500.030.9930211
TCAACGC48400.030.4769424
CAACGCA49450.029.6389125
ATCAACG50050.029.382793
AACGCAG51950.028.2931866
GTACATG112500.027.2993451
TATCAAC56150.026.454942
TACATGG116350.025.372472
ACATGGG113000.024.9474163
ACGCAGA63150.022.9777857
CGCAGAG65150.022.0561688
CATGGGG84350.021.1077144
GAGTACA75650.019.8627281
ATATCCG10200.018.06124194
GCAGAGT77600.017.8518569
AGTACAT79200.017.5685562
CTAGACT9350.016.5801894
ATGGGGG42550.016.4498025
TAGACAG14650.016.0326885
Produced by FastQC (version 0.11.5)