##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033129_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3309615 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.737863769652964 33.0 31.0 34.0 30.0 34.0 2 31.778365157276603 34.0 31.0 34.0 30.0 34.0 3 31.957861563958346 34.0 31.0 34.0 30.0 34.0 4 35.34858223690671 37.0 35.0 37.0 33.0 37.0 5 35.40693434130556 37.0 35.0 37.0 35.0 37.0 6 35.49200133550277 37.0 37.0 37.0 35.0 37.0 7 35.408630611113374 37.0 36.0 37.0 35.0 37.0 8 35.407642882933516 37.0 36.0 37.0 35.0 37.0 9 37.10369907073783 39.0 38.0 39.0 35.0 39.0 10-11 37.111887938627305 39.0 38.0 39.0 35.0 39.0 12-13 37.07975685993688 39.0 38.0 39.0 35.0 39.0 14-15 38.546418390054434 41.0 39.0 41.0 35.0 41.0 16-17 38.53823435656413 41.0 39.0 41.0 35.0 41.0 18-19 38.50460884423113 41.0 39.0 41.0 35.0 41.0 20-21 38.527002385473835 41.0 39.0 41.0 34.5 41.0 22-23 38.479397150423836 41.0 39.0 41.0 34.0 41.0 24-25 38.459697578117094 41.0 39.0 41.0 34.0 41.0 26-27 38.41254360401436 40.5 39.0 41.0 34.0 41.0 28-29 38.379732839016015 40.0 39.0 41.0 34.0 41.0 30-31 38.37616762070513 40.0 39.0 41.0 34.0 41.0 32-33 38.4486467459206 41.0 39.0 41.0 34.0 41.0 34-35 38.412339954949445 41.0 39.0 41.0 34.0 41.0 36-37 38.33656014370251 41.0 39.0 41.0 34.0 41.0 38-39 38.24959338170754 41.0 39.0 41.0 34.0 41.0 40-41 38.17839839377088 41.0 38.0 41.0 33.0 41.0 42-43 38.05039815809392 40.5 38.0 41.0 33.0 41.0 44-45 37.90335235367256 40.0 38.0 41.0 33.0 41.0 46-47 37.76045174438718 40.0 38.0 41.0 33.0 41.0 48-49 37.627904756293404 40.0 37.5 41.0 33.0 41.0 50-51 37.4829114564685 40.0 37.0 41.0 32.0 41.0 52-53 37.316837607999716 40.0 37.0 41.0 32.0 41.0 54-55 37.14264997590354 40.0 36.5 41.0 31.5 41.0 56-57 36.96079181415361 40.0 36.0 41.0 31.0 41.0 58-59 36.7454450139971 39.0 35.0 41.0 31.0 41.0 60-61 36.49878097603498 39.0 35.0 41.0 31.0 41.0 62-63 36.21737543490708 39.0 35.0 41.0 31.0 41.0 64-65 35.927651101412096 38.0 35.0 40.5 30.0 41.0 66-67 35.613837410091506 37.5 35.0 40.0 30.0 41.0 68-69 35.288049818483415 37.0 35.0 40.0 30.0 41.0 70-71 34.930821107591065 36.5 34.5 39.0 29.5 41.0 72-73 34.566235800840886 36.0 34.0 39.0 29.0 41.0 74-75 34.19515260838496 35.5 34.0 38.5 29.0 40.0 76-77 33.87626400653852 35.0 34.0 37.0 29.0 39.5 78-79 33.52971750490616 35.0 34.0 37.0 29.0 39.0 80-81 33.19649083050446 35.0 34.0 36.5 28.0 39.0 82-83 33.01286871735836 35.0 34.0 36.0 28.0 38.0 84-85 32.775552141261144 35.0 34.0 36.0 28.0 37.0 86-87 32.556878821252624 35.0 34.0 35.0 28.0 37.0 88-89 32.23536770893291 35.0 34.0 35.0 27.0 36.0 90-91 32.06854588826797 35.0 34.0 35.0 27.0 36.0 92-93 31.91207209902058 35.0 33.0 35.0 26.5 36.0 94-95 31.75985333641526 35.0 33.0 35.0 26.0 36.0 96-97 31.593981021961767 35.0 33.0 35.0 25.5 35.0 98-99 31.412918723174748 35.0 33.0 35.0 25.0 35.0 100 31.25656277240706 35.0 33.0 35.0 25.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.18626181973770528 1101 2 0.2091146529610981 1101 3 0.1931228656849875 1101 4 0.09727480802816757 1101 5 0.14467582756896746 1101 6 0.1276113755699484 1101 7 0.13929038005181837 1101 8 0.08582867721737131 1101 9 0.1592081434046264 1101 10-11 0.20329802905797578 1101 12-13 0.2282218272210983 1101 14-15 0.23838541844067862 1101 16-17 0.1381910617321367 1101 18-19 0.16847010757579284 1101 20-21 0.12606925233937005 1101 22-23 0.15500733081429274 1101 24-25 0.1427646131725666 1101 26-27 0.2216211924314777 1101 28-29 0.21753203083480344 1101 30-31 0.27117300084911733 1101 32-33 0.232343292183522 1101 34-35 0.2329611744519866 1101 36-37 0.24787507771920048 1101 38-39 0.23857944101387574 1101 40-41 0.27068692068777267 1101 42-43 0.20715244573631253 1101 44-45 0.12081440485896877 1101 46-47 0.2208413351320928 1101 48-49 0.1905202538413917 1101 50-51 0.15737584662528548 1101 52-53 0.17189867395197211 1101 54-55 0.18326655663420865 1101 56-57 0.1770535843145069 1101 58-59 0.17579741936248183 1101 60-61 0.17097993842759962 1101 62-63 0.22573589177813602 1101 64-65 0.21416114449193202 1101 66-67 0.1699064906417007 1101 68-69 0.20058260565917863 1101 70-71 0.16470939352653602 1101 72-73 0.18049913772065906 1101 74-75 0.14642138489461587 1101 76-77 0.08321301909923307 1101 78-79 0.08045679908835268 1101 80-81 0.15777136834645944 1101 82-83 0.032519742200484814 1101 84-85 0.09597688258551784 1101 86-87 0.12995620620755943 1101 88-89 0.15944804469418017 1101 90-91 0.19926241443884862 1101 92-93 0.21953264878373702 1101 94-95 0.17935368189283807 1101 96-97 0.17654954968580583 1101 98-99 0.26271830224724724 1101 100 0.28299111130859345 1102 1 0.24656293282216168 1102 2 0.16392415081288547 1102 3 0.23241757223278015 1102 4 0.21393318664100747 1102 5 0.06275079518459847 1102 6 0.11747167438534944 1102 7 0.1133738819010972 1102 8 0.16452120448764873 1102 9 0.21825514616536168 1102 10-11 0.226190430893773 1102 12-13 0.2087653922565309 1102 14-15 0.26796169608462606 1102 16-17 0.24148391606988184 1102 18-19 0.2739513832561613 1102 20-21 0.23345663972675368 1102 22-23 0.23216908853817841 1102 24-25 0.26177557989726097 1102 26-27 0.3268920429827489 1102 28-29 0.31458753266063866 1102 30-31 0.32234843913711586 1102 32-33 0.3372489056694903 1102 34-35 0.3684612653040631 1102 36-37 0.3199848504717835 1102 38-39 0.2477331803094458 1102 40-41 0.35499077691876124 1102 42-43 0.38966284683356633 1102 44-45 0.26911882378667684 1102 46-47 0.28224050398214473 1102 48-49 0.2539482726213933 1102 50-51 0.27139754561770246 1102 52-53 0.2108241576660106 1102 54-55 0.2722843577795331 1102 56-57 0.25767545797737057 1102 58-59 0.2650311319299661 1102 60-61 0.24164055610448543 1102 62-63 0.22269397673361624 1102 64-65 0.308895616113837 1102 66-67 0.25969373266575957 1102 68-69 0.20198407218751413 1102 70-71 0.24103702369410485 1102 72-73 0.29680491649878604 1102 74-75 0.2611429848289717 1102 76-77 0.2102330358629061 1102 78-79 0.235234152889781 1102 80-81 0.29873468169233774 1102 82-83 0.1694462223566333 1102 84-85 0.20349835591311205 1102 86-87 0.19533265617737072 1102 88-89 0.372930680224961 1102 90-91 0.37162017232687106 1102 92-93 0.37855390816934786 1102 94-95 0.365028032886638 1102 96-97 0.38056296062439543 1102 98-99 0.3714598896381567 1102 100 0.4387513790584485 1103 1 0.07593503207904817 1103 2 0.08228293331347203 1103 3 0.11068332564181915 1103 4 0.12217096060403776 1103 5 0.10326234112598343 1103 6 0.12690744313574243 1103 7 0.1179994671825142 1103 8 0.10810547075433874 1103 9 0.09617232563645217 1103 10-11 0.1691633696481034 1103 12-13 0.1577396655380383 1103 14-15 0.11177878601692726 1103 16-17 0.10678554772897542 1103 18-19 0.17939639104089622 1103 20-21 0.12620992899407213 1103 22-23 0.14014223830749728 1103 24-25 0.14447522573428273 1103 26-27 0.2483348379617354 1103 28-29 0.265897525075502 1103 30-31 0.24750705966943798 1103 32-33 0.21394162793384908 1103 34-35 0.2509736210867288 1103 36-37 0.23824353202736148 1103 38-39 0.27160349164209663 1103 40-41 0.19708614428444093 1103 42-43 0.18118490360230055 1103 44-45 0.1675725516229818 1103 46-47 0.21371668495314822 1103 48-49 0.20350351007027712 1103 50-51 0.16145816965173765 1103 52-53 0.03047170561488599 1103 54-55 0.12137191759087074 1103 56-57 0.0353743453010793 1103 58-59 0.09507450976654042 1103 60-61 0.1206240113892676 1103 62-63 0.20789286120576378 1103 64-65 0.16319812034839742 1103 66-67 0.2029913379308752 1103 68-69 0.1202562587019429 1103 70-71 0.13388548642144826 1103 72-73 0.18444844945989303 1103 74-75 0.17830254067094842 1103 76-77 0.16039702301950598 1103 78-79 0.1451056618750286 1103 80-81 0.1801130543676095 1103 82-83 0.19836962391813984 1103 84-85 0.18520173728561673 1103 86-87 0.2141339770170987 1103 88-89 0.25143128760591793 1103 90-91 0.26390523645831365 1103 92-93 0.27185811763308365 1103 94-95 0.3017004064630022 1103 96-97 0.2868039625463439 1103 98-99 0.32834425913108944 1103 100 0.31093670724377986 1104 1 0.1568688653106669 1104 2 0.14395061711321233 1104 3 0.13528714720194657 1104 4 0.11130719135110922 1104 5 0.12144250764394826 1104 6 0.08761006173536146 1104 7 0.08173947571067686 1104 8 0.12769536620123034 1104 9 0.08590211263390302 1104 10-11 0.10288113404506305 1104 12-13 0.12874677613003627 1104 14-15 0.05304412290978178 1104 16-17 0.1428880878765426 1104 18-19 0.10414494611362102 1104 20-21 0.12299179729095755 1104 22-23 0.09624153442805294 1104 24-25 0.08882234450419446 1104 26-27 0.14793174243322937 1104 28-29 0.14976046077546812 1104 30-31 0.18309442994119962 1104 32-33 0.1610834912924517 1104 34-35 0.18150556271351093 1104 36-37 0.23090870378567985 1104 38-39 0.18672308722881326 1104 40-41 0.12650664548442592 1104 42-43 0.08898582541380051 1104 44-45 0.09377319184768851 1104 46-47 0.07355282875504798 1104 48-49 0.06337307917068102 1104 50-51 -0.0070621381324400545 1104 52-53 -0.06939732938120358 1104 54-55 -0.04890101846808648 1104 56-57 -0.02724222734280346 1104 58-59 -0.028862666432722506 1104 60-61 -0.005208271976705703 1104 62-63 0.05601314237227939 1104 64-65 -0.016881999676222392 1104 66-67 -0.029306715915595305 1104 68-69 0.007787593551199734 1104 70-71 -2.625165099914284E-4 1104 72-73 0.03443472473025366 1104 74-75 -0.034976562103857134 1104 76-77 -0.08235124136037797 1104 78-79 -0.05775956697115703 1104 80-81 -0.01252112182902465 1104 82-83 0.01723460409937161 1104 84-85 0.040203974394657394 1104 86-87 0.038188437269667475 1104 88-89 0.1262034176927216 1104 90-91 0.09181306231228348 1104 92-93 0.052363485381413 1104 94-95 0.07501861267804699 1104 96-97 0.06808724009058409 1104 98-99 0.02187415452544883 1104 100 0.030172036230144528 1105 1 0.08583114101000433 1105 2 0.059342148668321215 1105 3 0.043556776543912434 1105 4 0.060008136463665096 1105 5 0.042821510864669676 1105 6 0.03276440760687649 1105 7 0.035168938402151184 1105 8 0.0406563671172151 1105 9 -0.009835805416940957 1105 10-11 0.02254983811381095 1105 12-13 0.023051829253041944 1105 14-15 0.02283893344405641 1105 16-17 0.08149513379829187 1105 18-19 0.014040135283870825 1105 20-21 0.005282544980936166 1105 22-23 0.04601781417124329 1105 24-25 0.019125886460187758 1105 26-27 0.03991527577810672 1105 28-29 0.06165404169255595 1105 30-31 0.056170330204174945 1105 32-33 0.0934723804158537 1105 34-35 0.04258372550587808 1105 36-37 0.06935946211292787 1105 38-39 0.03866892582097847 1105 40-41 0.04755397287159013 1105 42-43 0.022624382122671705 1105 44-45 0.014841624919448293 1105 46-47 -0.011737775416285956 1105 48-49 0.006314388482927313 1105 50-51 -0.07144977779930173 1105 52-53 -0.06021540682218074 1105 54-55 -0.10677808207639572 1105 56-57 -0.07393676054837783 1105 58-59 -0.05099229332642352 1105 60-61 -0.13899203579565977 1105 62-63 -0.01834102367880064 1105 64-65 -0.06663136967153349 1105 66-67 -0.0626343858902274 1105 68-69 -0.0886128319394004 1105 70-71 -0.11794359273106636 1105 72-73 -0.017133111050640082 1105 74-75 -0.04038911492083486 1105 76-77 -0.07809865696929563 1105 78-79 6.131636401036644E-4 1105 80-81 0.019410934751093123 1105 82-83 0.0312936282569396 1105 84-85 -0.04424704564307547 1105 86-87 0.007638064332226691 1105 88-89 0.06707172666305183 1105 90-91 0.08379965598142292 1105 92-93 0.0937935221475179 1105 94-95 0.03899817393307714 1105 96-97 0.11276312009210088 1105 98-99 0.056755097358593076 1105 100 0.1846759893248695 1106 1 0.19334383500853392 1106 2 0.13753544581776111 1106 3 0.148873357387739 1106 4 0.19625101933695532 1106 5 0.14258188429987229 1106 6 0.15765198311262907 1106 7 0.14774467600078367 1106 8 0.11833714647006133 1106 9 0.12603036014271396 1106 10-11 0.11171418532597244 1106 12-13 0.17404291472549716 1106 14-15 0.17609808455394926 1106 16-17 0.16661260242567977 1106 18-19 0.1914960051670107 1106 20-21 0.1273574013682932 1106 22-23 0.13576735743728108 1106 24-25 0.11117224410011062 1106 26-27 0.16862012302473772 1106 28-29 0.1936947302268095 1106 30-31 0.224029363819227 1106 32-33 0.23732575540864076 1106 34-35 0.23319113429315053 1106 36-37 0.18696189410281505 1106 38-39 0.1948627664949214 1106 40-41 0.14169253694276307 1106 42-43 0.20956048049576737 1106 44-45 0.12450124068634238 1106 46-47 0.1229625744473779 1106 48-49 0.14913309120930052 1106 50-51 0.17763100856935665 1106 52-53 0.11808246234404862 1106 54-55 0.1651502081721361 1106 56-57 0.10521637344694312 1106 58-59 0.09136102662846923 1106 60-61 0.08423272972876106 1106 62-63 0.08362257953380237 1106 64-65 0.041625827895181544 1106 66-67 0.06614973206587393 1106 68-69 -0.0029580280578045404 1106 70-71 0.051326104689493945 1106 72-73 0.032378825723114346 1106 74-75 0.09248925857001211 1106 76-77 0.018811176429160525 1106 78-79 0.023497802984586258 1106 80-81 0.09034265589721713 1106 82-83 0.08519176337922119 1106 84-85 0.06427233798078902 1106 86-87 0.08936175067020002 1106 88-89 0.1480865328671399 1106 90-91 0.12861613380557912 1106 92-93 0.12550337844818316 1106 94-95 0.18115970796242564 1106 96-97 0.18291925032670164 1106 98-99 0.19804162952310733 1106 100 0.23475189324683754 1107 1 0.10988874448288044 1107 2 0.08387744845014566 1107 3 0.09169421680187995 1107 4 0.02751634999303576 1107 5 0.07897695530698456 1107 6 0.02676557205661112 1107 7 0.023344815098447214 1107 8 -0.009513981641880775 1107 9 0.09019518526105941 1107 10-11 0.014913595095713106 1107 12-13 0.018838016767837473 1107 14-15 0.004711866065918002 1107 16-17 -0.013223354880310012 1107 18-19 0.05855292583647298 1107 20-21 0.06582947246514692 1107 22-23 0.0532059319854028 1107 24-25 0.02677838458874504 1107 26-27 0.1594532787468097 1107 28-29 0.10075963872896665 1107 30-31 0.09707166708204085 1107 32-33 0.11288710046805761 1107 34-35 0.0584769248315169 1107 36-37 0.06296060688858063 1107 38-39 0.026101806010622397 1107 40-41 -0.008536675061513677 1107 42-43 0.014440723767101815 1107 44-45 0.07587092730528866 1107 46-47 0.03678418917676396 1107 48-49 -0.0017509454646145173 1107 50-51 0.02318925477969458 1107 52-53 0.03698013675830225 1107 54-55 0.03138111963573209 1107 56-57 0.012477705980089127 1107 58-59 0.07291843432669509 1107 60-61 0.07628450901966488 1107 62-63 0.15315131142680372 1107 64-65 0.08293622039737159 1107 66-67 0.1116795604747054 1107 68-69 0.14434819338418237 1107 70-71 0.1665595887119551 1107 72-73 0.18930959098459255 1107 74-75 0.20314714937087075 1107 76-77 0.12496440166266609 1107 78-79 0.12597973337592805 1107 80-81 0.11897826489818186 1107 82-83 0.15224024751386622 1107 84-85 0.12287926276335526 1107 86-87 0.15663832505379816 1107 88-89 0.26710161767184815 1107 90-91 0.25920399435644015 1107 92-93 0.2583393067754933 1107 94-95 0.2298341904593073 1107 96-97 0.32048185829565057 1107 98-99 0.3017181124836412 1107 100 0.35664650059507963 1108 1 -0.046494829192305076 1108 2 -0.055317553080730164 1108 3 -0.003852334002104385 1108 4 -0.05900531738158321 1108 5 -0.15776952579059866 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-0.15311025215677176 2214 40-41 -0.12177511274300201 2214 42-43 -0.14201350463846296 2214 44-45 -0.11552828018459138 2214 46-47 -0.07618234179630434 2214 48-49 -0.10218084309908448 2214 50-51 -0.02054937540584234 2214 52-53 -0.016145137043949376 2214 54-55 -0.10718272852448507 2214 56-57 -0.042518128715229864 2214 58-59 -0.007571427407370379 2214 60-61 -0.028728013572539624 2214 62-63 5.744414816177823E-5 2214 64-65 -0.05911255136780369 2214 66-67 -0.02366375877817717 2214 68-69 -0.05638928598938264 2214 70-71 0.002487900539875909 2214 72-73 -0.04508634786792243 2214 74-75 0.0021654067865597426 2214 76-77 -0.03502280615521869 2214 78-79 -0.10435785919732155 2214 80-81 -0.03503269954852328 2214 82-83 -0.14312959475356735 2214 84-85 -0.13886897802200338 2214 86-87 -0.13471238082247794 2214 88-89 -0.18663540059063166 2214 90-91 -0.3165213394295776 2214 92-93 -0.2468944585756816 2214 94-95 -0.28197937673766305 2214 96-97 -0.28342696035570114 2214 98-99 -0.28137524018715254 2214 100 -0.23066510940715546 2215 1 -0.19362729369186127 2215 2 -0.19798343297467014 2215 3 -0.20954987462789276 2215 4 -0.27866046493101493 2215 5 -0.21310967519506363 2215 6 -0.15751159639415135 2215 7 -0.12373579162449033 2215 8 -0.16195015591672046 2215 9 -0.2125448387462754 2215 10-11 -0.2768383124343359 2215 12-13 -0.2728600580367484 2215 14-15 -0.3794753571584337 2215 16-17 -0.29505070228887575 2215 18-19 -0.26734936550376887 2215 20-21 -0.24846552318982873 2215 22-23 -0.27779126679272537 2215 24-25 -0.22622609868292187 2215 26-27 -0.3273908260691343 2215 28-29 -0.28265637069841176 2215 30-31 -0.28225566112504197 2215 32-33 -0.29794697897999356 2215 34-35 -0.2929689604555392 2215 36-37 -0.3985817740732003 2215 38-39 -0.3427408959999383 2215 40-41 -0.27084208175987357 2215 42-43 -0.2523607098703877 2215 44-45 -0.21666069753506179 2215 46-47 -0.219373177389393 2215 48-49 -0.12332567543692363 2215 50-51 -0.1605977958507765 2215 52-53 -0.29531501360575163 2215 54-55 -0.19221423711683627 2215 56-57 -0.22962095287870454 2215 58-59 -0.20896809479152267 2215 60-61 -0.26189932432025387 2215 62-63 -0.18807739687306935 2215 64-65 -0.23845811165440267 2215 66-67 -0.22071159729783574 2215 68-69 -0.18156301292970767 2215 70-71 -0.1782569164428196 2215 72-73 -0.1610252828561869 2215 74-75 -0.10102434509690994 2215 76-77 -0.2566439939931868 2215 78-79 -0.20931942022276218 2215 80-81 -0.24495619550726389 2215 82-83 -0.26532628287714743 2215 84-85 -0.257377485734537 2215 86-87 -0.2879435928560241 2215 88-89 -0.15393784356749762 2215 90-91 -0.19421355393254203 2215 92-93 -0.18648249250208337 2215 94-95 -0.22825919292954566 2215 96-97 -0.29515652788015956 2215 98-99 -0.36102493712860095 2215 100 -0.31998244338926085 2216 1 -0.11530373140881522 2216 2 -0.07131233339013932 2216 3 -0.12255340956347638 2216 4 -0.09782831057773933 2216 5 -0.08881980481722707 2216 6 -0.02083677532829853 2216 7 0.00763403187871603 2216 8 -0.027029301070292888 2216 9 -0.12092087202924517 2216 10-11 -0.129256314263003 2216 12-13 -0.1624171109631405 2216 14-15 -0.22951033153610467 2216 16-17 -0.19192899735045899 2216 18-19 -0.21262357358645545 2216 20-21 -0.26001275734859064 2216 22-23 -0.2123563994371196 2216 24-25 -0.1758346061590217 2216 26-27 -0.26944561674524437 2216 28-29 -0.29084584055712526 2216 30-31 -0.29666932617804775 2216 32-33 -0.25261397715249956 2216 34-35 -0.23338711298298165 2216 36-37 -0.278369761659782 2216 38-39 -0.213567642649366 2216 40-41 -0.27631528886313816 2216 42-43 -0.2988635727932234 2216 44-45 -0.2526687414060902 2216 46-47 -0.20095535290888478 2216 48-49 -0.10226151337278111 2216 50-51 -0.10811662553216195 2216 52-53 -0.1577169857078502 2216 54-55 -0.13093248867151175 2216 56-57 -0.17742007283563055 2216 58-59 -0.21016021284502528 2216 60-61 -0.1576892393888798 2216 62-63 -0.14478619827072237 2216 64-65 -0.08368876684972548 2216 66-67 -0.08968706617971378 2216 68-69 -0.15354052980549682 2216 70-71 -0.1907499386653484 2216 72-73 -0.08052782009045245 2216 74-75 -0.07968985679941198 2216 76-77 -0.12536222550187404 2216 78-79 -0.15103723170874161 2216 80-81 -0.12787522121496409 2216 82-83 -0.11487010072548998 2216 84-85 -0.16782645250463446 2216 86-87 -0.12044851532870382 2216 88-89 -0.11738478427637489 2216 90-91 -0.1598103694010753 2216 92-93 -0.12183345790575473 2216 94-95 -0.07192278139502761 2216 96-97 -0.10253764860260972 2216 98-99 -0.129465313361397 2216 100 -0.10587881923717646 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 32538.0 3 5876.0 4 3908.0 5 3128.0 6 2756.0 7 3075.0 8 3553.0 9 3956.0 10 4262.0 11 4723.0 12 5055.0 13 5572.0 14 6082.0 15 6555.0 16 6804.0 17 6695.0 18 6744.0 19 6503.0 20 6470.0 21 6957.0 22 8076.0 23 10461.0 24 15544.0 25 21241.0 26 22450.0 27 20461.0 28 22289.0 29 28543.0 30 34805.0 31 43142.0 32 57224.0 33 79047.0 34 113457.0 35 181069.0 36 351610.0 37 726723.0 38 1049442.0 39 392157.0 40 662.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.923116003181626 18.830328735498387 13.024167733674776 24.22238752764521 2 15.772553090829453 19.51495593863473 39.72834168823453 24.98414928230128 3 18.778039836484236 24.725243993250636 29.33110128248192 27.165614887783214 4 12.610870374758793 16.173200804747236 37.49525445778704 33.72067436270693 5 13.771241835992276 37.74355244778919 34.67838638370315 13.806819332515383 6 32.3905149466675 36.71667372246915 17.45783818068475 13.4349731501786 7 28.75709871451096 31.802905781769635 21.658263335792995 17.78173216792641 8 26.092590432479117 36.208801403621585 19.591684158549285 18.10692400535001 9 25.823298779776525 16.65766110437205 19.917855975069703 37.60118414078172 10-11 24.98898072499501 26.416462260437484 27.948659018073425 20.64589799649408 12-13 25.34840250766098 23.52661469847373 28.09240891778663 23.03257387607866 14-15 22.604663363301153 25.237957304658032 24.999731796094316 27.1576475359465 16-17 22.853043241490457 28.67446293027454 25.572578717391035 22.899915110843963 18-19 22.95723060418099 27.859369451726717 26.993328850643316 22.190071093448978 20-21 23.85857815659834 27.04693586157072 26.652117877829163 22.44236810400178 22-23 23.398334583153968 26.864021982740844 26.591247306892136 23.146396127213055 24-25 23.24728360446289 26.986385722499634 26.71246818184909 23.053862491188383 26-27 23.105018691278108 27.109742129868874 26.60107342635153 23.18416575250149 28-29 23.096576709001226 27.12684169840298 26.699466733231837 23.07711485936396 30-31 23.254872196495292 27.44721941380877 26.363902991397264 22.934005398298673 32-33 23.06448676015815 27.099655999087297 26.737370829659486 23.098486411095067 34-35 23.113581296004963 27.011858620058177 26.82782366731799 23.04673641661887 36-37 23.32386262767889 27.08397703158723 26.416137417647544 23.176022923086336 38-39 23.24452761974236 27.267739813351167 26.494836077141095 22.99289648976538 40-41 23.08674380927473 27.146271951791988 26.728497997552676 23.038486241380607 42-43 23.056754176034037 27.084028331762035 26.628381318442234 23.230836173761695 44-45 23.109359107547878 27.07706700461782 26.718839991128178 23.094733896706124 46-47 23.090517365113854 27.060145979666792 26.73363318944873 23.115703465770622 48-49 23.19519237684069 27.058940128007485 26.629962163400204 23.11590533175162 50-51 23.076432634085037 27.159802756442158 26.662403068098822 23.10136154137398 52-53 23.218124372616245 27.00930507182921 26.568527378860924 23.204043176693627 54-55 23.189842146197726 27.133241154754256 26.626082710533677 23.050833988514338 56-57 22.971701462592176 27.074014929589808 26.678173822002183 23.276109785815834 58-59 23.033793589609168 26.827667226765833 26.835780153876694 23.30275902974831 60-61 23.10420930272612 27.059591727217047 26.68083394999189 23.15536502006495 62-63 22.991894607232155 27.09128953550736 26.63418466396235 23.28263119329813 64-65 23.02318002907645 27.00403664967897 26.70129170070325 23.271491620541333 66-67 22.977538376749028 26.982018131737306 26.633844539871816 23.40659895164185 68-69 23.071474899037 27.02102384208875 26.713606221112556 23.193895037761695 70-71 23.03455025416663 26.906446104392405 26.757558411036698 23.301445230404273 72-73 23.03928256053131 27.00441676694473 26.72341158421204 23.23288908831192 74-75 23.01093216245791 27.102081810906718 26.622091576595164 23.26489445004021 76-77 23.130794985388224 27.00760253434934 26.57814039843317 23.283462081829263 78-79 23.183849314089578 27.02504134679899 26.66583371266985 23.125275626441585 80-81 23.16327939667001 26.813499565946387 26.889281429001795 23.133939608381812 82-83 23.01993510876745 26.945392378029805 26.839365494519175 23.195307018683575 84-85 23.016509828408314 26.936345780127503 26.606297269976388 23.440847121487796 86-87 23.060363679874328 27.024293317649338 26.666206379966468 23.249136622509862 88-89 22.961063277019043 26.891969419615545 26.67409823807928 23.47286906528613 90-91 22.985785628226107 26.892066620187748 26.757295996991793 23.364851754594355 92-93 23.16129746101486 26.9462021613002 26.711763920919918 23.18073645676503 94-95 23.01315858259082 26.88128462584935 26.71235728709624 23.393199504463595 96-97 23.081460949484956 26.755337538171403 26.83144100423751 23.33176050810613 98-99 22.81446465378329 26.997621520950126 26.758071663154364 23.429842162112223 100 23.099572588657537 26.650341780150157 26.63648274778415 23.613602883408156 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 446.0 1 418.5 2 392.0 3 360.0 4 290.0 5 240.0 6 218.0 7 208.0 8 228.5 9 221.0 10 201.5 11 208.0 12 209.0 13 247.5 14 275.0 15 328.0 16 422.5 17 529.0 18 702.0 19 989.5 20 1400.5 21 2067.0 22 3096.5 23 4638.5 24 6962.0 25 9958.5 26 13660.0 27 18360.0 28 24333.5 29 31171.5 30 38270.5 31 45416.5 32 52221.5 33 60674.5 34 69501.5 35 76226.5 36 82491.0 37 90038.0 38 96226.5 39 99871.0 40 103821.0 41 108079.5 42 112125.5 43 114104.5 44 113398.5 45 114330.5 46 114375.5 47 110954.0 48 111313.0 49 112151.5 50 110049.5 51 109309.5 52 107209.0 53 106317.5 54 106582.0 55 103334.0 56 96139.5 57 89569.5 58 81597.0 59 71412.0 60 64591.5 61 57898.5 62 50085.5 63 42007.0 64 34513.0 65 28251.5 66 22920.5 67 18319.5 68 15063.5 69 12716.0 70 10016.5 71 7059.5 72 5019.0 73 3769.5 74 2682.0 75 1697.5 76 1051.5 77 719.5 78 501.5 79 339.0 80 221.5 81 142.5 82 86.0 83 55.0 84 39.5 85 34.5 86 29.0 87 24.0 88 18.5 89 13.5 90 12.5 91 9.0 92 4.5 93 3.5 94 4.0 95 5.0 96 4.5 97 2.5 98 2.0 99 2.0 100 5.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.32242420946242994 2 0.11394074537370662 3 0.06175944936193485 4 0.03767809850994753 5 0.04060895300510784 6 0.008520628532321734 7 0.006435793891434501 8 0.013929112600710353 9 0.016588032142711463 10-11 0.019352704166496707 12-13 0.01436723002524463 14-15 0.01708657955683667 16-17 0.017947706908507485 18-19 0.017826846929325617 20-21 0.02261592360440716 22-23 0.014306800035653695 24-25 0.02334108347949837 26-27 0.01767577195534828 28-29 0.017434051996984543 30-31 0.018416039327837228 32-33 0.02288785855756636 34-35 0.013929112600710353 36-37 0.008686811003696804 38-39 0.012267287886959662 40-41 0.008747240993287738 42-43 0.007704823672844122 44-45 0.007886113641616926 46-47 0.007553748698866786 48-49 0.0036560143702515247 50-51 0.006163858938275298 52-53 0.007160953766525714 54-55 0.002930854495160313 56-57 0.0024171995836373716 58-59 0.0024776295732283063 60-61 0.0033840794170923207 62-63 0.002719349531592043 64-65 0.0019186521695121637 66-67 0.0017826846929325617 68-69 0.0033387569248991195 70-71 0.0022208021174668353 72-73 0.002039512148694032 74-75 0.0020697271434894997 76-77 0.003822196841626594 78-79 0.0032783269353081853 80-81 0.0035049393962741887 82-83 0.0023265545992509702 84-85 0.0030668219717399155 86-87 0.0025229520654215065 88-89 0.5921383605041675 90-91 0.6028344686617628 92-93 0.5993446367628863 94-95 0.5874701438082677 96-97 0.5848867617532553 98-99 0.5824544546722201 100 0.5844184293339255 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3309615.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.89019170480829 #Duplication Level Percentage of deduplicated Percentage of total 1 72.06026321709075 38.83341399067965 2 14.856393135922152 16.012277482736856 3 5.556896780775154 8.983866983994151 4 2.534733068108895 5.46389003843621 5 1.4456773143758364 3.8953913807503104 6 0.8637952450481956 2.7930054809609475 7 0.5535796275703367 2.0882758577549274 8 0.3884645328189983 1.674754251530769 9 0.2873897601841765 1.3938740343291736 >10 1.351245431559645 12.579166090225174 >50 0.06627826550067707 2.447271647388477 >100 0.033710198652937035 3.2252791666141825 >500 0.0014048414225657588 0.49663905805459796 >1k 1.6858096967265653E-4 0.11289453654463842 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 3.0214994795467145E-5 0.0 0.0 0.0 6 0.0 3.0214994795467145E-5 0.0 0.0 0.0 7 0.0 3.0214994795467145E-5 0.0 0.0 0.0 8 0.0 3.0214994795467145E-5 0.0 0.0 0.0 9 0.0 3.0214994795467145E-5 0.0 0.0 0.0 10-11 0.0 3.0214994795467145E-5 0.0 0.0 0.0 12-13 0.0 3.0214994795467145E-5 0.0 0.0 0.0 14-15 0.0 3.0214994795467145E-5 0.0 0.0 0.0 16-17 0.0 3.0214994795467145E-5 0.0 0.0 0.0 18-19 0.0 3.0214994795467145E-5 0.0 1.5107497397733572E-5 0.0 20-21 0.0 3.0214994795467145E-5 0.0 4.532249219320072E-5 0.0 22-23 0.0 3.0214994795467145E-5 0.0 1.0575248178413501E-4 0.0 24-25 0.0 3.0214994795467145E-5 0.0 3.1725744535240506E-4 0.0 26-27 0.0 3.0214994795467145E-5 0.0 4.3811742453427364E-4 0.0 28-29 0.0 3.0214994795467145E-5 0.0 0.0014201047553869558 0.0 30-31 0.0 3.0214994795467145E-5 0.0 0.00486461416207021 0.0 32-33 0.0 3.0214994795467145E-5 0.0 0.012539222840118866 0.0 34-35 0.0 3.0214994795467145E-5 0.0 0.0230842560237369 0.0 36-37 0.0 3.0214994795467145E-5 0.0 0.0441894298883707 0.0 38-39 0.0 4.532249219320072E-5 0.0 0.07931436133810126 0.0 40-41 0.0 6.042998959093429E-5 0.0 0.12002906682499323 0.0 42-43 0.0 6.042998959093429E-5 0.0 0.15986753746281668 0.0 44-45 0.0 7.553748698866786E-5 0.0 0.2073957242760865 0.0 46-47 0.0 9.064498438640143E-5 0.0 0.2642754519785534 0.0 48-49 0.0 1.2085997918186858E-4 0.0 0.33000817315609215 0.0 50-51 0.0 1.2085997918186858E-4 0.0 0.39900411377154144 0.0 52-53 0.0 1.2085997918186858E-4 0.0 0.46990359905910506 0.0 54-55 0.0 1.2085997918186858E-4 0.0 0.5517106974678323 0.0 56-57 0.0 1.3596747657960214E-4 0.0 0.643367884179882 0.0 58-59 0.0 1.5107497397733572E-4 0.0 0.7496793433677331 0.0 60-61 0.0 1.5107497397733572E-4 0.0 0.8647682585436675 0.0 62-63 1.5107497397733572E-5 1.5107497397733572E-4 0.0 0.980416151123318 0.0 64-65 3.0214994795467145E-5 1.5107497397733572E-4 0.0 1.1004905404405045 0.0 66-67 3.0214994795467145E-5 1.5107497397733572E-4 0.0 1.2383162392000278 0.0 68-69 3.0214994795467145E-5 1.5107497397733572E-4 0.0 1.3941198598628541 0.0 70-71 3.0214994795467145E-5 1.5107497397733572E-4 0.0 1.5645626455040844 0.0 72-73 3.0214994795467145E-5 1.5107497397733572E-4 0.0 1.743586489667227 0.0 74-75 3.0214994795467145E-5 1.5107497397733572E-4 0.0 1.9235621061664272 0.0 76-77 3.0214994795467145E-5 1.5107497397733572E-4 0.0 2.120458119751089 0.0 78-79 3.0214994795467145E-5 1.5107497397733572E-4 0.0 2.344472091164682 0.0 80-81 3.0214994795467145E-5 1.5107497397733572E-4 0.0 2.591026448695694 0.0 82-83 3.0214994795467145E-5 1.5107497397733572E-4 0.0 2.8441374600973224 0.0 84-85 3.0214994795467145E-5 1.5107497397733572E-4 0.0 3.0965384191212575 0.0 86-87 3.0214994795467145E-5 1.5107497397733572E-4 0.0 3.3627627382641183 0.0 88 3.0214994795467145E-5 1.8128996877280287E-4 0.0 3.57972150839297 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 4370 0.0 34.384792 1 GGTATCA 3450 0.0 30.993021 1 TCAACGC 4840 0.0 30.476942 4 CAACGCA 4945 0.0 29.638912 5 ATCAACG 5005 0.0 29.38279 3 AACGCAG 5195 0.0 28.293186 6 GTACATG 11250 0.0 27.299345 1 TATCAAC 5615 0.0 26.45494 2 TACATGG 11635 0.0 25.37247 2 ACATGGG 11300 0.0 24.947416 3 ACGCAGA 6315 0.0 22.977785 7 CGCAGAG 6515 0.0 22.056168 8 CATGGGG 8435 0.0 21.107714 4 GAGTACA 7565 0.0 19.862728 1 ATATCCG 1020 0.0 18.061241 94 GCAGAGT 7760 0.0 17.851856 9 AGTACAT 7920 0.0 17.568556 2 CTAGACT 935 0.0 16.580189 4 ATGGGGG 4255 0.0 16.449802 5 TAGACAG 1465 0.0 16.032688 5 >>END_MODULE