FastQCFastQC Report
Wed 25 May 2016
SRR1033129_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033129_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3309615
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52800.15953517252006655No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43020.12998490761009968No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48850.042.0965921
GTATCAA75800.039.2974661
TCAACGC91500.031.9056174
ATCAACG93450.031.3907683
CAACGCA94350.030.942795
TATCAAC96500.030.5934872
AACGCAG96350.030.3875166
GTACATG107300.026.5329631
ACGCAGA112400.025.672077
TACATGG112350.025.2313752
CGCAGAG116750.024.7554328
ACATGGG109250.024.742473
GCAGAGT129900.022.1405289
GAGTACT72950.019.7119112-13
GAGTACA73450.019.4765431
AGAGTAC116500.019.28180710-11
CATGGGG84350.018.8377174
GTGGTAT24250.018.6290021
AGTACTT75550.018.34932712-13
TATACTG17050.018.1982365
Produced by FastQC (version 0.11.5)