##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033129_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3309615
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.75437414925905	34.0	31.0	34.0	31.0	34.0
2	32.85667728723734	34.0	31.0	34.0	31.0	34.0
3	32.95488115687172	34.0	31.0	34.0	31.0	34.0
4	36.3731947673672	37.0	37.0	37.0	35.0	37.0
5	36.310617095946206	37.0	37.0	37.0	35.0	37.0
6	36.392502451191454	37.0	37.0	37.0	35.0	37.0
7	36.36470949037879	37.0	37.0	37.0	35.0	37.0
8	36.36483881055651	37.0	37.0	37.0	35.0	37.0
9	38.18652290372143	39.0	39.0	39.0	37.0	39.0
10-11	38.16765998462057	39.0	39.0	39.0	37.0	39.0
12-13	38.12733777191607	39.0	39.0	39.0	37.0	39.0
14-15	39.66913734679109	41.0	40.0	41.0	37.0	41.0
16-17	39.67584900358501	41.0	40.0	41.0	37.0	41.0
18-19	39.67771976498777	41.0	40.0	41.0	37.0	41.0
20-21	39.662022168741686	41.0	40.0	41.0	37.0	41.0
22-23	39.60022132483688	41.0	40.0	41.0	37.0	41.0
24-25	39.549367977846366	41.0	40.0	41.0	37.0	41.0
26-27	39.44761052267408	41.0	39.0	41.0	36.5	41.0
28-29	39.36388718929543	41.0	39.0	41.0	36.0	41.0
30-31	39.28322931821375	41.0	39.0	41.0	36.0	41.0
32-33	39.337093438360654	41.0	39.0	41.0	36.0	41.0
34-35	39.28904646008675	41.0	39.0	41.0	35.0	41.0
36-37	39.21747151859053	41.0	39.0	41.0	35.0	41.0
38-39	39.136257993754555	41.0	39.0	41.0	35.0	41.0
40-41	39.02814088647773	41.0	39.0	41.0	35.0	41.0
42-43	38.918718944650664	41.0	39.0	41.0	35.0	41.0
44-45	38.77472425040375	41.0	39.0	41.0	35.0	41.0
46-47	38.57644287930771	41.0	38.0	41.0	35.0	41.0
48-49	38.400660197636284	41.0	38.0	41.0	35.0	41.0
50-51	38.25861633452834	40.0	38.0	41.0	34.5	41.0
52-53	38.105173562483856	40.0	37.5	41.0	34.0	41.0
54-55	37.94301149831627	40.0	37.0	41.0	34.0	41.0
56-57	37.76072352826537	40.0	37.0	41.0	34.0	41.0
58-59	37.54753649593684	40.0	36.0	41.0	33.5	41.0
60-61	37.330986232537626	39.5	36.0	41.0	33.0	41.0
62-63	37.07353257100901	39.0	35.0	41.0	33.0	41.0
64-65	36.802100848588125	39.0	35.0	41.0	33.0	41.0
66-67	36.49862340483712	38.0	35.0	41.0	32.5	41.0
68-69	36.16247675333838	37.0	35.0	40.0	32.0	41.0
70-71	35.82006396514398	37.0	35.0	39.5	32.0	41.0
72-73	35.46777631235053	36.0	35.0	39.0	32.0	41.0
74-75	35.13740631463176	36.0	35.0	39.0	31.0	41.0
76-77	34.81153548071301	35.0	35.0	37.5	31.0	40.0
78-79	34.495271504389486	35.0	35.0	37.0	31.0	39.0
80-81	34.184735988929226	35.0	34.5	37.0	31.0	39.0
82-83	33.89844558959275	35.0	34.0	36.0	31.0	38.0
84-85	33.657960215916354	35.0	34.0	36.0	31.0	37.0
86-87	33.45660462017486	35.0	34.0	35.5	30.0	37.0
88-89	33.272538950905165	35.0	34.0	35.0	30.0	36.5
90-91	33.097992213595845	35.0	34.0	35.0	30.0	36.0
92-93	32.95596360906026	35.0	34.0	35.0	30.0	36.0
94-95	32.821691646913614	35.0	34.0	35.0	30.0	36.0
96-97	32.71115507392854	35.0	34.0	35.0	30.0	35.5
98-99	32.625116365498705	35.0	34.0	35.0	30.0	35.0
100	32.53665879566052	35.0	34.0	35.0	30.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.08682798116437596
1101	2	0.12543305884189238
1101	3	0.12218767206409353
1101	4	0.08724302113058258
1101	5	0.07721783497090229
1101	6	0.10125424947928252
1101	7	0.11306126033934305
1101	8	0.1380089663609354
1101	9	0.19383923115292845
1101	10-11	0.12903011621631322
1101	12-13	0.17322256926855317
1101	14-15	0.20285526594110337
1101	16-17	0.179941523504084
1101	18-19	0.24651908980197135
1101	20-21	0.25189874949736435
1101	22-23	0.2542449572573915
1101	24-25	0.2556113685986574
1101	26-27	0.29057127762422397
1101	28-29	0.274187715515815
1101	30-31	0.25605472792326367
1101	32-33	0.23485460141889547
1101	34-35	0.2678607680742786
1101	36-37	0.3268764826059325
1101	38-39	0.2957524871380244
1101	40-41	0.30560278088026394
1101	42-43	0.2962534155079197
1101	44-45	0.22282700030482516
1101	46-47	0.25997062316024255
1101	48-49	0.2110593291806282
1101	50-51	0.22163137881444328
1101	52-53	0.26916141928744963
1101	54-55	0.21609959127924583
1101	56-57	0.2232072518219752
1101	58-59	0.23998581523395757
1101	60-61	0.22268058413312275
1101	62-63	0.22521109367986014
1101	64-65	0.2359839688680907
1101	66-67	0.23847245474144074
1101	68-69	0.3058934899623651
1101	70-71	0.26912783796517914
1101	72-73	0.33583351934912287
1101	74-75	0.2879623788103203
1101	76-77	0.25836305743438004
1101	78-79	0.2119902265155318
1101	80-81	0.22588048956001927
1101	82-83	0.23380671766607009
1101	84-85	0.25992404565542415
1101	86-87	0.17752571182690247
1101	88-89	0.2398125570563252
1101	90-91	0.2534349488207681
1101	92-93	0.22429465440698237
1101	94-95	0.17015486303611738
1101	96-97	0.25348908480533083
1101	98-99	0.2790833911249777
1101	100	0.2678939441687973
1102	1	0.13290388660788466
1102	2	0.1174547226657694
1102	3	0.08191982842344459
1102	4	0.08171166544178021
1102	5	0.09093408342494769
1102	6	0.09712677522558977
1102	7	0.1468372718746238
1102	8	0.15223492426418517
1102	9	0.18487575368718012
1102	10-11	0.15837836101282932
1102	12-13	0.16049673738592674
1102	14-15	0.23304038144046757
1102	16-17	0.12985778719783525
1102	18-19	0.15589865804945902
1102	20-21	0.1770903301476281
1102	22-23	0.17593647050127714
1102	24-25	0.22968026377881046
1102	26-27	0.1977121569550846
1102	28-29	0.1844374473556556
1102	30-31	0.21103394917444973
1102	32-33	0.20769508275904514
1102	34-35	0.23554652539959164
1102	36-37	0.19276050846654158
1102	38-39	0.20812461723819098
1102	40-41	0.18475976740484157
1102	42-43	0.1603106933452949
1102	44-45	0.19718240190030656
1102	46-47	0.180631604245427
1102	48-49	0.11986349313639266
1102	50-51	0.13277275050493387
1102	52-53	0.13975697515562047
1102	54-55	0.12983581546699696
1102	56-57	0.12917026623522077
1102	58-59	0.1235234660923652
1102	60-61	0.12076638366069403
1102	62-63	0.1282821751712362
1102	64-65	0.18114439018726358
1102	66-67	0.15772373985870303
1102	68-69	0.11816911757436088
1102	70-71	0.19544178756864738
1102	72-73	0.12342905763301104
1102	74-75	0.16676474917027662
1102	76-77	0.18083435110931845
1102	78-79	0.14149886332410233
1102	80-81	0.16115402464357942
1102	82-83	0.07500819240040357
1102	84-85	0.12629956365878314
1102	86-87	0.1743910456057023
1102	88-89	0.14427760245305876
1102	90-91	0.11189109657555463
1102	92-93	0.16444843473252746
1102	94-95	0.1207287785261002
1102	96-97	0.08187774412176907
1102	98-99	0.1800820605816824
1102	100	0.18649484565583663
1103	1	0.12063463149077336
1103	2	0.13364493271122058
1103	3	0.10882494582191526
1103	4	0.06758000349918802
1103	5	0.021329297377029377
1103	6	0.09348490757190575
1103	7	0.05776627388782174
1103	8	0.09878681398889455
1103	9	0.11494406551028646
1103	10-11	0.12206904726777879
1103	12-13	0.09359919371437542
1103	14-15	0.10899261221101852
1103	16-17	0.16559895736145336
1103	18-19	0.15800892193225735
1103	20-21	0.12935052872984443
1103	22-23	0.14212630414162675
1103	24-25	0.1178153589921962
1103	26-27	0.1781515471788211
1103	28-29	0.17001928409849398
1103	30-31	0.1949462810040643
1103	32-33	0.1894496780380095
1103	34-35	0.2018728914364445
1103	36-37	0.21172776692566941
1103	38-39	0.19195272557441712
1103	40-41	0.2401268821456668
1103	42-43	0.1996976092757876
1103	44-45	0.18368971613583795
1103	46-47	0.061470348877378456
1103	48-49	0.09072148005346037
1103	50-51	0.03472775298578057
1103	52-53	-0.04456406417871506
1103	54-55	0.06415139122422886
1103	56-57	0.1228109134238693
1103	58-59	0.12987050019125235
1103	60-61	0.08376932076112098
1103	62-63	0.06931744889187996
1103	64-65	0.08061619117162167
1103	66-67	0.14812429191951537
1103	68-69	0.10585387259737189
1103	70-71	0.13948713868047236
1103	72-73	0.1580618174238282
1103	74-75	0.11572898498236839
1103	76-77	0.11038135327080312
1103	78-79	0.13029898651122807
1103	80-81	0.10368672911424426
1103	82-83	0.03634602738668491
1103	84-85	0.11422282264260275
1103	86-87	0.14349619380095646
1103	88-89	0.13510853382756238
1103	90-91	0.07333425655342296
1103	92-93	0.10317262674475813
1103	94-95	0.113840359826753
1103	96-97	0.11131150537827494
1103	98-99	0.10673256844341239
1103	100	0.10100722425093522
1104	1	0.03337691565352685
1104	2	0.07138653829882458
1104	3	0.07021813502880292
1104	4	0.04361903339064099
1104	5	-0.034408296299169194
1104	6	-9.941174513201645E-4
1104	7	0.01237568165369396
1104	8	0.050963420395170544
1104	9	0.07341360273954933
1104	10-11	0.04922494618721629
1104	12-13	0.06875298948865094
1104	14-15	0.06208299666980821
1104	16-17	0.06610495552799023
1104	18-19	0.05619640392811931
1104	20-21	0.07245760613731278
1104	22-23	0.09319070398787233
1104	24-25	0.06875371897674398
1104	26-27	-0.02464726886076818
1104	28-29	0.02402688326458957
1104	30-31	0.02467194401576478
1104	32-33	0.059727604907756415
1104	34-35	0.07611128933364597
1104	36-37	0.019254172254804303
1104	38-39	0.047674690132659236
1104	40-41	0.042089474705505836
1104	42-43	0.05718800634294041
1104	44-45	0.053618198692952035
1104	46-47	0.0227732438103061
1104	48-49	-0.0015099508470086676
1104	50-51	-0.02236642186245774
1104	52-53	-0.017983969108151143
1104	54-55	0.01456991886499992
1104	56-57	0.04098287137146883
1104	58-59	0.019699534276732322
1104	60-61	-0.025190103947245746
1104	62-63	-0.022535140069315673
1104	64-65	0.06068302425701688
1104	66-67	0.06540300418526357
1104	68-69	0.044815074734835036
1104	70-71	0.05826123357066848
1104	72-73	0.10445440247993787
1104	74-75	0.10577271148943623
1104	76-77	0.05380339899537745
1104	78-79	0.004081009785913636
1104	80-81	0.03812531543643871
1104	82-83	0.03536088949583416
1104	84-85	0.07231638545984254
1104	86-87	0.06895210874353097
1104	88-89	0.04267302692317543
1104	90-91	0.0756885620908534
1104	92-93	0.06285641948075948
1104	94-95	0.006373165651815782
1104	96-97	0.08760567952605669
1104	98-99	0.09966487991553663
1104	100	0.12863188726176134
1105	1	0.005367772670958004
1105	2	0.04388182117831718
1105	3	0.08029567731404086
1105	4	0.07036922228652287
1105	5	0.00991722933790129
1105	6	0.021713066501604317
1105	7	-0.07093710881328974
1105	8	-0.018542453316854335
1105	9	-0.0064608606484171105
1105	10-11	-0.0344689074153095
1105	12-13	-0.00879341135895828
1105	14-15	-0.01883820314615292
1105	16-17	0.0017009589555527782
1105	18-19	0.0022911135772147873
1105	20-21	0.021777580979332356
1105	22-23	0.04968555631140248
1105	24-25	0.004701132052979062
1105	26-27	0.0404343257159141
1105	28-29	0.03532887184218936
1105	30-31	0.060600361975858164
1105	32-33	0.029734949685995105
1105	34-35	0.03862337233808688
1105	36-37	-0.03794054457022611
1105	38-39	-0.08142793836929485
1105	40-41	0.028822124777477143
1105	42-43	0.016854093306797324
1105	44-45	0.039956700104916365
1105	46-47	0.10262923972424431
1105	48-49	0.035542028233038536
1105	50-51	0.04129085558864887
1105	52-53	0.028503833916722954
1105	54-55	0.019632961544921557
1105	56-57	-0.08315812726510075
1105	58-59	-0.07280393760838422
1105	60-61	-0.04690764234904066
1105	62-63	-0.06660781960482609
1105	64-65	-0.12083113976792248
1105	66-67	-0.1590234488355904
1105	68-69	-0.16440832049443088
1105	70-71	-0.11503996987546827
1105	72-73	-0.1512275644010188
1105	74-75	-0.18154593791558682
1105	76-77	-0.13019036638538495
1105	78-79	-0.12679565703552242
1105	80-81	-0.13144271451675138
1105	82-83	-0.07844043425270542
1105	84-85	-0.06175916444877316
1105	86-87	-0.051544782389129296
1105	88-89	-0.030397385861803627
1105	90-91	-0.04669085058015554
1105	92-93	0.023164224785041654
1105	94-95	-0.03860948547544041
1105	96-97	-0.03675281319036827
1105	98-99	-0.06012414766114915
1105	100	-0.043007560106993026
1106	1	0.06825545220439722
1106	2	0.06421887088056621
1106	3	0.02637107158098928
1106	4	0.03541418603609969
1106	5	0.005313318783969123
1106	6	0.01915297740874422
1106	7	0.05740350040993292
1106	8	0.06391702729468562
1106	9	0.08929537425899525
1106	10-11	0.062343631872273875
1106	12-13	0.06880771423673337
1106	14-15	0.09483022836230504
1106	16-17	0.10887084544037862
1106	18-19	0.1237205346228265
1106	20-21	0.10889738095586665
1106	22-23	0.1598436380731343
1106	24-25	0.12943446030589456
1106	26-27	0.07586170007728299
1106	28-29	0.09016188522991087
1106	30-31	0.10744084358372419
1106	32-33	0.07798272831185216
1106	34-35	0.12723004244815428
1106	36-37	0.10968782579575276
1106	38-39	0.12708535681247923
1106	40-41	0.1575217303190044
1106	42-43	0.13612284318836032
1106	44-45	0.21295742305701282
1106	46-47	0.2516303723357538
1106	48-49	0.18542908964847982
1106	50-51	0.1672112889244417
1106	52-53	0.1714097908726444
1106	54-55	0.187315349698018
1106	56-57	0.16206036918001132
1106	58-59	0.10193886603452285
1106	60-61	0.18661054173077218
1106	62-63	0.18300131047776347
1106	64-65	0.07384171533643524
1106	66-67	0.12479937911269445
1106	68-69	0.17678880027727217
1106	70-71	0.16422688896017945
1106	72-73	0.2098323527235877
1106	74-75	0.15020147721817523
1106	76-77	0.15208982775124014
1106	78-79	0.153743081382828
1106	80-81	0.16819609168088334
1106	82-83	0.2155987591859727
1106	84-85	0.2621357732333962
1106	86-87	0.23954008525177528
1106	88-89	0.20862962867666823
1106	90-91	0.20582952251635334
1106	92-93	0.20167258873194527
1106	94-95	0.13874945318411136
1106	96-97	0.14721585512219093
1106	98-99	0.19273066638130132
1106	100	0.21965472610703785
1107	1	-0.031964037920417354
1107	2	-0.04193844408589342
1107	3	-0.008945758861955255
1107	4	-0.02031905867856665
1107	5	-0.059872817815914914
1107	6	-0.10758060196292263
1107	7	-0.09374040958041974
1107	8	-0.04388622990885693
1107	9	-0.05083383325825963
1107	10-11	-0.06825626417839459
1107	12-13	-0.03382308243389076
1107	14-15	-0.0265621597799921
1107	16-17	0.013366350005924232
1107	18-19	0.004085913701338484
1107	20-21	-0.02061534722786007
1107	22-23	-0.0509593097129013
1107	24-25	-0.016987444023328635
1107	26-27	-0.0030303001246352324
1107	28-29	-0.0814292873035285
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2216	12-13	-0.08569167542702161
2216	14-15	-0.22567693540323575
2216	16-17	-0.21269143048893113
2216	18-19	-0.15873585141776658
2216	20-21	-0.19607527547150738
2216	22-23	-0.18497212718055778
2216	24-25	-0.20293866336056254
2216	26-27	-0.2804442326213774
2216	28-29	-0.291050929835329
2216	30-31	-0.2658817363657775
2216	32-33	-0.22245958965584833
2216	34-35	-0.1766313104288031
2216	36-37	-0.3214699565371646
2216	38-39	-0.24136816246276993
2216	40-41	-0.2619624086407555
2216	42-43	-0.2801269088395202
2216	44-45	-0.19622011721224908
2216	46-47	-0.2189007615099854
2216	48-49	-0.24952616553207463
2216	50-51	-0.18066791151565553
2216	52-53	-0.1388394644742803
2216	54-55	-0.13538548829705377
2216	56-57	-0.1627559506203724
2216	58-59	-0.17659848891434393
2216	60-61	-0.17274864606995521
2216	62-63	-0.16961578158917234
2216	64-65	-0.20404642482511548
2216	66-67	-0.19584280471899973
2216	68-69	-0.20776780413951457
2216	70-71	-0.18308169096226123
2216	72-73	-0.12809517900645773
2216	74-75	-0.16097091755423776
2216	76-77	-0.1357594579751904
2216	78-79	-0.15590045894510496
2216	80-81	-0.16311301224799735
2216	82-83	-0.16318714386184752
2216	84-85	-0.1679025681790307
2216	86-87	-0.14200991162053356
2216	88-89	-0.12307981376680743
2216	90-91	-0.10418338074056521
2216	92-93	-0.08549771215785285
2216	94-95	-0.10822106756872785
2216	96-97	-0.11736621156084226
2216	98-99	-0.098670531144883
2216	100	-0.1241107826152188
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	22.0
3	6.0
4	15.0
5	27.0
6	77.0
7	174.0
8	370.0
9	625.0
10	1065.0
11	1671.0
12	2483.0
13	3882.0
14	4426.0
15	5885.0
16	7664.0
17	11623.0
18	13815.0
19	7921.0
20	5703.0
21	5500.0
22	5801.0
23	6281.0
24	7093.0
25	8852.0
26	11299.0
27	14631.0
28	18987.0
29	24594.0
30	31905.0
31	40734.0
32	52543.0
33	68737.0
34	97352.0
35	157670.0
36	314957.0
37	716088.0
38	1169458.0
39	488205.0
40	1474.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.66253577197147	18.886784413143097	13.228681447324211	24.22199836756122
2	16.100114244138002	19.721813559972432	39.167066264319416	25.011005931570146
3	18.881440892671804	24.729915715272018	29.60093545623887	26.787707935817306
4	12.89414764881663	16.318085816757865	37.2117560679355	33.57601046649001
5	13.600245681233435	37.421018077882884	34.847229073568954	14.131507167314725
6	32.28875815164761	36.830475642576296	17.202314353335606	13.678451852440487
7	28.440725410958034	31.920414858971036	21.67458033964433	17.9642793904266
8	26.423727302805016	35.501154971665805	19.509186141548614	18.565931583980564
9	26.00692347227514	15.936042030317157	20.056012625048684	38.00102187235902
10-11	25.113325645595484	25.93685429309498	27.963957572509905	20.985862488799626
12-13	25.74497335792834	23.107551784724205	28.323007358861986	22.824467498485472
14-15	22.75756535170367	24.717160911484626	25.62820439800865	26.89706933880306
16-17	22.602281534257003	28.28516307788066	26.14758816357794	22.964967224284397
18-19	22.5688252466904	27.15226005207858	28.204059446207324	22.0748552550237
20-21	23.43994694246914	26.30961607316863	28.088025344337638	22.162411640024597
22-23	22.910156896200863	26.161402474038848	28.06436169111845	22.864078938641835
24-25	22.810317342334994	26.281978068728296	28.12498866936668	22.78271591957003
26-27	22.713699815368567	26.40779389083132	28.018110528977136	22.86039576482298
28-29	22.70489527056326	26.486136044772714	28.109739201537952	22.699229483126075
30-31	22.86530162561141	26.811034480674095	27.716187124021165	22.60747676969334
32-33	22.720504234189036	26.41736531289478	28.07923162011376	22.782898832802424
34-35	22.808741881178843	26.335838871812538	28.135308144964593	22.720111102044026
36-37	22.97135952530879	26.49148329367348	27.73430481465648	22.80285236636125
38-39	22.95186111057802	26.635654327464977	27.7380208870885	22.674463674868505
40-41	22.865271428815383	26.51328208222694	27.839895980534436	22.781550508423244
42-43	22.864694446031095	26.473702868571912	27.716170438208458	22.94543224718853
44-45	22.894218661720004	26.558098749840013	27.674316800177944	22.87336578826204
46-47	22.956285972793793	26.6080968288035	27.542067354910614	22.893549843492096
48-49	23.06268449157947	26.690213698606957	27.285497211086394	22.961604598727178
50-51	23.055421130457077	26.803124047682324	27.152224727405795	22.989230094454804
52-53	23.1284762416719	26.717886982920618	27.08505001920271	23.06858675620477
54-55	23.07925205603008	26.860066067930767	27.124383115436068	22.936298760603087
56-57	22.837371392295253	26.778790291012488	27.212522376656633	23.171315940035623
58-59	22.87262947962199	26.530883257399836	27.427340199351306	23.16914706362687
60-61	22.97351745855347	26.76021888475495	27.227692931054566	23.038570725637015
62-63	22.83378203210689	26.727732001438255	27.231664536480572	23.206821429974287
64-65	22.83083740692776	26.631049280168824	27.310814297878306	23.227299015025114
66-67	22.785545426088262	26.6145475157693	27.23678358325306	23.363123474889385
68-69	22.93179887834676	26.63944568176194	27.273136553017768	23.155618886873526
70-71	22.870846706038975	26.552044626514004	27.296212656385872	23.28089601106115
72-73	22.83231505713763	26.650469324583824	27.218514401982237	23.29870121629631
74-75	22.83375158648398	26.758653655420737	27.07376740713413	23.33382735096115
76-77	22.925992751695333	26.622752014102	27.072111249647428	23.37914398455524
78-79	22.910677801097044	26.72094087105022	27.113539524548784	23.25484180330395
80-81	22.977141030421215	26.508717977228653	27.271484331329667	23.24265666102046
82-83	22.82125072837363	26.597855722422004	27.23781230931416	23.343081239890203
84-85	22.808023918262148	26.58901893126219	26.973369933874284	23.62958721660138
86-87	22.77456818559163	26.667428085570794	27.06832118160128	23.4896825472363
88-89	22.666684795663546	26.519232599562187	27.072952594183914	23.741130010590357
90-91	22.69530525312015	26.522685122796798	27.111953236842723	23.670056387240322
92-93	22.77816990138427	26.565303385832912	27.118132205145372	23.53839450763745
94-95	22.636137435925328	26.48927443222248	27.09664115010356	23.77794698174863
96-97	22.678952083550506	26.355134962828004	27.185941567221565	23.77997138639993
98-99	22.382965994534107	26.553858983597788	27.16642268058369	23.896752341284408
100	22.619972413709753	26.19395307309158	27.035501108134934	24.15057340506373
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	60.0
1	53.5
2	142.5
3	258.0
4	193.0
5	133.0
6	346.5
7	807.5
8	1359.5
9	1626.5
10	1320.0
11	994.5
12	998.5
13	1157.5
14	1323.5
15	1524.5
16	1733.0
17	1924.0
18	2183.0
19	2512.0
20	2996.5
21	3676.5
22	4610.5
23	6045.5
24	8115.0
25	10886.0
26	14407.0
27	18598.5
28	24195.5
29	30868.5
30	37677.0
31	44687.5
32	51738.5
33	59916.5
34	67974.0
35	74882.5
36	81322.5
37	88983.0
38	95405.5
39	98404.0
40	102485.5
41	107021.5
42	111084.0
43	113343.0
44	112288.5
45	113319.5
46	113727.0
47	110634.0
48	110849.0
49	111381.5
50	109399.0
51	108904.0
52	107174.5
53	106311.0
54	106815.5
55	103786.5
56	96652.0
57	89991.0
58	81783.0
59	71732.5
60	64881.5
61	58094.0
62	50539.0
63	42286.0
64	34218.0
65	27784.5
66	22399.0
67	17782.0
68	14736.0
69	12205.5
70	9174.5
71	6408.5
72	4628.0
73	3474.5
74	2485.0
75	1533.0
76	865.5
77	570.0
78	392.5
79	226.0
80	127.0
81	61.5
82	24.5
83	13.0
84	4.0
85	2.0
86	1.0
87	1.0
88	1.5
89	1.0
90	0.5
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08780477487562753
2	0.0010877398126368173
3	0.0
4	1.5107497397733572E-4
5	0.03816153842667501
6	2.4171995836373719E-4
7	0.004622894203706473
8	3.0214994795467145E-4
9	1.2085997918186859E-4
10-11	4.6833241932974076E-4
12-13	0.0
14-15	1.0575248178413502E-4
16-17	0.0
18-19	3.0214994795467148E-5
20-21	0.0
22-23	1.9639746617053646E-4
24-25	9.064498438640143E-5
26-27	0.0014654272475801565
28-29	0.008399768553139867
30-31	0.035276006423707894
32-33	0.025229520654215066
34-35	0.028553170081716456
36-37	0.02578849805793121
38-39	0.02491226320886266
40-41	0.030320747277251283
42-43	0.022842536065373162
44-45	0.021709473760543142
46-47	0.015621152309256515
48-49	0.010620570670606701
50-51	0.007689716175446388
52-53	0.005619989031956889
54-55	0.004305636758354069
56-57	0.0025229520654215065
58-59	9.215573412617481E-4
60-61	5.891923985116093E-4
62-63	0.0013596747657960216
64-65	0.002689134536796576
66-67	5.740849011138758E-4
68-69	0.0010877398126368173
70-71	3.927949323410729E-4
72-73	5.589774037161422E-4
74-75	4.985474141252079E-4
76-77	4.0790242973880647E-4
78-79	3.0214994795467148E-5
80-81	5.136549115229414E-4
82-83	4.6833241932974076E-4
84-85	3.0214994795467145E-4
86-87	1.3596747657960217E-4
88-89	0.0
90-91	3.0214994795467148E-5
92-93	3.0214994795467148E-5
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3309615.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.731172604561046
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.21094367993635	36.320944462359016
2	15.800532335363645	16.347601309692894
3	5.849659677736125	9.078292634007253
4	2.780954672790389	5.754481847343208
5	1.509465370442984	3.9043206809496835
6	0.911564538771179	2.8293781497221357
7	0.6007288716832065	2.1753486264711013
8	0.44449093882170415	1.839522998187917
9	0.30620829323664517	1.425646266333564
>10	1.4705491973965703	13.051759344368202
>50	0.07495380526203649	2.635844177148915
>100	0.03772929914552244	3.508176057945414
>500	0.0016936911335593803	0.5547038949295958
>1k	4.672251377141524E-4	0.41445686252880204
>5k	5.840314221426905E-5	0.1595226880122521
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5280	0.15953517252006655	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4302	0.12998490761009968	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	3.0214994795467145E-5	0.0	0.0	0.0
6	0.0	3.0214994795467145E-5	0.0	0.0	0.0
7	0.0	3.0214994795467145E-5	0.0	0.0	0.0
8	0.0	3.0214994795467145E-5	0.0	0.0	0.0
9	0.0	9.064498438640143E-5	0.0	0.0	0.0
10-11	0.0	9.064498438640143E-5	0.0	0.0	0.0
12-13	0.0	9.064498438640143E-5	0.0	0.0	0.0
14-15	0.0	9.064498438640143E-5	0.0	0.0	0.0
16-17	0.0	9.064498438640143E-5	0.0	0.0	0.0
18-19	0.0	9.064498438640143E-5	0.0	1.5107497397733572E-5	0.0
20-21	0.0	9.064498438640143E-5	0.0	4.532249219320072E-5	0.0
22-23	0.0	9.064498438640143E-5	0.0	1.0575248178413501E-4	0.0
24-25	0.0	9.064498438640143E-5	0.0	3.1725744535240506E-4	0.0
26-27	0.0	9.064498438640143E-5	0.0	4.6833241932974076E-4	0.0
28-29	0.0	9.064498438640143E-5	0.0	0.001450319750182423	0.0
30-31	0.0	9.064498438640143E-5	0.0	0.004925044151661145	0.0
32-33	0.0	9.064498438640143E-5	0.0	0.012584545332312066	0.0
34-35	0.0	9.064498438640143E-5	0.0	0.023099363521134633	0.0
36-37	0.0	9.064498438640143E-5	0.0	0.04408367740658657	0.0
38-39	0.0	9.064498438640143E-5	0.0	0.07907264137973752	0.0
40-41	0.0	9.064498438640143E-5	0.0	0.1198779918510159	0.0
42-43	0.0	9.064498438640143E-5	0.0	0.15989775245761215	0.0
44-45	0.0	1.2085997918186858E-4	0.0	0.2075316917526661	0.0
46-47	0.0	1.5107497397733572E-4	0.0	0.2643963119577353	0.0
48-49	0.0	1.5107497397733572E-4	0.0	0.33005349564828534	0.0
50-51	0.0	1.5107497397733572E-4	0.0	0.3994573386934734	0.0
52-53	0.0	1.5107497397733572E-4	0.0	0.47070429642118494	0.0
54-55	0.0	1.5107497397733572E-4	0.0	0.552979727249242	0.0
56-57	0.0	1.8128996877280287E-4	0.0	0.64518078386761	0.0
58-59	0.0	2.1150496356827001E-4	0.0	0.7523986928993251	0.0
60-61	0.0	2.1150496356827001E-4	0.0	0.8678955105049984	0.0
62-63	0.0	2.1150496356827001E-4	0.0	0.9837095855560238	0.0
64-65	0.0	2.1150496356827001E-4	0.0	1.10425230729254	0.0
66-67	0.0	2.1150496356827001E-4	0.0	1.2427427359375636	0.0
68-69	0.0	2.1150496356827001E-4	0.0	1.3991959789884927	0.0
70-71	0.0	2.1150496356827001E-4	0.0	1.5698351620958935	0.0
72-73	0.0	2.1150496356827001E-4	0.0	1.7495992736315251	0.0
74-75	0.0	2.1150496356827001E-4	0.0	1.9295144601411343	0.0
76-77	0.0	2.1150496356827001E-4	0.0	2.1269392361347164	0.0
78-79	0.0	2.1150496356827001E-4	0.0	2.3526301397594587	0.0
80-81	0.0	2.1150496356827001E-4	0.0	2.6013750844131414	0.0
82-83	0.0	2.1150496356827001E-4	0.0	2.8570090478801915	0.0
84-85	0.0	2.1150496356827001E-4	0.0	3.1118876364773547	0.0
86-87	0.0	2.1150496356827001E-4	0.0	3.3808766276440005	0.0
88	0.0	2.1150496356827001E-4	0.0	3.6006907147810243	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4885	0.0	42.096592	1
GTATCAA	7580	0.0	39.297466	1
TCAACGC	9150	0.0	31.905617	4
ATCAACG	9345	0.0	31.390768	3
CAACGCA	9435	0.0	30.94279	5
TATCAAC	9650	0.0	30.593487	2
AACGCAG	9635	0.0	30.387516	6
GTACATG	10730	0.0	26.532963	1
ACGCAGA	11240	0.0	25.67207	7
TACATGG	11235	0.0	25.231375	2
CGCAGAG	11675	0.0	24.755432	8
ACATGGG	10925	0.0	24.74247	3
GCAGAGT	12990	0.0	22.140528	9
GAGTACT	7295	0.0	19.71191	12-13
GAGTACA	7345	0.0	19.476543	1
AGAGTAC	11650	0.0	19.281807	10-11
CATGGGG	8435	0.0	18.837717	4
GTGGTAT	2425	0.0	18.629002	1
AGTACTT	7555	0.0	18.349327	12-13
TATACTG	1705	0.0	18.198236	5
>>END_MODULE