FastQCFastQC Report
Wed 25 May 2016
SRR1033128_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033128_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3051892
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC34900.032.1703154
GTATCAA38950.030.9681
ATCAACG36500.030.8969153
CAACGCA37650.029.6953015
GTACATG79400.027.4158761
AACGCAG41200.027.3538766
GGTATCA26050.026.7691561
TACATGG77550.026.4341342
TATCAAC46650.024.8946292
ACATGGG82700.024.0911273
ACGCAGA48600.022.8024027
CGCAGAG49000.022.1374658
CATGGGG51650.018.9180264
GAGTACA57600.018.8142131
GCAGAGT58400.017.6093469
TGCACCG3501.5224941E-917.4482155
GTGTACG3151.4713441E-716.4536691
AGTACAT61700.016.307562
AGAGTAC49800.015.2813395
TACACTG20800.015.13168055
Produced by FastQC (version 0.11.5)