##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033128_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3051892 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.543597873057106 33.0 31.0 34.0 30.0 34.0 2 31.652198373992263 34.0 31.0 34.0 30.0 34.0 3 31.845844151758975 34.0 31.0 34.0 30.0 34.0 4 35.18962007829897 37.0 35.0 37.0 33.0 37.0 5 35.30143301270163 37.0 35.0 37.0 33.0 37.0 6 35.37961729969474 37.0 36.0 37.0 33.0 37.0 7 35.29810753460476 37.0 36.0 37.0 33.0 37.0 8 35.31181968431386 37.0 36.0 37.0 33.0 37.0 9 36.96477070617178 39.0 38.0 39.0 34.0 39.0 10-11 36.95721211628721 39.0 38.0 39.0 34.0 39.0 12-13 36.91030465691446 39.0 38.0 39.0 34.0 39.0 14-15 38.36057386696515 41.0 39.0 41.0 34.0 41.0 16-17 38.36960826267771 41.0 39.0 41.0 34.0 41.0 18-19 38.366130747745984 41.0 39.0 41.0 34.0 41.0 20-21 38.39518026850229 41.0 39.0 41.0 34.0 41.0 22-23 38.358001692065116 41.0 39.0 41.0 34.0 41.0 24-25 38.35179455891624 41.0 39.0 41.0 34.0 41.0 26-27 38.30649659293317 40.0 39.0 41.0 34.0 41.0 28-29 38.27561722367633 40.0 39.0 41.0 34.0 41.0 30-31 38.27354047915195 40.0 39.0 41.0 34.0 41.0 32-33 38.337355646923285 41.0 39.0 41.0 34.0 41.0 34-35 38.30696384406788 41.0 39.0 41.0 34.0 41.0 36-37 38.2283403213482 41.0 39.0 41.0 33.0 41.0 38-39 38.15450481209689 41.0 38.0 41.0 33.0 41.0 40-41 38.09808702273868 41.0 38.0 41.0 33.0 41.0 42-43 37.99188585310358 40.5 38.0 41.0 33.0 41.0 44-45 37.88116961543855 40.0 38.0 41.0 33.0 41.0 46-47 37.76524808217329 40.0 38.0 41.0 33.0 41.0 48-49 37.65319333056347 40.0 38.0 41.0 32.0 41.0 50-51 37.5463602578335 40.0 38.0 41.0 32.0 41.0 52-53 37.40701079854726 40.0 37.0 41.0 32.0 41.0 54-55 37.26335007922954 40.0 37.0 41.0 31.0 41.0 56-57 37.11624067955222 40.0 37.0 41.0 31.0 41.0 58-59 36.927081299076114 40.0 36.0 41.0 31.0 41.0 60-61 36.714995320935344 39.5 36.0 41.0 31.0 41.0 62-63 36.459303114264856 39.0 35.0 41.0 31.0 41.0 64-65 36.19125824242798 39.0 35.0 41.0 30.0 41.0 66-67 35.9027501628498 38.5 35.0 40.5 30.0 41.0 68-69 35.60052485474584 38.0 35.0 40.0 30.0 41.0 70-71 35.24974196334602 37.0 35.0 40.0 29.0 41.0 72-73 34.88589471711319 37.0 34.5 39.5 29.0 41.0 74-75 34.512601691016584 36.0 34.0 39.0 29.0 41.0 76-77 34.17036743108865 36.0 34.0 39.0 29.0 40.0 78-79 33.79167021637725 35.0 34.0 37.5 28.0 39.5 80-81 33.422392240616645 35.0 34.0 37.0 28.0 39.0 82-83 33.18456763869757 35.0 34.0 37.0 28.0 39.0 84-85 32.90185301445791 35.0 34.0 36.0 28.0 37.5 86-87 32.634096324509514 35.0 34.0 36.0 27.0 37.0 88-89 32.25983144226598 35.0 34.0 35.5 26.5 37.0 90-91 32.058907392528965 35.0 34.0 35.0 26.0 36.0 92-93 31.874492609830227 35.0 33.5 35.0 26.0 36.0 94-95 31.69746390108169 35.0 33.0 35.0 25.5 36.0 96-97 31.51681858335747 35.0 33.0 35.0 25.0 36.0 98-99 31.32496710237453 35.0 33.0 35.0 25.0 35.5 100 31.160965066915868 35.0 33.0 35.0 24.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.14625408317204247 1101 2 0.1230721378998787 1101 3 0.18882540300229422 1101 4 0.14330205844457566 1101 5 0.12494115478849466 1101 6 0.16192833138310903 1101 7 0.2120465879427087 1101 8 0.1675207432897352 1101 9 0.2824801045603351 1101 10-11 0.16446757366833253 1101 12-13 0.21147032923963138 1101 14-15 0.24482645534990866 1101 16-17 0.15676000419778546 1101 18-19 0.15228327847435708 1101 20-21 0.18550694162905046 1101 22-23 0.17271340964556003 1101 24-25 0.20308006974000392 1101 26-27 0.18174409969508076 1101 28-29 0.2307857578551662 1101 30-31 0.29011135306347313 1101 32-33 0.28584585878694213 1101 34-35 0.29848140126668454 1101 36-37 0.2618672435297924 1101 38-39 0.24514904304411544 1101 40-41 0.2211736797293966 1101 42-43 0.1922579691087023 1101 44-45 0.19538337205111844 1101 46-47 0.17521492724842602 1101 48-49 0.1828774428902591 1101 50-51 0.13768021929791985 1101 52-53 0.17159629410602406 1101 54-55 0.13084753836935903 1101 56-57 0.20324599587947745 1101 58-59 0.20326422824828683 1101 60-61 0.18398329062062402 1101 62-63 0.15801363123324563 1101 64-65 0.13992734228322234 1101 66-67 0.16436804110620784 1101 68-69 0.15568379844958002 1101 70-71 0.181750859824497 1101 72-73 0.1940389901724302 1101 74-75 0.12361827770077838 1101 76-77 0.15052046714581735 1101 78-79 0.1986355981554766 1101 80-81 0.1681922024688376 1101 82-83 0.13838888518036896 1101 84-85 0.13398293233812097 1101 86-87 0.20736461820360574 1101 88-89 0.34265358682209524 1101 90-91 0.3415842301770837 1101 92-93 0.32643166306003124 1101 94-95 0.32488324543196256 1101 96-97 0.28263414118586994 1101 98-99 0.3446940779368042 1101 100 0.34247653808900225 1102 1 0.18050503324632672 1102 2 0.1703062305006391 1102 3 0.2272180375884112 1102 4 0.1927742309988858 1102 5 0.11553955578659014 1102 6 0.09723455834944872 1102 7 0.1339897830694099 1102 8 0.1705183858699968 1102 9 0.21020744522930102 1102 10-11 0.22699352062142708 1102 12-13 0.17967040773388732 1102 14-15 0.2380669635710646 1102 16-17 0.18197474560952998 1102 18-19 0.19801215079655776 1102 20-21 0.17692695114167378 1102 22-23 0.2138775352208171 1102 24-25 0.27230108658840635 1102 26-27 0.32916723515405977 1102 28-29 0.31044538783055486 1102 30-31 0.2810091025203434 1102 32-33 0.3318424086251852 1102 34-35 0.30039483380718934 1102 36-37 0.3162073992827885 1102 38-39 0.25041144152969963 1102 40-41 0.33558432587494735 1102 42-43 0.30474035974054203 1102 44-45 0.2492921765839995 1102 46-47 0.2683280388999165 1102 48-49 0.26008250730350113 1102 50-51 0.26120421296653973 1102 52-53 0.1744140090115991 1102 54-55 0.21137589177408955 1102 56-57 0.15568890286269976 1102 58-59 0.188189723503001 1102 60-61 0.18213341596519683 1102 62-63 0.24109636901965814 1102 64-65 0.2631835234360409 1102 66-67 0.22825894359144883 1102 68-69 0.19540898862643274 1102 70-71 0.28309350259700494 1102 72-73 0.2510753880506229 1102 74-75 0.18660041582907638 1102 76-77 0.14481235346608656 1102 78-79 0.2465371835494068 1102 80-81 0.2990510568760243 1102 82-83 0.25403612909015294 1102 84-85 0.21444824085683933 1102 86-87 0.24753895256840508 1102 88-89 0.3197570072191098 1102 90-91 0.365388602138772 1102 92-93 0.33777831154528926 1102 94-95 0.3611544668535984 1102 96-97 0.34263429670558665 1102 98-99 0.3931314503620378 1102 100 0.34501345350225776 1103 1 0.12261103964492293 1103 2 0.09222504660833408 1103 3 0.1325188609238559 1103 4 0.12789920902078222 1103 5 0.07802829405468259 1103 6 0.09481878871032734 1103 7 0.0783839772726651 1103 8 -0.007078673990108086 1103 9 0.08968419505244185 1103 10-11 0.0885380222192893 1103 12-13 0.07007755949014438 1103 14-15 0.13429463668448705 1103 16-17 0.1594668296889452 1103 18-19 0.09041554137950669 1103 20-21 0.1440631710476623 1103 22-23 0.16023543042573607 1103 24-25 0.15037189298582376 1103 26-27 0.20372188514485856 1103 28-29 0.23646090226405647 1103 30-31 0.2500742045467419 1103 32-33 0.18225480523761917 1103 34-35 0.1942041678158546 1103 36-37 0.2163394540849879 1103 38-39 0.19252135456482478 1103 40-41 0.1751501690348718 1103 42-43 0.05625163814774936 1103 44-45 0.052099768757152276 1103 46-47 0.08608861011415314 1103 48-49 0.11345553136477093 1103 50-51 0.11951163358853734 1103 52-53 0.0860405158917601 1103 54-55 0.1214707611647512 1103 56-57 0.06934253459524342 1103 58-59 0.04800560398571463 1103 60-61 0.054489940543739124 1103 62-63 0.08453248290110338 1103 64-65 0.10478008345643275 1103 66-67 0.07400406319300146 1103 68-69 0.06786137000455739 1103 70-71 0.07335494467655934 1103 72-73 0.1779086218127901 1103 74-75 0.17719590941634777 1103 76-77 0.15710939648832323 1103 78-79 0.10331590321404605 1103 80-81 0.13210613630843682 1103 82-83 0.11846323028247241 1103 84-85 0.18048779709859275 1103 86-87 0.22099317384125072 1103 88-89 0.15488787403673854 1103 90-91 0.20458958117859538 1103 92-93 0.15009533871760183 1103 94-95 0.19156766367104439 1103 96-97 0.22686917250312533 1103 98-99 0.2857030622869381 1103 100 0.32883892952329674 1104 1 -0.04339458291397236 1104 2 -0.10171593027790138 1104 3 -0.08824268320547546 1104 4 -0.04047708714633558 1104 5 -0.11827721506128341 1104 6 -0.07589290620506972 1104 7 -0.04842503301271961 1104 8 -0.005431261898486639 1104 9 -0.011912629337174963 1104 10-11 -0.03662900678025238 1104 12-13 -0.04367850911808091 1104 14-15 -0.08876451895108772 1104 16-17 -0.09660710783861504 1104 18-19 -0.12110310123170365 1104 20-21 -0.10308521181318753 1104 22-23 -0.09025160372849683 1104 24-25 -0.06526817290050957 1104 26-27 0.0015560036694992618 1104 28-29 -0.009389162730514045 1104 30-31 0.024711930466359888 1104 32-33 -0.019286964992900835 1104 34-35 -0.06507199104322581 1104 36-37 -0.05922394506548301 1104 38-39 -0.07031200745365851 1104 40-41 -0.08527574723723319 1104 42-43 -0.03130642642879167 1104 44-45 -0.09339014753078345 1104 46-47 -0.17420371848695737 1104 48-49 -0.17116713701140185 1104 50-51 -0.13739537431815307 1104 52-53 -0.16359446544073108 1104 54-55 -0.13057202120048572 1104 56-57 -0.15441600223807228 1104 58-59 -0.17199423156745297 1104 60-61 -0.15170823808582412 1104 62-63 -0.18467052909483783 1104 64-65 -0.2298292946721503 1104 66-67 -0.20432397138265657 1104 68-69 -0.26161904348988685 1104 70-71 -0.2548797587103451 1104 72-73 -0.17455996110269467 1104 74-75 -0.20394684581007994 1104 76-77 -0.2639264591632866 1104 78-79 -0.2229644714222232 1104 80-81 -0.15808409771209142 1104 82-83 -0.1505553119603391 1104 84-85 -0.128734492735191 1104 86-87 -0.0683040479158592 1104 88-89 5.412525029413473E-4 1104 90-91 -0.01531242373786057 1104 92-93 -0.02891386609214308 1104 94-95 0.002124612090113942 1104 96-97 -0.07345083392045026 1104 98-99 -0.10110838830818736 1104 100 -0.020724679855188555 1105 1 0.07193838010923059 1105 2 0.06916665626525642 1105 3 0.07812665625904813 1105 4 0.1092570232502652 1105 5 0.03845138123585201 1105 6 0.05267814295697093 1105 7 0.031100307124098947 1105 8 0.041100391856844 1105 9 0.04713736602860763 1105 10-11 0.015774672387031785 1105 12-13 0.10193809789672059 1105 14-15 0.03825686461105704 1105 16-17 0.05053632630215077 1105 18-19 0.08153245373410556 1105 20-21 0.026442047543831393 1105 22-23 0.1245487030116692 1105 24-25 0.03167725635218943 1105 26-27 0.04586754587946018 1105 28-29 0.03117896389525754 1105 30-31 0.0729874877165102 1105 32-33 0.09725875302481768 1105 34-35 0.08072601800356694 1105 36-37 0.009839821875452515 1105 38-39 0.025979063796910395 1105 40-41 0.016907798112860917 1105 42-43 0.04370179862463175 1105 44-45 -0.0056590693542659665 1105 46-47 0.060683777515080806 1105 48-49 0.03133025380997623 1105 50-51 0.02159102809299185 1105 52-53 -0.013747616255813 1105 54-55 -0.05651054574439485 1105 56-57 -0.028271054352423164 1105 58-59 -0.04551727583402965 1105 60-61 -0.057737107395105625 1105 62-63 -0.026927086607074102 1105 64-65 0.03569388302827292 1105 66-67 -0.02997661341961333 1105 68-69 -0.07174908679527903 1105 70-71 -0.062276280590616295 1105 72-73 -0.08933081680537214 1105 74-75 -0.03584197942156209 1105 76-77 0.002094286843266957 1105 78-79 -0.0222705562963057 1105 80-81 -0.02459072917219629 1105 82-83 -0.004586962882420664 1105 84-85 -0.0027364058454111273 1105 86-87 0.12961734545672243 1105 88-89 0.15625349975408653 1105 90-91 0.14155886990672428 1105 92-93 0.1802369117389766 1105 94-95 0.21952641071725765 1105 96-97 0.3166078814062736 1105 98-99 0.3014737684823956 1105 100 0.3691492581742537 1106 1 0.10786964769438967 1106 2 0.0647477463709265 1106 3 0.08933042754674503 1106 4 0.1582467852430014 1106 5 0.0896484686490453 1106 6 0.12039150828305623 1106 7 0.07918047727422817 1106 8 0.10259762138991846 1106 9 0.040006480206074 1106 10-11 0.10461457532604612 1106 12-13 0.1329465622448467 1106 14-15 0.16329727829575091 1106 16-17 0.09617854328899256 1106 18-19 0.11943339155873645 1106 20-21 0.025063462935172254 1106 22-23 0.08852759306070368 1106 24-25 0.15977268905687225 1106 26-27 0.13050033218627988 1106 28-29 0.11646452112330508 1106 30-31 0.12170116840183454 1106 32-33 0.14230640615387813 1106 34-35 0.09147655502287932 1106 36-37 0.15522116710788225 1106 38-39 0.13239998434304567 1106 40-41 0.07733063409727947 1106 42-43 0.13123999286927557 1106 44-45 0.12214577759996104 1106 46-47 0.04763077255413606 1106 48-49 0.06073868643288449 1106 50-51 0.14724460350209512 1106 52-53 0.055049110292515024 1106 54-55 0.12609185041798554 1106 56-57 0.07532391326363097 1106 58-59 0.09788914632636647 1106 60-61 -0.003967480730473483 1106 62-63 0.09848571031520947 1106 64-65 0.05535279815461536 1106 66-67 0.13991073367090934 1106 68-69 0.12206529116334508 1106 70-71 0.16701856780980506 1106 72-73 0.14227979724790885 1106 74-75 0.202211517696675 1106 76-77 0.12122675457288778 1106 78-79 0.17648756524535258 1106 80-81 0.16405514548973343 1106 82-83 0.0777893532597318 1106 84-85 0.07470579690672707 1106 86-87 0.14959701462859698 1106 88-89 0.1298250558267 1106 90-91 0.07978626890848517 1106 92-93 0.11921719307879286 1106 94-95 0.1272110728626359 1106 96-97 0.06842618961939095 1106 98-99 0.1123329918127709 1106 100 0.1713175517362302 1107 1 -0.04083696576955731 1107 2 -0.05654345460166965 1107 3 -0.04507771165212304 1107 4 -0.04408198005594244 1107 5 -0.013381099944808739 1107 6 0.002809015120696756 1107 7 -0.058281430434426795 1107 8 -0.010123178898474805 1107 9 0.048005952605947755 1107 10-11 -0.008061049657534625 1107 12-13 -0.043657637685683426 1107 14-15 -0.10506154598056838 1107 16-17 -0.09030262930411226 1107 18-19 -0.031020669283847724 1107 20-21 -0.05925237816742879 1107 22-23 -0.04909332898437668 1107 24-25 -0.05430183792736187 1107 26-27 -0.01104108113989355 1107 28-29 0.017855108678659803 1107 30-31 0.06047542669675465 1107 32-33 -0.025625754357690766 1107 34-35 7.325485384370722E-4 1107 36-37 -0.0024944420111978616 1107 38-39 0.027227177057923768 1107 40-41 0.04255821455812736 1107 42-43 0.027794679206252226 1107 44-45 0.11872470033974736 1107 46-47 0.04218597390327261 1107 48-49 0.03081521216780203 1107 50-51 0.047529410880606804 1107 52-53 -0.008233031355352693 1107 54-55 -0.013607682951452205 1107 56-57 -0.020777004125719145 1107 58-59 -0.043378410242873144 1107 60-61 -0.03632082084793353 1107 62-63 -0.0050912525324022795 1107 64-65 0.04209481537495208 1107 66-67 0.08571236295556162 1107 68-69 0.007049740459727616 1107 70-71 0.08660980906390137 1107 72-73 0.08152612721618624 1107 74-75 0.030395626424621014 1107 76-77 0.041958415725176224 1107 78-79 0.08842215103911144 1107 80-81 0.0901747997985396 1107 82-83 0.05353267743972623 1107 84-85 0.06789527194095513 1107 86-87 0.04209609424732719 1107 88-89 0.08077906431062587 1107 90-91 0.007670656250162722 1107 92-93 0.05348265493687876 1107 94-95 0.022394263398524572 1107 96-97 0.045997323507226184 1107 98-99 0.03348979340301739 1107 100 0.04638687678752973 1108 1 -0.10585724314545075 1108 2 -0.12111918619250517 1108 3 -0.11556463861738564 1108 4 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2214 34-35 -0.12784505863930917 2214 36-37 -0.17526280694188756 2214 38-39 -0.13702148166763806 2214 40-41 -0.10962689398598258 2214 42-43 -0.15645652968309065 2214 44-45 -0.12406529304767133 2214 46-47 -0.04592662395582181 2214 48-49 -0.08349319075107786 2214 50-51 -0.08261041128985624 2214 52-53 -0.10323913707602372 2214 54-55 -0.15489369661612074 2214 56-57 -0.1342455995131857 2214 58-59 -0.12077192110562152 2214 60-61 -0.08158651973619868 2214 62-63 -0.09336578870937728 2214 64-65 -0.129768799965305 2214 66-67 -0.11475429033921358 2214 68-69 -0.1322141909041008 2214 70-71 -0.05922923483559828 2214 72-73 -0.0560580848774066 2214 74-75 -0.036294240980204506 2214 76-77 -0.037873944561027884 2214 78-79 -0.10923636260638858 2214 80-81 -0.04301135932301747 2214 82-83 -0.1548574072500557 2214 84-85 -0.16594355487366386 2214 86-87 -0.12010732729049778 2214 88-89 -0.15914532946067084 2214 90-91 -0.2342877203084015 2214 92-93 -0.2509534837600178 2214 94-95 -0.2830311787827 2214 96-97 -0.2377796958073226 2214 98-99 -0.24071489979869298 2214 100 -0.2206905088035711 2215 1 -0.16621401795659452 2215 2 -0.11611902125410722 2215 3 -0.08423215705706255 2215 4 -0.189016804284158 2215 5 -0.13511240310334927 2215 6 -0.1496466908253069 2215 7 -0.10473676387078967 2215 8 -0.1345734690649465 2215 9 -0.15065440291393628 2215 10-11 -0.18659037723048755 2215 12-13 -0.185039128932317 2215 14-15 -0.2814079280721984 2215 16-17 -0.14917947617184524 2215 18-19 -0.19489245003681077 2215 20-21 -0.1660960191588643 2215 22-23 -0.14439734180416508 2215 24-25 -0.06613164069806032 2215 26-27 -0.17916152044760736 2215 28-29 -0.14882557520090245 2215 30-31 -0.10039664339749521 2215 32-33 -0.16432600141564535 2215 34-35 -0.14136010116031628 2215 36-37 -0.2659042807369758 2215 38-39 -0.18474824348488283 2215 40-41 -0.12719935256210135 2215 42-43 -0.22798993120289168 2215 44-45 -0.18977093795616184 2215 46-47 -0.13081702853088473 2215 48-49 -0.07896710464863332 2215 50-51 -0.09086096702154833 2215 52-53 -0.23891685807154772 2215 54-55 -0.10401069821450193 2215 56-57 -0.08238048005848242 2215 58-59 -0.1067639895022694 2215 60-61 -0.09013968279442253 2215 62-63 -0.03334702698050762 2215 64-65 -0.0786131228443736 2215 66-67 -0.06091395744353889 2215 68-69 -0.007844669481187339 2215 70-71 -0.03166726395993891 2215 72-73 0.0703954303979728 2215 74-75 0.026676668115051427 2215 76-77 -0.008132260534281954 2215 78-79 0.02323940790971335 2215 80-81 -0.012741819748882222 2215 82-83 0.027251114626039907 2215 84-85 -0.007221515357755948 2215 86-87 -0.04355024858527656 2215 88-89 0.014429098581999256 2215 90-91 2.684093390357134E-4 2215 92-93 -0.07009211157592077 2215 94-95 -0.1331858447495904 2215 96-97 -0.24410847670842273 2215 98-99 -0.32999040509210076 2215 100 -0.23599522232783698 2216 1 -0.057581955778733374 2216 2 -0.026337598248861838 2216 3 -0.10404155872898357 2216 4 -0.02543116680922708 2216 5 0.01032288321339081 2216 6 -0.028623566108045395 2216 7 -0.041131614036295616 2216 8 -0.06833122240672651 2216 9 -0.15629220352533224 2216 10-11 -0.08969551356631911 2216 12-13 -0.10237507476966101 2216 14-15 -0.12217039709133104 2216 16-17 -0.18752219119811997 2216 18-19 -0.18103110406395473 2216 20-21 -0.1706198225948441 2216 22-23 -0.15958566392836815 2216 24-25 -0.18004275318681806 2216 26-27 -0.2457060229831427 2216 28-29 -0.2209430990281973 2216 30-31 -0.2510610563818716 2216 32-33 -0.21933157158007077 2216 34-35 -0.24214904470889564 2216 36-37 -0.25488265444322167 2216 38-39 -0.2293194508128451 2216 40-41 -0.24952899116492233 2216 42-43 -0.20388181101875347 2216 44-45 -0.16411607760023372 2216 46-47 -0.09473284055572861 2216 48-49 -0.07382104121003863 2216 50-51 -0.15749213957294472 2216 52-53 -0.1494638312203378 2216 54-55 -0.08903303386680506 2216 56-57 -0.16253131802576348 2216 58-59 -0.16388627928765231 2216 60-61 -0.09929410055118382 2216 62-63 -0.031451178413554715 2216 64-65 0.01267475859475553 2216 66-67 0.03439488421224013 2216 68-69 0.0012166146120549115 2216 70-71 -0.07017246294672219 2216 72-73 0.07214936440249176 2216 74-75 0.10193763163993452 2216 76-77 -0.006830603312153016 2216 78-79 -0.02265724000690028 2216 80-81 0.016854972910579136 2216 82-83 0.07962112352944928 2216 84-85 0.05350006198368362 2216 86-87 -0.04105049534663152 2216 88-89 -0.052448406795690516 2216 90-91 -0.07796490362964192 2216 92-93 -0.06857822530383473 2216 94-95 -0.1164736730511109 2216 96-97 -0.1804622952100523 2216 98-99 -0.08365172223922102 2216 100 -0.03644992029979477 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 32257.0 3 5755.0 4 3711.0 5 2999.0 6 2634.0 7 2994.0 8 3419.0 9 3856.0 10 4045.0 11 4610.0 12 5140.0 13 5652.0 14 6460.0 15 6841.0 16 6498.0 17 6724.0 18 6631.0 19 6548.0 20 6533.0 21 6707.0 22 7952.0 23 10774.0 24 16667.0 25 22185.0 26 21777.0 27 19197.0 28 20942.0 29 26417.0 30 32338.0 31 39134.0 32 51593.0 33 70117.0 34 96850.0 35 145016.0 36 267703.0 37 585373.0 38 1016831.0 39 469970.0 40 1042.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.65601823116656 20.078413478228853 13.550422378152055 23.715145912452527 2 15.052194785737461 20.31530433590209 41.00842668339297 23.624074194967477 3 17.814891794138855 25.80044926343028 30.179374211649566 26.205284730781297 4 11.86609351347843 16.9266922037574 40.023489490698275 31.183724792065888 5 12.512849897463063 38.44636872387406 36.27339205823888 12.767389320423995 6 30.2852731122866 39.01347554497605 18.05264906760449 12.648602275132864 7 26.785885736212656 32.92749303419446 23.014798670770617 17.271822558822265 8 24.35427667681603 37.329206734038515 20.780861912622406 17.535654676523052 9 24.49493848810416 17.286876249647285 21.573061987749075 36.645123274499475 10-11 23.245013622227912 27.412058547558832 29.50900987558267 19.83391795463059 12-13 23.920056302071426 24.7426957648094 29.458420281199025 21.878827651920144 14-15 21.26959427949125 26.80662607899422 26.373553625836806 25.55022601567772 16-17 21.028058823375186 30.16831492371786 26.930191157616544 21.873435095290407 18-19 20.928111862260447 29.225772156611356 28.840316531446675 21.005799449681515 20-21 22.132225603081316 28.507530240917895 28.259641238556828 21.100602917443968 22-23 21.721071127716133 28.34361188292858 28.279346710604685 21.655970278750594 24-25 21.647309760695187 28.354976262232885 28.274416486502385 21.72329749056954 26-27 21.408268653400004 28.334677566039733 28.337151915556184 21.91990186500408 28-29 21.392521468570497 28.361339236350418 28.38604984529651 21.860089449782567 30-31 21.61826593740301 28.8393263585944 28.054250372874222 21.48815733112837 32-33 21.340864279103183 28.453884837949307 28.60309116842732 21.602159714520194 34-35 21.535085577656968 28.232466714884353 28.59247718863133 21.639970518827347 36-37 21.807736628696976 28.68666786494497 28.01292305463969 21.49267245171837 38-39 21.561280362366585 28.714772805662008 28.18901420631294 21.534932625658467 40-41 21.332790669324933 28.419110948574065 28.47848940166705 21.769608980433947 42-43 21.406767011535592 28.21534632579013 28.532618090094104 21.845268572580174 44-45 21.51020719807604 28.48531976939469 28.402511321032332 21.60196171149694 46-47 21.560730416893527 28.6314194480404 28.310577098423167 21.49727303664291 48-49 21.417192769234727 28.51139774664862 28.307990899038604 21.76341858507805 50-51 21.467464366085377 28.421785615687035 28.316467009700354 21.794283008527234 52-53 21.49886660511572 28.404555207021726 28.37812707621804 21.718451111644516 54-55 21.588459896929425 28.750349180031083 28.177554666642635 21.483636256396853 56-57 21.499212350733707 28.493668905507484 28.168336581413772 21.83878216234504 58-59 21.345621594327156 28.148439536450848 28.544320461209548 21.96161840801245 60-61 21.664687766596504 28.53289367727911 28.17261207764191 21.62980647848247 62-63 21.474803960536192 28.49878547445138 28.214413156262403 21.811997408750024 64-65 21.46636839773618 28.278440621895307 28.333145468033628 21.922045512334886 66-67 21.311269944096075 28.33517320517471 28.227172891921377 22.126383958807843 68-69 21.748613771648657 28.487721696595393 28.06297320340808 21.700691328347872 70-71 21.65861274924615 28.310856363932757 28.190289261106667 21.84024162571443 72-73 21.459391233029507 28.339336813418527 28.308093377079608 21.893178576472366 74-75 21.56343222938151 28.455998773192686 28.14121980821961 21.839349189206192 76-77 21.56346429157736 28.428179948338467 28.060166060082175 21.948189700002 78-79 21.73594484942528 28.514162423665713 28.114020277604247 21.63587244930476 80-81 21.65076534578635 28.346441212134323 28.193465442663907 21.809327999415427 82-83 21.5100051641248 28.40650378265588 28.319604931345978 21.763886121873348 84-85 21.522892689045563 28.30176839665981 28.205743225973883 21.969595688320744 86-87 21.688057485781826 28.42106608345098 28.094375298899948 21.796501131867245 88-89 21.469699998895702 28.335259808831438 28.191915527579308 22.003124664693548 90-91 21.513266295808627 28.24979906544829 28.20563961802985 22.031295020713237 92-93 21.664994736965294 28.46380373841943 28.008245475982395 21.86295604863288 94-95 21.40159814886995 28.205852517739583 28.26704594616198 22.125503387228488 96-97 21.448431700124708 28.173198990268055 28.28520098639545 22.09316832321179 98-99 21.53651815937534 28.283025211191937 28.127222747752946 22.05323388167978 100 21.617540711598462 28.186539973934554 28.077373626438824 22.118545688028156 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 462.0 1 424.5 2 357.0 3 292.0 4 248.0 5 217.5 6 194.0 7 199.0 8 203.5 9 202.5 10 205.5 11 217.0 12 234.0 13 254.0 14 292.5 15 367.0 16 511.0 17 730.5 18 1010.0 19 1445.0 20 2100.5 21 3026.0 22 4615.0 23 7292.5 24 10595.5 25 14414.5 26 19801.5 27 26926.0 28 35962.5 29 45729.0 30 54555.5 31 63872.5 32 73755.5 33 83287.0 34 91817.5 35 99240.5 36 104837.0 37 109646.5 38 113245.0 39 114408.5 40 115316.5 41 115144.5 42 112795.0 43 110712.0 44 108818.5 45 105028.0 46 102688.5 47 100354.0 48 96582.5 49 93520.0 50 89488.5 51 85274.5 52 79853.5 53 74737.0 54 71436.0 55 67783.0 56 63012.5 57 57117.5 58 51341.0 59 46041.5 60 40716.0 61 35297.0 62 30465.0 63 25729.0 64 21157.5 65 17000.5 66 13090.5 67 9403.0 68 6768.5 69 5141.0 70 3645.5 71 2531.0 72 1876.5 73 1387.5 74 1010.5 75 740.5 76 530.0 77 419.0 78 323.5 79 210.0 80 127.5 81 82.5 82 48.5 83 31.5 84 25.5 85 23.0 86 16.0 87 11.5 88 11.5 89 8.0 90 8.5 91 9.5 92 7.5 93 6.5 94 4.5 95 3.0 96 1.5 97 3.5 98 3.5 99 2.0 100 3.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.329893718388462 2 0.11792684669051198 3 0.06589355062367869 4 0.03820580806922395 5 0.0420722620590768 6 0.009502302178451924 7 0.0065205452879721825 8 0.015367516281703283 9 0.017399042954337834 10-11 0.020610165759469862 12-13 0.016203063542222333 14-15 0.018185440376002823 16-17 0.019266736830792178 18-19 0.018611405646071354 20-21 0.023854055123837932 22-23 0.015482199239029429 24-25 0.02429640367352449 26-27 0.019168437153084052 28-29 0.01870970532377948 30-31 0.019496102745444464 32-33 0.02450938630855876 34-35 0.014843251333926628 36-37 0.009551452017305987 38-39 0.012991940737090302 40-41 0.009174636586091514 42-43 0.00817525652939226 44-45 0.008879737552967143 46-47 0.007782057818559765 48-49 0.0041449697433592015 50-51 0.006536928567590203 52-53 0.0071922597523110246 54-55 0.0032602726439860913 56-57 0.00293260705162568 58-59 0.0029653736108617213 60-61 0.0036862379140546256 62-63 0.0027196244165914128 64-65 0.0019659935541624673 66-67 0.001605561402566015 68-69 0.0028179240942995364 70-71 0.0021134430707246523 72-73 0.0016710945210380971 74-75 0.002228126028050796 76-77 0.003440488719784318 78-79 0.0033258057624581732 80-81 0.0033258057624581732 82-83 0.002228126028050796 84-85 0.0028834572127716183 86-87 0.0025066417815571454 88-89 0.599185685469866 90-91 0.6083439387763394 92-93 0.6052311156489155 94-95 0.5920753421156449 96-97 0.5903714810353708 98-99 0.5872422746283289 100 0.5894704006563797 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3051892.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.75062014513422 #Duplication Level Percentage of deduplicated Percentage of total 1 75.7029276539739 48.261085847430664 2 14.493599689157847 18.479519366382743 3 4.706387317718145 9.001053304431798 4 1.9538886551240104 4.982464538347918 5 1.0313474332126673 3.2874519226199967 6 0.5767999290017696 2.2062811904119317 7 0.36025671128847936 1.6076612129260994 8 0.24268701633594347 1.2377158234070973 9 0.17015457958174585 0.9762713971983802 >10 0.72367420303055 7.836402745934319 >50 0.030160880308838314 1.29634356657632 >100 0.008064473526034574 0.8055075462510348 >500 5.145774011971178E-5 0.022241538081633722 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 6.553311847208224E-5 0.0 4 0.0 0.0 0.0 6.553311847208224E-5 0.0 5 0.0 0.0 0.0 6.553311847208224E-5 0.0 6 0.0 0.0 0.0 6.553311847208224E-5 0.0 7 0.0 0.0 0.0 6.553311847208224E-5 0.0 8 0.0 0.0 0.0 9.829967770812336E-5 0.0 9 0.0 0.0 0.0 1.638327961802056E-4 0.0 10-11 0.0 0.0 0.0 1.638327961802056E-4 0.0 12-13 0.0 0.0 0.0 1.8021607579822617E-4 0.0 14-15 0.0 0.0 0.0 1.9659935541624672E-4 0.0 16-17 0.0 0.0 0.0 1.9659935541624672E-4 0.0 18-19 0.0 0.0 0.0 2.2936591465228784E-4 0.0 20-21 0.0 3.276655923604112E-5 0.0 2.785157535063495E-4 0.0 22-23 0.0 3.276655923604112E-5 0.0 3.2766559236041114E-4 0.0 24-25 0.0 3.276655923604112E-5 0.0 4.2596527006853453E-4 0.0 26-27 0.0 3.276655923604112E-5 0.0 6.717144643388429E-4 0.0 28-29 0.0 3.276655923604112E-5 0.0 0.0014744951656218504 0.0 30-31 0.0 3.276655923604112E-5 0.0 0.005160733079676476 0.0 32-33 0.0 3.276655923604112E-5 0.0 0.013942170954935496 0.0 34-35 0.0 3.276655923604112E-5 0.0 0.025623449322584153 0.0 36-37 0.0 3.276655923604112E-5 0.0 0.047020012503719004 0.0 38-39 0.0 3.276655923604112E-5 0.0 0.08325982701878049 0.0 40-41 0.0 3.276655923604112E-5 0.0 0.12479144085046259 0.0 42-43 0.0 3.276655923604112E-5 0.0 0.1669456193076295 0.0 44-45 0.0 3.276655923604112E-5 0.0 0.21565310961200462 0.0 46-47 0.0 3.276655923604112E-5 0.0 0.2788106525394739 0.0 48-49 0.0 3.276655923604112E-5 0.0 0.34796447580713863 0.0 50-51 0.0 3.276655923604112E-5 0.0 0.42534270544304975 0.0 52-53 0.0 3.276655923604112E-5 0.0 0.5017543215814977 0.0 54-55 0.0 3.276655923604112E-5 0.0 0.5829990052072616 0.0 56-57 0.0 3.276655923604112E-5 0.0 0.6791688565650422 0.0 58-59 0.0 3.276655923604112E-5 0.0 0.790575157967582 0.0 60-61 0.0 3.276655923604112E-5 0.0 0.9111724792358313 0.0 62-63 0.0 3.276655923604112E-5 0.0 1.033490044863973 0.0 64-65 0.0 3.276655923604112E-5 0.0 1.164064783419597 0.0 66-67 0.0 3.276655923604112E-5 0.0 1.3075167797549847 0.0 68-69 0.0 3.276655923604112E-5 0.0 1.4732828029301168 0.0 70-71 0.0 3.276655923604112E-5 0.0 1.6545637919035143 0.0 72-73 1.638327961802056E-5 3.276655923604112E-5 0.0 1.8433483229419652 0.0 74-75 3.276655923604112E-5 3.276655923604112E-5 0.0 2.034393746567703 0.0 76-77 3.276655923604112E-5 3.276655923604112E-5 0.0 2.2406592369585816 0.0 78-79 3.276655923604112E-5 3.276655923604112E-5 0.0 2.479494687230085 0.0 80-81 3.276655923604112E-5 3.276655923604112E-5 0.0 2.7402509656305005 0.0 82-83 3.276655923604112E-5 3.276655923604112E-5 0.0 3.009919748143119 0.0 84-85 3.276655923604112E-5 3.276655923604112E-5 0.0 3.2779829692531717 0.0 86-87 3.276655923604112E-5 3.276655923604112E-5 0.0 3.5602668770716654 0.0 88 3.276655923604112E-5 3.276655923604112E-5 0.0 3.792401565979399 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCAACGC 3490 0.0 32.170315 4 GTATCAA 3895 0.0 30.968 1 ATCAACG 3650 0.0 30.896915 3 CAACGCA 3765 0.0 29.695301 5 GTACATG 7940 0.0 27.415876 1 AACGCAG 4120 0.0 27.353876 6 GGTATCA 2605 0.0 26.769156 1 TACATGG 7755 0.0 26.434134 2 TATCAAC 4665 0.0 24.894629 2 ACATGGG 8270 0.0 24.091127 3 ACGCAGA 4860 0.0 22.802402 7 CGCAGAG 4900 0.0 22.137465 8 CATGGGG 5165 0.0 18.918026 4 GAGTACA 5760 0.0 18.814213 1 GCAGAGT 5840 0.0 17.609346 9 TGCACCG 350 1.5224941E-9 17.448215 5 GTGTACG 315 1.4713441E-7 16.453669 1 AGTACAT 6170 0.0 16.30756 2 AGAGTAC 4980 0.0 15.281339 5 TACACTG 2080 0.0 15.1316805 5 >>END_MODULE