FastQCFastQC Report
Wed 25 May 2016
SRR1033128_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033128_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3051892
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53660.17582535686059664No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43830.14361582913156823No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43100.051.104181
GTATCAA79150.041.0881
ATCAACG91000.034.8762663
TCAACGC91550.034.6667444
CAACGCA93950.033.6810725
AACGCAG97800.032.386276
TATCAAC99750.032.3359762
ACGCAGA110500.028.2387627
CGCAGAG111550.027.8035348
GTACATG82200.026.337461
TACATGG79200.025.824912
GCAGAGT125000.024.7366859
ACATGGG86300.022.7736743
GAGTACT88700.021.165212-13
AGAGTAC116850.020.75136210-11
CAGAGTA124700.020.61325310-11
GAGTACA60200.020.4047781
GTGGTAT22500.019.4530751
AGTACTT95300.019.25561112-13
CATGGGG50000.019.089464
Produced by FastQC (version 0.11.5)