FastQCFastQC Report
Wed 25 May 2016
SRR1033127_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033127_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3305298
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT41230.12473913093463888No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG138600.028.9193211
TACATGG140750.027.1540322
ACATGGG141150.025.8667343
CATGGGG102600.021.3386864
GAGTACA95300.019.5714971
CATGGGA45700.018.9160654
CGCAATC5100.018.52065394
GTATAGA13800.018.4304711
GTATCAA101500.017.9583591
AGTACAT97150.017.952432
TATACTG10750.017.4807855
GTACCGT4053.274181E-1117.3924856
GTATTAG17250.016.928731
TCAACGC107850.016.1615474
CAACGCA110900.015.7163545
GTATTAA11800.015.566981
ATCAACG112300.015.5655573
AACGCAG112600.015.5559246
TATGTCG8500.015.47563655
GGTATCA87650.015.3686561
Produced by FastQC (version 0.11.5)