##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033127_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3305298
Sequences flagged as poor quality	0
Sequence length	100
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.676368061215662	33.0	31.0	34.0	30.0	34.0
2	31.81702164222409	34.0	31.0	34.0	30.0	34.0
3	31.913152762625337	34.0	31.0	34.0	30.0	34.0
4	35.293663082723555	37.0	35.0	37.0	33.0	37.0
5	35.38737929227561	37.0	35.0	37.0	35.0	37.0
6	35.43159951084592	37.0	37.0	37.0	35.0	37.0
7	35.41981207140778	37.0	37.0	37.0	35.0	37.0
8	35.42265114976017	37.0	37.0	37.0	35.0	37.0
9	37.099933198156414	39.0	38.0	39.0	35.0	39.0
10-11	37.09231104124348	39.0	38.0	39.0	35.0	39.0
12-13	37.05616891427036	39.0	38.0	39.0	35.0	39.0
14-15	38.51071854943185	41.0	39.0	41.0	35.0	41.0
16-17	38.498098204760964	41.0	39.0	41.0	35.0	41.0
18-19	38.46851766467048	41.0	39.0	41.0	34.0	41.0
20-21	38.479415169222264	41.0	39.0	41.0	34.0	41.0
22-23	38.43452753730526	41.0	39.0	41.0	34.0	41.0
24-25	38.416394073998774	41.0	39.0	41.0	34.0	41.0
26-27	38.36796409884978	40.5	39.0	41.0	34.0	41.0
28-29	38.332463517661644	40.0	39.0	41.0	34.0	41.0
30-31	38.32218063242709	40.0	39.0	41.0	34.0	41.0
32-33	38.3912104143106	41.0	39.0	41.0	34.0	41.0
34-35	38.35224206712981	41.0	39.0	41.0	34.0	41.0
36-37	38.26615769591728	41.0	39.0	41.0	34.0	41.0
38-39	38.17485942266023	41.0	38.0	41.0	33.0	41.0
40-41	38.098067405722574	41.0	38.0	41.0	33.0	41.0
42-43	37.95619366241712	40.0	38.0	41.0	33.0	41.0
44-45	37.797824129624615	40.0	38.0	41.0	33.0	41.0
46-47	37.64100332254459	40.0	38.0	41.0	33.0	41.0
48-49	37.485978571372385	40.0	37.0	41.0	32.0	41.0
50-51	37.32394945932258	40.0	37.0	41.0	32.0	41.0
52-53	37.1522463330084	40.0	36.5	41.0	31.5	41.0
54-55	36.95117263254327	40.0	36.0	41.0	31.0	41.0
56-57	36.75136704769131	39.5	35.0	41.0	31.0	41.0
58-59	36.510757275138275	39.0	35.0	41.0	31.0	41.0
60-61	36.23178303438903	39.0	35.0	41.0	31.0	41.0
62-63	35.93251122894214	38.0	35.0	40.5	30.0	41.0
64-65	35.624145689738114	37.5	35.0	40.0	30.0	41.0
66-67	35.27787343228961	37.0	35.0	40.0	30.0	41.0
68-69	34.93425237300843	36.5	34.5	39.0	29.5	41.0
70-71	34.56530409663516	36.0	34.0	39.0	29.0	41.0
72-73	34.195802920039284	35.0	34.0	38.5	29.0	40.5
74-75	33.83159824015868	35.0	34.0	37.0	29.0	39.5
76-77	33.529598238948495	35.0	34.0	37.0	29.0	39.0
78-79	33.200518077341286	35.0	34.0	36.5	28.0	39.0
80-81	32.8868232153349	35.0	33.5	36.0	27.5	38.0
82-83	32.73397996186728	35.0	34.0	36.0	28.0	37.0
84-85	32.528663981280964	35.0	33.5	35.0	27.0	37.0
86-87	32.35029534402042	35.0	33.5	35.0	27.0	36.0
88-89	32.056486283536316	35.0	33.0	35.0	27.0	36.0
90-91	31.91209869730354	35.0	33.0	35.0	26.5	36.0
92-93	31.77084713692986	35.0	33.0	35.0	26.0	36.0
94-95	31.625937358749496	35.0	33.0	35.0	25.0	35.0
96-97	31.47137005498445	35.0	33.0	35.0	25.0	35.0
98-99	31.296186304532903	35.0	33.0	35.0	25.0	35.0
100	31.14408443656215	35.0	33.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.12741676198193375
1101	2	0.18472598368556703
1101	3	0.1738508327809427
1101	4	0.11557128475595135
1101	5	0.14914768895427244
1101	6	0.17649245932449986
1101	7	0.21123352632202597
1101	8	0.15778004597169115
1101	9	0.25992280294138226
1101	10-11	0.20489928301072524
1101	12-13	0.24106491366918448
1101	14-15	0.34450812582275603
1101	16-17	0.2914451846697048
1101	18-19	0.24453351866505102
1101	20-21	0.22300775990805022
1101	22-23	0.2586602351279197
1101	24-25	0.20507800013484
1101	26-27	0.23029099440112333
1101	28-29	0.2794429759873793
1101	30-31	0.28609399990994433
1101	32-33	0.3046837987806512
1101	34-35	0.292261019865812
1101	36-37	0.3230209368641894
1101	38-39	0.28445177687989087
1101	40-41	0.28181015412801
1101	42-43	0.2847109064358122
1101	44-45	0.22897314284089276
1101	46-47	0.29292564326988213
1101	48-49	0.20951227348809454
1101	50-51	0.24968547370161076
1101	52-53	0.2732194585954417
1101	54-55	0.22938747705828888
1101	56-57	0.25490824026675085
1101	58-59	0.21965929642660598
1101	60-61	0.17567583889288585
1101	62-63	0.20051966003167365
1101	64-65	0.1425562235453981
1101	66-67	0.13117065048491128
1101	68-69	0.16284740512018203
1101	70-71	0.13705419120587692
1101	72-73	0.22624195729584784
1101	74-75	0.16479480116391443
1101	76-77	0.15388137160132231
1101	78-79	0.15199771464661183
1101	80-81	0.16680158047679328
1101	82-83	0.17610749097178768
1101	84-85	0.134920255127696
1101	86-87	0.18759155497227908
1101	88-89	0.3039709169918581
1101	90-91	0.31344484485051183
1101	92-93	0.28923320400411257
1101	94-95	0.24934941025722424
1101	96-97	0.24525969290050043
1101	98-99	0.29777607234156633
1101	100	0.33286386525648126
1102	1	0.03877082802577547
1102	2	0.04038691705576625
1102	3	0.0044230473989728125
1102	4	0.028384843962278694
1102	5	-0.040648431427889875
1102	6	-0.04732000647085499
1102	7	0.0032783641365270455
1102	8	0.04942114093005756
1102	9	-0.028377301393462062
1102	10-11	0.028361339601850943
1102	12-13	0.03999007702660151
1102	14-15	0.06692666329171004
1102	16-17	0.02969315002643924
1102	18-19	-0.044722609158220905
1102	20-21	0.048713243233251546
1102	22-23	0.017732502652933135
1102	24-25	0.07005027801369579
1102	26-27	0.08336062340370631
1102	28-29	0.027237380590399596
1102	30-31	0.08762440549600825
1102	32-33	0.03972733610737578
1102	34-35	0.036669093130036856
1102	36-37	0.04687166066584325
1102	38-39	-0.005552728747019842
1102	40-41	0.05568940787786403
1102	42-43	0.034928792181766255
1102	44-45	0.00569131132630929
1102	46-47	-0.05071388495912288
1102	48-49	-0.04190569053744042
1102	50-51	-0.03556565242067222
1102	52-53	-0.11306622659549959
1102	54-55	-0.04182409853441271
1102	56-57	-0.05046062380326788
1102	58-59	-0.13294028532378377
1102	60-61	-0.13670590262293558
1102	62-63	-0.02268522880109458
1102	64-65	0.03331254877429046
1102	66-67	-0.07086575634589565
1102	68-69	-0.12083130272063158
1102	70-71	-0.041531337767231946
1102	72-73	0.014990064306765305
1102	74-75	-0.029936459808602933
1102	76-77	-0.10114678287796863
1102	78-79	-0.014187242475976802
1102	80-81	-0.00944935677325276
1102	82-83	-0.04946356310597366
1102	84-85	-0.09343325112614309
1102	86-87	-0.0652395374943282
1102	88-89	0.14883342141499867
1102	90-91	0.14441699074634684
1102	92-93	0.21389223841185867
1102	94-95	0.17689343967577997
1102	96-97	0.1978317118195818
1102	98-99	0.15748879880336375
1102	100	0.16598557065902142
1103	1	0.09701162201765356
1103	2	0.05991045271659701
1103	3	0.08001829769119695
1103	4	0.08437259129356534
1103	5	0.005455763613028353
1103	6	0.016947945751567772
1103	7	0.10341055343816663
1103	8	0.005656783045395741
1103	9	0.03438388403903048
1103	10-11	0.09226894733977531
1103	12-13	0.10212225904257366
1103	14-15	0.14914952452754449
1103	16-17	0.14229439232355645
1103	18-19	0.17318874523342487
1103	20-21	0.14807597513940607
1103	22-23	0.19142553386791406
1103	24-25	0.1658914377459979
1103	26-27	0.17278755113399313
1103	28-29	0.2282475609425063
1103	30-31	0.20374184678833984
1103	32-33	0.18445540545599215
1103	34-35	0.21442126482768487
1103	36-37	0.1889427387121856
1103	38-39	0.18446668299920788
1103	40-41	0.21309754132211367
1103	42-43	0.18939816119300446
1103	44-45	0.21587523796329577
1103	46-47	0.2076595992239234
1103	48-49	0.21444643116232953
1103	50-51	0.14058066743007913
1103	52-53	0.042442989088904426
1103	54-55	0.15068648731054424
1103	56-57	0.11367452438945946
1103	58-59	0.05716432317867515
1103	60-61	0.12845406555691596
1103	62-63	0.14340493677932642
1103	64-65	0.10132615293404967
1103	66-67	0.13909652667217642
1103	68-69	0.11868280255632868
1103	70-71	0.1125515494328937
1103	72-73	0.12210483585787557
1103	74-75	0.11182732250845362
1103	76-77	0.09570831387794954
1103	78-79	0.08768066566816657
1103	80-81	0.07792560867675036
1103	82-83	0.08122862524852792
1103	84-85	0.08351144110683606
1103	86-87	0.061694749896958
1103	88-89	0.15009040370127025
1103	90-91	0.17467593242141177
1103	92-93	0.23234555527958634
1103	94-95	0.2599372360905967
1103	96-97	0.17797100919906583
1103	98-99	0.20822663185505874
1103	100	0.24768834918434024
1104	1	0.15146097940852243
1104	2	0.09872299897988768
1104	3	0.11588869145479919
1104	4	0.08701663013389549
1104	5	0.057699388622211245
1104	6	0.01631943748630249
1104	7	0.03131299971210666
1104	8	0.06330420322667152
1104	9	0.02220964542506465
1104	10-11	0.05694053832078083
1104	12-13	0.09520053173851295
1104	14-15	0.002437407287729343
1104	16-17	0.04529096379597064
1104	18-19	0.01635238383411064
1104	20-21	0.01748911679212739
1104	22-23	0.017271635337252178
1104	24-25	0.08762059398952715
1104	26-27	0.10712193190761354
1104	28-29	0.14961461492908512
1104	30-31	0.06921393468424242
1104	32-33	0.06607400827753196
1104	34-35	0.04297464404082518
1104	36-37	0.1005259537665637
1104	38-39	0.04791931545086214
1104	40-41	0.07249651155110826
1104	42-43	0.046373149853884854
1104	44-45	0.035453146947347136
1104	46-47	-0.0033605955510154217
1104	48-49	-0.1230378776296206
1104	50-51	-0.11425440758232241
1104	52-53	-0.1512287326475743
1104	54-55	-0.19290877665124384
1104	56-57	-0.14398967571007404
1104	58-59	-0.1316724668692686
1104	60-61	-0.1472080631818784
1104	62-63	-0.12847862337689975
1104	64-65	-0.17655584212547382
1104	66-67	-0.1573442564230163
1104	68-69	-0.2805939190224933
1104	70-71	-0.19345067804061244
1104	72-73	-0.22079521911234679
1104	74-75	-0.19772329540487732
1104	76-77	-0.22198870077394162
1104	78-79	-0.14543405098162765
1104	80-81	-0.042761759396377386
1104	82-83	-0.09603085909863296
1104	84-85	-0.10746497594182358
1104	86-87	-0.08217243252985895
1104	88-89	0.01989473133258457
1104	90-91	-0.007382365997866458
1104	92-93	-0.01640927997182473
1104	94-95	-7.439536210398501E-4
1104	96-97	0.01661185346047489
1104	98-99	0.09512248589226147
1104	100	0.14449573270117
1105	1	0.01330557333848148
1105	2	0.029625385475110733
1105	3	0.0617494277495787
1105	4	0.027866399491699667
1105	5	0.04545593815289806
1105	6	-0.011901312890337579
1105	7	-0.01000349003971479
1105	8	-0.07812911762396624
1105	9	-0.010893721623048691
1105	10-11	0.030749534142813673
1105	12-13	-0.012187719401751451
1105	14-15	0.019470328807265957
1105	16-17	0.04681999295888062
1105	18-19	0.004351202580245683
1105	20-21	0.021356321077433904
1105	22-23	0.07188001387001464
1105	24-25	0.037409258266649204
1105	26-27	0.10104119247202448
1105	28-29	0.11700948419732526
1105	30-31	0.07050727393103529
1105	32-33	0.1447745297894727
1105	34-35	0.10712069844580441
1105	36-37	0.14598498608753374
1105	38-39	0.06737661169967168
1105	40-41	0.059139304349336896
1105	42-43	0.09014268282285798
1105	44-45	0.076418755116201
1105	46-47	0.15041499468763675
1105	48-49	0.12503031066000148
1105	50-51	0.12645439219816268
1105	52-53	0.0858568362094374
1105	54-55	0.08620393257046999
1105	56-57	0.06186225510934662
1105	58-59	0.01151603846924587
1105	60-61	0.03657955099593124
1105	62-63	0.0823826349475354
1105	64-65	0.14009233059817916
1105	66-67	0.14259834188681708
1105	68-69	0.07145176849486745
1105	70-71	0.03324641446994292
1105	72-73	0.06398164125464945
1105	74-75	0.010371550911322913
1105	76-77	-0.03745611576939467
1105	78-79	0.001856248413155015
1105	80-81	0.06428335431980514
1105	82-83	0.013283854026660435
1105	84-85	0.03334486461910302
1105	86-87	0.070954417223966
1105	88-89	0.05559146403096804
1105	90-91	0.031709889152971016
1105	92-93	0.10558313894963334
1105	94-95	0.05878276394279425
1105	96-97	0.09625940578855818
1105	98-99	0.07604169813657435
1105	100	0.0933078348153451
1106	1	0.1050006105491228
1106	2	0.11494362896838695
1106	3	0.13650313853450058
1106	4	0.10992655326827361
1106	5	0.043246325061332413
1106	6	0.05688574356293685
1106	7	0.03311829771571695
1106	8	0.07421515275964907
1106	9	0.12521231842465141
1106	10-11	0.026779919406578756
1106	12-13	0.05181098542123408
1106	14-15	0.082529824716147
1106	16-17	0.06894141066374715
1106	18-19	0.09193240812277992
1106	20-21	0.05617129579064084
1106	22-23	0.07528889919544923
1106	24-25	0.09915353276194594
1106	26-27	0.17196179606562367
1106	28-29	0.1797779764468217
1106	30-31	0.15612176346682816
1106	32-33	0.11885279548577898
1106	34-35	0.10304561929610401
1106	36-37	0.1216307243384307
1106	38-39	0.17308525854807044
1106	40-41	0.11578736569549619
1106	42-43	0.17466233661998842
1106	44-45	0.1610567223050552
1106	46-47	0.09330560362641194
1106	48-49	0.055560922065780005
1106	50-51	0.12354178580862651
1106	52-53	0.06462384825663747
1106	54-55	0.06217151202854865
1106	56-57	0.0831581516596529
1106	58-59	0.03630778222977682
1106	60-61	-0.023225761285644353
1106	62-63	0.07137763926478868
1106	64-65	0.06472289677555665
1106	66-67	0.07747159956537075
1106	68-69	0.0665748103105912
1106	70-71	0.021525370927328424
1106	72-73	0.04055692598800675
1106	74-75	0.0733088273390976
1106	76-77	0.1312646399561075
1106	78-79	0.10353823106189708
1106	80-81	0.07637475681147521
1106	82-83	0.024465240482811623
1106	84-85	0.10025381161963764
1106	86-87	0.09683688505013066
1106	88-89	0.18406109932557513
1106	90-91	0.20763563233787252
1106	92-93	0.2351861569176421
1106	94-95	0.2372261873217667
1106	96-97	0.21793707118060368
1106	98-99	0.2278243139829641
1106	100	0.27160971384992294
1107	1	0.007533719512164083
1107	2	0.017352641768773935
1107	3	0.0038242779161343776
1107	4	-0.036958073112650425
1107	5	-0.040421674174936584
1107	6	-0.07157167109374996
1107	7	-0.07582729050938752
1107	8	-0.0942172527213927
1107	9	-0.15674797357330306
1107	10-11	-0.10944333892889802
1107	12-13	-0.16145475982350632
1107	14-15	-0.15518781100661272
1107	16-17	-0.10949901793495798
1107	18-19	-0.08520981870292843
1107	20-21	-0.1232313924978854
1107	22-23	-0.1471469232829321
1107	24-25	-0.11821882200825229
1107	26-27	-0.06342375381471754
1107	28-29	-0.11971394553008707
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2216	7	-0.05626106302584333
2216	8	-0.027131534328049156
2216	9	-0.11466996032245902
2216	10-11	-0.0309075987371088
2216	12-13	-0.07964916853733683
2216	14-15	-0.12297774808383366
2216	16-17	-0.10141085216697121
2216	18-19	-0.15735560853438102
2216	20-21	-0.15111140539157475
2216	22-23	-0.14905032020053
2216	24-25	-0.20590482734897364
2216	26-27	-0.2427112671895344
2216	28-29	-0.18767835357309792
2216	30-31	-0.19778110648922365
2216	32-33	-0.15106587074043887
2216	34-35	-0.14710008077496894
2216	36-37	-0.1582406431701031
2216	38-39	-0.08831428297970234
2216	40-41	-0.11258435717905968
2216	42-43	-0.15954920126403493
2216	44-45	-0.11535558236875687
2216	46-47	2.4364981398150576E-4
2216	48-49	-0.002784373588738731
2216	50-51	0.01296789470077897
2216	52-53	0.021615950121550043
2216	54-55	0.00884743823254297
2216	56-57	-0.07386983716883577
2216	58-59	-0.1202772003022261
2216	60-61	-0.001661049632637912
2216	62-63	0.009843058646751501
2216	64-65	-0.0052151894532599385
2216	66-67	-0.027839921301122672
2216	68-69	-0.03865757905045086
2216	70-71	-0.1074909158480466
2216	72-73	0.011581080867529181
2216	74-75	0.08075604797635805
2216	76-77	-0.052296327840714696
2216	78-79	-0.03380863621139696
2216	80-81	-0.011732243398739683
2216	82-83	-0.030949937150154483
2216	84-85	-0.13796640520368442
2216	86-87	-0.2038998738115012
2216	88-89	-0.11079029761327064
2216	90-91	-0.09450262006209798
2216	92-93	-0.10583509497180188
2216	94-95	-0.13534751359016894
2216	96-97	-0.10128793057119978
2216	98-99	-0.07278015862382148
2216	100	-0.06305208780577942
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	34125.0
3	5730.0
4	3805.0
5	3295.0
6	2929.0
7	3422.0
8	3909.0
9	4099.0
10	4428.0
11	4873.0
12	5274.0
13	5848.0
14	6386.0
15	6820.0
16	6694.0
17	6654.0
18	6664.0
19	6528.0
20	6317.0
21	6866.0
22	8011.0
23	10939.0
24	16466.0
25	22133.0
26	22201.0
27	19813.0
28	22849.0
29	28702.0
30	36120.0
31	44692.0
32	59464.0
33	82448.0
34	120454.0
35	195931.0
36	389502.0
37	784049.0
38	1008811.0
39	297702.0
40	345.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.32628569728737	18.326707013457227	12.978419991512968	24.368587297742437
2	16.22004739047956	19.448997079212617	38.80558181460769	25.525373715700134
3	19.157599646401348	24.687543518669887	28.79210336705841	27.362753467870355
4	13.20647471105417	16.110899750695598	36.597020635334275	34.085604902915954
5	14.298024449966555	37.521800059929355	34.094537045724884	14.0856384443792
6	32.55453259247515	36.02542938024736	17.358205577253347	14.061832450024145
7	29.476164930998372	31.57279413700237	21.13082730244198	17.820213629557283
8	27.042870374601	34.81006395803951	19.26787001906002	18.879195648299472
9	26.56409856051824	16.683748238510184	19.444941207015034	37.30721199395654
10-11	25.751667847079606	25.865432319368452	27.124921076748425	21.257978756803514
12-13	26.045523914450857	23.259795136787037	27.100764783728348	23.59391616503376
14-15	23.416073033688313	24.653106169460877	24.47510327269635	27.455717524154455
16-17	23.606761012593495	27.884509328808445	25.029878059389972	23.47885159920809
18-19	23.737535888410648	27.26478263606126	26.210447098182943	22.787234377345154
20-21	24.64528378054091	26.161650794073495	25.97825175826128	23.21481366712432
22-23	24.234685733867607	26.036548326261556	25.896101204232323	23.832664735638513
24-25	24.108832914966342	26.255441418786113	25.90811359609504	23.727612070152503
26-27	23.76765059745139	26.40617737624913	26.011830409016966	23.814341617282516
28-29	23.844780863425623	26.499145083446844	26.073388923712937	23.58268512941459
30-31	23.992276398654514	26.5749350314524	25.63340551388396	23.79938305600912
32-33	23.835868729239568	26.318367287650663	25.997442588750204	23.848321394359562
34-35	24.084950673036527	26.220185543274393	26.008843707954604	23.686020075734483
36-37	24.18517981732488	26.118255015971286	25.70743720183371	23.98912796487012
38-39	24.04685923967204	26.56917739588756	25.73200541684387	23.65195794759653
40-41	23.85663645937295	26.408938708407103	25.937395092551537	23.797029739668407
42-43	23.82781120320965	26.396316529174424	25.83674605563935	23.939126211976575
44-45	23.754466299690563	26.193612824577333	25.94894023622788	24.102980639504224
46-47	23.99424698306243	26.23504617254482	25.955700801364944	23.81500604302781
48-49	23.93078479031106	26.29931687315885	25.851609123223483	23.918289213306608
50-51	23.74964732507891	26.4010214524685	25.909417195710567	23.939914026742027
52-53	23.922032262407775	26.33148617962927	25.835023910126363	23.911457647836592
54-55	23.841122517501518	26.428262501045698	25.86306451913016	23.867550462322633
56-57	23.74009409943704	26.26836220326168	25.922652211179443	24.068891486121828
58-59	24.00161986363072	26.03068513061096	25.929420962287946	24.038274043470377
60-61	23.806080468206822	26.29521427711314	26.11298660355815	23.785718651121897
62-63	23.656527865139505	26.38029264591224	25.941078710876692	24.022100778071557
64-65	23.7990015549554	26.39910057954953	25.761219421467036	24.040678444028035
66-67	23.69706208438299	26.15394017671575	25.833586339798952	24.315411399102306
68-69	23.80198087501959	26.269951101383697	25.941696221052485	23.98637180254423
70-71	23.869444834819795	26.151075713941886	25.979740855648643	23.99973859558968
72-73	23.845690014319672	26.15187446127259	25.94447669959516	24.057958824812577
74-75	23.68191950188073	26.436445847606787	25.8487564793097	24.032878171202782
76-77	23.82059009600353	26.091033770816875	25.899123530228664	24.189252602950933
78-79	23.890370648720356	26.24468204035984	25.920570714283876	23.944376596635927
80-81	23.99185642917308	26.061211720739298	26.02233340110566	23.924598448981964
82-83	23.75907917973561	26.15617626262886	26.150185751235846	23.934558806399682
84-85	23.707355859036756	26.271177979439685	25.8601289724501	24.161337189073468
86-87	23.804969685069327	26.232947615128133	25.97391791982348	23.98816477997906
88-89	23.61666829761456	26.234529901210212	25.940883186438956	24.207918614736272
90-91	23.779469242005426	26.175759838529412	25.943876459963473	24.10089445950169
92-93	23.940259353634076	26.269958270005755	25.961054643493007	23.828727732867158
94-95	23.865940654418445	26.03642935525553	26.01570267037921	24.081927319946818
96-97	23.850206607482914	26.085864834240752	26.059721234355308	24.00420732392103
98-99	23.48688202987524	26.39766656379972	25.991311699623655	24.124139706701385
100	23.740949171710927	25.870813625751694	25.906300207349986	24.481936995187397
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	388.0
1	377.5
2	346.5
3	310.0
4	284.5
5	253.5
6	223.5
7	220.5
8	218.0
9	215.5
10	206.0
11	216.5
12	228.0
13	219.0
14	239.5
15	289.0
16	352.0
17	420.5
18	521.0
19	691.5
20	928.0
21	1209.0
22	1763.5
23	2706.5
24	4084.5
25	5908.5
26	8219.0
27	11305.0
28	15362.5
29	20314.0
30	24982.5
31	29320.0
32	34543.5
33	41527.5
34	49114.0
35	56116.0
36	64166.5
37	73479.5
38	81122.5
39	86848.0
40	94190.0
41	102392.0
42	110149.0
43	118170.0
44	121125.5
45	122256.0
46	124709.5
47	124719.5
48	129531.5
49	131505.0
50	126225.0
51	123021.0
52	118721.5
53	116068.5
54	114623.5
55	110987.5
56	103777.0
57	95713.0
58	87698.5
59	78204.0
60	70490.5
61	62841.0
62	56114.5
63	48425.5
64	39499.5
65	32626.0
66	26708.0
67	21843.0
68	18484.5
69	15297.5
70	11610.0
71	8173.5
72	6104.0
73	4597.0
74	3225.5
75	2112.5
76	1374.0
77	1004.5
78	682.0
79	405.0
80	265.0
81	153.0
82	100.5
83	70.5
84	47.5
85	41.0
86	34.5
87	28.0
88	20.5
89	16.5
90	15.0
91	10.5
92	6.5
93	8.5
94	7.0
95	3.5
96	2.0
97	3.0
98	4.0
99	3.0
100	3.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.32856341546208545
2	0.11469465083027308
3	0.06407894235255036
4	0.03948206788011247
5	0.04259827706911752
6	0.009136846359995376
7	0.006988779831652092
8	0.014370867619198027
9	0.017154277768600592
10-11	0.02007383297965872
12-13	0.015354137508932629
14-15	0.017441695120984552
16-17	0.018621618988666072
18-19	0.018455219468864834
20-21	0.022842115899988445
22-23	0.014991084011184468
24-25	0.023689240728067485
26-27	0.017971148138533954
28-29	0.01783500307687839
30-31	0.018818272966612995
32-33	0.023734622415286004
34-35	0.013992686892377027
36-37	0.009227609734432417
38-39	0.012752254108404143
40-41	0.009121719130922537
42-43	0.007956922492313856
44-45	0.008138449241187935
46-47	0.007548487307347174
48-49	0.003887697871719887
50-51	0.0060660188582088515
52-53	0.006444199585029852
54-55	0.0025565017133099646
56-57	0.002148066528343284
58-59	0.002465738338872925
60-61	0.002783410149402565
62-63	0.002269084360926004
64-65	0.0017698858015222836
66-67	0.0014068323037741226
68-69	0.002783410149402565
70-71	0.0022539571318531642
72-73	0.001663995198012403
74-75	0.0019816670085420437
76-77	0.0032372270215877664
78-79	0.003222099792514926
80-81	0.003449008228607526
82-83	0.002148066528343284
84-85	0.0029044279819852854
86-87	0.0023749749644358846
88-89	0.6016855363722121
90-91	0.6123956145557828
92-93	0.6090524969306853
94-95	0.595362354619765
96-97	0.5929571251971835
98-99	0.5899619338407611
100	0.5914444022898994
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3305298.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.365652389626696
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.24002158628427	26.738216856333196
2	15.223273706116636	12.289947493064965
3	6.424509704400324	7.779885765048205
4	3.448814116629341	5.568545271531898
5	2.062896775932129	4.163508708647898
6	1.363855915661244	3.3031760280670683
7	0.9468076335490963	2.6752955470981514
8	0.6959596693279079	2.2474292871431896
9	0.5336803004749493	1.9388118146547
>10	2.7748406305150306	20.107514991188257
>50	0.18634599164410176	5.169372477382798
>100	0.09516727281045721	6.716319774443546
>500	0.00315139724532875	0.8367271170230294
>1k	6.752994092618637E-4	0.46524886837303264
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	4123	0.12473913093463888	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	1.5127229072840028E-5	0.0
12-13	0.0	0.0	0.0	3.0254458145680056E-5	0.0
14-15	0.0	0.0	0.0	3.0254458145680056E-5	0.0
16-17	0.0	0.0	0.0	3.0254458145680056E-5	0.0
18-19	0.0	0.0	0.0	3.0254458145680056E-5	0.0
20-21	0.0	0.0	0.0	9.076337443704017E-5	0.0
22-23	0.0	0.0	0.0	2.269084360926004E-4	0.0
24-25	0.0	0.0	0.0	4.235624140395208E-4	0.0
26-27	0.0	0.0	0.0	5.899619338407612E-4	0.0
28-29	0.0	0.0	0.0	0.001603486281721043	0.0
30-31	0.0	0.0	0.0	0.006066018858208852	0.0
32-33	0.0	0.0	0.0	0.01530875582171411	0.0
34-35	0.0	0.0	0.0	0.02833330005342937	0.0
36-37	0.0	0.0	0.0	0.05170486897096721	0.0
38-39	0.0	0.0	0.0	0.08906912478088208	0.0
40-41	0.0	0.0	0.0	0.13271118065602558	0.0
42-43	0.0	0.0	0.0	0.1771247252138839	0.0
44-45	0.0	0.0	0.0	0.2308566428806117	0.0
46-47	0.0	0.0	0.0	0.29814255779660415	0.0
48-49	0.0	0.0	0.0	0.37264416098034125	0.0
50-51	0.0	0.0	0.0	0.4508821897450699	0.0
52-53	0.0	0.0	0.0	0.5307690864787381	0.0
54-55	0.0	0.0	0.0	0.6187490507663757	0.0
56-57	0.0	0.0	0.0	0.7229605318491706	0.0
58-59	0.0	0.0	0.0	0.8388956154634166	0.0
60-61	0.0	0.0	0.0	0.9616379521604406	0.0
62-63	0.0	0.0	0.0	1.0873754802138869	0.0
64-65	0.0	0.0	0.0	1.2201017880989853	0.0
66-67	0.0	0.0	0.0	1.3711471703912932	0.0
68-69	0.0	0.0	0.0	1.542221003976041	0.0
70-71	0.0	0.0	0.0	1.7258958193784646	0.0
72-73	0.0	0.0	0.0	1.9199327866957834	0.0
74-75	0.0	0.0	0.0	2.116859054766015	0.0
76-77	0.0	0.0	0.0	2.3320438883271644	0.0
78-79	0.0	0.0	0.0	2.5732778103517444	0.0
80-81	0.0	0.0	0.0	2.836385705615651	0.0
82-83	0.0	0.0	0.0	3.109084264111738	0.0
84-85	0.0	0.0	0.0	3.3825996929777586	0.0
86-87	0.0	0.0	0.0	3.6717718039341687	0.0
88	0.0	0.0	0.0	3.913051107645967	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACATG	13860	0.0	28.919321	1
TACATGG	14075	0.0	27.154032	2
ACATGGG	14115	0.0	25.866734	3
CATGGGG	10260	0.0	21.338686	4
GAGTACA	9530	0.0	19.571497	1
CATGGGA	4570	0.0	18.916065	4
CGCAATC	510	0.0	18.520653	94
GTATAGA	1380	0.0	18.430471	1
GTATCAA	10150	0.0	17.958359	1
AGTACAT	9715	0.0	17.95243	2
TATACTG	1075	0.0	17.480785	5
GTACCGT	405	3.274181E-11	17.392485	6
GTATTAG	1725	0.0	16.92873	1
TCAACGC	10785	0.0	16.161547	4
CAACGCA	11090	0.0	15.716354	5
GTATTAA	1180	0.0	15.56698	1
ATCAACG	11230	0.0	15.565557	3
AACGCAG	11260	0.0	15.555924	6
TATGTCG	850	0.0	15.4756365	5
GGTATCA	8765	0.0	15.368656	1
>>END_MODULE