FastQCFastQC Report
Wed 25 May 2016
SRR1033127_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033127_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3305298
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55860.1690014032017688No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45060.13632658840443435No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT43580.1318489285988737No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA136400.027.2484281
GTACATG137600.025.5063971
TACATGG137200.024.3610362
GGTATCA101700.024.3328781
TCAACGC153700.023.9173554
ACATGGG135550.023.7212123
ATCAACG155000.023.7167593
CAACGCA155550.023.5724585
AACGCAG158950.023.1196866
TATCAAC161700.022.850352
CCGTTCG1652.669667E-622.7846189
ACGCAGA179550.020.2577517
CGCAGAG182950.019.855598
GAGTACT77300.019.78824212-13
GTATATA14000.019.4907571
AGAGTAC140850.019.26774210-11
TATACTG9050.019.2191165
TATAACG1751.0769266E-418.803572
GAGTACA96300.018.7111821
AGTACTT78900.018.70201312-13
Produced by FastQC (version 0.11.5)