##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033127_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3305298
Sequences flagged as poor quality	0
Sequence length	100
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.738655334556825	34.0	31.0	34.0	31.0	34.0
2	32.83506298070552	34.0	31.0	34.0	31.0	34.0
3	32.93325866533063	34.0	31.0	34.0	31.0	34.0
4	36.35957695796264	37.0	37.0	37.0	35.0	37.0
5	36.29692269804417	37.0	37.0	37.0	35.0	37.0
6	36.38095385045463	37.0	37.0	37.0	35.0	37.0
7	36.356602037093175	37.0	37.0	37.0	35.0	37.0
8	36.35726975298445	37.0	37.0	37.0	35.0	37.0
9	38.179522391021926	39.0	39.0	39.0	37.0	39.0
10-11	38.15564421120274	39.0	39.0	39.0	37.0	39.0
12-13	38.116568460695525	39.0	39.0	39.0	37.0	39.0
14-15	39.646791000387864	41.0	40.0	41.0	37.0	41.0
16-17	39.65043636004984	41.0	40.0	41.0	37.0	41.0
18-19	39.6550069010419	41.0	40.0	41.0	37.0	41.0
20-21	39.63511883043526	41.0	40.0	41.0	37.0	41.0
22-23	39.571747388586445	41.0	40.0	41.0	37.0	41.0
24-25	39.51572868770077	41.0	39.5	41.0	36.5	41.0
26-27	39.410196145703054	41.0	39.0	41.0	36.0	41.0
28-29	39.32419936114686	41.0	39.0	41.0	36.0	41.0
30-31	39.23824311756459	41.0	39.0	41.0	36.0	41.0
32-33	39.29116693865424	41.0	39.0	41.0	35.5	41.0
34-35	39.233371695986264	41.0	39.0	41.0	35.0	41.0
36-37	39.1558537233254	41.0	39.0	41.0	35.0	41.0
38-39	39.06992440621087	41.0	39.0	41.0	35.0	41.0
40-41	38.958437181760914	41.0	39.0	41.0	35.0	41.0
42-43	38.83214902256922	41.0	39.0	41.0	35.0	41.0
44-45	38.676371994295216	41.0	38.0	41.0	35.0	41.0
46-47	38.45768414829767	41.0	38.0	41.0	35.0	41.0
48-49	38.2542421288489	40.0	38.0	41.0	34.0	41.0
50-51	38.094895377058286	40.0	37.5	41.0	34.0	41.0
52-53	37.93118653749223	40.0	37.0	41.0	34.0	41.0
54-55	37.75106904127858	40.0	37.0	41.0	34.0	41.0
56-57	37.547703414336624	40.0	36.0	41.0	33.5	41.0
58-59	37.3106763747172	39.5	35.5	41.0	33.0	41.0
60-61	37.06187611525496	39.0	35.0	41.0	33.0	41.0
62-63	36.78560783324227	39.0	35.0	41.0	33.0	41.0
64-65	36.49089053392463	38.0	35.0	41.0	32.5	41.0
66-67	36.162597139501486	37.0	35.0	40.0	32.0	41.0
68-69	35.810327389542486	37.0	35.0	39.5	32.0	41.0
70-71	35.45361341095417	36.0	35.0	39.0	31.5	41.0
72-73	35.095911170490524	36.0	35.0	39.0	31.0	41.0
74-75	34.76918329300414	35.0	35.0	37.5	31.0	40.0
76-77	34.46172311846012	35.0	35.0	37.0	31.0	39.0
78-79	34.166498905696244	35.0	34.5	36.5	31.0	39.0
80-81	33.884083976694384	35.0	34.0	36.0	31.0	38.5
82-83	33.626540783917214	35.0	34.0	36.0	30.0	37.0
84-85	33.41831825753684	35.0	34.0	35.5	30.0	37.0
86-87	33.254720603104474	35.0	34.0	35.0	30.0	36.5
88-89	33.09455849366684	35.0	34.0	35.0	30.0	36.0
90-91	32.9422033958814	35.0	34.0	35.0	30.0	36.0
92-93	32.81777815495002	35.0	34.0	35.0	30.0	36.0
94-95	32.69355153453637	35.0	34.0	35.0	29.0	35.5
96-97	32.597196833689424	35.0	34.0	35.0	29.0	35.0
98-99	32.5180272701584	35.0	34.0	35.0	29.0	35.0
100	32.43008981338445	35.0	34.0	35.0	29.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.1074090441624449
1101	2	0.1414825459284117
1101	3	0.12538682040292315
1101	4	0.0834173125120472
1101	5	0.0866254721556814
1101	6	0.08783464915698858
1101	7	0.11869542608837946
1101	8	0.12863437306207004
1101	9	0.14588904798407043
1101	10-11	0.14470756268308094
1101	12-13	0.15249198811602582
1101	14-15	0.1897054453859468
1101	16-17	0.19449984400382192
1101	18-19	0.20258733724406852
1101	20-21	0.21685887344500543
1101	22-23	0.25591569973489925
1101	24-25	0.2505736347964529
1101	26-27	0.23841225281267953
1101	28-29	0.26623161802317696
1101	30-31	0.31783448921844837
1101	32-33	0.24583606643899714
1101	34-35	0.2775088531161103
1101	36-37	0.38547622397171466
1101	38-39	0.37325329769106474
1101	40-41	0.36289089985542944
1101	42-43	0.34533697285426257
1101	44-45	0.29886588543551795
1101	46-47	0.29658475175352805
1101	48-49	0.2498853937346439
1101	50-51	0.2807894322311313
1101	52-53	0.34692159411184775
1101	54-55	0.24476760444792944
1101	56-57	0.29081292647165924
1101	58-59	0.2628366215578595
1101	60-61	0.25302622038466893
1101	62-63	0.19001893705956263
1101	64-65	0.2107208086566743
1101	66-67	0.23086818162796163
1101	68-69	0.3355887663470227
1101	70-71	0.2620986259200109
1101	72-73	0.26964900156566074
1101	74-75	0.3007350635152477
1101	76-77	0.290374444528517
1101	78-79	0.24288722962945286
1101	80-81	0.2219751852599856
1101	82-83	0.246750560286344
1101	84-85	0.2841336552421794
1101	86-87	0.28389528194790614
1101	88-89	0.31367368515271465
1101	90-91	0.30679742378738695
1101	92-93	0.2787796813116046
1101	94-95	0.3194294381557299
1101	96-97	0.3322031369197802
1101	98-99	0.3532428402038619
1101	100	0.36252012003473055
1102	1	0.13417485737690527
1102	2	0.1326914534162853
1102	3	0.10572448073099139
1102	4	0.06024604521345367
1102	5	0.03523202738772113
1102	6	0.051201968115549334
1102	7	0.11487190102697298
1102	8	0.11533907586704828
1102	9	0.1240654865135511
1102	10-11	0.12976617565541915
1102	12-13	0.1486562318704472
1102	14-15	0.14249663005862345
1102	16-17	0.09394144777945002
1102	18-19	0.1390137663868245
1102	20-21	0.18218872857089963
1102	22-23	0.1561395304052553
1102	24-25	0.15523055861380897
1102	26-27	0.17017689410902648
1102	28-29	0.09771761587731476
1102	30-31	0.10313894899263687
1102	32-33	0.1392570831405351
1102	34-35	0.16475795739520294
1102	36-37	0.09383970859626345
1102	38-39	0.08679867993548385
1102	40-41	0.041619078569532064
1102	42-43	0.053329681930712525
1102	44-45	0.08323537073591325
1102	46-47	0.11758165584170399
1102	48-49	0.09163961292428979
1102	50-51	0.0058115052518701305
1102	52-53	-0.016905076923912077
1102	54-55	0.04146982119361553
1102	56-57	0.09650150800701596
1102	58-59	0.035830110016739525
1102	60-61	0.10298927083062637
1102	62-63	0.03643067740346595
1102	64-65	-0.017565994976500576
1102	66-67	0.038699638185640595
1102	68-69	0.02368503657540799
1102	70-71	0.08752136977853553
1102	72-73	0.11001483912870924
1102	74-75	0.095778390351839
1102	76-77	0.0562027346317322
1102	78-79	0.035773459136535735
1102	80-81	-0.0035536285654629296
1102	82-83	0.017567182408740223
1102	84-85	0.04321872284365469
1102	86-87	0.046979804550559834
1102	88-89	0.07178367137414199
1102	90-91	0.08020634583890285
1102	92-93	0.08406491821541806
1102	94-95	0.0673976393524569
1102	96-97	0.019879422417233172
1102	98-99	0.1094397838869412
1102	100	0.1058498579688063
1103	1	0.09480116054348287
1103	2	0.09217932574227916
1103	3	0.07493917245852799
1103	4	0.04285042490047175
1103	5	0.011762529511265996
1103	6	0.0799905940981489
1103	7	0.06571369142473316
1103	8	0.05074609187229129
1103	9	0.10369567521685497
1103	10-11	0.0827651606710873
1103	12-13	0.10126759668969498
1103	14-15	0.15795139449710405
1103	16-17	0.16012603103707335
1103	18-19	0.12586027061045968
1103	20-21	0.08725984805445108
1103	22-23	0.0900410773798086
1103	24-25	0.10804724260670184
1103	26-27	0.1282587012091838
1103	28-29	0.09761914554841411
1103	30-31	0.12278877711922576
1103	32-33	0.131149707906431
1103	34-35	0.1734738223426362
1103	36-37	0.17488347586841257
1103	38-39	0.11530466761450953
1103	40-41	0.18579837789792464
1103	42-43	0.1746830353633797
1103	44-45	0.22512626515614187
1103	46-47	0.17982549068840825
1103	48-49	0.2231529869025337
1103	50-51	0.14161659183062625
1103	52-53	0.178724821263458
1103	54-55	0.167914648439087
1103	56-57	0.18871399627594343
1103	58-59	0.26561942221799484
1103	60-61	0.19419494836795792
1103	62-63	0.11196304329143913
1103	64-65	0.14164181179670976
1103	66-67	0.20617633430005355
1103	68-69	0.1893762208740739
1103	70-71	0.1909290806541648
1103	72-73	0.19965095654267628
1103	74-75	0.18547247434554492
1103	76-77	0.1450710794604717
1103	78-79	0.23028951560591082
1103	80-81	0.2150351561535757
1103	82-83	0.19499033798548737
1103	84-85	0.2543062086650636
1103	86-87	0.19351228291099432
1103	88-89	0.13717559694378423
1103	90-91	0.056373156625141974
1103	92-93	0.10243383519662075
1103	94-95	0.08286909251950902
1103	96-97	0.0759688730916821
1103	98-99	0.07377116693857033
1103	100	0.10178409208366901
1104	1	0.019955625093203366
1104	2	0.0500825459651395
1104	3	0.07076456693544486
1104	4	0.06225105327644087
1104	5	-0.014166699805386429
1104	6	0.033150367364186195
1104	7	0.03848899067947542
1104	8	0.06711857342375538
1104	9	0.05549100519581884
1104	10-11	0.0749067346715151
1104	12-13	0.07299279498930389
1104	14-15	0.10602866061844907
1104	16-17	0.10515052067729869
1104	18-19	0.11127073806017762
1104	20-21	0.09285982053626896
1104	22-23	0.09275565361631521
1104	24-25	0.11537146745055082
1104	26-27	0.06744494793836253
1104	28-29	0.08965596082136074
1104	30-31	0.050744032728538
1104	32-33	0.05117582372137974
1104	34-35	0.07665918443276354
1104	36-37	0.06379680086544681
1104	38-39	0.10319009583206196
1104	40-41	0.0765746927676858
1104	42-43	0.06119742739562639
1104	44-45	0.06407496910039612
1104	46-47	0.01569418661534172
1104	48-49	-0.03609170068780543
1104	50-51	0.02123796543340717
1104	52-53	0.06444747016207941
1104	54-55	-0.04761824185442265
1104	56-57	-0.049919637692077856
1104	58-59	-0.031700266976464775
1104	60-61	-0.01717400851867268
1104	62-63	-0.026080471159914964
1104	64-65	-0.05179027242127887
1104	66-67	0.0011787851383147085
1104	68-69	0.027845583631865622
1104	70-71	0.0335746365225873
1104	72-73	0.02198337399970285
1104	74-75	0.055000716094554036
1104	76-77	-0.013376749436396551
1104	78-79	-0.032432539931875226
1104	80-81	-0.001132482506207566
1104	82-83	0.04268788403820167
1104	84-85	-0.021640208491639612
1104	86-87	0.021825561559289497
1104	88-89	0.052356086904161714
1104	90-91	0.08526546372719679
1104	92-93	0.08733056372996373
1104	94-95	0.08040932408238888
1104	96-97	0.04934582392255038
1104	98-99	0.08685037454716849
1104	100	0.08007136296115647
1105	1	0.020142822337575694
1105	2	0.002635572960549837
1105	3	-0.01058339991006818
1105	4	-0.0013548514397712097
1105	5	0.019118730476471058
1105	6	0.005976308009849163
1105	7	-0.06867873529034085
1105	8	-0.06287565405001772
1105	9	0.02831888727406806
1105	10-11	-0.010005616118604621
1105	12-13	0.02376207686633336
1105	14-15	-0.004520242774141536
1105	16-17	-0.015823465851745766
1105	18-19	-0.00902752207108648
1105	20-21	0.04662783918782765
1105	22-23	0.06938570198818184
1105	24-25	0.03998192978762205
1105	26-27	0.020960752925347492
1105	28-29	0.005685614694129981
1105	30-31	0.06939943103284207
1105	32-33	0.039485077538238045
1105	34-35	0.08431994799389031
1105	36-37	-0.02644564523607329
1105	38-39	0.005093648912563253
1105	40-41	0.06335200527276186
1105	42-43	0.04226923624131018
1105	44-45	0.02610063397633411
1105	46-47	0.050987468050593066
1105	48-49	-0.03832311858936066
1105	50-51	0.016470087427855162
1105	52-53	0.02651983727749041
1105	54-55	0.07720016948422881
1105	56-57	0.09545668960067388
1105	58-59	0.029367818632948683
1105	60-61	-0.002018409168236701
1105	62-63	-0.052593472718236
1105	64-65	-0.10107649394478102
1105	66-67	-0.16475645039628972
1105	68-69	-0.19401401972913135
1105	70-71	-0.12501404552943285
1105	72-73	-0.11145730617473504
1105	74-75	-0.1230505256457306
1105	76-77	-0.14093365929696944
1105	78-79	-0.0941154125273016
1105	80-81	-0.019491229911153596
1105	82-83	-0.017150109883814935
1105	84-85	-0.08483817507829627
1105	86-87	-0.11393456969881299
1105	88-89	-0.09643347302052518
1105	90-91	-0.06557032300168686
1105	92-93	-0.039784195644571696
1105	94-95	-0.05362992133022715
1105	96-97	-0.0834138448431645
1105	98-99	-0.1870498686064721
1105	100	-0.18959996544467117
1106	1	0.05937743440122745
1106	2	0.0846671506094907
1106	3	0.05417730071901872
1106	4	0.04252533440784845
1106	5	-0.020125338435164508
1106	6	5.943335844591502E-4
1106	7	0.010080978537217788
1106	8	0.04888969276959898
1106	9	0.06446542730120797
1106	10-11	0.041527421174265555
1106	12-13	0.0739769117826583
1106	14-15	0.1260818089507083
1106	16-17	0.0886259811282315
1106	18-19	0.11516895422512619
1106	20-21	0.10694032633885087
1106	22-23	0.1389927288374082
1106	24-25	0.0753365615872994
1106	26-27	0.05359222367079042
1106	28-29	0.08380174889914116
1106	30-31	0.13060723498827542
1106	32-33	0.11963494802053276
1106	34-35	0.08426012315116083
1106	36-37	0.08960879331497296
1106	38-39	0.08860542885581424
1106	40-41	0.116600124182348
1106	42-43	0.11906870994716456
1106	44-45	0.19571166970677467
1106	46-47	0.19489386581383883
1106	48-49	0.11367587405074175
1106	50-51	0.09462178760465889
1106	52-53	0.0998431998037006
1106	54-55	0.12827126031108804
1106	56-57	0.0691717917757444
1106	58-59	0.054649982195300595
1106	60-61	0.1314703454060009
1106	62-63	0.10915039060975573
1106	64-65	0.08987319973557817
1106	66-67	0.1276996574940128
1106	68-69	0.07394093689271841
1106	70-71	0.10246611564814856
1106	72-73	0.14950348902282684
1106	74-75	0.12158641362486122
1106	76-77	0.07544539584056054
1106	78-79	0.08897749072502847
1106	80-81	0.10778334761444341
1106	82-83	0.062153886662372315
1106	84-85	0.10410851708075342
1106	86-87	0.11148451257576397
1106	88-89	0.0880428026260347
1106	90-91	0.08938927981070321
1106	92-93	0.11348386487925666
1106	94-95	0.0398982025133634
1106	96-97	0.06047096663662899
1106	98-99	0.1255770792986297
1106	100	0.181382929995479
1107	1	-0.08900671365942259
1107	2	-0.038164070101430525
1107	3	-0.045641837829634824
1107	4	-0.03912808075930485
1107	5	-0.11619705778631584
1107	6	-0.15879278332651836
1107	7	-0.1027663576591138
1107	8	-0.06935768636178352
1107	9	-0.09103612637404268
1107	10-11	-0.12050180434260938
1107	12-13	-0.09888249371520885
1107	14-15	-0.09745040075043931
1107	16-17	-0.06488243622030865
1107	18-19	-0.06491311702531277
1107	20-21	-0.04736830548710458
1107	22-23	-0.036171220752478916
1107	24-25	0.00545803613938034
1107	26-27	-0.03378182278019182
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2216	5	-0.04137162210734857
2216	6	-0.04496168911892795
2216	7	-0.05619959044883416
2216	8	-0.069840999843791
2216	9	-0.09160672701774075
2216	10-11	-0.11186927742528496
2216	12-13	-0.06915451358547386
2216	14-15	-0.15157287537414987
2216	16-17	-0.12990215284977324
2216	18-19	-0.13012109452509435
2216	20-21	-0.15932412604710322
2216	22-23	-0.1592397005648749
2216	24-25	-0.19367497029053737
2216	26-27	-0.2301070866188084
2216	28-29	-0.21122237594945403
2216	30-31	-0.1720757284640868
2216	32-33	-0.1923088540956428
2216	34-35	-0.17197720129301786
2216	36-37	-0.26220427414450853
2216	38-39	-0.23911041846692171
2216	40-41	-0.28317834764363425
2216	42-43	-0.2743850974744859
2216	44-45	-0.1381088654444298
2216	46-47	-0.12534192705281555
2216	48-49	-0.1388555833503844
2216	50-51	-0.10955306249093866
2216	52-53	-0.0345006243351591
2216	54-55	-0.028865840903165463
2216	56-57	-0.0702485611878032
2216	58-59	-0.06405878783782981
2216	60-61	-0.04467268191213947
2216	62-63	-0.016517997830334252
2216	64-65	-0.01296497358293891
2216	66-67	0.03424915347487456
2216	68-69	0.006784365624305622
2216	70-71	-0.012132070483566793
2216	72-73	0.00154654336881066
2216	74-75	-0.034090733837025766
2216	76-77	-0.015229849662304673
2216	78-79	-0.014798949007897022
2216	80-81	-0.0033950661517394565
2216	82-83	-0.06336895186130675
2216	84-85	-0.04997964372488184
2216	86-87	-0.04044632618816024
2216	88-89	0.017216401432804673
2216	90-91	-0.07237028253852174
2216	92-93	-0.06244599287423114
2216	94-95	-0.09117800598138359
2216	96-97	-0.13486643088280914
2216	98-99	-0.09739458151200608
2216	100	-0.11139062676798517
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	19.0
3	6.0
4	10.0
5	45.0
6	67.0
7	178.0
8	343.0
9	625.0
10	1171.0
11	1676.0
12	2632.0
13	3958.0
14	4595.0
15	5907.0
16	7971.0
17	12163.0
18	14787.0
19	8480.0
20	5964.0
21	5660.0
22	5960.0
23	6584.0
24	7682.0
25	9283.0
26	11846.0
27	15344.0
28	19660.0
29	25541.0
30	32725.0
31	41748.0
32	53739.0
33	71595.0
34	103016.0
35	169592.0
36	351006.0
37	790099.0
38	1145062.0
39	367837.0
40	722.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.12298037258594	18.431944832230943	13.201248373063196	24.24382642211992
2	16.508736101656453	19.667407911655697	38.25265864911883	25.571197337569018
3	19.250730191347344	24.708422659621007	29.076833616817606	26.964013532214036
4	13.336969932405243	16.188886529032587	36.43699819017174	34.037145348390425
5	14.106324816952698	37.28166282493317	34.08802008677798	14.523992271336148
6	32.577364501504405	35.99406406992417	17.10455496408036	14.324016464491066
7	29.275861755976244	31.63976436915499	21.14665811440864	17.937715760460126
8	27.2274692031282	34.18329482432236	19.235243414565815	19.353992557983617
9	26.7805305006243	15.845312352036567	19.655988137814106	37.71816900952503
10-11	25.873430336523022	25.389266558284735	27.148675311504768	21.588627793687476
12-13	26.51739722106751	22.823630426061435	27.320668212064387	23.338304140806667
14-15	23.585731379498654	24.131023100812484	25.087654808421036	27.19559071126783
16-17	23.305432672031387	27.56530576063036	25.586301144405134	23.54296042293312
18-19	23.348774716209185	26.5282786365092	27.5008996926296	22.62204695465201
20-21	24.212688235674968	25.468520538844004	27.416832007280433	22.9019592182006
22-23	23.804337319215964	25.336562701523512	27.37358486341821	23.485515115842315
24-25	23.64167192338354	25.612297843308994	27.348375863576475	23.39765436973099
26-27	23.344886645728305	25.717788359573856	27.512459696780006	23.424865297917833
28-29	23.385915171638697	25.874237168492893	27.49810630042585	23.24174135944256
30-31	23.549830008639166	25.91236717225988	27.101920818391847	23.43588200070911
32-33	23.454955532932985	25.650603970139823	27.39316888527896	23.501271611648228
34-35	23.648606321599935	25.55159280773881	27.442126144501927	23.35767472615933
36-37	23.867461748898467	25.47033118675253	27.03876252844623	23.623444535902774
38-39	23.65114891257903	25.954859955285432	27.063554844340292	23.330436287795248
40-41	23.608656211201506	25.817059875179027	27.137054155415658	23.437229758203813
42-43	23.534326497386967	25.882424143388437	26.912052629827	23.671196729397597
44-45	23.557877363971762	25.706997622713494	26.95481582081748	23.780309192497263
46-47	23.83598074516053	25.77816751020438	26.78911163852075	23.596740106114336
48-49	23.866929260367463	25.96647361450708	26.437868440313846	23.72872868481161
50-51	23.714113986545804	26.063840489696798	26.4136520138557	23.808393509901695
52-53	23.892860146838043	25.993997830045217	26.307116374565904	23.806025648550833
54-55	23.719595895784416	26.16699145918219	26.335106001566626	23.778306643466767
56-57	23.637284491137454	26.048962711969022	26.39848458329444	23.915268213599088
58-59	23.888438850351974	25.714369995212195	26.488617806852538	23.908573347583292
60-61	23.677999979124344	25.954444782455255	26.708192075994646	23.659363162425755
62-63	23.48002077900703	26.038534347837793	26.52374594547659	23.957698927678585
64-65	23.588052934220404	26.037680361818126	26.42296759058047	23.951299113381005
66-67	23.572104545328028	25.834315598901636	26.396566830565842	24.19701302520449
68-69	23.645874618412414	25.961252787215567	26.454858453318487	23.93801414105353
70-71	23.705414207851973	25.82541293715973	26.536183717894218	23.93298913709408
72-73	23.687702942184217	25.848985676715984	26.425018133850887	24.038293247248912
74-75	23.5174184835061	26.094626721713908	26.29415583904792	24.09379895573207
76-77	23.62890478631731	25.79277167047395	26.28797836435661	24.29034517885212
78-79	23.654386778067682	25.95225964633727	26.327868971493025	24.06548460410202
80-81	23.702749004436075	25.834424388196187	26.419033140082366	24.043793467285372
82-83	23.528430271669176	25.871796062582735	26.50522034612107	24.094553319627014
84-85	23.50222007616876	25.964407985739236	26.160411848635462	24.372960089456544
86-87	23.526655045913472	25.942976017107107	26.32336583725469	24.20700309972473
88-89	23.30924473375774	25.92825215759668	26.293045286688223	24.469457821957356
90-91	23.41781994174805	25.809329691098863	26.34711797457233	24.425732392580755
92-93	23.52684191466001	25.910893937821623	26.33394215406361	24.22832199345475
94-95	23.469063908912297	25.678773895727403	26.391190748912805	24.46097144644749
96-97	23.39902483830505	25.73728601778115	26.42247385863544	24.44121528527836
98-99	23.01073912246339	26.01617161296803	26.375685339113147	24.597403925455435
100	23.286251345567027	25.474162995288168	26.274181632034388	24.965404027110417
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	55.0
1	43.5
2	129.5
3	243.0
4	182.5
5	138.5
6	310.0
7	723.5
8	1298.5
9	1508.5
10	1190.5
11	933.0
12	961.5
13	1121.0
14	1299.0
15	1460.5
16	1653.5
17	1868.0
18	2019.5
19	2197.0
20	2457.5
21	2824.0
22	3375.5
23	4204.5
24	5461.5
25	7131.0
26	9201.5
27	11957.0
28	15780.5
29	20457.5
30	25095.0
31	29299.0
32	34147.0
33	41233.5
34	48573.5
35	55152.5
36	62990.5
37	72135.0
38	79666.5
39	85024.0
40	92231.0
41	100732.5
42	108969.5
43	116778.0
44	119475.5
45	121239.0
46	123687.5
47	123804.5
48	128813.5
49	130580.5
50	125598.0
51	122619.5
52	118721.0
53	116868.0
54	115515.5
55	111678.0
56	104516.5
57	96364.5
58	88393.5
59	78467.5
60	70157.5
61	62627.0
62	56079.0
63	48057.0
64	38870.5
65	32307.0
66	26623.5
67	21288.5
68	17710.0
69	14720.0
70	11015.5
71	7549.0
72	5482.0
73	4173.5
74	3012.0
75	1864.5
76	1146.0
77	883.0
78	606.5
79	294.5
80	146.5
81	65.0
82	39.0
83	20.5
84	10.0
85	7.5
86	4.5
87	2.5
88	2.0
89	1.5
90	1.0
91	0.5
92	1.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09318373108869457
2	0.0014522139909926427
3	0.0
4	3.630534977481607E-4
5	0.03733400135176919
6	9.076337443704017E-5
7	0.0038725706426470476
8	3.3279903960248063E-4
9	1.512722907284003E-4
10-11	3.4792626867532063E-4
12-13	0.0
14-15	9.076337443704017E-5
16-17	0.0
18-19	7.563614536420015E-5
20-21	0.0
22-23	2.2690843609260043E-4
24-25	9.076337443704017E-5
26-27	0.0012404327839728824
28-29	0.007851031888803974
30-31	0.03508004421991603
32-33	0.025111200260914446
34-35	0.027622320287005894
36-37	0.024748146763166286
38-39	0.023658986269921804
40-41	0.029210679339654093
42-43	0.02142015636714148
44-45	0.02087557612051924
46-47	0.014113704724959746
48-49	0.009651172148471937
50-51	0.007034161518870613
52-53	0.0048407133033088095
54-55	0.0033582448541704863
56-57	0.002072430382979084
58-59	7.714886827148414E-4
60-61	3.781807268210007E-4
62-63	0.0011799238676815222
64-65	0.0030708275017865255
66-67	4.235624140395208E-4
68-69	8.471248280790416E-4
70-71	3.933079558938408E-4
72-73	4.6894410125804085E-4
74-75	4.8407133033088096E-4
76-77	3.4792626867532063E-4
78-79	6.050891629136012E-5
80-81	3.176718105296406E-4
82-83	2.8741735238396054E-4
84-85	1.8152674887408034E-4
86-87	1.3614506165556025E-4
88-89	0.0
90-91	3.025445814568006E-5
92-93	3.025445814568006E-5
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3305298.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.24730868991216
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.85352197904385	24.422253660705813
2	16.206244069738432	12.396904392786883
3	6.882523531431755	7.897140062167642
4	3.6561215058710768	5.593472313715102
5	2.2618573679681107	4.325497848261429
6	1.4953040425958137	3.4314813181461385
7	1.0447212042834952	2.797044207658934
8	0.7546846563045743	2.309172561057705
9	0.5472842890643129	1.8838936030483706
>10	2.985315132949342	20.612841990078568
>50	0.20297860346571808	5.389075289183822
>100	0.10493237485720976	7.145596227637791
>500	0.003324073367217757	0.8457325562802787
>1k	0.001108024455286416	0.7808019452959775
>5k	7.91446039490297E-5	0.1690920239754722
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5586	0.1690014032017688	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4506	0.13632658840443435	No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	4358	0.1318489285988737	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	6.050891629136011E-5	0.0
22-23	0.0	0.0	0.0	1.9665397794692036E-4	0.0
24-25	0.0	0.0	0.0	3.933079558938407E-4	0.0
26-27	0.0	0.0	0.0	5.899619338407612E-4	0.0
28-29	0.0	0.0	0.0	0.001603486281721043	0.0
30-31	0.0	0.0	0.0	0.006050891629136012	0.0
32-33	0.0	0.0	0.0	0.01529362859264127	0.0
34-35	0.0	0.0	0.0	0.02830304559528369	0.0
36-37	0.0	0.0	0.0	0.05164436005467586	0.0
38-39	0.0	0.0	0.0	0.08902374309366357	0.0
40-41	0.0	0.0	0.0	0.13269605342695273	0.0
42-43	0.0	0.0	0.0	0.17704908906851968	0.0
44-45	0.0	0.0	0.0	0.23103816962948576	0.0
46-47	0.0	0.0	0.0	0.29849048406527945	0.0
48-49	0.0	0.0	0.0	0.3732643773723277	0.0
50-51	0.0	0.0	0.0	0.4517141873440761	0.0
52-53	0.0	0.0	0.0	0.5319490103464196	0.0
54-55	0.0	0.0	0.0	0.6199894835503486	0.0
56-57	0.0	0.0	0.0	0.7245640181308917	0.0
58-59	0.0	0.0	0.0	0.8406655012649389	0.0
60-61	0.0	0.0	0.0	0.9636044919399098	0.0
62-63	0.0	0.0	0.0	1.0893420199933561	0.0
64-65	0.0	0.0	0.0	1.2224918902924942	0.0
66-67	0.0	0.0	0.0	1.3737793082499672	0.0
68-69	0.0	0.0	0.0	1.5460481929314693	0.0
70-71	0.0	0.0	0.0	1.73103907726323	0.0
72-73	0.0	0.0	0.0	1.9258324060341911	0.0
74-75	0.0	0.0	0.0	2.122909946395151	0.0
76-77	0.0	0.0	0.0	2.338230925017956	0.0
78-79	0.0	0.0	0.0	2.581174223927767	0.0
80-81	0.0	0.0	0.0	2.846036877764123	0.0
82-83	0.0	0.0	0.0	3.121382701347957	0.0
84-85	0.0	0.0	0.0	3.3981958661518568	0.0
86-87	0.0	0.0	0.0	3.690877494253166	0.0
88	0.0	0.0	0.0	3.9350460987178764	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	13640	0.0	27.248428	1
GTACATG	13760	0.0	25.506397	1
TACATGG	13720	0.0	24.361036	2
GGTATCA	10170	0.0	24.332878	1
TCAACGC	15370	0.0	23.917355	4
ACATGGG	13555	0.0	23.721212	3
ATCAACG	15500	0.0	23.716759	3
CAACGCA	15555	0.0	23.572458	5
AACGCAG	15895	0.0	23.119686	6
TATCAAC	16170	0.0	22.85035	2
CCGTTCG	165	2.669667E-6	22.784618	9
ACGCAGA	17955	0.0	20.257751	7
CGCAGAG	18295	0.0	19.85559	8
GAGTACT	7730	0.0	19.788242	12-13
GTATATA	1400	0.0	19.490757	1
AGAGTAC	14085	0.0	19.267742	10-11
TATACTG	905	0.0	19.219116	5
TATAACG	175	1.0769266E-4	18.80357	2
GAGTACA	9630	0.0	18.711182	1
AGTACTT	7890	0.0	18.702013	12-13
>>END_MODULE