FastQCFastQC Report
Wed 25 May 2016
SRR1033126_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033126_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2439805
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA28400.033.499511
TCAACGC26950.032.424394
ATCAACG27700.031.3839473
CTATGCG953.771483E-529.6639869
CAACGCA29700.029.4215285
AACGCAG30550.028.2872476
GTACATG62000.027.8791681
GGTATCA18200.027.4308911
TATCAAC34150.025.8824562
TACATGG63950.025.2904322
ACATGGG64700.024.5486743
ACGCAGA36250.023.580687
GCGTATA1201.813005E-423.5491621
CGCAGAG36700.022.9080878
CATGGGG41250.019.7033394
GCAGAGT47200.017.5134859
GAGTACA44500.016.828391
ACACCGT4205.638867E-1116.773596
GTATTAG12000.016.4844131
TACACCG2859.640462E-716.4840475
Produced by FastQC (version 0.11.5)