##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033126_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2439805
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.62738497543861	33.0	31.0	34.0	30.0	34.0
2	31.79342201528401	34.0	31.0	34.0	30.0	34.0
3	31.91358612676013	34.0	31.0	34.0	30.0	34.0
4	35.25868747707296	37.0	35.0	37.0	33.0	37.0
5	35.32865372437551	37.0	35.0	37.0	33.0	37.0
6	35.44171931773236	37.0	37.0	37.0	35.0	37.0
7	35.375804623730176	37.0	37.0	37.0	35.0	37.0
8	35.377142025694674	37.0	37.0	37.0	33.0	37.0
9	37.04266857392292	39.0	38.0	39.0	35.0	39.0
10-11	37.03186607126389	39.0	38.0	39.0	34.5	39.0
12-13	36.97644770791108	39.0	38.0	39.0	34.0	39.0
14-15	38.44523086886042	41.0	39.0	41.0	34.5	41.0
16-17	38.455553415129486	41.0	39.0	41.0	34.0	41.0
18-19	38.448959035660636	41.0	39.0	41.0	34.0	41.0
20-21	38.47513243886294	41.0	39.0	41.0	34.0	41.0
22-23	38.44029645811858	41.0	39.0	41.0	34.0	41.0
24-25	38.43386623111273	41.0	39.0	41.0	34.0	41.0
26-27	38.39172864224805	41.0	39.0	41.0	34.0	41.0
28-29	38.36227116511361	40.5	39.0	41.0	34.0	41.0
30-31	38.36045893011942	41.0	39.0	41.0	34.0	41.0
32-33	38.42663921911792	41.0	39.0	41.0	34.0	41.0
34-35	38.39967067040194	41.0	39.0	41.0	34.0	41.0
36-37	38.322571271064696	41.0	39.0	41.0	34.0	41.0
38-39	38.2490918331588	41.0	39.0	41.0	33.0	41.0
40-41	38.19088123845963	41.0	39.0	41.0	33.0	41.0
42-43	38.08394318398396	40.5	38.0	41.0	33.0	41.0
44-45	37.9719303386951	40.0	38.0	41.0	33.0	41.0
46-47	37.85591266515152	40.0	38.0	41.0	33.0	41.0
48-49	37.74408118681616	40.0	38.0	41.0	33.0	41.0
50-51	37.63103383262187	40.0	38.0	41.0	32.0	41.0
52-53	37.491715936314584	40.0	37.5	41.0	32.0	41.0
54-55	37.35064790013956	40.0	37.0	41.0	32.0	41.0
56-57	37.20295392459643	40.0	37.0	41.0	31.0	41.0
58-59	37.01820924213206	40.0	36.0	41.0	31.0	41.0
60-61	36.80331276474964	40.0	36.0	41.0	31.0	41.0
62-63	36.54822946915839	39.0	35.0	41.0	31.0	41.0
64-65	36.28329620604926	39.0	35.0	41.0	30.5	41.0
66-67	36.00090376075137	38.5	35.0	41.0	30.0	41.0
68-69	35.69704136191212	38.0	35.0	40.0	30.0	41.0
70-71	35.346755171007516	37.0	35.0	40.0	29.5	41.0
72-73	34.988579825027	37.0	35.0	39.5	29.0	41.0
74-75	34.61742536801097	36.0	34.0	39.0	29.0	41.0
76-77	34.28046278288634	36.0	34.0	39.0	29.0	40.5
78-79	33.90514651785696	35.0	34.0	37.5	29.0	39.5
80-81	33.53486467156186	35.0	34.0	37.0	28.0	39.0
82-83	33.30308918130753	35.0	34.0	37.0	29.0	39.0
84-85	33.023405149182004	35.0	34.0	36.0	28.0	38.0
86-87	32.75800074186257	35.0	34.0	36.0	28.0	37.0
88-89	32.38430755736627	35.0	34.0	36.0	27.0	37.0
90-91	32.18795252079572	35.0	34.0	35.0	27.0	36.5
92-93	32.006447851365174	35.0	34.0	35.0	26.5	36.0
94-95	31.83186853047682	35.0	34.0	35.0	26.0	36.0
96-97	31.65340467783286	35.0	33.0	35.0	25.5	36.0
98-99	31.46350671467597	35.0	33.0	35.0	25.0	35.5
100	31.302210627488673	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.1969558957902926
1101	2	0.1631310835010602
1101	3	0.2774730797749214
1101	4	0.15352058380222644
1101	5	0.15744846042713334
1101	6	0.1595564185357503
1101	7	0.24415221642505003
1101	8	0.18953251297558893
1101	9	0.2371381824134673
1101	10-11	0.216663282337052
1101	12-13	0.1727978843962319
1101	14-15	0.2635025896668779
1101	16-17	0.1927255083000574
1101	18-19	0.1639878699022148
1101	20-21	0.16040522031998705
1101	22-23	0.17042441404922926
1101	24-25	0.14957704801169314
1101	26-27	0.17397081022776462
1101	28-29	0.2170914548644447
1101	30-31	0.24852426999518684
1101	32-33	0.3270662914759299
1101	34-35	0.31155722622588655
1101	36-37	0.26795081781040864
1101	38-39	0.264983445058391
1101	40-41	0.24303700944508222
1101	42-43	0.2625032243574452
1101	44-45	0.1821680875684777
1101	46-47	0.1950717061395153
1101	48-49	0.12951341921051096
1101	50-51	0.16766648022183261
1101	52-53	0.17323568592014738
1101	54-55	0.15106695196748632
1101	56-57	0.22916018987295672
1101	58-59	0.26197681948357143
1101	60-61	0.22683085119994928
1101	62-63	0.22236622878033785
1101	64-65	0.21203304873925077
1101	66-67	0.23121489618068836
1101	68-69	0.30235867904274727
1101	70-71	0.27437077516505326
1101	72-73	0.24090601537175615
1101	74-75	0.23558612285962965
1101	76-77	0.2381399099651773
1101	78-79	0.2643915079667991
1101	80-81	0.2603265589371304
1101	82-83	0.2304486309080005
1101	84-85	0.1860902506977169
1101	86-87	0.22238593939595575
1101	88-89	0.38991015096451775
1101	90-91	0.41750579452737924
1101	92-93	0.42982564471989093
1101	94-95	0.45325388833732916
1101	96-97	0.49691620257296165
1101	98-99	0.562961603755646
1101	100	0.5984038684113457
1102	1	0.22605363826643554
1102	2	0.23300100165209514
1102	3	0.2504183198891283
1102	4	0.20258795166096633
1102	5	0.1025910715896714
1102	6	0.07889936214217386
1102	7	0.12439972547304023
1102	8	0.16417679543360464
1102	9	0.21359067950263722
1102	10-11	0.2370733137402965
1102	12-13	0.22453495984093763
1102	14-15	0.26796391562178457
1102	16-17	0.2376871596972947
1102	18-19	0.2314841768016791
1102	20-21	0.21325467752712512
1102	22-23	0.24950746191820627
1102	24-25	0.2412992732592798
1102	26-27	0.23188532564715558
1102	28-29	0.21206576955550815
1102	30-31	0.17566819157093505
1102	32-33	0.2523953426278567
1102	34-35	0.24678339783989145
1102	36-37	0.2364600153379044
1102	38-39	0.18150588847767324
1102	40-41	0.2241566434283513
1102	42-43	0.23125738963027942
1102	44-45	0.16447063742857182
1102	46-47	0.1779893117483553
1102	48-49	0.22809634044655525
1102	50-51	0.21021951262689953
1102	52-53	0.07676096539861987
1102	54-55	0.1441564671923956
1102	56-57	0.06374321310686071
1102	58-59	0.040124728484187244
1102	60-61	0.045167430312687884
1102	62-63	0.12428050389969059
1102	64-65	0.16830868326535153
1102	66-67	0.06418877349108953
1102	68-69	0.02752202688916583
1102	70-71	0.11352719234321995
1102	72-73	0.19951887038698857
1102	74-75	0.14566783351936152
1102	76-77	0.053374760041286606
1102	78-79	0.12751184729120268
1102	80-81	0.0672708845281349
1102	82-83	0.011797803299081977
1102	84-85	-0.011819138896939307
1102	86-87	0.061517375741942715
1102	88-89	0.1514404905741813
1102	90-91	0.17595208184844324
1102	92-93	0.20026592972612178
1102	94-95	0.16140416576286754
1102	96-97	0.14906226351063268
1102	98-99	0.1858896425355745
1102	100	0.19574535465848797
1103	1	0.26364684861186305
1103	2	0.24016939548186045
1103	3	0.2522115928879458
1103	4	0.35878567932609684
1103	5	0.31385398378448315
1103	6	0.23861693863376132
1103	7	0.2704379218942776
1103	8	0.2737763556853068
1103	9	0.32272184943142435
1103	10-11	0.25435845194941464
1103	12-13	0.3240100992464221
1103	14-15	0.27573724020982127
1103	16-17	0.29325643221824294
1103	18-19	0.2852163407616146
1103	20-21	0.3038735232449099
1103	22-23	0.28795141775343325
1103	24-25	0.34859551591818416
1103	26-27	0.3088915723615884
1103	28-29	0.3192922947939749
1103	30-31	0.37977793702600593
1103	32-33	0.34444419247499525
1103	34-35	0.32626092156436926
1103	36-37	0.32187383898521915
1103	38-39	0.28884017788768546
1103	40-41	0.32458123174740194
1103	42-43	0.3039165328560358
1103	44-45	0.18901909757965285
1103	46-47	0.2409015233376266
1103	48-49	0.3287566340516932
1103	50-51	0.27807272616372103
1103	52-53	0.2386474583908864
1103	54-55	0.3247738749359428
1103	56-57	0.28808854471596135
1103	58-59	0.2247226150144357
1103	60-61	0.22646338739024685
1103	62-63	0.24955087913042462
1103	64-65	0.21069129582458856
1103	66-67	0.22571073917669793
1103	68-69	0.26228369482517877
1103	70-71	0.31468044935485295
1103	72-73	0.3212764020916339
1103	74-75	0.26546199346471155
1103	76-77	0.27843291242393775
1103	78-79	0.25868244724527045
1103	80-81	0.3182859819152455
1103	82-83	0.34481224864791926
1103	84-85	0.36514018488938405
1103	86-87	0.3240894988748195
1103	88-89	0.29742528731056694
1103	90-91	0.3434866087507302
1103	92-93	0.4002529510438819
1103	94-95	0.3837781135625562
1103	96-97	0.3695844209820649
1103	98-99	0.42964359927184503
1103	100	0.4891756581281008
1104	1	0.13960410855553818
1104	2	0.1358588494993569
1104	3	0.11162044942615523
1104	4	0.17179153226374666
1104	5	0.034388040038798806
1104	6	0.11339550897433526
1104	7	0.13092200456134861
1104	8	0.1517084149406287
1104	9	0.16972829544083368
1104	10-11	0.18413515071109998
1104	12-13	0.1958144795512453
1104	14-15	0.1088193417579646
1104	16-17	0.18766640684255265
1104	18-19	0.20535388155555978
1104	20-21	0.14541678183392293
1104	22-23	0.16325982316821097
1104	24-25	0.14940195577000992
1104	26-27	0.2291019102828571
1104	28-29	0.20587177775207266
1104	30-31	0.17516562932870983
1104	32-33	0.23605255122183166
1104	34-35	0.22618504564923114
1104	36-37	0.21989498863698742
1104	38-39	0.26840160456553974
1104	40-41	0.22166719960485892
1104	42-43	0.22750814947890063
1104	44-45	0.22711942069033597
1104	46-47	0.1805680582105751
1104	48-49	0.04510706329953251
1104	50-51	0.0514743086606515
1104	52-53	0.05262572214901695
1104	54-55	0.08228575257520276
1104	56-57	0.10757062779137527
1104	58-59	-0.001563508078739062
1104	60-61	0.10417049258432343
1104	62-63	0.0687120390880338
1104	64-65	-0.05768663672078844
1104	66-67	-0.032420177362226354
1104	68-69	0.04864835131348144
1104	70-71	0.013043830576840776
1104	72-73	0.07741765201465967
1104	74-75	0.12603702190298804
1104	76-77	-0.0356726349035128
1104	78-79	0.03321945427462225
1104	80-81	0.12047564820705503
1104	82-83	0.16173922142171193
1104	84-85	0.12611667105265667
1104	86-87	0.09616655445179845
1104	88-89	0.2798768855255176
1104	90-91	0.2767003284773253
1104	92-93	0.16645936594132849
1104	94-95	0.18384118820209494
1104	96-97	0.22444137077837922
1104	98-99	0.22026184416834838
1104	100	0.264637152215208
1105	1	0.03899675122853452
1105	2	0.09186548284896645
1105	3	0.05062278804730269
1105	4	0.07990898888886022
1105	5	0.015477395891728918
1105	6	0.011896552335237232
1105	7	-0.03049090201508875
1105	8	-0.08012497696189058
1105	9	-0.07373755975746832
1105	10-11	-0.007657634082889331
1105	12-13	-0.05346923997592512
1105	14-15	0.009722797443892262
1105	16-17	-0.06337827574871113
1105	18-19	-0.01926601476142764
1105	20-21	-0.03837765430026252
1105	22-23	0.05369071877925791
1105	24-25	-0.005369243426393666
1105	26-27	0.03073540760991733
1105	28-29	0.09995159874803505
1105	30-31	0.06324834094748866
1105	32-33	0.09839530849347966
1105	34-35	0.09874041685868917
1105	36-37	0.04276962672603446
1105	38-39	0.04708131764907364
1105	40-41	0.03860645920735806
1105	42-43	0.02218592044951606
1105	44-45	0.045319148887820404
1105	46-47	0.10745294953621709
1105	48-49	0.1198628219986162
1105	50-51	0.08833437546062584
1105	52-53	0.05807444508149473
1105	54-55	0.014677637995198722
1105	56-57	-0.016370539715467203
1105	58-59	0.0143667158006906
1105	60-61	-0.08050147070679259
1105	62-63	0.03548876918565469
1105	64-65	0.11466227809057727
1105	66-67	0.02499996898818324
1105	68-69	0.05458209711904516
1105	70-71	0.009851861501374515
1105	72-73	0.027805025015403828
1105	74-75	0.008296193682802766
1105	76-77	0.07698592951897609
1105	78-79	0.06055041736981792
1105	80-81	0.026170594981650197
1105	82-83	-0.03178342775090215
1105	84-85	-0.010992185230982443
1105	86-87	-0.0404222540642607
1105	88-89	0.019895645807515905
1105	90-91	0.026543202369047947
1105	92-93	-0.03183533764155655
1105	94-95	-0.015506477997050183
1105	96-97	0.009979315839441227
1105	98-99	0.04387488167025566
1105	100	0.06826986741033636
1106	1	0.13660442335600465
1106	2	0.1306737226105561
1106	3	0.16712400909295155
1106	4	0.16697447976471835
1106	5	0.14149466053473247
1106	6	0.15132048960199995
1106	7	0.17861488038467144
1106	8	0.17001042966360558
1106	9	0.17366451202354938
1106	10-11	0.15267931963786907
1106	12-13	0.1517115397992157
1106	14-15	0.23162633274983335
1106	16-17	0.20355717108122207
1106	18-19	0.17559645952920988
1106	20-21	0.14522366385639174
1106	22-23	0.2015562078059503
1106	24-25	0.20843218872235525
1106	26-27	0.1846127345757651
1106	28-29	0.2830675109949965
1106	30-31	0.20368715683742522
1106	32-33	0.169574865731704
1106	34-35	0.148781665176557
1106	36-37	0.1640206303456324
1106	38-39	0.1957520138061497
1106	40-41	0.1274492366060187
1106	42-43	0.12925952975786004
1106	44-45	0.12379645962872843
1106	46-47	0.12718909898934072
1106	48-49	0.08817782075788472
1106	50-51	0.09454809304654788
1106	52-53	0.08466514485338905
1106	54-55	0.09463349811956334
1106	56-57	0.04732992651280199
1106	58-59	0.09389943022036817
1106	60-61	0.05050004024027288
1106	62-63	0.13155044958784146
1106	64-65	0.13527696750237794
1106	66-67	0.19890640390472214
1106	68-69	0.12843634530805303
1106	70-71	0.10544200473414378
1106	72-73	0.14875658314600315
1106	74-75	0.11260563629663523
1106	76-77	0.11382913282217544
1106	78-79	0.06416706421263285
1106	80-81	0.10295942387539725
1106	82-83	0.007573261038139378
1106	84-85	0.007038854799276351
1106	86-87	0.023184001662180265
1106	88-89	0.047331965920712094
1106	90-91	0.05493974633862564
1106	92-93	0.09343413347328777
1106	94-95	0.03498203719473736
1106	96-97	0.05879851190584873
1106	98-99	0.1278766857994995
1106	100	0.17131818669796672
1107	1	0.15919891959809007
1107	2	0.1548073611020584
1107	3	0.1870156388925608
1107	4	0.2193131404453723
1107	5	0.16964807171383
1107	6	0.14451458375197035
1107	7	0.12186423205276498
1107	8	0.12820766497880243
1107	9	0.17628439887269565
1107	10-11	0.15695520927098983
1107	12-13	0.15393210530083934
1107	14-15	0.1994466526433385
1107	16-17	0.1509701458737993
1107	18-19	0.21482316006532898
1107	20-21	0.196397308274193
1107	22-23	0.1330474013453724
1107	24-25	0.11972079433212457
1107	26-27	0.18561344263000734
1107	28-29	0.15509180248134413
1107	30-31	0.14020023086096955
1107	32-33	0.15547985582009716
1107	34-35	0.1443899199520402
1107	36-37	0.1102287141659346
1107	38-39	0.15784035319211398
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2216	28-29	-0.2673505087428296
2216	30-31	-0.29796791997804917
2216	32-33	-0.23627345416502976
2216	34-35	-0.2951344430902765
2216	36-37	-0.2746001422769879
2216	38-39	-0.2852251285372631
2216	40-41	-0.2512784166682991
2216	42-43	-0.21184609944747024
2216	44-45	-0.16906755944338414
2216	46-47	-0.12678018150702286
2216	48-49	-0.17079987660063267
2216	50-51	-0.17826355994245802
2216	52-53	-0.13652484119816677
2216	54-55	-0.18862391174064186
2216	56-57	-0.2123985643612727
2216	58-59	-0.1732602509171457
2216	60-61	-0.16717404528505142
2216	62-63	-0.10838330771329652
2216	64-65	-0.09930563687315441
2216	66-67	-0.09521615090827851
2216	68-69	-0.14047125569924646
2216	70-71	-0.16439000227573786
2216	72-73	-0.03001409556241441
2216	74-75	0.0037857442682494025
2216	76-77	-0.13068876189485223
2216	78-79	-0.1152534849179716
2216	80-81	-0.09584334342343936
2216	82-83	-0.05293747049206132
2216	84-85	-0.06877701863251673
2216	86-87	-0.131128120154834
2216	88-89	-0.11818803691256363
2216	90-91	-0.15168220788910247
2216	92-93	-0.11769809237253526
2216	94-95	-0.11196505161362325
2216	96-97	-0.11691351599696986
2216	98-99	-0.11482833915512813
2216	100	-0.15177861427525485
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	24981.0
3	4480.0
4	2797.0
5	2272.0
6	1959.0
7	2211.0
8	2599.0
9	2827.0
10	3094.0
11	3248.0
12	3794.0
13	4342.0
14	4870.0
15	5092.0
16	5259.0
17	5082.0
18	4976.0
19	4948.0
20	4995.0
21	5171.0
22	5941.0
23	7955.0
24	12385.0
25	17079.0
26	17733.0
27	15425.0
28	16224.0
29	20295.0
30	25015.0
31	30407.0
32	39799.0
33	54369.0
34	75957.0
35	114424.0
36	214224.0
37	467530.0
38	813545.0
39	391389.0
40	1112.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.69220931638349	20.083625229356667	13.510968409434652	23.713197044825197
2	15.093255684550726	20.13273936829389	41.16044770607266	23.613557241082727
3	18.113746913732147	25.498806506385808	29.981748980813872	26.405697599068173
4	11.931427259345515	16.739883634634072	39.64594258816583	31.682746517854582
5	12.60228508446054	38.231864233310866	36.10571221910043	13.060138463128165
6	30.619988850448443	38.58945875485743	18.005828919969176	12.784723474724952
7	26.94866094119422	32.62781477801497	22.885165910812486	17.538358369978326
8	24.411922288435296	37.42057061667249	20.527286896001566	17.640220198890646
9	24.37010478917952	17.43283317769231	21.547577370259745	36.649484662868424
10-11	23.377877460127973	27.387750768251927	29.286468599239786	19.947903172380318
12-13	24.019586743488677	24.631703222712094	29.31397205466087	22.034737979138356
14-15	21.339408441500677	26.783269990034242	26.237920464975755	25.63940110348933
16-17	21.09986830440642	30.16993343479531	26.787170696961777	21.94302756383649
18-19	21.006699331132744	29.140471142288842	28.7254422083278	21.127387318250616
20-21	22.122436613692972	28.501541162895904	28.233054741261533	21.142967482149587
22-23	21.75876714906295	28.30803154357657	28.265090990928577	21.668110316431903
24-25	21.65547856303588	28.33507160960283	28.192791237119742	21.81665859024155
26-27	21.40337420797859	28.29156226632909	28.312592648467117	21.992470877225205
28-29	21.44015391070482	28.36529523499525	28.36662756450371	21.82792328979622
30-31	21.70609371893339	28.80087237605639	27.95661896884986	21.536414936160355
32-33	21.339725041222497	28.450103763443945	28.4981317660448	21.712039429288758
34-35	21.486333703911402	28.243521442696494	28.540742271151647	21.729402582240457
36-37	21.795968041764926	28.593221151855726	27.995736114601037	21.61507469177831
38-39	21.562678702092466	28.76097832926482	28.125408644928296	21.550934323714422
40-41	21.35516101760049	28.38137985806881	28.50060205467169	21.762857069659006
42-43	21.374928036048686	28.278241072934605	28.455688959146606	21.891141931870095
44-45	21.4318887415959	28.51990627902649	28.364428824282196	21.683776155095423
46-47	21.544376776039442	28.613672160412033	28.2727135781898	21.569237485358723
48-49	21.43520575548061	28.479681041705263	28.32943666659562	21.75567653621851
50-51	21.44169986153661	28.37136636069181	28.306684527269255	21.880249250502327
52-53	21.528738056253584	28.33678367495507	28.324773575814366	21.80970469297698
54-55	21.62525850851933	28.743585576438903	28.131075885251423	21.500080029790347
56-57	21.49347459466372	28.443844378904497	28.171273624762705	21.891407401669074
58-59	21.366041064396907	28.098571603649418	28.53359942846111	22.00178790349256
60-61	21.693684905173154	28.51480298887983	28.13285185534346	21.658660250603557
62-63	21.445268213665916	28.492081513804372	28.202275330015485	21.860374942514223
64-65	21.465073937782254	28.234813837788565	28.344906904548573	21.955205319880612
66-67	21.374832232439303	28.321541528990867	28.218580560911843	22.085045677657984
68-69	21.761726521748752	28.443849842154407	28.170499474121275	21.623924161975566
70-71	21.69613245288811	28.252335638418764	28.23751854954021	21.814013359152913
72-73	21.487103323630862	28.347316627704444	28.334528466262928	21.831051582401766
74-75	21.549280418665504	28.413830586059568	28.216597424656275	21.820291570618654
76-77	21.599977374239508	28.38848430768247	28.06780924024582	21.943729077832206
78-79	21.68889936365779	28.529649858078265	28.071360501695885	21.710090276568057
80-81	21.577782327488595	28.31500230252257	28.255610131591162	21.851605238397674
82-83	21.48846184665787	28.37714957679126	28.360979910470462	21.773408666080403
84-85	21.488190010761414	28.283803715607696	28.217771967494016	22.010234306136876
86-87	21.676289916470985	28.410419751851723	28.09397352434736	21.819316807329926
88-89	21.488534651285246	28.320468980202268	28.16349074727329	22.02750562123919
90-91	21.471445806012227	28.271795422693703	28.23944412679646	22.01731464449761
92-93	21.67463013874874	28.41231720336771	28.071228780168173	21.841823877715377
94-95	21.40384303375658	28.21034659128509	28.23881671486934	22.14699366008899
96-97	21.48220269940431	28.13528686118386	28.28482772496685	22.09768271444498
98-99	21.50422370527529	28.26287365330613	28.163367453082422	22.06953518833615
100	21.587228421306083	28.218828026822262	28.044179879346004	22.14976367252565
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	350.0
1	318.5
2	276.5
3	238.5
4	201.0
5	170.5
6	150.5
7	153.5
8	152.0
9	148.5
10	162.5
11	172.0
12	176.5
13	186.5
14	206.5
15	267.5
16	399.0
17	546.0
18	773.0
19	1162.0
20	1746.0
21	2575.5
22	3939.5
23	6149.5
24	8957.5
25	12247.0
26	16483.0
27	22205.0
28	29321.0
29	37024.5
30	44291.0
31	51858.0
32	59580.5
33	66660.5
34	73322.5
35	78395.0
36	82072.0
37	85766.5
38	89272.0
39	90147.0
40	89749.0
41	90257.5
42	89026.0
43	87208.0
44	85785.5
45	83671.0
46	81761.0
47	78975.5
48	76194.5
49	74024.0
50	71469.0
51	68702.0
52	64479.5
53	60559.5
54	58003.5
55	54683.0
56	50211.0
57	45958.0
58	41964.5
59	37937.0
60	33965.0
61	29251.5
62	24871.0
63	20968.5
64	17120.0
65	13727.0
66	10763.0
67	7964.0
68	5836.0
69	4474.0
70	3222.5
71	2339.0
72	1798.0
73	1372.5
74	1031.5
75	766.0
76	527.5
77	364.5
78	250.5
79	164.5
80	108.0
81	58.5
82	41.5
83	28.0
84	22.0
85	21.5
86	15.0
87	7.5
88	6.5
89	8.5
90	7.0
91	6.0
92	5.0
93	3.5
94	3.5
95	3.0
96	2.5
97	4.0
98	3.0
99	1.0
100	2.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3280180178333924
2	0.1167716272407016
3	0.06496420820516394
4	0.038322734808724467
5	0.04233944925926457
6	0.01004178612635026
7	0.0074186256688546824
8	0.015329093923489788
9	0.017665346205946786
10-11	0.020534427956332577
12-13	0.016394752859347366
14-15	0.018526070731062525
16-17	0.019161367404362233
18-19	0.018731005141804365
20-21	0.02358795067638602
22-23	0.01582093650927021
24-25	0.024879037464059624
26-27	0.019550742784771733
28-29	0.0192023542865106
30-31	0.02008357225270052
32-33	0.024715089935466155
34-35	0.01528810704134142
36-37	0.01008277300849863
38-39	0.013935539930445261
40-41	0.00993931892097934
42-43	0.008832673102973393
44-45	0.009140074719086157
46-47	0.008709712456528287
48-49	0.004631517682765631
50-51	0.007008756847370999
52-53	0.007848987931412551
54-55	0.0036273390701306047
56-57	0.0029305620736083415
58-59	0.0031150030432759994
60-61	0.003893753804094999
62-63	0.0029100686325341575
64-65	0.002090330989566789
66-67	0.0018649031377507628
68-69	0.003237963689721105
70-71	0.0022337850770860787
72-73	0.0019878637841958683
74-75	0.0021723047538635258
76-77	0.0044265832720237885
78-79	0.0032789505718694733
80-81	0.0036683259522789736
82-83	0.0021108244306409733
84-85	0.002848588309311605
86-87	0.0022132916360118942
88-89	0.5995561120663332
90-91	0.6092085228122739
92-93	0.6055401968599949
94-95	0.5924858748957396
96-97	0.5894323521756861
98-99	0.5872600474218227
100	0.5884486670041253
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2439805.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.6592305503529
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.03192176057956	54.26270936997085
2	13.272441215016087	18.225512026955908
3	3.851786136733728	7.933820171779625
4	1.5024038453408304	4.126155679879713
5	0.7507730040572639	2.577374838827436
6	0.4266242968242325	1.7575017572422302
7	0.2666505557483541	1.2815615388454182
8	0.18319732953333176	1.00625501477104
9	0.127055334444665	0.7851169350259602
>10	0.5599702609439126	6.518393508234451
>50	0.022374553677642732	1.0284509691845725
>100	0.004741936702242128	0.4747004605021284
>500	5.977039804470274E-5	0.022447728780723715
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	4.0986882148368415E-5	0.0
10-11	0.0	0.0	0.0	8.197376429673683E-5	0.0
12-13	0.0	0.0	0.0	1.2296064644510524E-4	0.0
14-15	0.0	0.0	0.0	1.2296064644510524E-4	0.0
16-17	0.0	0.0	0.0	1.2296064644510524E-4	0.0
18-19	0.0	0.0	0.0	1.2296064644510524E-4	0.0
20-21	0.0	0.0	0.0	1.4345408751928945E-4	0.0
22-23	0.0	0.0	0.0	1.6394752859347366E-4	0.0
24-25	0.0	0.0	0.0	2.254278518160263E-4	0.0
26-27	0.0	0.0	0.0	3.278950571869473E-4	0.0
28-29	0.0	0.0	0.0	9.426982894124736E-4	0.0
30-31	0.0	0.0	0.0	0.004365102948801236	0.0
32-33	0.0	0.0	0.0	0.01125089914972713	0.0
34-35	0.0	0.0	0.0	0.02278870647449284	0.0
36-37	0.0	0.0	0.0	0.04293375905041591	0.0
38-39	0.0	0.0	0.0	0.07613313359059433	0.0
40-41	0.0	0.0	0.0	0.11621830433169864	0.0
42-43	0.0	0.0	0.0	0.1576765356247733	0.0
44-45	0.0	0.0	0.0	0.20429911406854237	0.0
46-47	0.0	0.0	0.0	0.2612503868137003	0.0
48-49	0.0	0.0	0.0	0.32373488864888794	0.0
50-51	0.0	0.0	0.0	0.3946217013244911	0.0
52-53	0.0	0.0	0.0	0.4680087138111447	0.0
54-55	0.0	0.0	0.0	0.5452075063375966	0.0
56-57	0.0	0.0	0.0	0.6363213453534197	0.0
58-59	0.0	0.0	0.0	0.7444242470197413	0.0
60-61	0.0	0.0	0.0	0.8557036320525615	0.0
62-63	0.0	0.0	0.0	0.9696676578661	0.0
64-65	0.0	0.0	0.0	1.0887755373892585	0.0
66-67	0.0	0.0	0.0	1.224995440209361	0.0
68-69	0.0	0.0	0.0	1.3826309889519859	0.0
70-71	0.0	0.0	0.0	1.5538331956857208	0.0
72-73	0.0	0.0	0.0	1.733622154229539	0.0
74-75	0.0	0.0	0.0	1.9116076899588288	0.0
76-77	0.0	0.0	0.0	2.10336481808997	0.0
78-79	0.0	0.0	0.0	2.3254317455698303	0.0
80-81	0.0	0.0	0.0	2.5681970485346164	0.0
82-83	0.0	0.0	0.0	2.826742301126524	0.0
84-85	0.0	0.0	0.0	3.080000245921293	0.0
86-87	0.0	0.0	0.0	3.348443830552032	0.0
88	0.0	0.0	0.0	3.572990464401868	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2840	0.0	33.49951	1
TCAACGC	2695	0.0	32.42439	4
ATCAACG	2770	0.0	31.383947	3
CTATGCG	95	3.771483E-5	29.663986	9
CAACGCA	2970	0.0	29.421528	5
AACGCAG	3055	0.0	28.287247	6
GTACATG	6200	0.0	27.879168	1
GGTATCA	1820	0.0	27.430891	1
TATCAAC	3415	0.0	25.882456	2
TACATGG	6395	0.0	25.290432	2
ACATGGG	6470	0.0	24.548674	3
ACGCAGA	3625	0.0	23.58068	7
GCGTATA	120	1.813005E-4	23.549162	1
CGCAGAG	3670	0.0	22.908087	8
CATGGGG	4125	0.0	19.703339	4
GCAGAGT	4720	0.0	17.513485	9
GAGTACA	4450	0.0	16.82839	1
ACACCGT	420	5.638867E-11	16.77359	6
GTATTAG	1200	0.0	16.484413	1
TACACCG	285	9.640462E-7	16.484047	5
>>END_MODULE