FastQCFastQC Report
Wed 25 May 2016
SRR1033126_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033126_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2439805
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44230.1812849797422335No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35190.14423283828010847No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31500.051.0739561
GTATCAA59400.043.6364171
TATACCG1156.184564E-1140.873215
TCAACGC70550.036.2441984
ATCAACG70650.036.0598343
CAACGCA72250.035.1962175
TATCAAC76350.033.6755642
AACGCAG76150.033.4492076
ACGCAGA86050.029.491677
CGCAGAG87150.029.1182358
GTACATG61000.027.7623731
TACATGG60950.026.3747882
ACATGGG61250.025.0944823
GCAGAGT101450.024.735919
ATACCGA1852.8200338E-722.8626866
GAGTACT69600.021.9438512-13
AGAGTAC91600.020.98294810-11
CAGAGTA98750.020.17750410-11
CATGGGG39750.019.7476734
AGTACTT74200.019.72844312-13
Produced by FastQC (version 0.11.5)