##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033126_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2439805 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.765691930297706 34.0 31.0 34.0 31.0 34.0 2 32.86698322201979 34.0 31.0 34.0 31.0 34.0 3 32.96639813427713 34.0 31.0 34.0 31.0 34.0 4 36.37990413168266 37.0 37.0 37.0 35.0 37.0 5 36.318319701779444 37.0 37.0 37.0 35.0 37.0 6 36.403123610288525 37.0 37.0 37.0 35.0 37.0 7 36.3747233897791 37.0 37.0 37.0 35.0 37.0 8 36.37517096653216 37.0 37.0 37.0 35.0 37.0 9 38.19734077108621 39.0 39.0 39.0 37.0 39.0 10-11 38.1821407858415 39.0 39.0 39.0 37.0 39.0 12-13 38.14373628220288 39.0 39.0 39.0 37.0 39.0 14-15 39.68363373302374 41.0 40.0 41.0 37.0 41.0 16-17 39.699381098079556 41.0 40.0 41.0 37.0 41.0 18-19 39.71511329798898 41.0 40.0 41.0 37.0 41.0 20-21 39.7106608929812 41.0 40.0 41.0 37.0 41.0 22-23 39.64231690647408 41.0 40.0 41.0 37.0 41.0 24-25 39.59646775049646 41.0 40.0 41.0 37.0 41.0 26-27 39.496952830246684 41.0 39.5 41.0 36.5 41.0 28-29 39.40954010668885 41.0 39.0 41.0 36.0 41.0 30-31 39.32099696492138 41.0 39.0 41.0 36.0 41.0 32-33 39.3577246952113 41.0 39.0 41.0 36.0 41.0 34-35 39.30447986621881 41.0 39.0 41.0 35.5 41.0 36-37 39.222042130416156 41.0 39.0 41.0 35.0 41.0 38-39 39.13559628740822 41.0 39.0 41.0 35.0 41.0 40-41 39.026544949288976 41.0 39.0 41.0 35.0 41.0 42-43 38.92245179430323 41.0 39.0 41.0 35.0 41.0 44-45 38.788320582997414 41.0 39.0 41.0 35.0 41.0 46-47 38.59254878975983 41.0 39.0 41.0 35.0 41.0 48-49 38.41691877014762 41.0 39.0 41.0 35.0 41.0 50-51 38.29404276161415 41.0 38.5 41.0 34.5 41.0 52-53 38.16720147716723 40.5 38.0 41.0 34.0 41.0 54-55 38.03545980109066 40.0 38.0 41.0 34.0 41.0 56-57 37.88571894065304 40.0 37.5 41.0 34.0 41.0 58-59 37.70651916854011 40.0 37.0 41.0 34.0 41.0 60-61 37.52222144802556 40.0 37.0 41.0 33.5 41.0 62-63 37.29763792598179 40.0 36.0 41.0 33.0 41.0 64-65 37.05088992767865 39.0 36.0 41.0 33.0 41.0 66-67 36.78269656796343 39.0 35.0 41.0 33.0 41.0 68-69 36.467933297947994 38.5 35.0 41.0 32.0 41.0 70-71 36.134322005242225 37.5 35.0 40.0 32.0 41.0 72-73 35.78619397861714 37.0 35.0 39.5 32.0 41.0 74-75 35.45235500378104 36.5 35.0 39.0 31.0 41.0 76-77 35.107397722358954 36.0 35.0 39.0 31.0 41.0 78-79 34.7599625789766 36.0 35.0 38.5 31.0 39.5 80-81 34.41234811798484 35.0 35.0 37.0 31.0 39.0 82-83 34.076699572301884 35.0 35.0 37.0 31.0 39.0 84-85 33.79476249126467 35.0 34.0 36.0 31.0 38.0 86-87 33.55016282038933 35.0 34.0 36.0 30.0 37.0 88-89 33.31970259918313 35.0 34.0 36.0 30.0 37.0 90-91 33.113993126499864 35.0 34.0 35.0 30.0 36.5 92-93 32.94664286695043 35.0 34.0 35.0 30.0 36.0 94-95 32.792379718870976 35.0 34.0 35.0 30.0 36.0 96-97 32.665555034111335 35.0 34.0 35.0 29.5 36.0 98-99 32.55673691135152 35.0 34.0 35.0 29.5 36.0 100 32.460955691131055 35.0 34.0 35.0 29.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.12360071169572251 1101 2 0.14707328805581454 1101 3 0.1412905473090973 1101 4 0.11529272932045842 1101 5 0.11570484495489097 1101 6 0.12637093123753118 1101 7 0.12448620173462643 1101 8 0.17410914743047812 1101 9 0.17296439874510128 1101 10-11 0.1942198081076114 1101 12-13 0.20276700874359932 1101 14-15 0.2418319713975734 1101 16-17 0.21793862624603122 1101 18-19 0.22061164547439205 1101 20-21 0.2401707933156274 1101 22-23 0.260241428963603 1101 24-25 0.1903655045606314 1101 26-27 0.15144920450609334 1101 28-29 0.16293711239931952 1101 30-31 0.22441894408983387 1101 32-33 0.18374242692028986 1101 34-35 0.16855833992551084 1101 36-37 0.2656546282931487 1101 38-39 0.24762124634068527 1101 40-41 0.3071312518733791 1101 42-43 0.1743655184109798 1101 44-45 0.12812449499865863 1101 46-47 0.17969413936526735 1101 48-49 0.0918540250628368 1101 50-51 0.1596415828919362 1101 52-53 0.19009212198942294 1101 54-55 0.2026729639058118 1101 56-57 0.22825575062834957 1101 58-59 0.14678957784179403 1101 60-61 0.10484641103411718 1101 62-63 0.19939872825367644 1101 64-65 0.22594163321117122 1101 66-67 0.20002305411556165 1101 68-69 0.16336040008207675 1101 70-71 0.15431537555602404 1101 72-73 0.19371481077524066 1101 74-75 0.12426884584510134 1101 76-77 0.14511162893376905 1101 78-79 0.19685939689507848 1101 80-81 0.20236303879584483 1101 82-83 0.23911551903666606 1101 84-85 0.2096705598210704 1101 86-87 0.13814902793341588 1101 88-89 0.1540977099526657 1101 90-91 0.18713315528373897 1101 92-93 0.21602749830496748 1101 94-95 0.1544882850909346 1101 96-97 0.20740818258662586 1101 98-99 0.1878733729758153 1101 100 0.17305326634762963 1102 1 0.10424091251049816 1102 2 0.08811057173174675 1102 3 0.09714103227435089 1102 4 0.08505235147889323 1102 5 0.0876432451616509 1102 6 0.07832578940431745 1102 7 0.10536817205670701 1102 8 0.13529031724222307 1102 9 0.18668993978756987 1102 10-11 0.12415077802948105 1102 12-13 0.10266777692579865 1102 14-15 0.13587801911009478 1102 16-17 0.14312289229794573 1102 18-19 0.12296662915041878 1102 20-21 0.14337374226397515 1102 22-23 0.15623083847378183 1102 24-25 0.164890112409104 1102 26-27 0.19847562108783023 1102 28-29 0.1301491218695574 1102 30-31 0.13927972997471016 1102 32-33 0.16320688628407964 1102 34-35 0.17191186537786507 1102 36-37 0.17730654416737934 1102 38-39 0.1395909460607072 1102 40-41 0.2438226054800836 1102 42-43 0.16902026861562547 1102 44-45 0.17983490405330116 1102 46-47 0.25816740425631224 1102 48-49 0.18663903863805587 1102 50-51 0.15660083647504308 1102 52-53 0.21785032136411786 1102 54-55 0.1837958393465371 1102 56-57 0.20793492413449144 1102 58-59 0.16902476589386595 1102 60-61 0.23823951684307332 1102 62-63 0.27661409155190597 1102 64-65 0.18042331551895074 1102 66-67 0.16450139123924146 1102 68-69 0.16959739643522198 1102 70-71 0.19194424509989716 1102 72-73 0.15540437077547864 1102 74-75 0.19793057771693157 1102 76-77 0.17914732127017885 1102 78-79 0.19402117674046337 1102 80-81 0.17831112188434162 1102 82-83 0.20153730266808623 1102 84-85 0.23637321098752295 1102 86-87 0.27290108236915955 1102 88-89 0.29166641804602733 1102 90-91 0.24929640026790167 1102 92-93 0.1918288081995314 1102 94-95 0.18621497922788421 1102 96-97 0.18729731609750644 1102 98-99 0.19087781506911483 1102 100 0.23488998851669152 1103 1 0.08506509826388253 1103 2 0.14638258981028685 1103 3 0.10169540036856972 1103 4 0.07818522751292534 1103 5 0.0821377502375995 1103 6 0.0926981488884806 1103 7 0.10103126190928435 1103 8 0.1500157103066897 1103 9 0.1383927143731114 1103 10-11 0.15306651861221354 1103 12-13 0.13872720402142846 1103 14-15 0.17330322809036858 1103 16-17 0.2077522414359123 1103 18-19 0.18118407300271855 1103 20-21 0.19612377989124496 1103 22-23 0.1958514750315814 1103 24-25 0.2124717484882055 1103 26-27 0.21187030071740764 1103 28-29 0.1589288253918255 1103 30-31 0.21219496516907554 1103 32-33 0.23031531474268263 1103 34-35 0.2376777510919723 1103 36-37 0.19096784469007133 1103 38-39 0.19507045006195511 1103 40-41 0.32373072446381457 1103 42-43 0.2002931459550794 1103 44-45 0.27285346365280105 1103 46-47 0.2579928170698551 1103 48-49 0.25168123907085516 1103 50-51 0.16819456663893106 1103 52-53 0.16686582916953796 1103 54-55 0.1358679233000757 1103 56-57 0.18277398260198652 1103 58-59 0.2590746721570767 1103 60-61 0.21101083716013846 1103 62-63 0.15834066905745914 1103 64-65 0.14179529227040888 1103 66-67 0.14554425142871708 1103 68-69 0.2257615322169073 1103 70-71 0.24513465184860905 1103 72-73 0.26789938526680857 1103 74-75 0.2364296950490825 1103 76-77 0.14311234980398524 1103 78-79 0.20730901505243082 1103 80-81 0.23185665960867397 1103 82-83 0.1989332185863546 1103 84-85 0.1369634264202233 1103 86-87 0.19887150104114681 1103 88-89 0.16956860492462056 1103 90-91 0.16808529541541617 1103 92-93 0.12792717134189502 1103 94-95 0.11701871957168208 1103 96-97 0.1898062203795945 1103 98-99 0.23085232416535462 1103 100 0.19340426414430567 1104 1 0.08339752956640467 1104 2 0.09634912824814279 1104 3 0.10829348568664443 1104 4 0.060027023191608464 1104 5 0.01577496532819822 1104 6 -0.027184338642427974 1104 7 0.007040002425810599 1104 8 0.08757100695599718 1104 9 0.0804668613058297 1104 10-11 0.08976591225305697 1104 12-13 0.0920004010670894 1104 14-15 0.15006235021454017 1104 16-17 0.1466683622929139 1104 18-19 0.12851646541529504 1104 20-21 0.11523924415624265 1104 22-23 0.13349739336916855 1104 24-25 0.0957046701281925 1104 26-27 -0.0067200276758256905 1104 28-29 0.06856510712359665 1104 30-31 0.02154192186835502 1104 32-33 -0.034830498267950816 1104 34-35 0.013385710244563143 1104 36-37 0.08644907542739588 1104 38-39 0.07167044746929463 1104 40-41 0.07542912154966075 1104 42-43 0.1172391180904242 1104 44-45 0.07932572377472269 1104 46-47 0.11434382966695722 1104 48-49 0.1194718523329854 1104 50-51 0.19596393626522968 1104 52-53 0.14235037365477865 1104 54-55 0.0942059288289272 1104 56-57 0.11551992913479125 1104 58-59 0.08351303644573704 1104 60-61 0.07640279115332049 1104 62-63 0.07783859168589657 1104 64-65 0.10485522654639823 1104 66-67 0.0328917704118723 1104 68-69 0.08226660573512135 1104 70-71 0.08190607291983554 1104 72-73 0.1350388754195464 1104 74-75 0.08222083008471515 1104 76-77 -0.002418506131427023 1104 78-79 0.040959477032643576 1104 80-81 0.06751947400962166 1104 82-83 0.1481184741095447 1104 84-85 0.17141530496689938 1104 86-87 0.16699166612898608 1104 88-89 0.2112353796189339 1104 90-91 0.196679334558155 1104 92-93 0.24016539335814002 1104 94-95 0.11391530146905637 1104 96-97 0.1450966310898849 1104 98-99 0.19409050405081985 1104 100 0.2597700468207762 1105 1 0.018502192192315192 1105 2 0.04028605078442382 1105 3 0.036433519957391525 1105 4 0.027392218164813187 1105 5 -0.0034007951124124247 1105 6 -0.00927864851595217 1105 7 -0.04124140646903385 1105 8 -0.022528057906534116 1105 9 0.02613815753014137 1105 10-11 -0.038681688016879434 1105 12-13 -0.01846418996965582 1105 14-15 0.016637249843526547 1105 16-17 -0.03815027017348882 1105 18-19 -8.393719634582908E-4 1105 20-21 0.024480918260678664 1105 22-23 0.07745239201637588 1105 24-25 0.03107227213966013 1105 26-27 -0.018538733224133352 1105 28-29 -0.0299350032767407 1105 30-31 -0.039294803813255896 1105 32-33 -0.1001585349768348 1105 34-35 -0.029007162558706057 1105 36-37 -0.11517031087115015 1105 38-39 -0.1204966488775483 1105 40-41 -0.021614909524153347 1105 42-43 -0.05117083722076643 1105 44-45 -0.0080957909178494 1105 46-47 -0.009194959723416218 1105 48-49 -0.172092085009389 1105 50-51 -0.12288124514895316 1105 52-53 -0.11883679533858782 1105 54-55 -0.09760718673067714 1105 56-57 -0.1385893345416349 1105 58-59 -0.11153270972057783 1105 60-61 -0.1490193346284201 1105 62-63 -0.13132794021979777 1105 64-65 -0.20340221303663242 1105 66-67 -0.15138158547837577 1105 68-69 -0.18503973497586657 1105 70-71 -0.20892665658068665 1105 72-73 -0.15245863668561555 1105 74-75 -0.18576167379485042 1105 76-77 -0.12610400357045393 1105 78-79 -0.1743796774563009 1105 80-81 -0.12911401554277546 1105 82-83 -0.1513355884672336 1105 84-85 -0.10501756696559994 1105 86-87 -0.14248204304232814 1105 88-89 -0.14203845674600757 1105 90-91 -0.10475847429066931 1105 92-93 -0.05087923481043077 1105 94-95 -0.05593359001829867 1105 96-97 -0.13671258158469612 1105 98-99 -0.1056039124911905 1105 100 -0.1300892160781828 1106 1 0.08679313688914903 1106 2 0.0756437330524804 1106 3 0.0573024696076061 1106 4 0.03266420354805888 1106 5 0.037479852000032565 1106 6 0.021512266355635745 1106 7 0.035967138225885265 1106 8 0.057374377742512195 1106 9 0.10987164627290014 1106 10-11 0.025851126317370188 1106 12-13 0.056549328323960424 1106 14-15 0.06904330478640475 1106 16-17 0.04181740820534685 1106 18-19 0.09441177004527646 1106 20-21 0.06759268032439536 1106 22-23 0.10065099178063974 1106 24-25 0.10780178607162583 1106 26-27 0.0018800788720270134 1106 28-29 -0.014451710485857916 1106 30-31 0.07072677027982621 1106 32-33 0.14262766959334527 1106 34-35 0.09400822816397181 1106 36-37 0.11643836010387076 1106 38-39 0.10079039855413896 1106 40-41 0.1273908732931659 1106 42-43 0.14912598364641383 1106 44-45 0.15044489996611787 1106 46-47 0.23918098096490326 1106 48-49 0.21402792313887176 1106 50-51 0.16387971503314702 1106 52-53 0.17280883210111853 1106 54-55 0.16715821234306816 1106 56-57 0.15854861230022266 1106 58-59 0.14288985260225928 1106 60-61 0.1334618888867638 1106 62-63 0.21292703850883754 1106 64-65 0.2030015361163251 1106 66-67 0.22020621975320864 1106 68-69 0.23662205178316498 1106 70-71 0.23508162260987575 1106 72-73 0.27248075841063013 1106 74-75 0.23591126015057995 1106 76-77 0.2210504739150636 1106 78-79 0.24313856217406027 1106 80-81 0.21258497672175025 1106 82-83 0.2155108971045081 1106 84-85 0.16762952668329945 1106 86-87 0.17940660946489828 1106 88-89 0.1966828200489914 1106 90-91 0.2550778477313429 1106 92-93 0.18616803283896388 1106 94-95 0.15425395412306386 1106 96-97 0.2385881314773357 1106 98-99 0.30691438529839843 1106 100 0.3568272678591313 1107 1 -0.004683466311050211 1107 2 0.01728137121781259 1107 3 0.0049467248930312735 1107 4 0.017643102612716177 1107 5 -0.1128086097592984 1107 6 -0.1308206805052663 1107 7 -0.14522088549306744 1107 8 -0.052920719513018355 1107 9 -0.0585358690941149 1107 10-11 -0.0611582173571108 1107 12-13 0.019462438927433823 1107 14-15 0.0032187015379747663 1107 16-17 0.021942001059805705 1107 18-19 -0.009689898332659652 1107 20-21 -0.04828828587965717 1107 22-23 0.015349477245138132 1107 24-25 -0.028760551701836334 1107 26-27 -0.05447699064477263 1107 28-29 -0.013120555582197824 1107 30-31 0.03753669159564055 1107 32-33 0.020901882119879645 1107 34-35 0.05801450159350452 1107 36-37 0.09100385856716997 1107 38-39 0.05977382244721241 1107 40-41 0.10474817566965555 1107 42-43 0.14304349331683852 1107 44-45 0.18511375252774798 1107 46-47 0.11327947515805192 1107 48-49 0.1774272108925743 1107 50-51 0.16390598711892324 1107 52-53 0.1874983376805588 1107 54-55 0.17287752338848605 1107 56-57 0.10823178960895063 1107 58-59 0.1137949532343967 1107 60-61 0.10024345644880128 1107 62-63 0.13664334563554803 1107 64-65 0.15050832953216542 1107 66-67 0.11301901692926464 1107 68-69 0.17131678880962653 1107 70-71 0.18029387952847742 1107 72-73 0.1521919320004983 1107 74-75 0.19587776970156057 1107 76-77 0.14113200926149716 1107 78-79 0.15259881724586677 1107 80-81 0.13072022427083851 1107 82-83 0.1136679820687192 1107 84-85 0.021185152010616548 1107 86-87 0.04788040083411005 1107 88-89 0.08674867684631238 1107 90-91 0.08362614415167968 1107 92-93 0.09256755402660843 1107 94-95 0.07694532688692846 1107 96-97 0.05034928290102414 1107 98-99 0.03672400025011768 1107 100 0.08130465434074807 1108 1 0.051358968681334716 1108 2 0.052260729569191255 1108 3 0.024578819269599705 1108 4 0.032230686212415094 1108 5 -0.01648986505012573 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-0.16220422135251766 2214 24-25 -0.1481370035506373 2214 26-27 -0.18097227019205775 2214 28-29 -0.13501246936394296 2214 30-31 -0.12483884116463884 2214 32-33 -0.08707693306586606 2214 34-35 -0.14419113477845258 2214 36-37 -0.21158490742443803 2214 38-39 -0.23477702732817818 2214 40-41 -0.1490634593101703 2214 42-43 -0.1483025781373044 2214 44-45 -0.18598965842923576 2214 46-47 -0.13397675803453524 2214 48-49 -0.25413561520484507 2214 50-51 -0.1853265211475872 2214 52-53 -0.23809745587549003 2214 54-55 -0.2076089831478427 2214 56-57 -0.22196425913207918 2214 58-59 -0.30362734974553973 2214 60-61 -0.33087273723355537 2214 62-63 -0.4323666446101342 2214 64-65 -0.2878901440531294 2214 66-67 -0.3396162317000204 2214 68-69 -0.31239961545315253 2214 70-71 -0.23025081247122614 2214 72-73 -0.43694967688566777 2214 74-75 -0.28332389099630717 2214 76-77 -0.24737421952932692 2214 78-79 -0.35735973573554247 2214 80-81 -0.3296870739727993 2214 82-83 -0.3145468616216718 2214 84-85 -0.25276220992362397 2214 86-87 -0.1934941940567967 2214 88-89 -0.3504918391109655 2214 90-91 -0.18774823945450692 2214 92-93 -0.2203729892803068 2214 94-95 -0.23754845253887424 2214 96-97 -0.3986096116573421 2214 98-99 -0.4808956778693556 2214 100 -0.5879490763017543 2215 1 -0.05200799984661586 2215 2 -0.07678354778715857 2215 3 -0.0671477362901598 2215 4 -0.03520863309273636 2215 5 -0.02076429649196143 2215 6 -0.04637029209804666 2215 7 -0.04743757368098045 2215 8 -0.08549124428977706 2215 9 -0.05108371116234167 2215 10-11 -0.10041143017146936 2215 12-13 -0.13823704251305458 2215 14-15 -0.15332871010535598 2215 16-17 -0.1240600007575523 2215 18-19 -0.12541185068530325 2215 20-21 -0.1576298724394718 2215 22-23 -0.15106183814699392 2215 24-25 -0.14884141009205365 2215 26-27 -0.09325195358040617 2215 28-29 -0.11567868002281045 2215 30-31 -0.13451541031257364 2215 32-33 -0.12995264793129735 2215 34-35 -0.15320199529445233 2215 36-37 -0.05704009942882493 2215 38-39 -0.11446627265618758 2215 40-41 -0.18730536098819783 2215 42-43 -0.16331056873266903 2215 44-45 -0.1064743445159877 2215 46-47 -0.1450155981849761 2215 48-49 0.0029889836965537597 2215 50-51 -0.005732660936985212 2215 52-53 -0.11222256935450048 2215 54-55 -0.0734621570039522 2215 56-57 -0.18414541973467635 2215 58-59 -0.17681273155794486 2215 60-61 -0.15541014033189526 2215 62-63 -0.1904607830273335 2215 64-65 -0.08167003034403564 2215 66-67 -0.10736706736568635 2215 68-69 -0.0877289660394851 2215 70-71 -0.02317411530642488 2215 72-73 0.057806756380308855 2215 74-75 0.03520801490710568 2215 76-77 -0.061886772294450054 2215 78-79 -0.04140751614895777 2215 80-81 -0.007568290216717344 2215 82-83 -0.029452564229288214 2215 84-85 -0.04412243098281721 2215 86-87 -0.006655457658588659 2215 88-89 -0.0022749760262641416 2215 90-91 -0.03951380542712002 2215 92-93 -0.062484472728058904 2215 94-95 -0.043422481430525295 2215 96-97 0.01158456817707787 2215 98-99 -0.05945034232709645 2215 100 -0.112518065261348 2216 1 -0.029811238145526886 2216 2 -0.09106697653469098 2216 3 -0.10294563279057911 2216 4 -0.08886719975598112 2216 5 -0.06302314126747177 2216 6 -0.04811482343347251 2216 7 -0.04629676117496473 2216 8 -0.0891580286008562 2216 9 -0.11746238910541251 2216 10-11 -0.10721857278073088 2216 12-13 -0.08580681230760234 2216 14-15 -0.18527945420082403 2216 16-17 -0.20412294488430405 2216 18-19 -0.12740539421144348 2216 20-21 -0.09903236846541574 2216 22-23 -0.12878478249638192 2216 24-25 -0.1673202318057534 2216 26-27 -0.23689690164771093 2216 28-29 -0.24463242721565592 2216 30-31 -0.18855397498087711 2216 32-33 -0.1620724922303367 2216 34-35 -0.19695357634589072 2216 36-37 -0.2710597886074808 2216 38-39 -0.1511847530657775 2216 40-41 -0.16965869889197904 2216 42-43 -0.20103925779629606 2216 44-45 -0.03911792472142395 2216 46-47 -0.0740878301090433 2216 48-49 -0.021452680061742058 2216 50-51 -0.045909428762570315 2216 52-53 -0.036770756964813245 2216 54-55 0.015989504346890726 2216 56-57 -0.04098299928926252 2216 58-59 -0.018384693380035344 2216 60-61 -0.003435984124998015 2216 62-63 0.021692419683596142 2216 64-65 0.07407420371553286 2216 66-67 0.06346729999437883 2216 68-69 0.08272736769349365 2216 70-71 0.1389351944700934 2216 72-73 0.1410632879916207 2216 74-75 0.08630732447190326 2216 76-77 0.06578017091297994 2216 78-79 0.047823616491740495 2216 80-81 0.040934793747567255 2216 82-83 0.014220932797847752 2216 84-85 0.043755190670566435 2216 86-87 0.008387257740373855 2216 88-89 -0.017242963213270457 2216 90-91 -0.0014923694799193754 2216 92-93 -0.004256817472899854 2216 94-95 -0.01086087035247374 2216 96-97 -0.02129434450916534 2216 98-99 -0.03573555298372355 2216 100 -0.005361628966014109 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 18.0 3 3.0 4 6.0 5 18.0 6 43.0 7 147.0 8 208.0 9 514.0 10 923.0 11 1454.0 12 2360.0 13 3505.0 14 4326.0 15 5907.0 16 7088.0 17 10100.0 18 11863.0 19 6790.0 20 4506.0 21 4195.0 22 4243.0 23 4694.0 24 5351.0 25 6524.0 26 8062.0 27 10285.0 28 13289.0 29 17128.0 30 22063.0 31 28548.0 32 36498.0 33 47457.0 34 65859.0 35 101464.0 36 188636.0 37 450805.0 38 878424.0 39 484179.0 40 2322.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.61647376589109 20.135797004359603 13.70535635534657 23.542372874402744 2 15.364187868451587 20.323259846928256 40.67258771417481 23.63996457044534 3 18.18247769801275 25.543598771213272 30.27754267246768 25.9963808583063 4 12.02471021202139 16.88699201287318 39.39911492781583 31.689182847289594 5 12.384162305006633 37.950399058578476 36.13106916536996 13.534369471044933 6 30.493716495730595 38.694098412124596 17.655251391404462 13.156933700740348 7 26.761560964332865 32.78771913069533 22.770040705874543 17.680679199097266 8 24.808334778123296 36.597206083610914 20.421747511374903 18.17271162689088 9 24.73880063267403 16.590847703687555 21.67642223572969 36.993929427908725 10-11 23.579635346561762 26.923296046062866 29.156515544742266 20.340553062633106 12-13 24.56442215668875 24.208225657378357 29.51125192382178 21.716100262111112 14-15 21.582368911601097 26.203498313798978 26.963887263075232 25.250245511524692 16-17 20.748604908998875 29.79545496463857 27.39712804916786 22.058812077194695 18-19 20.558988098007152 28.28818246074527 30.221106102279222 20.931723338968354 20-21 21.67353538500003 27.531687983260955 29.9842815306961 20.81049510104291 22-23 21.225018444134765 27.34236412820723 30.091380441019755 21.341236986638247 24-25 21.136378633848715 27.472709886822827 29.986068547337634 21.40484293199082 26-27 20.950080970714055 27.448600708837017 30.085712962405076 21.51560535804385 28-29 20.961553399331738 27.514910716060736 30.109798534967453 21.413737349640076 30-31 21.183015652778597 27.984425944513326 29.68915031522046 21.143408087487618 32-33 20.875800548994736 27.69336321489284 30.166094378635822 21.264741857476604 34-35 21.037644453245466 27.466154676847427 30.136881839325884 21.359319030581226 36-37 21.320561023916614 27.89716884710987 29.547557522334323 21.23471260663919 38-39 21.20233042378833 27.99247134219111 29.62045545018004 21.184742783840527 40-41 21.06656378577624 27.751088377459165 29.797650043572 21.3846977931926 42-43 21.116459032442346 27.62909572574203 29.68086089849588 21.57358434331974 44-45 21.194680760350526 27.867835637823372 29.502405612111037 21.435077989715065 46-47 21.35777620181764 28.11501047375821 29.170564435134438 21.356648889289712 48-49 21.318209021298923 27.998594008755678 29.026566266047976 21.656630703897424 50-51 21.41708402853794 27.94653860350516 28.89241993507621 21.74395743288069 52-53 21.433892660432946 28.0192852568208 28.836152171128475 21.710669911617778 54-55 21.50842866224729 28.422403309244416 28.62769401785703 21.441474010651262 56-57 21.32206002634291 28.08641311153151 28.754577065517694 21.836949796607886 58-59 21.206381137350448 27.748803224219188 29.125051823224553 21.91976381520581 60-61 21.50257071094585 28.08173811538033 28.736056188396386 21.67963498527743 62-63 21.268200731093057 28.016250702166168 28.84747608536291 21.86807248137787 64-65 21.301970872210553 27.77100107716749 28.977711486343583 21.94931656427837 66-67 21.21325891443637 27.905569838418636 28.766257786266657 22.114913460878334 68-69 21.640613120101253 28.01997144828044 28.709479490594042 21.629935941024264 70-71 21.570073247897266 27.828380803812774 28.73333314206059 21.86821280622937 72-73 21.37866529332696 27.942886173405178 28.797199928108697 21.881248605159165 74-75 21.471161391322667 28.07348244841213 28.617913770161284 21.837442390103917 76-77 21.47174099489814 28.05058616545021 28.478121647390818 21.99955119226083 78-79 21.530725418927094 28.175972332203735 28.537435794022798 21.755866454846373 80-81 21.516654189507587 27.980034359493423 28.62746673230069 21.875844718698296 82-83 21.35377003140429 28.063824240899027 28.766055658797423 21.81635006889926 84-85 21.349784152168365 27.95904168276261 28.57228929859958 22.11888486646945 86-87 21.498859640835537 28.07275509913409 28.483690169056786 21.94469509097359 88-89 21.218109644008436 28.00502499175139 28.547957726129752 22.228907638110424 90-91 21.15204312476676 27.889919048841822 28.67520737829609 22.28283044809533 92-93 21.344783977568692 28.061818067800104 28.449820467172675 22.143577487458526 94-95 21.063076762282233 27.88151102239728 28.62919372654782 22.42621848877267 96-97 21.154190601298055 27.787507608189998 28.61064716237568 22.447654628136267 98-99 21.068097655345408 27.942315062064388 28.55668793202735 22.432899350562852 100 21.205301243337072 27.85603767514207 28.37903029135525 22.559630790165606 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 22.0 1 25.5 2 95.5 3 184.5 4 141.5 5 104.0 6 250.0 7 573.5 8 983.0 9 1197.5 10 1006.5 11 761.0 12 814.0 13 994.0 14 1124.0 15 1300.0 16 1493.5 17 1692.0 18 2003.5 19 2391.0 20 3005.5 21 3886.0 22 5075.5 23 7139.0 24 9798.0 25 12832.0 26 16849.5 27 22294.0 28 29275.5 29 36690.0 30 43836.5 31 51575.5 32 58910.5 33 65601.0 34 72518.5 35 77885.5 36 81481.5 37 85178.5 38 88764.5 39 89496.5 40 89155.5 41 89788.0 42 88742.0 43 87283.0 44 85571.0 45 83203.0 46 81702.0 47 79075.5 48 75890.0 49 73338.0 50 71062.5 51 68197.0 52 63555.0 53 59774.0 54 57294.0 55 53904.5 56 49594.5 57 45454.0 58 41481.5 59 37810.5 60 34092.0 61 29314.0 62 24918.5 63 20882.0 64 16931.5 65 13480.0 66 10448.5 67 7775.5 68 5724.0 69 4310.0 70 3066.0 71 2218.0 72 1642.5 73 1223.5 74 915.5 75 661.5 76 418.5 77 271.5 78 181.0 79 96.5 80 55.5 81 24.5 82 13.0 83 9.5 84 3.5 85 1.5 86 2.0 87 2.5 88 3.0 89 2.5 90 0.5 91 0.5 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09000719319781704 2 0.001393553993044526 3 0.0 4 1.6394752859347366E-4 5 0.038527669219466314 6 1.6394752859347366E-4 7 0.0044265832720237885 8 1.6394752859347366E-4 9 8.197376429673683E-5 10-11 3.6888193933531574E-4 12-13 0.0 14-15 4.0986882148368415E-5 16-17 0.0 18-19 1.4345408751928945E-4 20-21 0.0 22-23 2.0493441074184208E-4 24-25 8.197376429673683E-5 26-27 0.001393553993044526 28-29 0.008217869870747867 30-31 0.0359454956441191 32-33 0.02539137349091423 34-35 0.028444896210967678 36-37 0.025493840696285158 38-39 0.024264234231834103 40-41 0.030268812466570073 42-43 0.02211242291904476 44-45 0.020862323013519524 46-47 0.015370080805638156 48-49 0.01004178612635026 50-51 0.0071931978170386565 52-53 0.004815958652433289 54-55 0.0038527669219466307 56-57 0.0024387194878279206 58-59 8.402310840415525E-4 60-61 5.328294679287894E-4 62-63 0.0013730605519703418 64-65 0.002992042396830894 66-67 5.123360268546052E-4 68-69 9.017114072641052E-4 70-71 3.278950571869473E-4 72-73 4.3036226255786835E-4 74-75 4.7134914470623677E-4 76-77 3.0740161611276314E-4 78-79 4.0986882148368415E-5 80-81 5.533229090029736E-4 82-83 4.508557036320526E-4 84-85 3.0740161611276314E-4 86-87 1.4345408751928945E-4 88-89 0.0 90-91 2.0493441074184208E-5 92-93 2.0493441074184208E-5 94-95 0.0 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2439805.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.8618706201566 #Duplication Level Percentage of deduplicated Percentage of total 1 78.04093948849207 52.17963199155026 2 13.8392957469421 18.506424034122517 3 4.033260516221569 8.090140284389774 4 1.5907438454478902 4.254404367365887 5 0.8138289908646709 2.720706434706311 6 0.43979865483740305 1.7643456455194408 7 0.2627304082146418 1.2296652593419812 8 0.19984383142700343 1.068954592088695 9 0.13762113829291556 0.8281446068825637 >10 0.6086982312155773 6.937324098557357 >50 0.02603749796363015 1.1678224531016113 >100 0.006833456235732264 0.7599915876224542 >500 1.2273128161279526E-4 0.04451778366690364 >1k 2.454625632180034E-4 0.4479268610844145 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4423 0.1812849797422335 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3519 0.14423283828010847 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 8.197376429673683E-5 0.0 10-11 0.0 0.0 0.0 8.197376429673683E-5 0.0 12-13 0.0 0.0 0.0 8.197376429673683E-5 0.0 14-15 0.0 0.0 0.0 8.197376429673683E-5 0.0 16-17 0.0 0.0 0.0 8.197376429673683E-5 0.0 18-19 0.0 0.0 0.0 8.197376429673683E-5 0.0 20-21 0.0 0.0 0.0 1.0246720537092103E-4 0.0 22-23 0.0 0.0 0.0 1.2296064644510524E-4 0.0 24-25 0.0 0.0 0.0 2.0493441074184205E-4 0.0 26-27 0.0 0.0 0.0 3.278950571869473E-4 0.0 28-29 0.0 0.0 0.0 9.426982894124736E-4 0.0 30-31 0.0 0.0 0.0 0.00438559638987542 0.0 32-33 0.0 0.0 0.0 0.011271392590801314 0.0 34-35 0.0 0.0 0.0 0.02278870647449284 0.0 36-37 0.0 0.0 0.0 0.04289277216826755 0.0 38-39 0.0 0.0 0.0 0.07611264014952014 0.0 40-41 0.0 0.0 0.0 0.1161363305674019 0.0 42-43 0.0 0.0 0.0 0.15763554874262492 0.0 44-45 0.0 0.0 0.0 0.20446306159713584 0.0 46-47 0.0 0.0 0.0 0.2616192687530356 0.0 48-49 0.0 0.0 0.0 0.32439067876326183 0.0 50-51 0.0 0.0 0.0 0.3953799586442359 0.0 52-53 0.0 0.0 0.0 0.46923832027559575 0.0 54-55 0.0 0.0 0.0 0.5472363570039409 0.0 56-57 0.0 0.0 0.0 0.6385141435483574 0.0 58-59 0.0 0.0 0.0 0.7471908615647562 0.0 60-61 0.0 0.0 0.0 0.8591260367119504 0.0 62-63 0.0 0.0 0.0 0.9738483198452335 0.0 64-65 0.0 0.0 0.0 1.0931201468969856 0.0 66-67 0.0 0.0 0.0 1.2306311365047615 0.0 68-69 0.0 0.0 0.0 1.3892093835367991 0.0 70-71 0.0 0.0 0.0 1.5607804722098693 0.0 72-73 0.0 0.0 0.0 1.740774365164429 0.0 74-75 0.0 0.0 0.0 1.9198460532706507 0.0 76-77 0.0 0.0 0.0 2.1131606829234304 0.0 78-79 0.0 0.0 0.0 2.3376253430089697 0.0 80-81 0.0 0.0 0.0 2.582337522875804 0.0 82-83 0.0 0.0 0.0 2.843157547426946 0.0 84-85 0.0 0.0 0.0 3.099141119884581 0.0 86-87 0.0 0.0 0.0 3.3712120435854507 0.0 88 0.0 0.0 0.0 3.598689239508895 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3150 0.0 51.073956 1 GTATCAA 5940 0.0 43.636417 1 TATACCG 115 6.184564E-11 40.87321 5 TCAACGC 7055 0.0 36.244198 4 ATCAACG 7065 0.0 36.059834 3 CAACGCA 7225 0.0 35.196217 5 TATCAAC 7635 0.0 33.675564 2 AACGCAG 7615 0.0 33.449207 6 ACGCAGA 8605 0.0 29.49167 7 CGCAGAG 8715 0.0 29.118235 8 GTACATG 6100 0.0 27.762373 1 TACATGG 6095 0.0 26.374788 2 ACATGGG 6125 0.0 25.094482 3 GCAGAGT 10145 0.0 24.73591 9 ATACCGA 185 2.8200338E-7 22.862686 6 GAGTACT 6960 0.0 21.94385 12-13 AGAGTAC 9160 0.0 20.982948 10-11 CAGAGTA 9875 0.0 20.177504 10-11 CATGGGG 3975 0.0 19.747673 4 AGTACTT 7420 0.0 19.728443 12-13 >>END_MODULE