FastQCFastQC Report
Wed 25 May 2016
SRR1033125_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033125_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3036388
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC37100.034.9433174
GTATCAA39150.034.9054151
ATCAACG37950.034.2912143
CAACGCA39450.033.0988165
GGTATCA24050.031.3495581
AACGCAG45500.028.7934076
GTACATG78850.027.6100271
TATCAAC48450.027.5547922
TACATGG77650.026.6974492
ACATGGG79950.025.0913643
CGCAGAG50200.024.8815928
ACGCAGA51900.024.6094427
CATGGGG48100.020.8000074
GAGTACA54750.018.590721
GCAGAGT64750.018.4201939
CCGATCG1300.00808443618.0603799
CTGTCGC7300.018.0108999
TCTGTCG7100.017.8568828
GTATAGA13350.017.6488041
AGAGTAC50350.016.18080910-11
Produced by FastQC (version 0.11.5)