##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033125_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3036388
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.67686277247835	33.0	31.0	34.0	30.0	34.0
2	31.859801843506165	34.0	31.0	34.0	30.0	34.0
3	31.941119514370364	34.0	31.0	34.0	30.0	34.0
4	35.32375144415009	37.0	35.0	37.0	33.0	37.0
5	35.416302527871935	37.0	35.0	37.0	35.0	37.0
6	35.501685884676135	37.0	37.0	37.0	35.0	37.0
7	35.415384002308	37.0	37.0	37.0	35.0	37.0
8	35.41425008925078	37.0	37.0	37.0	35.0	37.0
9	37.09452019965828	39.0	38.0	39.0	35.0	39.0
10-11	37.085452682595246	39.0	38.0	39.0	35.0	39.0
12-13	37.03969008572027	39.0	38.0	39.0	35.0	39.0
14-15	38.52012144034293	41.0	39.0	41.0	35.0	41.0
16-17	38.54056497390979	41.0	39.0	41.0	35.0	41.0
18-19	38.54025753625689	41.0	39.0	41.0	35.0	41.0
20-21	38.57704137284168	41.0	39.0	41.0	34.5	41.0
22-23	38.54778835906347	41.0	39.0	41.0	34.0	41.0
24-25	38.549461070192606	41.0	39.0	41.0	34.0	41.0
26-27	38.515190087696304	41.0	39.0	41.0	34.0	41.0
28-29	38.48965333152417	40.5	39.0	41.0	34.0	41.0
30-31	38.488357219169615	41.0	39.0	41.0	34.0	41.0
32-33	38.554816611052345	41.0	39.0	41.0	34.0	41.0
34-35	38.52724273050743	41.0	39.0	41.0	34.0	41.0
36-37	38.452905722193606	41.0	39.0	41.0	34.0	41.0
38-39	38.38239497060323	41.0	39.0	41.0	34.0	41.0
40-41	38.32730961919228	41.0	39.0	41.0	34.0	41.0
42-43	38.22315642796639	41.0	38.5	41.0	33.0	41.0
44-45	38.11573290370006	40.0	38.0	41.0	33.0	41.0
46-47	38.00554589861375	40.0	38.0	41.0	33.0	41.0
48-49	37.903413694165565	40.0	38.0	41.0	33.0	41.0
50-51	37.79995771291416	40.0	38.0	41.0	33.0	41.0
52-53	37.66600941645139	40.0	38.0	41.0	32.5	41.0
54-55	37.53239391671947	40.0	37.0	41.0	32.0	41.0
56-57	37.39891410452155	40.0	37.0	41.0	32.0	41.0
58-59	37.22268975506424	40.0	37.0	41.0	31.5	41.0
60-61	37.02331651949619	40.0	36.0	41.0	31.0	41.0
62-63	36.779144990692885	39.5	36.0	41.0	31.0	41.0
64-65	36.52418564425891	39.0	35.0	41.0	31.0	41.0
66-67	36.24644808239263	39.0	35.0	41.0	31.0	41.0
68-69	35.94930160440629	38.0	35.0	40.5	30.0	41.0
70-71	35.60677028100493	37.5	35.0	40.0	30.0	41.0
72-73	35.24314893221815	37.0	35.0	39.5	30.0	41.0
74-75	34.868428376083685	36.5	34.5	39.0	29.0	41.0
76-77	34.52820456410709	36.0	34.0	39.0	29.0	40.5
78-79	34.14409538570169	35.5	34.0	38.5	29.0	40.0
80-81	33.762325005895164	35.0	34.0	37.0	29.0	39.0
82-83	33.515048636735486	35.0	34.0	37.0	29.0	39.0
84-85	33.21949829863641	35.0	34.0	36.5	29.0	38.5
86-87	32.93486125620309	35.0	34.0	36.0	29.0	37.0
88-89	32.54629859556816	35.0	34.0	36.0	27.5	37.0
90-91	32.33483154985463	35.0	34.0	35.5	27.0	36.5
92-93	32.138859724119584	35.0	34.0	35.0	27.0	36.0
94-95	31.95771736023196	35.0	34.0	35.0	27.0	36.0
96-97	31.773786979793094	35.0	34.0	35.0	26.0	36.0
98-99	31.577197644042855	35.0	33.0	35.0	25.5	36.0
100	31.41074625508993	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.18580885621642196
1101	2	0.21443992995952144
1101	3	0.25995261641914524
1101	4	0.15632175458619457
1101	5	0.19700696341617174
1101	6	0.18486449897102375
1101	7	0.22237990420676113
1101	8	0.1552495832948111
1101	9	0.22473792749391208
1101	10-11	0.28083815169808446
1101	12-13	0.3023263246034418
1101	14-15	0.35161829735201877
1101	16-17	0.27346523539713985
1101	18-19	0.2416810216223979
1101	20-21	0.25935995418475244
1101	22-23	0.2867714435034543
1101	24-25	0.25148170407691595
1101	26-27	0.31634742348396827
1101	28-29	0.35388737847317486
1101	30-31	0.4113114960029165
1101	32-33	0.39583318201763973
1101	34-35	0.4209916579562787
1101	36-37	0.35394905080937633
1101	38-39	0.38234341633917523
1101	40-41	0.3488263883851843
1101	42-43	0.35354899112431326
1101	44-45	0.389846814489502
1101	46-47	0.3754773326120784
1101	48-49	0.29810399064822235
1101	50-51	0.2930808419335733
1101	52-53	0.2719272420723158
1101	54-55	0.23464935323746516
1101	56-57	0.19970496858735487
1101	58-59	0.272668387139845
1101	60-61	0.25020654154327104
1101	62-63	0.30888798269357665
1101	64-65	0.2969895921362351
1101	66-67	0.23956620113479943
1101	68-69	0.23620910195663214
1101	70-71	0.28659609186657065
1101	72-73	0.3180212177427677
1101	74-75	0.28785711385972945
1101	76-77	0.2568837867341003
1101	78-79	0.21734298396000895
1101	80-81	0.2846496259772735
1101	82-83	0.29400191643102147
1101	84-85	0.21964301653944318
1101	86-87	0.237273884713872
1101	88-89	0.3652968508231069
1101	90-91	0.4519457942834748
1101	92-93	0.45681812953563394
1101	94-95	0.5308024582295303
1101	96-97	0.5003897763562506
1101	98-99	0.5107049225642655
1101	100	0.5721945190501678
1102	1	0.05852410880556036
1102	2	0.12562104554564613
1102	3	0.023052019357240994
1102	4	0.17493516511587615
1102	5	0.06211694008258917
1102	6	0.05907515880478087
1102	7	-0.024269534148416483
1102	8	0.03379974221677173
1102	9	0.06513991331532765
1102	10-11	0.07242385326821221
1102	12-13	0.07986983611104392
1102	14-15	0.13664275274260262
1102	16-17	0.12401535689683385
1102	18-19	0.09790689075298786
1102	20-21	0.058328280633283214
1102	22-23	0.10266336002693777
1102	24-25	0.09757238700067461
1102	26-27	0.12881531720908157
1102	28-29	0.170064582691424
1102	30-31	0.15405082148833316
1102	32-33	0.14796418297812153
1102	34-35	0.14015498747188815
1102	36-37	0.14675864856970122
1102	38-39	0.04636766824621219
1102	40-41	0.05967618575448341
1102	42-43	0.10473228473167495
1102	44-45	0.08676868519410874
1102	46-47	0.04823805667121661
1102	48-49	0.04058272941305319
1102	50-51	0.0070080212939629405
1102	52-53	-0.05140850982676426
1102	54-55	0.004361087164269861
1102	56-57	-0.07736334865963812
1102	58-59	-0.07277131387454716
1102	60-61	-0.07410313040554684
1102	62-63	-0.04669064104914611
1102	64-65	0.02904734358430261
1102	66-67	-0.059039368974808326
1102	68-69	-0.039000794999239474
1102	70-71	-0.04744060123090321
1102	72-73	-0.05926134765362434
1102	74-75	-0.1126040071206802
1102	76-77	-0.12522667819014544
1102	78-79	-0.06580105985644025
1102	80-81	-0.0897235012339479
1102	82-83	-0.17349297066699165
1102	84-85	-0.1891352875395924
1102	86-87	-0.14199385747389925
1102	88-89	0.002547271866326639
1102	90-91	0.06747493164958485
1102	92-93	0.06356050858629203
1102	94-95	0.15828566912571063
1102	96-97	0.16085865675122335
1102	98-99	0.10074890113705237
1102	100	0.09621866941509438
1103	1	0.20666610299371513
1103	2	0.19637553725471335
1103	3	0.2257187992272911
1103	4	0.2486335528299506
1103	5	0.15181232569599246
1103	6	0.2091644944048312
1103	7	0.29912257429334943
1103	8	0.31665962229636335
1103	9	0.3035302651147376
1103	10-11	0.3859506295166355
1103	12-13	0.3540167590684007
1103	14-15	0.44835437795626376
1103	16-17	0.410650857256897
1103	18-19	0.43877247765394145
1103	20-21	0.35665755788053133
1103	22-23	0.3964091715570106
1103	24-25	0.3493894450260271
1103	26-27	0.3479391495504487
1103	28-29	0.4357902351751619
1103	30-31	0.3743708344094401
1103	32-33	0.37974504355820926
1103	34-35	0.3409475548737788
1103	36-37	0.3701905108560197
1103	38-39	0.3125202931607305
1103	40-41	0.31600946735032664
1103	42-43	0.2354232697846328
1103	44-45	0.2663087820758676
1103	46-47	0.3072765840720777
1103	48-49	0.3655103798480823
1103	50-51	0.28162226749761743
1103	52-53	0.2512481160093003
1103	54-55	0.27393805916390335
1103	56-57	0.16141354184239987
1103	58-59	0.13048456026451305
1103	60-61	0.20642624133999732
1103	62-63	0.2747592665640681
1103	64-65	0.29816912521984307
1103	66-67	0.3353473185283562
1103	68-69	0.28982068149569784
1103	70-71	0.3017924458437946
1103	72-73	0.32788949895070374
1103	74-75	0.23288158233177114
1103	76-77	0.28137646744888656
1103	78-79	0.29404018898030415
1103	80-81	0.23681486531639706
1103	82-83	0.29843958244739355
1103	84-85	0.3075021905631772
1103	86-87	0.3273096741842423
1103	88-89	0.40035927376867875
1103	90-91	0.4039376365515821
1103	92-93	0.4206363999579139
1103	94-95	0.4012541802336784
1103	96-97	0.4638059466467368
1103	98-99	0.5474615588893954
1103	100	0.4932407902849114
1104	1	-0.037117059577656875
1104	2	3.4759230370795535E-4
1104	3	-0.020990452284699757
1104	4	-0.013912084028390836
1104	5	-0.040224165953205215
1104	6	1.6387497731784606E-4
1104	7	-0.023879357887302888
1104	8	-0.03207870557211834
1104	9	-0.011451121268940767
1104	10-11	0.02975830799702095
1104	12-13	-0.0160021529009029
1104	14-15	0.02452954895711912
1104	16-17	0.05097072563333427
1104	18-19	-0.00259508218175597
1104	20-21	-0.03835680447118506
1104	22-23	0.022281907126867395
1104	24-25	-0.02780570564435436
1104	26-27	0.02136653733521854
1104	28-29	0.056731546276203915
1104	30-31	-0.01754880585968266
1104	32-33	-0.03289250406944433
1104	34-35	0.020910094055174966
1104	36-37	-0.002763249527674816
1104	38-39	-0.022986902667334164
1104	40-41	-0.027544499608296746
1104	42-43	-0.0195154277017906
1104	44-45	-0.11182774658608707
1104	46-47	-0.11081426685399975
1104	48-49	-0.13134608391094815
1104	50-51	-0.1457885838871391
1104	52-53	-0.1448213382298249
1104	54-55	-0.1767068982353308
1104	56-57	-0.11633323270700657
1104	58-59	-0.1783866153757785
1104	60-61	-0.13449608771595223
1104	62-63	-0.19567857121982257
1104	64-65	-0.2815549598504745
1104	66-67	-0.15902875424174567
1104	68-69	-0.20507979177070723
1104	70-71	-0.1892608399993918
1104	72-73	-0.16639685557291273
1104	74-75	-0.20902598578986442
1104	76-77	-0.22737161900425207
1104	78-79	-0.2123990594504761
1104	80-81	-0.0995628363972898
1104	82-83	-0.13303104613456185
1104	84-85	-0.19649491658079654
1104	86-87	-0.15865998469742948
1104	88-89	-0.08749388432421767
1104	90-91	-0.1439707125965839
1104	92-93	-0.08406778252623326
1104	94-95	-0.03511700230802006
1104	96-97	-0.01827705409203162
1104	98-99	-0.03360588438690826
1104	100	0.03848049938968856
1105	1	0.0947088200805517
1105	2	0.09697952885135308
1105	3	0.14701898596865348
1105	4	0.13306982733975303
1105	5	0.08843259880523391
1105	6	0.16391472783153915
1105	7	0.09484211210847349
1105	8	0.1253810275251226
1105	9	0.08835023476926551
1105	10-11	0.1204138939890953
1105	12-13	0.16193940729436207
1105	14-15	0.2169862056873626
1105	16-17	0.25764111169200987
1105	18-19	0.13668184973610664
1105	20-21	0.126825625107422
1105	22-23	0.20661210689781484
1105	24-25	0.19136102532481658
1105	26-27	0.18996033302383353
1105	28-29	0.18194161239271267
1105	30-31	0.21662836242064287
1105	32-33	0.19752121652739874
1105	34-35	0.1121368639307434
1105	36-37	0.17137369246199796
1105	38-39	0.13578272313272066
1105	40-41	0.04591142813464444
1105	42-43	0.07317566240834594
1105	44-45	0.02046669524113298
1105	46-47	0.12216337212063166
1105	48-49	0.08044822998814283
1105	50-51	0.09534046310918853
1105	52-53	0.038066801816960094
1105	54-55	0.0394332155597894
1105	56-57	0.10478130561457277
1105	58-59	0.10591639966693833
1105	60-61	0.03566147710096601
1105	62-63	0.13161179924380662
1105	64-65	0.12869185514514214
1105	66-67	0.12439158206710488
1105	68-69	0.10157663387664684
1105	70-71	0.043771320727081786
1105	72-73	0.10149112710195851
1105	74-75	-0.02223702761220636
1105	76-77	0.08910362081022782
1105	78-79	0.06169600130522923
1105	80-81	0.03500872619408568
1105	82-83	0.05854039360519181
1105	84-85	0.07188419336430485
1105	86-87	0.13826103389479982
1105	88-89	0.12583196284430898
1105	90-91	0.09492809523505485
1105	92-93	0.05705169524269138
1105	94-95	0.07917784970146613
1105	96-97	0.1260878385701787
1105	98-99	0.14763954675120772
1105	100	0.15642648122795677
1106	1	0.019644587149823423
1106	2	0.06474129259836303
1106	3	0.01952767973088143
1106	4	-0.02385044620326937
1106	5	-0.017675399844129913
1106	6	0.0028274641931957945
1106	7	-0.04582344584476772
1106	8	-0.0013504477752803723
1106	9	-0.012306212198780031
1106	10-11	-0.05723180900248792
1106	12-13	-0.058863839088409975
1106	14-15	-0.0037959127185729358
1106	16-17	-0.03960099600197964
1106	18-19	-0.07447056860462453
1106	20-21	-0.12574213966420444
1106	22-23	-0.08878337680913972
1106	24-25	-0.04415925171123547
1106	26-27	-0.026034782556621394
1106	28-29	-0.02193264242766446
1106	30-31	-0.01228096823728464
1106	32-33	-0.0029534716226962132
1106	34-35	-0.016974625807520738
1106	36-37	-0.09519932543784648
1106	38-39	-0.1311123326781285
1106	40-41	-0.1383249180012882
1106	42-43	-0.10702065555048534
1106	44-45	-0.11319456240472192
1106	46-47	-0.10940565790389911
1106	48-49	-0.08688335747731912
1106	50-51	-0.15058021213221906
1106	52-53	-0.13929705735962017
1106	54-55	-0.1584545204137484
1106	56-57	-0.12709831294660745
1106	58-59	-0.19107843064945484
1106	60-61	-0.19434320938023575
1106	62-63	-0.1593370336962323
1106	64-65	-0.17312575729159363
1106	66-67	-0.1227073841902353
1106	68-69	-0.17972139527850572
1106	70-71	-0.157759693493432
1106	72-73	-0.12827343786062073
1106	74-75	-0.07462837013867585
1106	76-77	-0.11742681609013772
1106	78-79	-0.0863644940839734
1106	80-81	-0.03717948211467359
1106	82-83	-0.12016199931404259
1106	84-85	-0.10152463468550366
1106	86-87	-0.11486107950446467
1106	88-89	-0.13181175591248717
1106	90-91	-0.04745712478698749
1106	92-93	-0.043381464668193814
1106	94-95	-0.04124492329819063
1106	96-97	-0.02381680657684271
1106	98-99	-0.0010374683386800143
1106	100	0.028826211876573637
1107	1	0.04507940379791009
1107	2	0.06471474390511744
1107	3	0.034787803172957155
1107	4	-0.005265426116473293
1107	5	-0.04287548491212334
1107	6	-0.01880032593190606
1107	7	0.07312456218527785
1107	8	-0.05385572362695257
1107	9	-0.08241925447804022
1107	10-11	-0.08154583143333127
1107	12-13	-0.049674696008246144
1107	14-15	-0.11225870598698862
1107	16-17	-0.08133402271660373
1107	18-19	-0.06491521247586718
1107	20-21	-0.0626828234332848
1107	22-23	-0.12199268380879147
1107	24-25	-0.07544052997472761
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2216	6	-0.027685801268994226
2216	7	0.012683860876947506
2216	8	0.004876671568176505
2216	9	-0.07074396565036523
2216	10-11	-0.05924239021998545
2216	12-13	-0.0832021898138322
2216	14-15	-0.1746225906089336
2216	16-17	-0.11295475356980944
2216	18-19	-0.184537191041116
2216	20-21	-0.15758415412994253
2216	22-23	-0.13972367238516625
2216	24-25	-0.17246144092222693
2216	26-27	-0.224176286662356
2216	28-29	-0.1772714646005369
2216	30-31	-0.19889429539727388
2216	32-33	-0.08961032310953243
2216	34-35	-0.08057546046261876
2216	36-37	-0.06024833134753038
2216	38-39	-0.04937024849655103
2216	40-41	-0.07963135602882687
2216	42-43	-0.0814092103338595
2216	44-45	-0.07619392782035561
2216	46-47	0.054145446559594745
2216	48-49	0.07317965445447783
2216	50-51	0.04624899941644145
2216	52-53	0.059211722907996034
2216	54-55	0.04328430340872558
2216	56-57	-0.04731286059597295
2216	58-59	-0.00614116282165611
2216	60-61	0.02049516392818873
2216	62-63	0.08875038025092152
2216	64-65	0.06254901510683197
2216	66-67	0.02377767151246246
2216	68-69	0.07459991886113926
2216	70-71	0.021318187174813374
2216	72-73	0.06482967732090117
2216	74-75	0.09032896741607033
2216	76-77	0.06162132401220077
2216	78-79	0.07598342604524078
2216	80-81	0.11958099858963323
2216	82-83	0.1055526575625052
2216	84-85	0.07129180602622398
2216	86-87	0.0344155050223236
2216	88-89	0.06390553518512121
2216	90-91	0.06676617792982142
2216	92-93	0.1233889569193991
2216	94-95	0.07005656708880537
2216	96-97	0.12212827941126392
2216	98-99	0.15267797495774005
2216	100	0.1572378067160045
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	26578.0
3	4827.0
4	3108.0
5	2611.0
6	2158.0
7	2519.0
8	3007.0
9	3367.0
10	3623.0
11	4084.0
12	4455.0
13	5239.0
14	5880.0
15	6126.0
16	6344.0
17	6352.0
18	6248.0
19	5949.0
20	5784.0
21	6195.0
22	7033.0
23	9813.0
24	15458.0
25	21493.0
26	22385.0
27	18558.0
28	19269.0
29	24518.0
30	29988.0
31	36425.0
32	48210.0
33	65903.0
34	91509.0
35	137194.0
36	252787.0
37	559103.0
38	1029617.0
39	531210.0
40	1461.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.54722981735877	20.354642854192655	13.436182955136836	23.661944373311748
2	14.853574144060437	20.498037170122256	41.46022277278323	23.188165913034073
3	17.8064797632218	25.962554059269028	30.353536002657528	25.877430174851646
4	11.574862973169232	16.996532651197363	40.500538354992734	30.928066020640678
5	12.130618318115683	38.58712412753305	36.6024265651688	12.679830989182465
6	29.91612243634251	39.34686432426949	18.321941495843998	12.415071743544004
7	26.23535785862851	33.211941960058425	23.341199564585164	17.2115006167279
8	23.92798911700863	37.595794369435396	21.013824429417017	17.462392084138962
9	24.27763886340631	17.401920982079773	21.788313822552745	36.53212633196117
10-11	22.838596340394652	27.622613677707875	29.899223078733545	19.63956690316393
12-13	23.66551072855064	24.96775275221531	29.797543938933792	21.56919258030026
14-15	20.92518279582289	27.198914959899795	26.709708127836436	25.166194116440877
16-17	20.596277064704616	30.575776725996924	27.283798177319447	21.544148031979013
18-19	20.46454128253001	29.599691944033424	29.266568549040585	20.669198224395977
20-21	21.725933757477943	28.863777900064186	28.654220135630304	20.756068206827567
22-23	21.202518511686737	28.66702679912514	28.792688871907036	21.33776581728109
24-25	21.192596193012083	28.821379376342072	28.641204063242377	21.34482036740347
26-27	20.976280284561483	28.72212170053056	28.754188724068452	21.547409290839504
28-29	20.948239940576478	28.734927952276724	28.86372283078626	21.45310927636054
30-31	21.201980104743427	29.27061376593114	28.43673115380827	21.090674975517153
32-33	20.84945423640038	28.83422193892257	29.046861385763535	21.269462438913514
34-35	21.007333961473933	28.637331808931286	29.01247041900799	21.34286381058679
36-37	21.334613258290076	29.096702678142517	28.421991851100525	21.146692212466885
38-39	21.09492329281996	29.157275863732913	28.658114715990003	21.089686127457124
40-41	20.87738716589394	28.84899750683894	28.926119332809748	21.347495994457375
42-43	20.901631380097303	28.711841775464315	28.87057946254802	21.515947381890367
44-45	21.054485569599407	28.90195561776936	28.838848861624605	21.204709951006627
46-47	21.08077833325017	29.143395041326126	28.69078302833403	21.085043597089673
48-49	21.003790504866	28.933945619478628	28.746297678962122	21.315966196693246
50-51	20.975891719823892	28.84534026524954	28.78169157309431	21.397076441832258
52-53	21.019757773933755	28.820073434574855	28.777157552338934	21.38301123915246
54-55	21.20580470415269	29.16698503703196	28.60014306905519	21.027067189760153
56-57	21.02483724880871	28.873254396418808	28.61731844265393	21.484589912118558
58-59	20.860617707527975	28.52721603460783	29.058255792603347	21.55391046526085
60-61	21.166861862178354	28.99990514683796	28.565869592711064	21.267363398272618
62-63	20.998538680504574	28.97707305971715	28.657572035937196	21.36681622384108
64-65	20.9453936222628	28.728272983804036	28.82921781424307	21.4971155796901
66-67	20.905725450984637	28.763653208142664	28.663334682001633	21.667286658871067
68-69	21.270922528657913	28.940742000806573	28.58160311076954	21.20673235976597
70-71	21.243113765355393	28.724939573054275	28.632804742333708	21.39914191925662
72-73	20.94370739603583	28.834025371152112	28.786500775197627	21.435766457614434
74-75	21.025979661217907	28.990924038741007	28.604320859066945	21.37877544097414
76-77	21.06392312678179	28.941115947451344	28.55309076393683	21.441870161830035
78-79	21.211037848946738	29.017185503313264	28.58945815273954	21.18231849500046
80-81	21.161662212474912	28.804916940260917	28.650204518459027	21.383216328805148
82-83	20.9835102367744	28.849494188336628	28.821499663407007	21.345495911481972
84-85	21.017481358426885	28.76405312204049	28.662300704658954	21.55616481487367
86-87	21.21337922023986	28.900023400148633	28.4923898468056	21.394207532805908
88-89	21.030500644323812	28.78716872073354	28.58003091495307	21.602299719989578
90-91	21.063588748864717	28.65259473997596	28.6444270007896	21.639389510369718
92-93	21.1767052531236	28.89009238019914	28.466344893808	21.46685747286926
94-95	20.92903642598022	28.648776690023027	28.69419735294605	21.727989531050703
96-97	20.961283942600915	28.689140189795314	28.632042577227544	21.717533290376227
98-99	21.071398519714066	28.685476011112414	28.57883738387852	21.664288085295
100	21.178423236514522	28.65134461367721	28.405595448107935	21.764636701700336
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	493.0
1	465.0
2	410.5
3	335.0
4	269.5
5	231.5
6	205.5
7	208.0
8	202.5
9	205.0
10	225.0
11	216.0
12	228.5
13	277.0
14	327.0
15	411.0
16	560.0
17	800.0
18	1159.5
19	1693.0
20	2504.0
21	3761.0
22	5865.0
23	9049.5
24	12946.0
25	17796.5
26	24434.5
27	33072.5
28	43440.0
29	53823.0
30	62946.5
31	72242.5
32	82155.5
33	91475.5
34	99755.5
35	106389.5
36	111097.5
37	115338.0
38	117807.0
39	117374.0
40	116730.5
41	115137.5
42	111744.5
43	108043.0
44	104003.0
45	99952.5
46	96361.0
47	92650.0
48	89088.0
49	86283.5
50	82658.5
51	78398.0
52	73227.5
53	68737.5
54	66226.5
55	62972.0
56	57908.5
57	52123.5
58	46891.5
59	41932.0
60	37044.5
61	31686.0
62	26523.5
63	22220.5
64	18456.5
65	14687.0
66	11422.0
67	8676.5
68	6257.5
69	4567.0
70	3216.0
71	2277.0
72	1771.5
73	1354.0
74	972.0
75	714.5
76	452.5
77	326.0
78	258.0
79	148.0
80	84.0
81	57.0
82	40.0
83	31.0
84	24.0
85	17.5
86	12.0
87	8.5
88	8.5
89	9.0
90	6.5
91	5.0
92	5.5
93	4.5
94	4.5
95	4.5
96	4.5
97	3.5
98	2.5
99	1.5
100	2.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.32812012167088
2	0.11599308125312048
3	0.0654395946763062
4	0.04004758285173041
5	0.04334096959940561
6	0.009748424773118587
7	0.0066855750977806525
8	0.015083711304352408
9	0.01794895777482983
10-11	0.020534266371754862
12-13	0.015478917714073432
14-15	0.018294763383335726
16-17	0.01924984554016153
18-19	0.018969907666609144
20-21	0.023366578974755532
22-23	0.015413049979119927
24-25	0.02415699179419758
26-27	0.01841003191950436
28-29	0.018212428714643847
30-31	0.019398047943806916
32-33	0.024140524860459204
34-35	0.014606170225939505
36-37	0.008974478887414915
38-39	0.012992410719578658
40-41	0.009303817562182435
42-43	0.00813466526675774
44-45	0.008661607146385772
46-47	0.007690058055821588
48-49	0.003853262494779982
50-51	0.006273901754321252
52-53	0.007113715374978429
54-55	0.0030299158078611827
56-57	0.0026841101993552866
58-59	0.0026511763318785345
60-61	0.0038203286273032298
62-63	0.00299698194038443
64-65	0.0025029739282331504
66-67	0.001959565114866743
68-69	0.0032110520789833184
70-71	0.002535907795709903
72-73	0.0021571683197272548
74-75	0.0023218376571110147
76-77	0.0038203286273032298
78-79	0.0035568576874892145
80-81	0.003606258488704342
82-83	0.0026347093981401587
84-85	0.0031781182115065663
86-87	0.0027170440668320387
88-89	0.5963500053352866
90-91	0.6065430373193413
92-93	0.603249650571666
94-95	0.5907677147979771
96-97	0.5886270134119882
98-99	0.5856300314716038
100	0.5882976747372206
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3036388.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.09875075721288
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.7261907865544	52.153303028945885
2	13.87352836052019	18.617928431713366
3	4.132915506137745	8.319404023408705
4	1.7207047211350874	4.618285488408109
5	0.8378758914797018	2.8110212803937027
6	0.4816489759952027	1.939082675566135
7	0.2874996767520711	1.3503608407216543
8	0.1931205241311294	1.0366516731821571
9	0.14069292191254681	0.8496287370642593
>10	0.5744063933637957	6.328235949178125
>50	0.023678557780623076	1.0751367935170482
>100	0.007639482027347063	0.8526619075245498
>500	9.820221007325068E-5	0.04829917037634168
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	3.293386747675198E-5	0.0
6	0.0	0.0	0.0	3.293386747675198E-5	0.0
7	0.0	0.0	0.0	3.293386747675198E-5	0.0
8	0.0	0.0	0.0	3.293386747675198E-5	0.0
9	0.0	0.0	0.0	6.586773495350396E-5	0.0
10-11	0.0	0.0	0.0	9.880160243025595E-5	0.0
12-13	0.0	1.646693373837599E-5	0.0	9.880160243025595E-5	0.0
14-15	0.0	3.293386747675198E-5	0.0	1.1526853616863195E-4	0.0
16-17	0.0	3.293386747675198E-5	0.0	1.3173546990700793E-4	0.0
18-19	0.0	3.293386747675198E-5	0.0	1.976032048605119E-4	0.0
20-21	0.0	3.293386747675198E-5	0.0	2.3053707233726387E-4	0.0
22-23	0.0	4.940080121512797E-5	0.0	3.622725422442718E-4	0.0
24-25	0.0	9.880160243025595E-5	0.0	5.434088133664076E-4	0.0
26-27	0.0	9.880160243025595E-5	0.0	7.245450844885436E-4	0.0
28-29	0.0	9.880160243025595E-5	0.0	0.001597292572622471	0.0
30-31	0.0	9.880160243025595E-5	0.0	0.005812827609646725	0.0
32-33	0.0	9.880160243025595E-5	0.0	0.014507368623509247	0.0
34-35	0.0	9.880160243025595E-5	0.0	0.026314160113924834	0.0
36-37	0.0	1.1526853616863195E-4	0.0	0.046864893419418074	0.0
38-39	0.0	1.3173546990700793E-4	0.0	0.08557865463834002	0.0
40-41	0.0	1.3173546990700793E-4	0.0	0.1314884659009323	0.0
42-43	0.0	1.3173546990700793E-4	0.0	0.17583391845837884	0.0
44-45	0.0	1.3173546990700793E-4	0.0	0.22660147517379203	0.0
46-47	0.0	1.3173546990700793E-4	0.0	0.2915799957054237	0.0
48-49	0.0	1.3173546990700793E-4	0.0	0.3653847927208249	0.0
50-51	0.0	1.3173546990700793E-4	0.0	0.44414613679147724	0.0
52-53	0.0	1.3173546990700793E-4	0.0	0.5239284307539089	0.0
54-55	0.0	1.3173546990700793E-4	0.0	0.60990229180197	0.0
56-57	0.0	1.3173546990700793E-4	0.0	0.7106634593470926	0.0
58-59	0.0	1.3173546990700793E-4	0.0	0.826607139798998	0.0
60-61	0.0	1.3173546990700793E-4	0.0	0.953880070662906	0.0
62-63	0.0	1.3173546990700793E-4	0.0	1.0801155847012964	0.0
64-65	0.0	1.3173546990700793E-4	0.0	1.2134483471809268	0.0
66-67	0.0	1.3173546990700793E-4	0.0	1.3655698810560442	0.0
68-69	0.0	1.3173546990700793E-4	0.0	1.538555019977684	0.0
70-71	0.0	1.3173546990700793E-4	0.0	1.7263768661976004	0.0
72-73	0.0	1.3173546990700793E-4	0.0	1.9238483355882054	0.0
74-75	0.0	1.3173546990700793E-4	0.0	2.1250413320036836	0.0
76-77	0.0	1.3173546990700793E-4	0.0	2.338963268198926	0.0
78-79	0.0	1.4820240364538394E-4	0.0	2.5818670077737105	0.0
80-81	0.0	1.6466933738375992E-4	0.0	2.84570022012997	0.0
82-83	0.0	1.6466933738375992E-4	0.0	3.1251111518027344	0.0
84-85	0.0	1.6466933738375992E-4	0.0	3.397787107576502	0.0
86-87	0.0	1.6466933738375992E-4	0.0	3.6838671474133085	0.0
88	0.0	1.6466933738375992E-4	0.0	3.922818822890882	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCAACGC	3710	0.0	34.943317	4
GTATCAA	3915	0.0	34.905415	1
ATCAACG	3795	0.0	34.291214	3
CAACGCA	3945	0.0	33.098816	5
GGTATCA	2405	0.0	31.349558	1
AACGCAG	4550	0.0	28.793407	6
GTACATG	7885	0.0	27.610027	1
TATCAAC	4845	0.0	27.554792	2
TACATGG	7765	0.0	26.697449	2
ACATGGG	7995	0.0	25.091364	3
CGCAGAG	5020	0.0	24.881592	8
ACGCAGA	5190	0.0	24.609442	7
CATGGGG	4810	0.0	20.800007	4
GAGTACA	5475	0.0	18.59072	1
GCAGAGT	6475	0.0	18.420193	9
CCGATCG	130	0.008084436	18.060379	9
CTGTCGC	730	0.0	18.010899	9
TCTGTCG	710	0.0	17.856882	8
GTATAGA	1335	0.0	17.648804	1
AGAGTAC	5035	0.0	16.180809	10-11
>>END_MODULE