FastQCFastQC Report
Wed 25 May 2016
SRR1033125_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033125_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3036388
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58210.1917080425821733No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46230.15225326934502442No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44950.047.2114261
GTATCAA84000.042.2369271
TCAACGC96550.036.370854
ATCAACG96500.036.3409843
CAACGCA98700.035.483325
TATCAAC104900.033.610742
AACGCAG104550.033.5334556
ACGCAGA119400.029.2447577
CGCAGAG118850.029.14148
GTACATG73450.026.0737131
GCAGAGT135100.025.4275289
TACATGG75000.024.3199522
ACATGGG76250.022.7494893
GAGTACT93600.021.51397312-13
AGAGTAC118400.021.3338310-11
CAGAGTA132050.020.32075110-11
GTGGTAT24900.020.2201651
TACACCG2601.6816557E-819.8886135
CATGGGG46000.019.723534
AGTACTT100200.018.97127212-13
Produced by FastQC (version 0.11.5)