FastQCFastQC Report
Wed 25 May 2016
SRR1033124_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033124_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1775925
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA25800.032.5133671
GGTATCA18100.032.0249331
TCAACGC26800.029.97444
ATCAACG27850.029.0203423
GTACATG61650.028.8948461
TACATGG58950.028.6346242
CAACGCA28450.028.2351935
AACGCAG29400.027.4725536
ACATGGG60600.026.6738113
TATCAAC34850.023.7460162
ACGCAGA35650.022.261027
CGCAGAG36050.022.0140178
CATGGGG43250.021.6150444
GTCTAGG3700.020.3788621
TACACCG2801.8117134E-920.1326125
CTACGCT1708.662691E-519.3436434
TACTTCG2302.297469E-618.375229
TATACCG1300.0080642118.067735
GCAGAGT43000.017.9099049
GAGTACA42450.017.7624971
Produced by FastQC (version 0.11.5)