##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033124_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1775925
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.541468812027535	33.0	31.0	34.0	30.0	34.0
2	31.669951152216452	34.0	31.0	34.0	30.0	34.0
3	31.844676436223377	34.0	31.0	34.0	30.0	34.0
4	35.17851035375931	37.0	35.0	37.0	33.0	37.0
5	35.29788476427777	37.0	35.0	37.0	33.0	37.0
6	35.35042639751116	37.0	36.0	37.0	33.0	37.0
7	35.30606416374565	37.0	37.0	37.0	33.0	37.0
8	35.28374846910765	37.0	36.0	37.0	33.0	37.0
9	36.95020003660064	39.0	38.0	39.0	34.0	39.0
10-11	36.9442175204471	39.0	38.0	39.0	34.0	39.0
12-13	36.8874808902403	39.0	38.0	39.0	34.0	39.0
14-15	38.34387150358264	41.0	39.0	41.0	34.0	41.0
16-17	38.35851119838957	41.0	39.0	41.0	34.0	41.0
18-19	38.34659965933246	41.0	39.0	41.0	34.0	41.0
20-21	38.37461463744246	41.0	39.0	41.0	34.0	41.0
22-23	38.34243929219984	41.0	39.0	41.0	34.0	41.0
24-25	38.33627152047525	41.0	39.0	41.0	34.0	41.0
26-27	38.29748975885806	40.5	39.0	41.0	34.0	41.0
28-29	38.262432253614314	40.5	39.0	41.0	33.5	41.0
30-31	38.25730337711333	40.5	39.0	41.0	34.0	41.0
32-33	38.324621253712856	41.0	39.0	41.0	34.0	41.0
34-35	38.29326351056492	41.0	39.0	41.0	34.0	41.0
36-37	38.214267212860904	41.0	39.0	41.0	33.0	41.0
38-39	38.13233385418866	41.0	38.0	41.0	33.0	41.0
40-41	38.067789180286326	41.0	38.0	41.0	33.0	41.0
42-43	37.9475357912074	40.5	38.0	41.0	33.0	41.0
44-45	37.821368019482804	40.0	38.0	41.0	33.0	41.0
46-47	37.69160690907555	40.0	38.0	41.0	32.5	41.0
48-49	37.567051818066645	40.0	38.0	41.0	32.0	41.0
50-51	37.43336064304518	40.0	37.0	41.0	32.0	41.0
52-53	37.27905514027901	40.0	37.0	41.0	31.5	41.0
54-55	37.10914087025071	40.0	36.5	41.0	31.0	41.0
56-57	36.93638441938708	40.0	36.0	41.0	31.0	41.0
58-59	36.730819995213764	39.5	35.5	41.0	31.0	41.0
60-61	36.495462927770035	39.0	35.0	41.0	31.0	41.0
62-63	36.21953179329082	39.0	35.0	41.0	30.5	41.0
64-65	35.93435449132143	38.5	35.0	41.0	30.0	41.0
66-67	35.63552205188846	37.5	35.0	40.0	30.0	41.0
68-69	35.3139245745175	37.0	35.0	40.0	30.0	41.0
70-71	34.962750960766925	36.5	35.0	39.5	29.0	41.0
72-73	34.59385024705435	36.0	34.0	39.0	29.0	41.0
74-75	34.22634345481932	35.5	34.0	39.0	29.0	40.5
76-77	33.909432267691486	35.0	34.0	37.5	29.0	40.0
78-79	33.554913355012175	35.0	34.0	37.0	28.0	39.0
80-81	33.21477736953982	35.0	34.0	37.0	28.0	39.0
82-83	33.014346889649055	35.0	34.0	36.0	28.0	38.5
84-85	32.76390191027211	35.0	34.0	36.0	28.0	37.0
86-87	32.525318636766755	35.0	34.0	36.0	27.0	37.0
88-89	32.18336247307741	35.0	34.0	35.0	26.5	36.5
90-91	32.00824640680209	35.0	34.0	35.0	26.0	36.0
92-93	31.84272759266298	35.0	33.0	35.0	26.0	36.0
94-95	31.677595056097523	35.0	33.0	35.0	25.5	36.0
96-97	31.51016878528091	35.0	33.0	35.0	25.0	36.0
98-99	31.323752410715542	35.0	33.0	35.0	25.0	35.0
100	31.16341399552346	35.0	33.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.02211073827236021
1101	2	0.06798757116422038
1101	3	0.07196118915672045
1101	4	-0.01601897877115732
1101	5	-0.08893222313639626
1101	6	-0.0019980554183121058
1101	7	-0.046482895459192264
1101	8	-0.13603942245820377
1101	9	-0.06832268751919202
1101	10-11	-0.027306180209791364
1101	12-13	-0.05881700223184794
1101	14-15	0.0024383002779231333
1101	16-17	-0.09399088372586561
1101	18-19	-0.0860593357736974
1101	20-21	-0.07240618476819805
1101	22-23	-0.08394938946870667
1101	24-25	-0.04185317628642338
1101	26-27	0.07635030031974566
1101	28-29	0.032055704437958354
1101	30-31	0.020229456618992003
1101	32-33	0.07975669074539127
1101	34-35	0.07714869511764277
1101	36-37	-0.04160483700739803
1101	38-39	-0.050089327028175035
1101	40-41	-0.02212061912655372
1101	42-43	-0.04091837500796203
1101	44-45	-0.05100987580063787
1101	46-47	0.07250262468076585
1101	48-49	0.04938389145571165
1101	50-51	-0.1045726679606176
1101	52-53	-0.10705492059516786
1101	54-55	-0.1090128442380589
1101	56-57	-0.09627950282630593
1101	58-59	-0.04971589324873804
1101	60-61	-0.12504825707141265
1101	62-63	-0.0717197821920692
1101	64-65	-0.10152008471679608
1101	66-67	-0.10864142398746424
1101	68-69	-0.13512539571529913
1101	70-71	-0.10587255758360214
1101	72-73	0.03538043376485689
1101	74-75	-0.009740854369496788
1101	76-77	-0.01900070183646818
1101	78-79	-0.11213777501196631
1101	80-81	-0.10936273614139225
1101	82-83	-0.07045341966496466
1101	84-85	-0.0940885403942957
1101	86-87	-0.04239461504599973
1101	88-89	0.12238627928145007
1101	90-91	0.16200153533893058
1101	92-93	0.13901814932506085
1101	94-95	0.17774478836462393
1101	96-97	0.1330154516954174
1101	98-99	0.22271235078133955
1101	100	0.3188933622564143
1102	1	0.18856772690227075
1102	2	0.20129356402126675
1102	3	0.239595090663002
1102	4	0.23249007245551212
1102	5	0.15055238589692976
1102	6	0.09069161231951739
1102	7	0.05882963601656854
1102	8	0.061279852930425704
1102	9	0.0972406300086277
1102	10-11	0.12473001699833475
1102	12-13	0.09347501073504816
1102	14-15	0.1499695739549125
1102	16-17	0.11395669441261447
1102	18-19	0.13305728670126626
1102	20-21	0.060415362007759654
1102	22-23	0.07518124433207873
1102	24-25	0.09389565658424459
1102	26-27	0.17324408754133458
1102	28-29	0.16305446369143084
1102	30-31	0.15142701438844597
1102	32-33	0.10187897998047646
1102	34-35	0.16932111782600145
1102	36-37	0.14349173785215186
1102	38-39	0.06737918437751489
1102	40-41	0.12964949761524736
1102	42-43	0.13732386118424245
1102	44-45	0.11539867624046707
1102	46-47	0.0701451100487418
1102	48-49	0.1567179287769065
1102	50-51	0.16267468711293986
1102	52-53	0.12095358499814068
1102	54-55	0.13523643354353254
1102	56-57	0.06182770755336264
1102	58-59	0.04335140345712318
1102	60-61	0.07087622098424617
1102	62-63	0.14630404989270573
1102	64-65	0.18760809838505565
1102	66-67	0.12232002946946352
1102	68-69	0.025199151883761317
1102	70-71	0.0036555609789061805
1102	72-73	0.03652222209503719
1102	74-75	0.14177055966454333
1102	76-77	0.06131606383116406
1102	78-79	0.17257618287322174
1102	80-81	0.08960627021566836
1102	82-83	0.020413540743412284
1102	84-85	0.0582559990058229
1102	86-87	0.07243185686495224
1102	88-89	0.21098890935615344
1102	90-91	0.2797263436615509
1102	92-93	0.24545338319038024
1102	94-95	0.21662629771288522
1102	96-97	0.12775815717721883
1102	98-99	0.06953187925916637
1102	100	0.11954375978929477
1103	1	0.11225525454008078
1103	2	0.03321592680520169
1103	3	0.15167333536511762
1103	4	0.19786944874072532
1103	5	0.08901604494631954
1103	6	0.14626161697857043
1103	7	0.15078378267018877
1103	8	4.1040567412409246E-4
1103	9	0.15834536896114315
1103	10-11	0.11159749709754863
1103	12-13	0.1739085961064788
1103	14-15	0.0805632013389399
1103	16-17	0.12928698532608252
1103	18-19	0.15190720770937105
1103	20-21	0.1342973444330724
1103	22-23	0.1364569556801527
1103	24-25	0.15589977426689217
1103	26-27	0.18495230525584816
1103	28-29	0.11158397634915929
1103	30-31	0.16652138990339438
1103	32-33	0.1433666440843453
1103	34-35	0.10695540872949039
1103	36-37	0.1559422392776284
1103	38-39	0.18037985361009135
1103	40-41	0.2231293760431896
1103	42-43	0.17062839506343863
1103	44-45	0.07336862501580299
1103	46-47	0.25752571003789626
1103	48-49	0.2626489323326311
1103	50-51	0.22916283458610565
1103	52-53	0.185065972412076
1103	54-55	0.2866837503450057
1103	56-57	0.264630672509405
1103	58-59	0.25641816954193786
1103	60-61	0.2732751827693818
1103	62-63	0.3047370664476361
1103	64-65	0.3110109526963001
1103	66-67	0.32686951681735366
1103	68-69	0.28334774459305123
1103	70-71	0.2131676137141838
1103	72-73	0.18650781457169074
1103	74-75	0.32859637644948947
1103	76-77	0.20904580301757392
1103	78-79	0.18605267459763297
1103	80-81	0.2209185274005705
1103	82-83	0.10413770153636648
1103	84-85	0.17182275604695008
1103	86-87	0.236866604817493
1103	88-89	0.31448069114266985
1103	90-91	0.3030420511676226
1103	92-93	0.25218048037380925
1103	94-95	0.20460139515077813
1103	96-97	0.23187512256610532
1103	98-99	0.28899128216399816
1103	100	0.37665505759529694
1104	1	0.12091999840394152
1104	2	0.093053004144938
1104	3	0.1368190771454394
1104	4	0.14971597632263212
1104	5	0.09796511493452442
1104	6	0.18717500896649142
1104	7	0.17692495400283548
1104	8	0.10324001926355209
1104	9	0.15175127748547368
1104	10-11	0.1336308762263485
1104	12-13	0.15993036563097007
1104	14-15	0.02660833867982859
1104	16-17	0.0914226308752788
1104	18-19	0.054479866302692415
1104	20-21	0.03601634227067052
1104	22-23	0.00658201368268152
1104	24-25	0.015969847387992786
1104	26-27	0.013156546946703429
1104	28-29	0.06557462618977894
1104	30-31	0.0833592588070644
1104	32-33	0.06510773595731933
1104	34-35	0.022795179206276828
1104	36-37	-0.01051379859612922
1104	38-39	-0.02711642361318667
1104	40-41	0.013135123221914569
1104	42-43	0.10441225261832443
1104	44-45	-0.08670235794890147
1104	46-47	-0.1039851762407551
1104	48-49	-0.1374490274947462
1104	50-51	-0.15581519269100852
1104	52-53	-0.16322769174039564
1104	54-55	-0.15491240344282176
1104	56-57	-0.05085099869131682
1104	58-59	-0.16144805008824648
1104	60-61	-0.09489636520818578
1104	62-63	-0.21305158901581933
1104	64-65	-0.26074894261103054
1104	66-67	-0.1389613826843643
1104	68-69	-0.12428644975560843
1104	70-71	-0.07864697835744039
1104	72-73	-0.060227211677293724
1104	74-75	-0.04859907615220749
1104	76-77	-0.1791871897309747
1104	78-79	-0.19761297910983444
1104	80-81	-0.21259063320805183
1104	82-83	-0.23608519498105807
1104	84-85	-0.1553062021517988
1104	86-87	-0.21835271298177616
1104	88-89	-0.05542570111516909
1104	90-91	-0.12784403034829595
1104	92-93	-0.16497029890723525
1104	94-95	-0.18944731787545166
1104	96-97	-0.11636266202538792
1104	98-99	-0.0032742132306786687
1104	100	-0.03971181560586601
1105	1	0.2581573554575307
1105	2	0.263575385636571
1105	3	0.2431673940596184
1105	4	0.3280315409792962
1105	5	0.302902890339233
1105	6	0.31507280924930114
1105	7	0.3113497358793751
1105	8	0.23353263034956484
1105	9	0.30164624964429265
1105	10-11	0.3323549933177219
1105	12-13	0.319871925558445
1105	14-15	0.3300119154939125
1105	16-17	0.39132816449298957
1105	18-19	0.33390101034338926
1105	20-21	0.390231244489172
1105	22-23	0.33995930419907694
1105	24-25	0.36599434410513965
1105	26-27	0.3179608372460052
1105	28-29	0.3053304511277375
1105	30-31	0.34397000450473314
1105	32-33	0.2956701043058061
1105	34-35	0.3070330819352378
1105	36-37	0.41400638845903615
1105	38-39	0.31024664413857295
1105	40-41	0.241937835169864
1105	42-43	0.2945889223556364
1105	44-45	0.33513213812282316
1105	46-47	0.4026139753220406
1105	48-49	0.3589515686409399
1105	50-51	0.3847317148551497
1105	52-53	0.2895580397286466
1105	54-55	0.37173049016221427
1105	56-57	0.32406304276827314
1105	58-59	0.251967496379244
1105	60-61	0.28335964455529705
1105	62-63	0.3023825162819236
1105	64-65	0.3034722084706374
1105	66-67	0.3996382558790401
1105	68-69	0.3622036840815426
1105	70-71	0.29027182162612775
1105	72-73	0.3148225151916435
1105	74-75	0.30531731816572716
1105	76-77	0.3081593023978044
1105	78-79	0.3102407857568892
1105	80-81	0.2285601844248859
1105	82-83	0.2483074624580226
1105	84-85	0.21274303771653535
1105	86-87	0.2919200660314303
1105	88-89	0.25086027385417964
1105	90-91	0.3460211995687956
1105	92-93	0.30075409982303825
1105	94-95	0.31431666872323305
1105	96-97	0.4041042718070713
1105	98-99	0.4115518747462765
1105	100	0.43547873071493015
1106	1	0.10554060544390254
1106	2	0.1890221555851106
1106	3	0.17672372042039086
1106	4	0.2244906297813145
1106	5	0.1381452518958426
1106	6	0.09642410631788323
1106	7	0.13237226653667733
1106	8	0.12514457276459012
1106	9	0.15749470365064155
1106	10-11	0.18476413156859905
1106	12-13	0.19390558242093903
1106	14-15	0.23368974678361099
1106	16-17	0.19179883966695144
1106	18-19	0.20759074269243172
1106	20-21	0.1868721401222757
1106	22-23	0.16564896818695019
1106	24-25	0.22087536034769073
1106	26-27	0.15695218786228793
1106	28-29	0.18571407032095522
1106	30-31	0.18751775464519937
1106	32-33	0.1517843759443096
1106	34-35	0.1996952299846413
1106	36-37	0.23075260267641085
1106	38-39	0.26578673839225075
1106	40-41	0.1908820645967495
1106	42-43	0.18801490768171192
1106	44-45	0.27827887369303994
1106	46-47	0.31711674239602416
1106	48-49	0.2502236119423671
1106	50-51	0.26190687850531447
1106	52-53	0.26828245972502174
1106	54-55	0.3151291310177342
1106	56-57	0.36508758753792847
1106	58-59	0.2718192853940522
1106	60-61	0.29877148815735666
1106	62-63	0.3550480936895184
1106	64-65	0.22520501740030596
1106	66-67	0.20560021203693424
1106	68-69	0.2814144740740332
1106	70-71	0.31507880723440707
1106	72-73	0.23913539356157543
1106	74-75	0.28748168950702535
1106	76-77	0.22648203268669675
1106	78-79	0.2517013025165369
1106	80-81	0.2660565721581776
1106	82-83	0.25868063538552377
1106	84-85	0.19451256676735085
1106	86-87	0.1678470732440971
1106	88-89	0.3527766184182255
1106	90-91	0.36599544548428753
1106	92-93	0.34914208961180293
1106	94-95	0.31704165255807126
1106	96-97	0.31134465258447364
1106	98-99	0.35450630919737236
1106	100	0.4333776132356846
1107	1	-0.032236164423657954
1107	2	-0.022501474950388456
1107	3	-0.10573775717062972
1107	4	-0.09810001579322147
1107	5	-0.17122742853683803
1107	6	-0.13461746434936117
1107	7	-0.09384942921329298
1107	8	-0.04759488700089065
1107	9	-0.11438170618388455
1107	10-11	-0.17497959921644224
1107	12-13	-0.18376621735019683
1107	14-15	-0.21469251710605164
1107	16-17	-0.1996506370909401
1107	18-19	-0.1211941277173878
1107	20-21	-0.1382967183470285
1107	22-23	-0.20373933753473494
1107	24-25	-0.1854682013979243
1107	26-27	-0.08653332114979406
1107	28-29	-0.10692817167882396
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2216	10-11	-0.15354484346988073
2216	12-13	-0.18917749559079766
2216	14-15	-0.1781261046962186
2216	16-17	-0.2682650592285256
2216	18-19	-0.20997096671993631
2216	20-21	-0.2172193472051802
2216	22-23	-0.14387987724924045
2216	24-25	-0.1457832286142846
2216	26-27	-0.23644313122593985
2216	28-29	-0.19918135410809867
2216	30-31	-0.19673405150122392
2216	32-33	-0.13858861797427124
2216	34-35	-0.11742931499495057
2216	36-37	-0.18133911895171195
2216	38-39	-0.20831110953716347
2216	40-41	-0.19265556144614493
2216	42-43	-0.2532735830534918
2216	44-45	-0.1712455691579038
2216	46-47	-0.10409457791661225
2216	48-49	-0.15507079205056584
2216	50-51	-0.1320095352016537
2216	52-53	-0.10583424799149554
2216	54-55	-0.18261531166315592
2216	56-57	-0.23922498562198768
2216	58-59	-0.1918673577669594
2216	60-61	-0.11596698080111878
2216	62-63	-0.03054113943871073
2216	64-65	0.023796721550354505
2216	66-67	-0.03353864087232239
2216	68-69	-0.08433153502023316
2216	70-71	-0.14323468612250423
2216	72-73	-0.002719705643031034
2216	74-75	-0.07773548973960231
2216	76-77	-0.10883553447871464
2216	78-79	-0.020096985860355687
2216	80-81	-0.01889987200787857
2216	82-83	0.030682696853553182
2216	84-85	-0.01830513266456535
2216	86-87	-0.07812576582818309
2216	88-89	-0.041490478681446064
2216	90-91	-0.035222020497240436
2216	92-93	-0.027151169803445896
2216	94-95	0.03938738257000196
2216	96-97	-0.018130750096190695
2216	98-99	-0.026459028474302926
2216	100	-0.021026696196145167
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	20175.0
3	3682.0
4	2417.0
5	1987.0
6	1653.0
7	1704.0
8	2021.0
9	2200.0
10	2348.0
11	2526.0
12	2842.0
13	3131.0
14	3358.0
15	3720.0
16	3866.0
17	3794.0
18	3651.0
19	3510.0
20	3588.0
21	3756.0
22	4382.0
23	5940.0
24	9575.0
25	12978.0
26	13290.0
27	11458.0
28	12109.0
29	15354.0
30	18742.0
31	22826.0
32	29860.0
33	41260.0
34	58303.0
35	90773.0
36	175814.0
37	369896.0
38	569890.0
39	237009.0
40	537.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.73569553257111	19.164186896076753	13.166748493917193	23.933369077434943
2	15.469735980872413	19.726255839952465	40.468368838628166	24.335639340546958
3	18.697478754849957	25.031807153425987	29.302924680435304	26.967789411288752
4	12.279323126147744	16.400671466071813	38.034722456934915	33.28528295084553
5	13.30404044671652	38.11781936992127	35.03536271071866	13.542777472643541
6	31.6143891698296	37.46505760848754	17.61569563797317	13.304857583709694
7	28.26278294627336	32.335711385650484	21.727728495463793	17.67377717261236
8	25.572892878844794	36.919243940775395	19.668904493543604	17.838958686836207
9	25.25687819707408	17.28902197339899	20.34965283077098	37.10444699875595
10-11	24.454654483329385	26.99794350568218	28.12162349004798	20.42577852094045
12-13	24.788461202728644	24.1341481202402	28.49363539243298	22.583755284598173
14-15	22.012910855960182	26.144722670026958	25.271993861359654	26.570372612653202
16-17	22.177408568084076	29.582859439031168	25.8546781709759	22.385053821908855
18-19	22.161213884144857	28.59045208088994	27.519503723342098	21.728830311623106
20-21	23.13665956184392	27.74192512817827	27.24337664656309	21.878038663414724
22-23	22.78956138982484	27.488248144228933	27.265319086748324	22.456871379197906
24-25	22.61929335406331	27.49417990192779	27.312262236286266	22.57426450772263
26-27	22.4535471331937	27.69212054176098	27.224339483120513	22.629992841924807
28-29	22.31984059670358	27.74740344510262	27.306684643465783	22.626071314728023
30-31	22.45132084482706	27.991429404946818	27.12741452777249	22.429835222453633
32-33	22.42098860343502	27.720715164724997	27.376340089043584	22.481956142796395
34-35	22.514328257175954	27.554152280216055	27.44380062477227	22.487718837835722
36-37	22.695266107247058	27.69952060206029	27.130246107461048	22.474967183231602
38-39	22.54648265826292	27.900097763381083	27.11286602136828	22.440553556987716
40-41	22.319685364835895	27.732179430793725	27.408798769661182	22.539336434709206
42-43	22.426256723067887	27.59536746764396	27.377605109616066	22.60077069967209
44-45	22.356582660342305	27.80576862323521	27.3877267385187	22.44992197790378
46-47	22.451371158206058	27.770247516891	27.358066626947437	22.420314697955508
48-49	22.5288683830355	27.737523449886154	27.179850455680242	22.553757711398102
50-51	22.353131974768846	27.816775400441657	27.250164079083444	22.579928545706053
52-53	22.388018517778114	27.760787067513533	27.343005180180054	22.508189234528302
54-55	22.454804224372637	27.940773191957813	27.284846710231804	22.31957587343775
56-57	22.33233280298937	27.813748756610412	27.21965277721077	22.634265663189446
58-59	22.261639000311675	27.499087079264605	27.490049375145524	22.749224545278192
60-61	22.494019150543405	27.771525778960417	27.251022241693047	22.483432828803128
62-63	22.239387348579232	27.852608906814197	27.27543270618212	22.63257103842445
64-65	22.350752382888363	27.680189831470514	27.27551972502817	22.693538060612955
66-67	22.260762919994672	27.709153750449424	27.248683132866958	22.781400196688946
68-69	22.4624895016216	27.773324560166163	27.20703858025604	22.5571473579562
70-71	22.422720177669262	27.657718634775698	27.247303314856154	22.672257872698893
72-73	22.276726790307542	27.75807254478866	27.318970255014936	22.64623040988887
74-75	22.322043957727065	27.866594064490553	27.17189935401332	22.639462623769067
76-77	22.378621396060183	27.70742098657052	27.211888940006272	22.702068677363027
78-79	22.534582335817845	27.7725487303269	27.2087407274348	22.484128206420458
80-81	22.393252875003345	27.742525835964678	27.317606747124994	22.546614541906983
82-83	22.30577690084313	27.709286028415157	27.40909733355182	22.57583973718989
84-85	22.302642785447716	27.667667583117595	27.238470104131913	22.79121952730278
86-87	22.42564539761088	27.780364911632642	27.236972952031724	22.55701673872476
88-89	22.330603916130187	27.67050076704668	27.2561312748837	22.742764041939438
90-91	22.294890542378816	27.612419342319438	27.30208309136265	22.790607023939092
92-93	22.366376642451556	27.7333779015995	27.235752436500842	22.6644930194481
94-95	22.203003191485745	27.618672400842453	27.396333360146198	22.781991047525608
96-97	22.289158844570046	27.591360700687655	27.355430349140104	22.764050105602198
98-99	22.26950768395047	27.718242970929257	27.25874383744652	22.753505507673754
100	22.327670686598278	27.40938160272351	27.26640874151028	22.996538969167936
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	545.0
1	530.0
2	490.0
3	436.0
4	348.0
5	263.0
6	240.0
7	244.5
8	237.5
9	227.5
10	220.0
11	218.5
12	240.0
13	262.0
14	264.5
15	285.5
16	344.0
17	446.5
18	591.0
19	844.0
20	1258.0
21	1812.5
22	2547.0
23	3884.5
24	5589.5
25	7480.5
26	10143.5
27	13425.0
28	17220.5
29	21412.5
30	25494.5
31	29595.5
32	33513.0
33	37367.0
34	41849.5
35	45435.0
36	48161.5
37	52065.5
38	55137.5
39	56809.0
40	59081.5
41	61458.5
42	62253.0
43	62866.5
44	63024.0
45	61879.0
46	61561.0
47	60500.0
48	59680.5
49	59439.0
50	57917.5
51	55938.5
52	52991.0
53	50606.5
54	49612.5
55	47578.0
56	44078.0
57	40242.0
58	37043.5
59	34487.5
60	31463.0
61	27186.0
62	23337.5
63	20072.5
64	16683.0
65	13787.0
66	11252.0
67	8547.0
68	6192.0
69	4623.5
70	3309.0
71	2530.5
72	2010.5
73	1493.0
74	1136.5
75	896.0
76	612.5
77	428.5
78	328.5
79	209.5
80	122.0
81	63.5
82	48.0
83	38.5
84	24.5
85	14.5
86	13.0
87	12.0
88	7.5
89	5.5
90	6.5
91	7.0
92	5.5
93	3.0
94	1.5
95	1.0
96	2.0
97	1.5
98	1.5
99	1.0
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3342483494516942
2	0.12117628841308052
3	0.06571223446936103
4	0.03969762236580937
5	0.04223151315511635
6	0.00816475920998916
7	0.005743485789095823
8	0.013739318946464518
9	0.01565381420949646
10-11	0.018610020130354604
12-13	0.013851936314878162
14-15	0.016188746709461268
16-17	0.01692075960414995
18-19	0.01703337697256359
20-21	0.020862367498627476
22-23	0.013964553683291806
24-25	0.022326393288004842
26-27	0.01734307473570111
28-29	0.017061531314667006
30-31	0.017962470261976154
32-33	0.0219603868406605
34-35	0.013401466841223588
36-37	0.0087841547362642
38-39	0.011768514999225755
40-41	0.008671537367850556
42-43	0.007657981052127764
44-45	0.008221067894195983
46-47	0.007151202894266368
48-49	0.003575601447133184
50-51	0.0058842574996128775
52-53	0.006644424736404972
54-55	0.002252347368272872
56-57	0.00188634092092853
58-59	0.0017737235525148865
60-61	0.002730971184030857
62-63	0.0021115756577558173
64-65	0.001914495263031941
66-67	0.001379562763067134
68-69	0.0031251319734786095
70-71	0.0023086560524796934
72-73	0.0016329518419978322
74-75	0.0020834213156524064
76-77	0.0031532863155820205
78-79	0.0030688232892717884
80-81	0.0032377493418922535
82-83	0.0023649647366865154
84-85	0.0029562059208581445
86-87	0.0024212734208933374
88-89	0.6032349339076819
90-91	0.6139898925911849
92-93	0.6105269085124653
94-95	0.5970409786449314
96-97	0.5953798724608302
98-99	0.592620746934696
100	0.594338161803004
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1775925.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.74846598310918
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.2486972920526	48.4723725426986
2	13.22498570352256	16.59695131089181
3	4.249534254090212	7.999552667605108
4	1.8527138423300584	4.650198060475327
5	0.9541698464190461	2.9936347075067014
6	0.5944464686511568	2.2380362410161894
7	0.3978541463842547	1.7475316159441943
8	0.26933705294713256	1.3520389523875218
9	0.1872162377342998	1.0572778552460753
>10	0.9728550929087443	10.38426309510059
>50	0.03957190292939117	1.668258554124979
>100	0.008528432106397223	0.7951235947257234
>500	8.972792412370703E-5	0.04476080227716089
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	2.81543421034109E-5	0.0	0.0	0.0
16-17	0.0	5.63086842068218E-5	0.0	5.63086842068218E-5	0.0
18-19	0.0	5.63086842068218E-5	0.0	1.126173684136436E-4	0.0
20-21	0.0	5.63086842068218E-5	0.0	1.9708039472387628E-4	0.0
22-23	0.0	5.63086842068218E-5	0.0	2.533890789306981E-4	0.0
24-25	0.0	5.63086842068218E-5	0.0	4.504694736545744E-4	0.0
26-27	0.0	5.63086842068218E-5	0.0	6.757042104818616E-4	0.0
28-29	0.0	5.63086842068218E-5	0.0	0.0014358714472739557	0.0
30-31	0.0	5.63086842068218E-5	0.0	0.004870701183890085	0.0
32-33	0.0	5.63086842068218E-5	0.0	0.011627743288708702	0.0
34-35	0.0	5.63086842068218E-5	0.0	0.023368103945831047	0.0
36-37	0.0	5.63086842068218E-5	0.0	0.0428790630234948	0.0
38-39	0.0	5.63086842068218E-5	0.0	0.07736813210017315	0.0
40-41	0.0	5.63086842068218E-5	0.0	0.11914917578163492	0.0
42-43	0.0	5.63086842068218E-5	0.0	0.16059236735785576	0.0
44-45	0.0	5.63086842068218E-5	0.0	0.20963723130199755	0.0
46-47	5.63086842068218E-5	5.63086842068218E-5	0.0	0.2680293368244718	0.0
48-49	5.63086842068218E-5	5.63086842068218E-5	0.0	0.33354449089910887	0.0
50-51	5.63086842068218E-5	5.63086842068218E-5	0.0	0.4049157481312555	0.0
52-53	1.126173684136436E-4	5.63086842068218E-5	0.0	0.4789335135211228	0.0
54-55	1.126173684136436E-4	5.63086842068218E-5	0.0	0.5556540957529175	0.0
56-57	1.126173684136436E-4	5.63086842068218E-5	0.0	0.6492954375888622	0.0
58-59	1.126173684136436E-4	5.63086842068218E-5	0.0	0.7601109281078875	0.0
60-61	1.126173684136436E-4	5.63086842068218E-5	0.0	0.8748117178371835	0.0
62-63	1.126173684136436E-4	5.63086842068218E-5	0.0	0.9934259611188536	0.0
64-65	1.126173684136436E-4	5.63086842068218E-5	0.0	1.1122935934794544	0.0
66-67	1.126173684136436E-4	5.63086842068218E-5	0.0	1.2487858439967905	0.0
68-69	1.126173684136436E-4	5.63086842068218E-5	0.0	1.402987175697172	0.0
70-71	1.126173684136436E-4	5.63086842068218E-5	0.0	1.5775440967383194	0.0
72-73	1.126173684136436E-4	5.63086842068218E-5	0.0	1.7651927868575532	0.0
74-75	1.126173684136436E-4	5.63086842068218E-5	0.0	1.9434942353984543	0.0
76-77	1.126173684136436E-4	5.63086842068218E-5	0.0	2.133338964201754	0.0
78-79	1.126173684136436E-4	5.63086842068218E-5	0.0	2.3532806847136	0.0
80-81	1.126173684136436E-4	5.63086842068218E-5	0.0	2.5959992679871053	0.0
82-83	1.126173684136436E-4	5.63086842068218E-5	0.0	2.852034855075524	0.0
84-85	1.126173684136436E-4	5.63086842068218E-5	0.0	3.1072258119008405	0.0
86-87	1.126173684136436E-4	5.63086842068218E-5	0.0	3.3751143770147953	0.0
88	1.126173684136436E-4	5.63086842068218E-5	0.0	3.59587257344764	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2580	0.0	32.513367	1
GGTATCA	1810	0.0	32.024933	1
TCAACGC	2680	0.0	29.9744	4
ATCAACG	2785	0.0	29.020342	3
GTACATG	6165	0.0	28.894846	1
TACATGG	5895	0.0	28.634624	2
CAACGCA	2845	0.0	28.235193	5
AACGCAG	2940	0.0	27.472553	6
ACATGGG	6060	0.0	26.673811	3
TATCAAC	3485	0.0	23.746016	2
ACGCAGA	3565	0.0	22.26102	7
CGCAGAG	3605	0.0	22.014017	8
CATGGGG	4325	0.0	21.615044	4
GTCTAGG	370	0.0	20.378862	1
TACACCG	280	1.8117134E-9	20.132612	5
CTACGCT	170	8.662691E-5	19.343643	4
TACTTCG	230	2.297469E-6	18.37522	9
TATACCG	130	0.00806421	18.06773	5
GCAGAGT	4300	0.0	17.909904	9
GAGTACA	4245	0.0	17.762497	1
>>END_MODULE