FastQCFastQC Report
Wed 25 May 2016
SRR1033124_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033124_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1775925
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46790.2634683334037192No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39660.22332024156425526No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20710.11661528499232796No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31550.045.949661
GTATCAA54950.041.2010571
TCAACGC64900.034.851984
ATCAACG65050.034.699323
CAACGCA66450.033.9682585
AACGCAG68350.033.0732356
TATCAAC69800.032.4736332
ACGCAGA78250.028.6486537
CGCAGAG79700.027.771298
GTACATG61650.026.3400751
TACATGG61150.024.762742
GCAGAGT88700.024.58269
ACATGGG62850.023.2692383
GTACGAC1554.222943E-521.237013
GTGGTAT17600.020.8599321
GAGTACT54200.020.76537112-13
AGAGTAC78850.020.26332510-11
CAGAGTA86050.020.2334910-11
GAGTACA44800.019.8570521
CATGGGG43600.019.413914
Produced by FastQC (version 0.11.5)