##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033124_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1775925
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.764006925968154	34.0	31.0	34.0	31.0	34.0
2	32.85809704801723	34.0	31.0	34.0	31.0	34.0
3	32.94186353590383	34.0	31.0	34.0	31.0	34.0
4	36.36932190267044	37.0	37.0	37.0	35.0	37.0
5	36.306469586271945	37.0	37.0	37.0	35.0	37.0
6	36.39299351042415	37.0	37.0	37.0	35.0	37.0
7	36.36797049424948	37.0	37.0	37.0	35.0	37.0
8	36.3697042386362	37.0	37.0	37.0	35.0	37.0
9	38.18923659501387	39.0	39.0	39.0	37.0	39.0
10-11	38.164617030561544	39.0	39.0	39.0	37.0	39.0
12-13	38.12730774103636	39.0	39.0	39.0	37.0	39.0
14-15	39.66664611399693	41.0	40.0	41.0	37.0	41.0
16-17	39.67572110308713	41.0	40.0	41.0	37.0	41.0
18-19	39.68819235046525	41.0	40.0	41.0	37.0	41.0
20-21	39.67959119895266	41.0	40.0	41.0	37.0	41.0
22-23	39.608945479116514	41.0	40.0	41.0	37.0	41.0
24-25	39.55570308430818	41.0	40.0	41.0	37.0	41.0
26-27	39.45518617058716	41.0	39.0	41.0	36.5	41.0
28-29	39.36607936709039	41.0	39.0	41.0	36.0	41.0
30-31	39.269745907062514	41.0	39.0	41.0	36.0	41.0
32-33	39.30259611188535	41.0	39.0	41.0	35.5	41.0
34-35	39.2396064022974	41.0	39.0	41.0	35.0	41.0
36-37	39.155364387572675	41.0	39.0	41.0	35.0	41.0
38-39	39.06879513492969	41.0	39.0	41.0	35.0	41.0
40-41	38.95591790193842	41.0	39.0	41.0	35.0	41.0
42-43	38.83555780790292	41.0	39.0	41.0	35.0	41.0
44-45	38.681011303968354	41.0	39.0	41.0	35.0	41.0
46-47	38.451666596280816	41.0	38.0	41.0	35.0	41.0
48-49	38.24691752185481	41.0	38.0	41.0	34.5	41.0
50-51	38.09472641018061	40.0	38.0	41.0	34.0	41.0
52-53	37.94758731365345	40.0	37.5	41.0	34.0	41.0
54-55	37.798303137801426	40.0	37.0	41.0	34.0	41.0
56-57	37.62066416093022	40.0	37.0	41.0	33.5	41.0
58-59	37.41680166673706	40.0	36.0	41.0	33.0	41.0
60-61	37.210512268254575	39.5	36.0	41.0	33.0	41.0
62-63	36.95937103199741	39.0	35.0	41.0	33.0	41.0
64-65	36.70033391049735	39.0	35.0	41.0	33.0	41.0
66-67	36.40900094317046	38.5	35.0	41.0	32.0	41.0
68-69	36.0817160071512	37.0	35.0	40.0	32.0	41.0
70-71	35.748597772991545	37.0	35.0	40.0	31.5	41.0
72-73	35.398385066936946	36.5	35.0	39.0	31.0	41.0
74-75	35.06467784394049	36.0	35.0	39.0	31.0	41.0
76-77	34.74016554753157	35.5	35.0	38.5	31.0	40.0
78-79	34.41451666033194	35.0	35.0	37.0	31.0	39.0
80-81	34.09484578459113	35.0	35.0	37.0	31.0	39.0
82-83	33.79325140419781	35.0	34.0	36.5	30.0	39.0
84-85	33.543079240395855	35.0	34.0	36.0	30.0	37.0
86-87	33.32956797162042	35.0	34.0	36.0	30.0	37.0
88-89	33.12633500851669	35.0	34.0	35.0	30.0	37.0
90-91	32.949869786167774	35.0	34.0	35.0	30.0	36.0
92-93	32.795827526500275	35.0	34.0	35.0	29.5	36.0
94-95	32.65360699353858	35.0	34.0	35.0	29.0	36.0
96-97	32.534934752312175	35.0	34.0	35.0	29.0	36.0
98-99	32.4363084026634	35.0	34.0	35.0	29.0	35.0
100	32.341610709911734	35.0	34.0	35.0	29.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.10657004034754891
1101	2	0.10664230928551177
1101	3	0.1412292961293744
1101	4	0.06950062888316921
1101	5	0.0011815377343680211
1101	6	0.05289441664621819
1101	7	0.051765965159674465
1101	8	0.07359591084677675
1101	9	0.16083987834510083
1101	10-11	0.1543130758129294
1101	12-13	0.1192909584608941
1101	14-15	0.13377650694744148
1101	16-17	0.14277508714504705
1101	18-19	0.1904876313114343
1101	20-21	0.17710351187730566
1101	22-23	0.16106211289733352
1101	24-25	0.16338040793225161
1101	26-27	0.1532650770830557
1101	28-29	0.16033764126036942
1101	30-31	0.1142714246277805
1101	32-33	0.16360251458299047
1101	34-35	0.20370175635189014
1101	36-37	0.2620329766189897
1101	38-39	0.23730819775965273
1101	40-41	0.2426453723457982
1101	42-43	0.18117735349500919
1101	44-45	0.07174274582839502
1101	46-47	0.06259417922665023
1101	48-49	-0.039151310902404646
1101	50-51	-0.04511979996741644
1101	52-53	-0.02296276772997885
1101	54-55	-0.03437634948187451
1101	56-57	-0.07627960026274394
1101	58-59	-0.10918709106467617
1101	60-61	-0.09469139028430362
1101	62-63	-0.12753472621928807
1101	64-65	-0.056257604385237414
1101	66-67	-0.07272029002337632
1101	68-69	-0.06197249909599378
1101	70-71	-0.03603343258040326
1101	72-73	-0.009690863926842042
1101	74-75	-0.07670953849005713
1101	76-77	-0.036576762909639626
1101	78-79	-0.03360074795480017
1101	80-81	-0.04598635276609997
1101	82-83	-0.03490308323820557
1101	84-85	-0.017239499427098792
1101	86-87	-0.015706148946840415
1101	88-89	-0.07251528879491076
1101	90-91	-0.07077529788715964
1101	92-93	-0.0027882739674467416
1101	94-95	-0.030045678217994976
1101	96-97	-0.08592965112563888
1101	98-99	-0.0945977063327561
1101	100	-0.09152145945961365
1102	1	0.11139733985054079
1102	2	0.13524876617149317
1102	3	0.14857353400370954
1102	4	0.05771449919757288
1102	5	0.04790568462490086
1102	6	0.07692345550390911
1102	7	0.08713301506347904
1102	8	0.10977515573640062
1102	9	0.1660622087031669
1102	10-11	0.15232669451368963
1102	12-13	0.11689254538467964
1102	14-15	0.21264041552995394
1102	16-17	0.18779537277336544
1102	18-19	0.16847713115713958
1102	20-21	0.24716350909300644
1102	22-23	0.1672909459476415
1102	24-25	0.17426981918070794
1102	26-27	0.22598194950364103
1102	28-29	0.19287836809147763
1102	30-31	0.20641954884870728
1102	32-33	0.2298550270305455
1102	34-35	0.25042453996095304
1102	36-37	0.2701112212770127
1102	38-39	0.2551107291648478
1102	40-41	0.2381240894369796
1102	42-43	0.15555415743717305
1102	44-45	0.16154410128302032
1102	46-47	0.17001097575256097
1102	48-49	0.21875042890136598
1102	50-51	0.18436016749345896
1102	52-53	0.20194964544503335
1102	54-55	0.18246206926558983
1102	56-57	0.18386930856676287
1102	58-59	0.2230400329118183
1102	60-61	0.23618552368269263
1102	62-63	0.2872973912808163
1102	64-65	0.17911566849753058
1102	66-67	0.1936854392231453
1102	68-69	0.24932528712735547
1102	70-71	0.2896163394468587
1102	72-73	0.18446680133975946
1102	74-75	0.14322384997330317
1102	76-77	0.0924202179832534
1102	78-79	0.07903396831264331
1102	80-81	0.12718672175117973
1102	82-83	0.054702039877341235
1102	84-85	0.08430580418049516
1102	86-87	0.08904535210625397
1102	88-89	0.16879715992164535
1102	90-91	0.15248401621224872
1102	92-93	0.1238834298906113
1102	94-95	0.10298854046775574
1102	96-97	0.10862272422392749
1102	98-99	0.08207683635848184
1102	100	0.045292817978747735
1103	1	0.11671097167532452
1103	2	0.11180816227796697
1103	3	0.05762323621704013
1103	4	0.03842806453569381
1103	5	0.014236206931926176
1103	6	0.11021481367181707
1103	7	0.06975479761715064
1103	8	0.09395619406353006
1103	9	0.09193596886093758
1103	10-11	0.05482409211580119
1103	12-13	0.04310887403588026
1103	14-15	0.05575917032076916
1103	16-17	0.14890496101133266
1103	18-19	0.12416518707080115
1103	20-21	0.1011050582073807
1103	22-23	0.08855702733205817
1103	24-25	0.07440026651870824
1103	26-27	0.19598755970591952
1103	28-29	0.15265705356401327
1103	30-31	0.16650844825738176
1103	32-33	0.09769149415613754
1103	34-35	0.14150873104088646
1103	36-37	0.1800157854681501
1103	38-39	0.11561004028565236
1103	40-41	0.14937499302570245
1103	42-43	0.16714543096868084
1103	44-45	0.16157926250071597
1103	46-47	0.137767593389988
1103	48-49	0.13591902000150924
1103	50-51	0.12351387518539525
1103	52-53	0.04756508058029851
1103	54-55	0.0027328932823706964
1103	56-57	0.03756667822701587
1103	58-59	0.049374412817627444
1103	60-61	0.10813023995739712
1103	62-63	0.03484016010742863
1103	64-65	0.08565138262731864
1103	66-67	0.1334426568622007
1103	68-69	0.0673523420861386
1103	70-71	0.13256030166547106
1103	72-73	0.12963876854050227
1103	74-75	0.14890998800236588
1103	76-77	0.0496346293735499
1103	78-79	0.12316503872926177
1103	80-81	0.06497062324613267
1103	82-83	-0.030287067161829384
1103	84-85	-0.017695329086606648
1103	86-87	-0.01873711175487358
1103	88-89	-0.03627857624977793
1103	90-91	-0.12526995692738296
1103	92-93	-0.08105540362391395
1103	94-95	-0.08291396301094522
1103	96-97	-0.02763354320465794
1103	98-99	4.06449307540413E-4
1103	100	-0.01948381239468233
1104	1	0.0527792948639032
1104	2	0.08715716311805721
1104	3	0.07981432156946511
1104	4	0.04960276360492344
1104	5	0.015028529065084228
1104	6	0.05235816256347903
1104	7	0.06914914506216263
1104	8	0.0642540465293564
1104	9	0.09181268505342643
1104	10-11	0.08231698775370688
1104	12-13	0.09090599104479935
1104	14-15	0.0910096199351429
1104	16-17	0.0925771504286459
1104	18-19	0.06842650021773977
1104	20-21	0.0919898659422671
1104	22-23	0.1073669822341401
1104	24-25	0.14174408428310414
1104	26-27	0.025048722425381698
1104	28-29	0.06549062497309421
1104	30-31	0.15224384450942807
1104	32-33	0.0752281381070361
1104	34-35	0.04972469455009332
1104	36-37	-0.02221264896397912
1104	38-39	-0.0028137618294366007
1104	40-41	0.025959182370222322
1104	42-43	0.028619696042511578
1104	44-45	0.07913370905801997
1104	46-47	0.048603375719004305
1104	48-49	0.01097573358577364
1104	50-51	0.060804497427433546
1104	52-53	0.23016545907523778
1104	54-55	0.1245297888960124
1104	56-57	0.12878494364052528
1104	58-59	0.1203667792298404
1104	60-61	0.024694397578699068
1104	62-63	0.04058241051458111
1104	64-65	0.004644746834678415
1104	66-67	0.08834732041719917
1104	68-69	0.03482596420382578
1104	70-71	0.043781857679988434
1104	72-73	0.03461030559994782
1104	74-75	0.024146471517518364
1104	76-77	0.017318956104453775
1104	78-79	0.012109453351051513
1104	80-81	0.04365495907547512
1104	82-83	0.05817490289218341
1104	84-85	0.08504519565052959
1104	86-87	0.06372239262299217
1104	88-89	0.0902407109180885
1104	90-91	0.0877731817904035
1104	92-93	0.038674554796237715
1104	94-95	0.003394786348373202
1104	96-97	0.017320160920270666
1104	98-99	0.12546066082190777
1104	100	0.10518053113800363
1105	1	0.06962775553127187
1105	2	0.07414192527986785
1105	3	0.07349211492865493
1105	4	0.10443257742986845
1105	5	0.04075594954609585
1105	6	0.03512068816034741
1105	7	0.008793927994844353
1105	8	0.036581850743381494
1105	9	0.024670850528018207
1105	10-11	0.02470598975452276
1105	12-13	0.04384735734553402
1105	14-15	0.05713330048622112
1105	16-17	0.08374894429243085
1105	18-19	0.061261723856631534
1105	20-21	0.07564498150222931
1105	22-23	0.1254479064791454
1105	24-25	0.03503535758432008
1105	26-27	0.10416842482694477
1105	28-29	0.16234727077109312
1105	30-31	0.16099093277388477
1105	32-33	0.12442003981506389
1105	34-35	0.10875459521371056
1105	36-37	0.0966229795308422
1105	38-39	0.11735261048546164
1105	40-41	0.14856642518402907
1105	42-43	0.16855330553843828
1105	44-45	0.19221803643977609
1105	46-47	0.28220700416688516
1105	48-49	0.30074417359962524
1105	50-51	0.27379641204390026
1105	52-53	0.30900924336330604
1105	54-55	0.32073573147688705
1105	56-57	0.2828832297504462
1105	58-59	0.2619032367991849
1105	60-61	0.22328004220907616
1105	62-63	0.1972051786729594
1105	64-65	0.15828155586589787
1105	66-67	0.21684477686585524
1105	68-69	0.22487633649524952
1105	70-71	0.31351535686734877
1105	72-73	0.30606825632487755
1105	74-75	0.2468990810779914
1105	76-77	0.33100631345141807
1105	78-79	0.29501910593772607
1105	80-81	0.3569081231125324
1105	82-83	0.39533392912566967
1105	84-85	0.3559056905135449
1105	86-87	0.3289052232113221
1105	88-89	0.3001909253743804
1105	90-91	0.2901543507092086
1105	92-93	0.3808740372847552
1105	94-95	0.33552054398635534
1105	96-97	0.3293050697690134
1105	98-99	0.28538168457932755
1105	100	0.3133286966712632
1106	1	0.07142421380086716
1106	2	0.10217269119905836
1106	3	0.07978346178789764
1106	4	0.07164562503608352
1106	5	0.052020289503403205
1106	6	-0.0034006934924732946
1106	7	0.027017407460078857
1106	8	0.10619753946387078
1106	9	0.10036771786298715
1106	10-11	0.08515061706039972
1106	12-13	0.0446934681454394
1106	14-15	0.15652402443955538
1106	16-17	0.11215880400795442
1106	18-19	0.10049023408299007
1106	20-21	0.0855985947717528
1106	22-23	0.1947592184741893
1106	24-25	0.15825694299299187
1106	26-27	0.14090041031516165
1106	28-29	0.13017027238605294
1106	30-31	0.13403664351967137
1106	32-33	0.12345938167207748
1106	34-35	0.17342970589807294
1106	36-37	0.12471575073191588
1106	38-39	0.1627919595740721
1106	40-41	0.14792356176599242
1106	42-43	0.1973519341341401
1106	44-45	0.2414149903561551
1106	46-47	0.21255452187934765
1106	48-49	0.2081719120226424
1106	50-51	0.16591901527139896
1106	52-53	0.1528081925785827
1106	54-55	0.08463812075649457
1106	56-57	0.15640269474797464
1106	58-59	0.1526452691204554
1106	60-61	0.17795290996890145
1106	62-63	0.1932881821353689
1106	64-65	0.16895197279280438
1106	66-67	0.21294635366440673
1106	68-69	0.21046363950842562
1106	70-71	0.19049052170393566
1106	72-73	0.21147150110788715
1106	74-75	0.25134375810109333
1106	76-77	0.18517667006793914
1106	78-79	0.1926961959888871
1106	80-81	0.16996558802587458
1106	82-83	0.22058320024581235
1106	84-85	0.16880362247497516
1106	86-87	0.12689201481509826
1106	88-89	0.13762246885957552
1106	90-91	0.2044035882137294
1106	92-93	0.21069651573898085
1106	94-95	0.18314256374274152
1106	96-97	0.16737206146200378
1106	98-99	0.15024597493241032
1106	100	0.16498790999231971
1107	1	-0.045171161982104024
1107	2	-0.04199412223113086
1107	3	-0.043353144090524154
1107	4	-0.013842317435830864
1107	5	-0.13204609746817653
1107	6	-0.13955502277513432
1107	7	-0.08436002248144803
1107	8	-0.04996970500877751
1107	9	-0.112381890700604
1107	10-11	-0.07820522537428332
1107	12-13	-0.0593395532450387
1107	14-15	-0.04133833469762038
1107	16-17	-0.008657530892726584
1107	18-19	-0.013784066538569562
1107	20-21	-0.12233897966549989
1107	22-23	-0.08443707230898667
1107	24-25	-0.02713011463561088
1107	26-27	-0.055956116077744866
1107	28-29	-0.08198001828613855
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2216	8	-0.08342693229676001
2216	9	-0.0854955431300155
2216	10-11	-0.09847639516195983
2216	12-13	-0.07049950160615026
2216	14-15	-0.18378825678927768
2216	16-17	-0.20143298517056252
2216	18-19	-0.16478843728103243
2216	20-21	-0.16760632903800854
2216	22-23	-0.18508356624600708
2216	24-25	-0.24532738493725503
2216	26-27	-0.21352199128563853
2216	28-29	-0.24805065330944842
2216	30-31	-0.13763551929285
2216	32-33	-0.16376938931696827
2216	34-35	-0.17278555879082802
2216	36-37	-0.22548439683971822
2216	38-39	-0.2748979373430842
2216	40-41	-0.27566589857720203
2216	42-43	-0.27577133400124865
2216	44-45	-0.17468210365415615
2216	46-47	-0.2496618963624755
2216	48-49	-0.27824378602802824
2216	50-51	-0.2533350559389973
2216	52-53	-0.22510716205154324
2216	54-55	-0.2014432973105471
2216	56-57	-0.18425347940544867
2216	58-59	-0.21428239952170713
2216	60-61	-0.17694736344487438
2216	62-63	-0.16557229228570947
2216	64-65	-0.19876829109777816
2216	66-67	-0.21469801995446858
2216	68-69	-0.16573183716590023
2216	70-71	-0.22270468051738845
2216	72-73	-0.20423440289119554
2216	74-75	-0.2742026915351303
2216	76-77	-0.20650166439437356
2216	78-79	-0.2537147877054835
2216	80-81	-0.30043759572347994
2216	82-83	-0.27875723863336077
2216	84-85	-0.2952438602571661
2216	86-87	-0.30956054071558015
2216	88-89	-0.23680701372328627
2216	90-91	-0.27066252142198266
2216	92-93	-0.1239272427272553
2216	94-95	-0.24410737224972223
2216	96-97	-0.26934951470130386
2216	98-99	-0.2539190856409377
2216	100	-0.2958825784206667
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	7.0
3	7.0
4	12.0
5	18.0
6	45.0
7	110.0
8	197.0
9	375.0
10	643.0
11	1032.0
12	1586.0
13	2529.0
14	2932.0
15	3878.0
16	5208.0
17	7833.0
18	9631.0
19	5469.0
20	3627.0
21	3278.0
22	3485.0
23	3668.0
24	4079.0
25	4962.0
26	6403.0
27	8086.0
28	10164.0
29	13412.0
30	16721.0
31	21583.0
32	27871.0
33	36529.0
34	50862.0
35	82692.0
36	159577.0
37	366088.0
38	621311.0
39	288922.0
40	1093.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.87651870350613	19.154728108604385	13.315782680763549	23.652970507125936
2	15.799081924085304	19.865723514095485	40.0107439207878	24.32445064103142
3	18.830074468234866	25.062544870982727	29.64764840857581	26.459732252206596
4	12.507643916793644	16.414741187957578	37.85481569573438	33.22279919951439
5	13.00823450017857	37.69016017223839	35.17497045426636	14.126634873316682
6	31.542918599936936	37.43175368259832	17.27943826298482	13.745889454479931
7	28.02687830544819	32.42098458433566	21.75429764575506	17.79783946446108
8	25.958138809415082	36.0886235111213	19.45102164121409	18.502216038249536
9	25.741840013243827	16.22312241190773	20.567175810649342	37.4678617641991
10-11	24.712564554550724	26.329416209790924	28.03278637754336	20.92523285811499
12-13	25.41757112490674	23.580415839632867	28.710334051269058	22.29167898419134
14-15	22.342187873924857	25.355462646226613	26.187930233540268	26.114419246308263
16-17	21.780142742514464	29.031800329405804	26.620521699959177	22.56753522812056
18-19	21.67904660361406	27.471072109202844	29.307073583364605	21.54280770381849
20-21	22.522375663386683	26.632008671537367	29.29273477202021	21.552880893055733
22-23	22.246575789850954	26.373280785936082	29.360435897890703	22.019707526322264
24-25	22.015969710424063	26.49712867861218	29.388777507151907	22.098124103811845
26-27	21.906373959650445	26.651868581971282	29.334302979522274	22.107454478856
28-29	21.830461839004663	26.73168860985961	29.359296684038576	22.078552867097148
30-31	21.95012728838148	26.90495322056926	29.182737898591192	21.96218159245806
32-33	21.924095177990548	26.730700289246784	29.332625916410464	22.0125786163522
34-35	22.00560711605266	26.57978593827666	29.38517451479859	22.029432430872088
36-37	22.258118931693648	26.722049356661316	29.015899782845562	22.003931928799478
38-39	22.101463458043753	26.958808191444994	28.93851910448207	22.001209246029187
40-41	21.99909371744543	26.82140122322798	29.055818612813166	22.123686446513418
42-43	22.095229942042447	26.839661654717606	28.88170728238742	22.183401120852526
44-45	22.087746436077207	26.995671449164803	28.822358763111882	22.094223351646107
46-47	22.219481419469254	27.02145231946534	28.608180000906717	22.150886260158686
48-49	22.32560365933495	27.131366914780237	28.196814553993914	22.346214871890897
50-51	22.269090581449536	27.274222136500768	28.124582049271375	22.332105232778318
52-53	22.242835507019702	27.32174678395685	28.09882288414361	22.33659482487984
54-55	22.320910209448407	27.432504342978454	28.068676778227953	22.17790866934519
56-57	22.211388779475183	27.288506906014735	28.04441404948928	22.4556902650208
58-59	22.0737471465269	26.998782593222842	28.309409394416146	22.618060865834117
60-61	22.310280140175667	27.211360682059865	28.139248820818796	22.339110356945675
62-63	22.048142160597372	27.21561433115585	28.215159915468547	22.521083592778226
64-65	22.12235573417213	27.086004557763488	28.22674056843093	22.564899139633454
66-67	22.079347355416623	27.160057953289378	28.14276375025128	22.61783094104272
68-69	22.310433922544824	27.155091954550485	28.089714283622797	22.444759839281897
70-71	22.213381713999986	27.125016048056327	28.024213983093794	22.637388254849892
72-73	22.171422103935747	27.254915706809086	27.971307177231786	22.602355012023384
74-75	22.208651760556243	27.27795120940405	27.88549683656209	22.62790019347762
76-77	22.24160187621328	27.272888521003928	27.82178789273713	22.663721710045653
78-79	22.300863578378472	27.29468510626437	27.877142299686724	22.52730901567043
80-81	22.12593388426029	27.29447789197237	27.9948207009791	22.58476752278824
82-83	22.155708853510607	27.28187747512975	27.956430396240368	22.605983275119275
84-85	22.13438668408487	27.226930267129152	27.831377539528805	22.807305509257173
86-87	22.197574611265196	27.338708383225274	27.78239353484937	22.68132347066016
88-89	22.06444528907471	27.219308247814517	27.839069780536903	22.877176682573868
90-91	22.00264650815772	27.17761166715937	27.87322099750834	22.94652082717457
92-93	22.0594056618382	27.28699128623112	27.820009290932894	22.83359376099779
94-95	21.914636034742458	27.178287371369848	27.89292903698073	23.014147556906963
96-97	21.980010417106577	27.128341568478398	27.80900094317046	23.082647071244562
98-99	21.889522361586216	27.248476146233653	27.79810521277644	23.063896279403693
100	22.04096456776046	26.960992159015724	27.679772512915807	23.318270760308007
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	41.0
1	45.0
2	172.0
3	332.5
4	237.5
5	159.5
6	415.5
7	949.0
8	1640.0
9	1909.0
10	1437.0
11	906.5
12	827.5
13	938.5
14	1052.5
15	1211.5
16	1371.0
17	1541.0
18	1731.0
19	1955.5
20	2323.5
21	2789.0
22	3516.5
23	4771.5
24	6285.5
25	8033.5
26	10547.5
27	13506.0
28	17064.0
29	21258.5
30	25157.0
31	29102.0
32	33114.5
33	36905.0
34	40818.5
35	44461.0
36	47649.0
37	51239.5
38	54479.5
39	56452.0
40	58683.0
41	60490.5
42	61099.5
43	61908.5
44	62335.5
45	61876.0
46	61342.0
47	60140.0
48	59330.5
49	59016.0
50	57677.0
51	55847.5
52	52807.5
53	50339.0
54	49305.5
55	47181.5
56	43919.0
57	40149.5
58	36811.0
59	34502.0
60	31530.0
61	27086.5
62	23169.0
63	19811.5
64	16433.5
65	13457.5
66	10748.5
67	8192.0
68	6042.5
69	4468.0
70	3124.5
71	2295.0
72	1794.0
73	1372.0
74	1069.0
75	808.5
76	530.0
77	369.5
78	256.0
79	135.0
80	77.0
81	43.5
82	12.0
83	3.0
84	2.0
85	1.5
86	1.5
87	0.5
88	0.5
89	1.0
90	1.5
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09189577262553317
2	0.0020834213156524064
3	0.0
4	1.689260526204654E-4
5	0.0400354744710503
6	2.81543421034109E-4
7	0.003885299210270704
8	4.504694736545744E-4
9	1.689260526204654E-4
10-11	2.533890789306981E-4
12-13	0.0
14-15	0.0
16-17	0.0
18-19	2.81543421034109E-5
20-21	0.0
22-23	2.533890789306981E-4
24-25	2.81543421034109E-5
26-27	0.001098019342033025
28-29	0.009319087236229007
30-31	0.03522108197136704
32-33	0.025423370919380038
34-35	0.02829511381392795
36-37	0.02593014907724144
38-39	0.02469135802469136
40-41	0.030434843813787183
42-43	0.02260793670903895
44-45	0.021875923814350266
46-47	0.01630136407787491
48-49	0.010839421709813196
50-51	0.008305530920506215
52-53	0.005715331446992412
54-55	0.003941607894477526
56-57	0.002562045131410392
58-59	0.0010698649999296142
60-61	5.912411841716288E-4
62-63	0.0010135563157227924
64-65	0.003040668947168377
66-67	6.757042104818616E-4
68-69	0.0011543280262398467
70-71	5.067781578613962E-4
72-73	5.912411841716288E-4
74-75	5.067781578613962E-4
76-77	4.223151315511635E-4
78-79	2.81543421034109E-5
80-81	5.067781578613962E-4
82-83	4.504694736545744E-4
84-85	2.81543421034109E-4
86-87	1.9708039472387628E-4
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1775925.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.71187996623405
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.10989397690075	46.20774747368399
2	13.870037639085808	16.841521205426517
3	4.450287936975076	8.105560411344303
4	1.9066443459132294	4.630238506695314
5	1.0532742757834148	3.197313070144239
6	0.624938677031152	2.2764721167703446
7	0.41775249637212003	1.7753777590737145
8	0.27743818228024414	1.3475034896518676
9	0.1939551929795063	1.0597845955499594
>10	1.0379801631461207	10.834184975053438
>50	0.045556094720064624	1.8500626700710756
>100	0.011777530633942086	1.1619733173734497
>500	9.269763572977221E-5	0.04491022491396535
>1k	3.7079054291908883E-4	0.6673501842478712
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4679	0.2634683334037192	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3966	0.22332024156425526	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2071	0.11661528499232796	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	5.63086842068218E-5	0.0	0.0	0.0
4	0.0	5.63086842068218E-5	0.0	0.0	0.0
5	0.0	5.63086842068218E-5	0.0	0.0	0.0
6	0.0	5.63086842068218E-5	0.0	0.0	0.0
7	0.0	5.63086842068218E-5	0.0	0.0	0.0
8	0.0	5.63086842068218E-5	0.0	0.0	0.0
9	0.0	5.63086842068218E-5	0.0	0.0	0.0
10-11	0.0	5.63086842068218E-5	0.0	0.0	0.0
12-13	0.0	5.63086842068218E-5	0.0	2.81543421034109E-5	0.0
14-15	0.0	5.63086842068218E-5	0.0	5.63086842068218E-5	0.0
16-17	2.81543421034109E-5	5.63086842068218E-5	0.0	1.126173684136436E-4	0.0
18-19	5.63086842068218E-5	5.63086842068218E-5	0.0	1.689260526204654E-4	0.0
20-21	5.63086842068218E-5	5.63086842068218E-5	0.0	2.533890789306981E-4	0.0
22-23	5.63086842068218E-5	5.63086842068218E-5	0.0	3.096977631375199E-4	0.0
24-25	5.63086842068218E-5	5.63086842068218E-5	0.0	5.067781578613962E-4	0.0
26-27	5.63086842068218E-5	5.63086842068218E-5	0.0	7.320128946886834E-4	0.0
28-29	5.63086842068218E-5	5.63086842068218E-5	0.0	0.0014921801314807777	0.0
30-31	5.63086842068218E-5	5.63086842068218E-5	0.0	0.004955164210200318	0.0
32-33	5.63086842068218E-5	5.63086842068218E-5	0.0	0.011740360657122344	0.0
34-35	5.63086842068218E-5	5.63086842068218E-5	0.0	0.023424412630037868	0.0
36-37	5.63086842068218E-5	5.63086842068218E-5	0.0	0.04293537170770162	0.0
38-39	5.63086842068218E-5	5.63086842068218E-5	0.0	0.0775089038106902	0.0
40-41	5.63086842068218E-5	5.63086842068218E-5	0.0	0.1193744105184622	0.0
42-43	5.63086842068218E-5	1.126173684136436E-4	0.0	0.16095837380520012	0.0
44-45	5.63086842068218E-5	1.126173684136436E-4	0.0	0.21034108985458283	0.0
46-47	5.63086842068218E-5	1.126173684136436E-4	0.0	0.26878950406126384	0.0
48-49	5.63086842068218E-5	1.126173684136436E-4	0.0	0.3342483494516942	0.0
50-51	5.63086842068218E-5	1.126173684136436E-4	0.0	0.40559145234173744	0.0
52-53	5.63086842068218E-5	1.126173684136436E-4	0.0	0.47958106338950124	0.0
54-55	5.63086842068218E-5	1.126173684136436E-4	0.0	0.5562734912791926	0.0
56-57	5.63086842068218E-5	1.126173684136436E-4	0.0	0.6500274504835508	0.0
58-59	5.63086842068218E-5	1.126173684136436E-4	0.0	0.7612934104762308	0.0
60-61	5.63086842068218E-5	1.126173684136436E-4	0.0	0.8762757436265608	0.0
62-63	5.63086842068218E-5	1.126173684136436E-4	0.0	0.9948899869082309	0.0
64-65	5.63086842068218E-5	1.126173684136436E-4	0.0	1.113363458479384	0.0
66-67	5.63086842068218E-5	1.126173684136436E-4	0.0	1.2505032588650984	0.0
68-69	5.63086842068218E-5	1.126173684136436E-4	0.0	1.4049579796444105	0.0
70-71	1.126173684136436E-4	1.126173684136436E-4	0.0	1.579768289764489	0.0
72-73	1.689260526204654E-4	1.126173684136436E-4	0.0	1.7683179188310318	0.0
74-75	1.689260526204654E-4	1.126173684136436E-4	0.0	1.9468446021087602	0.0
76-77	1.689260526204654E-4	1.126173684136436E-4	0.0	2.1373368807804383	0.0
78-79	1.689260526204654E-4	1.126173684136436E-4	0.0	2.3598124920815913	0.0
80-81	1.689260526204654E-4	1.126173684136436E-4	0.0	2.605965905091713	0.0
82-83	1.689260526204654E-4	1.126173684136436E-4	0.0	2.8651547784957136	0.0
84-85	1.689260526204654E-4	1.126173684136436E-4	0.0	3.123133015189268	0.0
86-87	1.689260526204654E-4	1.126173684136436E-4	0.0	3.3944564100398384	0.0
88	1.689260526204654E-4	1.126173684136436E-4	0.0	3.61766403423568	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3155	0.0	45.94966	1
GTATCAA	5495	0.0	41.201057	1
TCAACGC	6490	0.0	34.85198	4
ATCAACG	6505	0.0	34.69932	3
CAACGCA	6645	0.0	33.968258	5
AACGCAG	6835	0.0	33.073235	6
TATCAAC	6980	0.0	32.473633	2
ACGCAGA	7825	0.0	28.648653	7
CGCAGAG	7970	0.0	27.77129	8
GTACATG	6165	0.0	26.340075	1
TACATGG	6115	0.0	24.76274	2
GCAGAGT	8870	0.0	24.5826	9
ACATGGG	6285	0.0	23.269238	3
GTACGAC	155	4.222943E-5	21.23701	3
GTGGTAT	1760	0.0	20.859932	1
GAGTACT	5420	0.0	20.765371	12-13
AGAGTAC	7885	0.0	20.263325	10-11
CAGAGTA	8605	0.0	20.23349	10-11
GAGTACA	4480	0.0	19.857052	1
CATGGGG	4360	0.0	19.41391	4
>>END_MODULE