FastQCFastQC Report
Wed 25 May 2016
SRR1033123_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033123_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2862127
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA42300.035.4206961
GGTATCA25450.034.4346351
TCAACGC42700.034.1039054
ATCAACG42750.033.851353
CAACGCA45250.032.1814655
AACGCAG47750.030.484856
TATCAAC51450.028.4150922
GTACATG97750.027.7154161
TACATGG96500.027.4255372
ACGCAGA55650.025.9041277
ACATGGG99850.025.5513113
CGCAGAG57950.024.5518918
CATGGGG63350.020.8367524
GCAGAGT68700.020.1632739
GAGTACA69150.020.100191
CATGGGT26750.019.8438054
TACACCG3801.2732926E-1118.542595
AGTACAT71750.018.2136482
TATACTG17950.017.7953765
CGTATAC2152.6316484E-517.4828783
Produced by FastQC (version 0.11.5)