##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033123_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2862127
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.6599273896651	33.0	31.0	34.0	30.0	34.0
2	31.72947252165959	34.0	31.0	34.0	30.0	34.0
3	31.889546480641844	34.0	31.0	34.0	30.0	34.0
4	35.242548286641366	37.0	35.0	37.0	33.0	37.0
5	35.33591276697365	37.0	35.0	37.0	33.0	37.0
6	35.469917302761196	37.0	37.0	37.0	35.0	37.0
7	35.37026064881118	37.0	36.0	37.0	35.0	37.0
8	35.385501062671224	37.0	36.0	37.0	35.0	37.0
9	37.0521437378565	39.0	38.0	39.0	35.0	39.0
10-11	37.04924886282125	39.0	38.0	39.0	35.0	39.0
12-13	37.01588556342888	39.0	38.0	39.0	35.0	39.0
14-15	38.482429151466725	41.0	39.0	41.0	35.0	41.0
16-17	38.49318024671861	41.0	39.0	41.0	35.0	41.0
18-19	38.486154877124605	41.0	39.0	41.0	34.0	41.0
20-21	38.53636176871257	41.0	39.0	41.0	34.5	41.0
22-23	38.49735458978584	41.0	39.0	41.0	34.0	41.0
24-25	38.50074629812025	41.0	39.0	41.0	34.0	41.0
26-27	38.47405216470129	41.0	39.0	41.0	34.0	41.0
28-29	38.451901505418874	40.5	39.0	41.0	34.0	41.0
30-31	38.44805611351278	41.0	39.0	41.0	34.0	41.0
32-33	38.518983783738456	41.0	39.0	41.0	34.0	41.0
34-35	38.4921740020621	41.0	39.0	41.0	34.0	41.0
36-37	38.41023022388595	41.0	39.0	41.0	34.0	41.0
38-39	38.3300309525049	41.0	39.0	41.0	34.0	41.0
40-41	38.26805274538831	41.0	39.0	41.0	33.5	41.0
42-43	38.149022038504924	40.5	38.0	41.0	33.0	41.0
44-45	38.01474567690393	40.0	38.0	41.0	33.0	41.0
46-47	37.89083066544566	40.0	38.0	41.0	33.0	41.0
48-49	37.771519747376686	40.0	38.0	41.0	33.0	41.0
50-51	37.64863997998691	40.0	38.0	41.0	32.5	41.0
52-53	37.50588478428805	40.0	37.0	41.0	32.0	41.0
54-55	37.35854296472519	40.0	37.0	41.0	32.0	41.0
56-57	37.20227351895985	40.0	36.5	41.0	31.5	41.0
58-59	37.00658216773749	40.0	36.0	41.0	31.0	41.0
60-61	36.789063692841026	39.5	35.5	41.0	31.0	41.0
62-63	36.52759136823768	39.0	35.0	41.0	31.0	41.0
64-65	36.256641127385336	39.0	35.0	41.0	31.0	41.0
66-67	35.959123232477104	38.0	35.0	40.5	30.0	41.0
68-69	35.64681511337547	37.5	35.0	40.0	30.0	41.0
70-71	35.29348260926227	37.0	35.0	40.0	30.0	41.0
72-73	34.92006504253655	36.5	34.5	39.0	29.0	41.0
74-75	34.54946600902056	36.0	34.0	39.0	29.0	41.0
76-77	34.21334692695328	35.5	34.0	38.5	29.0	40.0
78-79	33.846737408927	35.0	34.0	37.0	29.0	39.0
80-81	33.489439147878485	35.0	34.0	37.0	29.0	39.0
82-83	33.268326492849546	35.0	34.0	36.5	29.0	39.0
84-85	33.0017464284429	35.0	34.0	36.0	28.5	37.5
86-87	32.75196995101894	35.0	34.0	36.0	28.0	37.0
88-89	32.39295146581546	35.0	34.0	35.5	27.0	37.0
90-91	32.20889062574791	35.0	34.0	35.0	27.0	36.0
92-93	32.033290276776675	35.0	34.0	35.0	27.0	36.0
94-95	31.86579229363337	35.0	33.5	35.0	26.5	36.0
96-97	31.692061882648815	35.0	33.0	35.0	26.0	36.0
98-99	31.50080202590591	35.0	33.0	35.0	25.0	35.0
100	31.339064269335356	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.041493602511302186
1101	2	0.05275016819470579
1101	3	0.11188574144908969
1101	4	0.014362197290317624
1101	5	0.02162883152887929
1101	6	0.0023710775887693103
1101	7	-0.011270275041056266
1101	8	-0.005207354353906624
1101	9	0.07608313039988701
1101	10-11	0.04449982136406305
1101	12-13	0.09585996598912061
1101	14-15	0.12765495713570374
1101	16-17	0.0315431181462813
1101	18-19	0.05162052920116622
1101	20-21	0.0033548370603426747
1101	22-23	0.03122652138745252
1101	24-25	0.01991898238540557
1101	26-27	0.026795944078088496
1101	28-29	0.09029839833215902
1101	30-31	0.12861678841674973
1101	32-33	0.11915912926964722
1101	34-35	0.14539682602887893
1101	36-37	0.13894785745588933
1101	38-39	0.08456608656291564
1101	40-41	0.08966786739863863
1101	42-43	0.060958000121011935
1101	44-45	-0.041382683480506444
1101	46-47	0.02701141050678757
1101	48-49	0.0034973364675252583
1101	50-51	0.004689979788231824
1101	52-53	-0.020308094469648097
1101	54-55	-2.069800826305368E-4
1101	56-57	-0.05417428149245751
1101	58-59	0.006743724629167502
1101	60-61	0.09939147557426509
1101	62-63	0.022741757643068183
1101	64-65	0.04733561632836114
1101	66-67	0.03865192482210489
1101	68-69	0.021839230618496686
1101	70-71	0.035505839902981506
1101	72-73	0.07959041162268221
1101	74-75	0.02437298415948419
1101	76-77	-0.004217074862708614
1101	78-79	0.05224004261992121
1101	80-81	0.007571219723580214
1101	82-83	-0.0506625318287206
1101	84-85	-0.03505543005240952
1101	86-87	0.05246649541030024
1101	88-89	0.14921203550656514
1101	90-91	0.13291571454576712
1101	92-93	0.1535470789526272
1101	94-95	0.15830142450147733
1101	96-97	0.16815711655624455
1101	98-99	0.16096374745808362
1101	100	0.24627660375114857
1102	1	0.16618736851877003
1102	2	0.17172047982862182
1102	3	0.227953030852742
1102	4	0.20223299907900838
1102	5	0.07905007266848685
1102	6	0.12510162182523032
1102	7	0.16411550828017596
1102	8	0.18030466939699608
1102	9	0.22534429056255334
1102	10-11	0.1959955523198289
1102	12-13	0.18606025919037705
1102	14-15	0.20064230371592373
1102	16-17	0.22849979584471924
1102	18-19	0.1769296622217098
1102	20-21	0.14402907624587158
1102	22-23	0.1557788148278405
1102	24-25	0.19973377186056496
1102	26-27	0.23324178469442103
1102	28-29	0.2349822509522781
1102	30-31	0.25154337136979876
1102	32-33	0.26290472757878547
1102	34-35	0.23156605298824218
1102	36-37	0.23510739264249025
1102	38-39	0.19072134149570985
1102	40-41	0.2673334463499941
1102	42-43	0.24342791271982378
1102	44-45	0.1661687819974773
1102	46-47	0.18153984687931057
1102	48-49	0.1713735420444351
1102	50-51	0.12383635905685964
1102	52-53	0.09114020581090188
1102	54-55	0.1556441861383675
1102	56-57	0.059353137256806576
1102	58-59	0.07690574292989538
1102	60-61	0.08483101074024546
1102	62-63	0.13053251533560228
1102	64-65	0.11992682683618483
1102	66-67	0.13375460324052568
1102	68-69	0.06989512954781674
1102	70-71	0.1553417368789951
1102	72-73	0.1400089900372663
1102	74-75	0.11122612403873688
1102	76-77	0.1409672144154399
1102	78-79	0.13760275998582472
1102	80-81	0.07463842898521733
1102	82-83	0.10473806101606442
1102	84-85	0.10779939358231871
1102	86-87	0.09522013652506445
1102	88-89	0.1977022088323679
1102	90-91	0.2505905471945411
1102	92-93	0.1582216005679058
1102	94-95	0.1412592628418743
1102	96-97	0.13133591752340834
1102	98-99	0.18360663797197674
1102	100	0.1780924956300325
1103	1	-0.020133388248229522
1103	2	-0.030199140561052218
1103	3	-0.024462217980936174
1103	4	0.060801977726420375
1103	5	-0.05032503776745756
1103	6	0.008805976468927668
1103	7	0.0925835011342997
1103	8	0.025416450129164048
1103	9	0.09304255777215076
1103	10-11	0.07960988187943485
1103	12-13	0.08881553587098523
1103	14-15	0.1297906378607223
1103	16-17	0.07730173581698097
1103	18-19	0.03306796359862574
1103	20-21	0.02850612549927689
1103	22-23	0.04293429659180248
1103	24-25	0.09151933682881719
1103	26-27	0.12402645698635695
1103	28-29	0.1174918485575418
1103	30-31	0.07554246938053666
1103	32-33	0.06973138558317515
1103	34-35	0.16527921767293208
1103	36-37	0.1083306855216577
1103	38-39	0.08664539062283438
1103	40-41	0.1018531861191434
1103	42-43	0.04581456461990285
1103	44-45	-0.018621765641867682
1103	46-47	0.024404818814453222
1103	48-49	-0.051773682742982885
1103	50-51	-0.05624457287287754
1103	52-53	-0.1379310420274038
1103	54-55	-0.1202076963979053
1103	56-57	-0.10401854924580078
1103	58-59	-0.08027929562132385
1103	60-61	-0.04784115974963754
1103	62-63	-0.015015353112538321
1103	64-65	-0.04866920155512133
1103	66-67	-0.08182567175443722
1103	68-69	-0.012598952699846677
1103	70-71	-0.013566399165917176
1103	72-73	0.07738508913489284
1103	74-75	-0.05611498572343976
1103	76-77	-0.05901320357661177
1103	78-79	-0.15407401122045172
1103	80-81	-0.10052340817716754
1103	82-83	-0.07853183878042813
1103	84-85	-0.031471863916614495
1103	86-87	-0.04853263174626932
1103	88-89	0.0860993538078958
1103	90-91	0.027378597475973265
1103	92-93	0.027793289777477526
1103	94-95	0.06144631385729227
1103	96-97	0.1083262200213504
1103	98-99	0.11641424344008655
1103	100	0.1679263043578949
1104	1	0.04674780697522962
1104	2	0.03201944314750094
1104	3	0.0784319557076607
1104	4	0.08042909605894977
1104	5	0.00613690594822458
1104	6	0.005244109600347713
1104	7	-0.031983394376972285
1104	8	-0.005216523995201783
1104	9	-0.014363643867014275
1104	10-11	-0.02235640012752782
1104	12-13	0.00763022759532106
1104	14-15	-0.020413109478440106
1104	16-17	-0.015636336868929845
1104	18-19	-0.023756122984806893
1104	20-21	-0.08700445882857366
1104	22-23	-0.11515891604845052
1104	24-25	-0.041588403944054164
1104	26-27	-0.050823418976619905
1104	28-29	0.056327261428684494
1104	30-31	0.01854349730778182
1104	32-33	0.013935844836183264
1104	34-35	-0.033777231368674165
1104	36-37	0.010070933114967318
1104	38-39	-0.03898089207694966
1104	40-41	-0.01889114914632728
1104	42-43	-0.06603933114658389
1104	44-45	-0.02828403427871251
1104	46-47	-0.04998001551181375
1104	48-49	-0.09975814294332253
1104	50-51	-0.08767288272436957
1104	52-53	-0.09419376298050963
1104	54-55	-0.12658902841911157
1104	56-57	-0.0800902553010232
1104	58-59	-0.036122697904964696
1104	60-61	-0.0566616980752741
1104	62-63	-0.16535289359512717
1104	64-65	-0.1731344708345901
1104	66-67	-0.1436317413325341
1104	68-69	-0.09419359376306602
1104	70-71	-0.08951433330787495
1104	72-73	-0.1064693128185965
1104	74-75	-0.20325730910765571
1104	76-77	-0.21623605381948607
1104	78-79	-0.12011070129474177
1104	80-81	-0.08042854406645716
1104	82-83	-0.05080484466166979
1104	84-85	-0.03626747323922075
1104	86-87	-0.03294687935513707
1104	88-89	0.10340674293632901
1104	90-91	0.0032364465912451124
1104	92-93	-0.023223432067815963
1104	94-95	0.04202872230354515
1104	96-97	0.080464902535347
1104	98-99	0.043029322864079234
1104	100	0.12596174027610374
1105	1	0.14271943507223384
1105	2	0.12449531512490353
1105	3	0.10040489336036273
1105	4	0.15617025317586553
1105	5	0.03499245167309084
1105	6	0.15948826056722964
1105	7	0.061108022892391034
1105	8	0.04768722636668343
1105	9	0.05985691345175326
1105	10-11	0.048959256909576254
1105	12-13	0.071098358583356
1105	14-15	0.14856489593836386
1105	16-17	0.10494729476712195
1105	18-19	0.10012946946739021
1105	20-21	0.09371828604345467
1105	22-23	0.18604227662128636
1105	24-25	0.15574256461307812
1105	26-27	0.17122744265149947
1105	28-29	0.19866331668976756
1105	30-31	0.20736539773284335
1105	32-33	0.20801186400331062
1105	34-35	0.17840190719260818
1105	36-37	0.15062833305165668
1105	38-39	0.16144606637170966
1105	40-41	0.1551853976310582
1105	42-43	0.16142629978057954
1105	44-45	0.19726900949129345
1105	46-47	0.20425507138153165
1105	48-49	0.16230147160356267
1105	50-51	0.19973366938323522
1105	52-53	0.12640887615302177
1105	54-55	0.18203117607720998
1105	56-57	0.12664671881359624
1105	58-59	0.09891542531773467
1105	60-61	0.16662025413677384
1105	62-63	0.22635002936566195
1105	64-65	0.17779500255627312
1105	66-67	0.19064587906407127
1105	68-69	0.14759767009346803
1105	70-71	0.07354530559025818
1105	72-73	0.10335079914300849
1105	74-75	0.17314660765438816
1105	76-77	0.2118114365144237
1105	78-79	0.21392273025527686
1105	80-81	0.19401333898805717
1105	82-83	0.11958147426926047
1105	84-85	0.09559179822571906
1105	86-87	0.18474646274464135
1105	88-89	0.2760137598820904
1105	90-91	0.3210306434196255
1105	92-93	0.2490390147991235
1105	94-95	0.27018058476772566
1105	96-97	0.3065678785277228
1105	98-99	0.3300513282700557
1105	100	0.41842151337663935
1106	1	0.11510028983157028
1106	2	0.11746490316199143
1106	3	0.08220914106346555
1106	4	-0.0052676333447223556
1106	5	0.07279995160617148
1106	6	0.06855128362207097
1106	7	0.04558642670557589
1106	8	0.0029753753790515702
1106	9	0.026847562637684064
1106	10-11	-0.010388581099597616
1106	12-13	-0.03875382099178637
1106	14-15	-0.025486208729518012
1106	16-17	-0.04861181218410593
1106	18-19	0.07717767903252337
1106	20-21	0.014712400081734245
1106	22-23	0.049366925516899585
1106	24-25	0.04732893205891742
1106	26-27	0.09257427696122278
1106	28-29	0.1204154011833296
1106	30-31	0.11501726766714881
1106	32-33	0.08662448563277536
1106	34-35	0.038356399041461486
1106	36-37	0.06643267672861697
1106	38-39	0.033349032225252984
1106	40-41	0.014354443513738602
1106	42-43	0.037172844775888336
1106	44-45	0.037472988772265126
1106	46-47	0.03467809522853571
1106	48-49	0.05681721817249752
1106	50-51	0.030389446171966483
1106	52-53	-0.006057145073093295
1106	54-55	0.024077374761397152
1106	56-57	0.013071145054205147
1106	58-59	3.2360781867168953E-4
1106	60-61	0.02174142277198854
1106	62-63	0.07251293009190363
1106	64-65	0.0207784153999242
1106	66-67	0.05645276354570683
1106	68-69	-0.030882650150203972
1106	70-71	0.0013107184813492267
1106	72-73	0.02181003886896349
1106	74-75	0.05504671496665736
1106	76-77	0.024259953935853673
1106	78-79	0.025347200021435867
1106	80-81	0.007753776694158887
1106	82-83	-0.04570467039099668
1106	84-85	-0.03321407689813327
1106	86-87	0.02080253759642403
1106	88-89	0.07064987639218145
1106	90-91	0.04561424525127222
1106	92-93	0.04030821147612329
1106	94-95	0.10109400567884208
1106	96-97	0.07335930577659866
1106	98-99	0.08682113093168908
1106	100	0.09051674251804798
1107	1	0.17149694916122016
1107	2	0.1406564395926111
1107	3	0.13323802139519003
1107	4	0.08582215814805494
1107	5	0.023715236708767407
1107	6	0.09379833276172178
1107	7	0.006431262597551779
1107	8	0.07984527953426124
1107	9	0.11055875890027522
1107	10-11	0.07187134524732386
1107	12-13	0.027353528705674535
1107	14-15	0.005861988274489249
1107	16-17	-0.029602206275718856
1107	18-19	0.03578065309722689
1107	20-21	1.4757254290742594E-4
1107	22-23	0.020635914691631285
1107	24-25	0.02421034910215525
1107	26-27	0.059446493148051616
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2216	6	-0.06662551099907432
2216	7	-0.03933939798373132
2216	8	-0.0741775265934379
2216	9	-0.19026333062414835
2216	10-11	-0.08744662899469091
2216	12-13	-0.09856773987199574
2216	14-15	-0.18272242138489503
2216	16-17	-0.11687634482664322
2216	18-19	-0.10674779962163683
2216	20-21	-0.1502601537976247
2216	22-23	-0.09268387830918101
2216	24-25	-0.1286532510205589
2216	26-27	-0.19987666750135702
2216	28-29	-0.17057637287016547
2216	30-31	-0.21081795604334985
2216	32-33	-0.1393159265379751
2216	34-35	-0.14189175060396053
2216	36-37	-0.1496448226974323
2216	38-39	-0.1421407675166364
2216	40-41	-0.12455015190850105
2216	42-43	-0.152600005489127
2216	44-45	-0.11858110383225551
2216	46-47	-0.05390556760121967
2216	48-49	-0.03583668023451736
2216	50-51	-0.05441069908723506
2216	52-53	-0.01663686394676489
2216	54-55	-0.01987800026224562
2216	56-57	-0.04633057640950966
2216	58-59	-0.07048493873816852
2216	60-61	-0.008048380325796245
2216	62-63	0.01941213741140757
2216	64-65	0.0246320734269716
2216	66-67	0.06266848367550182
2216	68-69	0.058657620939541744
2216	70-71	-0.0046341963688902865
2216	72-73	0.141333243781105
2216	74-75	0.11032212849198686
2216	76-77	0.07529215876198947
2216	78-79	0.048555801428292966
2216	80-81	0.06999418644244315
2216	82-83	0.07136719668528002
2216	84-85	0.09209240422672593
2216	86-87	0.04175799907328326
2216	88-89	0.03592777239184386
2216	90-91	0.10020763395763765
2216	92-93	0.09418581637955015
2216	94-95	0.02656116978862144
2216	96-97	0.056374536434468325
2216	98-99	0.057119403445142325
2216	100	0.15958846824663908
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	27097.0
3	4487.0
4	2986.0
5	2457.0
6	2227.0
7	2544.0
8	3024.0
9	3287.0
10	3580.0
11	3792.0
12	4319.0
13	4949.0
14	5507.0
15	5887.0
16	5926.0
17	5898.0
18	5890.0
19	5516.0
20	5623.0
21	5599.0
22	6520.0
23	9016.0
24	14418.0
25	20427.0
26	20941.0
27	17359.0
28	18588.0
29	23595.0
30	28862.0
31	35321.0
32	46649.0
33	64646.0
34	91366.0
35	140712.0
36	270550.0
37	580381.0
38	947860.0
39	413436.0
40	885.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.96997390178025	19.86279592734564	13.371192402946697	23.79603776792741
2	15.160359746480243	20.397678599100107	40.70888707848878	23.733074575930875
3	18.04732455543859	25.874884758229143	29.900768837856006	26.177021848476258
4	11.919484462321563	16.878811805468903	39.338583921149606	31.86311981105993
5	12.602655453431694	38.605965594803045	35.79748366338624	12.993895288379026
6	30.446783114056853	38.49776856535455	18.18930225212067	12.866146068467934
7	27.200569687880645	32.826971282894895	22.67968544172125	17.292773587503206
8	25.046825837238977	36.75129330166882	20.47613489754926	17.72574596354294
9	25.12797896843133	17.094439724867495	21.090102295811125	36.68747901089005
10-11	23.68577438896217	27.13811912579554	29.078060160310777	20.098046324931516
12-13	24.33774646405858	24.585715336556767	29.08705276449824	21.98948543488642
14-15	21.732288665154563	26.5176370314496	26.084851420291283	25.665222883104555
16-17	21.446920650585383	29.85772376191052	26.582885614978757	22.112469972525343
18-19	21.311323594909965	28.941432645891574	28.43523871578058	21.31200504341788
20-21	22.661979243842907	28.119731629375107	27.896293303734932	21.32199582304705
22-23	22.25783872770217	27.846716080927937	27.941136900753655	21.95430829061624
24-25	22.083980821138898	28.048865940361985	27.884399814844553	21.982753423654568
26-27	21.770829438317453	28.10668505288893	27.88822052843149	22.23426498036213
28-29	21.735221044601364	28.08984817899185	28.04945064065568	22.125480135751104
30-31	21.99847180536818	28.371197183061675	27.777095357353094	21.853235654217045
32-33	21.71239282276886	28.099166766268883	28.21781572680614	21.970624684156117
34-35	21.89590115470471	27.916307321143773	28.185868880002126	22.00192264414939
36-37	22.21877455805546	28.177456399569365	27.650977115779668	21.952791926595502
38-39	21.9501324194834	28.324560695437285	27.857414669891277	21.867892215188036
40-41	21.708956774130765	28.120078012760676	28.151980570538633	22.018984642569926
42-43	21.78688347587994	27.932792917640242	28.11533084475156	22.164992761728254
44-45	21.811963046449115	28.131133460965813	28.046765341317876	22.0101381512672
46-47	21.918242839522133	28.2755801236246	27.961329750619345	21.84484728623392
48-49	21.901378492855965	28.102945402738115	27.88166677003252	22.114009334373396
50-51	21.836407243208793	28.064167053351298	27.903576460130488	22.195849243309425
52-53	21.78238527359297	28.064735867506503	28.04274004059881	22.110138818301717
54-55	21.913495269108747	28.374725720115723	27.858310855194198	21.853468155581336
56-57	21.838981262394952	28.159778368806716	27.80585398168866	22.195386387109668
58-59	21.699475043836607	27.807180765804635	28.17642677963004	22.316917410728713
60-61	22.02504511649927	28.19439664434572	27.754552267879323	22.026005971275683
62-63	21.754027002984568	28.176652152614874	27.85055767641002	22.21876316799054
64-65	21.834012997919167	27.986353250536716	27.89156183445324	22.28807191709088
66-67	21.669833067355675	28.00752598850662	27.841161112581485	22.481479831556218
68-69	22.06592585695176	28.170661678165132	27.714205402179594	22.049207062703516
70-71	22.019994958197238	27.946821035044024	27.833791014654945	22.199392992103796
72-73	21.82430038594336	28.07245295201598	27.907834796955218	22.195411865085436
74-75	21.915973403865035	28.09751333824819	27.759995387953474	22.2265178699333
76-77	21.94219209657816	28.105353101933307	27.68253888390114	22.26991591758739
78-79	22.045012868506436	28.23618653359543	27.711680466913997	22.007120130984134
80-81	21.958553136833835	28.084475822371985	27.800236615600276	22.156734425193903
82-83	21.810476649850745	28.09701162043759	27.899584548872763	22.1929271808389
84-85	21.86233099107529	28.017196047732167	27.798891215501598	22.321581745690942
86-87	22.043341835945874	28.112149232292875	27.673252304326795	22.171256627434456
88-89	21.839475598518693	28.060758836937218	27.75240213299533	22.347363431548768
90-91	21.795138379278132	27.98582591218007	27.819881513484628	22.399154195057175
92-93	21.994184637325503	28.10147549492364	27.714493569020217	22.189846298730643
94-95	21.730951562916015	27.85852431344258	27.924093589553202	22.486430534088203
96-97	21.82381727136514	27.906143008227723	27.84800872506315	22.422030995343984
98-99	21.805863893740586	28.01103111159379	27.751807369925512	22.43129762474011
100	21.884011973964324	27.893031460652836	27.641904376289244	22.581052189093597
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	409.0
1	392.0
2	385.5
3	365.0
4	304.0
5	268.0
6	241.5
7	222.0
8	227.0
9	240.5
10	235.0
11	219.5
12	235.5
13	263.5
14	305.5
15	375.5
16	452.0
17	599.0
18	851.0
19	1178.0
20	1715.0
21	2608.0
22	4015.5
23	6025.5
24	8650.5
25	12159.5
26	16716.5
27	22586.5
28	29972.0
29	38047.5
30	45811.5
31	54124.0
32	62932.0
33	70717.0
34	78276.0
35	85156.0
36	90867.0
37	96757.5
38	101567.5
39	103565.0
40	105030.5
41	106476.5
42	104952.5
43	103486.5
44	102452.0
45	100341.0
46	99216.5
47	96790.5
48	93359.5
49	91453.5
50	88407.0
51	84695.5
52	80390.5
53	75853.0
54	72597.0
55	68975.0
56	62995.5
57	56126.5
58	50746.0
59	46504.5
60	42247.5
61	37041.5
62	31766.0
63	26793.0
64	22203.5
65	17864.5
66	13945.0
67	10371.0
68	7837.5
69	6027.5
70	4143.0
71	2836.5
72	2182.0
73	1616.5
74	1273.0
75	982.5
76	675.0
77	481.0
78	340.0
79	241.5
80	167.5
81	100.0
82	61.5
83	46.5
84	37.5
85	31.5
86	27.5
87	20.5
88	16.0
89	14.5
90	12.5
91	10.5
92	8.0
93	5.0
94	2.0
95	2.0
96	2.0
97	2.5
98	3.0
99	2.5
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3298945155124144
2	0.11655667271228703
3	0.06414809685244575
4	0.03892210233857547
5	0.04269551980048405
6	0.010167263716809212
7	0.007302261569804554
8	0.01603702421311144
9	0.018308062500371227
10-11	0.02174955898183414
12-13	0.016683396648716146
14-15	0.019164069239415302
16-17	0.020299588383045197
18-19	0.020107423604892444
20-21	0.024841664957564777
22-23	0.01642135376941694
24-25	0.026116940303487584
26-27	0.019705624523300328
28-29	0.019792972149733398
30-31	0.020282118857758585
32-33	0.026029592677054514
34-35	0.0154779994039398
36-37	0.009992568463943075
38-39	0.013556351622412283
40-41	0.010044977039802916
42-43	0.00894439694674625
44-45	0.009223909351332068
46-47	0.008280554985854926
48-49	0.004349911796366828
50-51	0.006673358659486458
52-53	0.007704060651396671
54-55	0.0030396973998707954
56-57	0.002620428792992065
58-59	0.0027427154699983616
60-61	0.0032143926527369335
62-63	0.00255055069184561
64-65	0.0018692392056676732
66-67	0.0015547877505086253
68-69	0.0027427154699983616
70-71	0.001939117306814128
72-73	0.0017120134780881492
74-75	0.0019915258826739696
76-77	0.0039655822400613255
78-79	0.0035288441078959805
80-81	0.0035288441078959805
82-83	0.002008995407960583
84-85	0.0025854897424188376
86-87	0.0022885078125464034
88-89	0.6012137127388127
90-91	0.6114159155061952
92-93	0.6086557305109103
94-95	0.5955186474953766
96-97	0.5936144692391357
98-99	0.5905223632634051
100	0.5923566634184996
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2862127.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.923476702837874
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.30667685762062	47.25174726663848
2	13.935927096763862	17.259221138178084
3	4.510823259715707	8.379775771008745
4	1.9386159850226687	4.801833671372013
5	0.9807970971828104	3.0367183098805386
6	0.558359651168543	2.0745342510563978
7	0.366086649649447	1.5868550684551255
8	0.2609424621242583	1.2926771579306133
9	0.18443780450850433	1.0278927081544442
>10	0.89557328384984	9.804076354123104
>50	0.04728892878907411	1.9670149872918583
>100	0.014358118370218123	1.472702959915921
>500	1.1280523444152609E-4	0.04495035599472258
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	3.493905057322753E-5	0.0
9	0.0	0.0	0.0	3.493905057322753E-5	0.0
10-11	3.493905057322753E-5	0.0	0.0	3.493905057322753E-5	0.0
12-13	3.493905057322753E-5	0.0	0.0	3.493905057322753E-5	0.0
14-15	3.493905057322753E-5	0.0	0.0	3.493905057322753E-5	0.0
16-17	3.493905057322753E-5	0.0	0.0	3.493905057322753E-5	3.493905057322753E-5
18-19	3.493905057322753E-5	0.0	0.0	3.493905057322753E-5	3.493905057322753E-5
20-21	3.493905057322753E-5	0.0	0.0	6.987810114645506E-5	3.493905057322753E-5
22-23	3.493905057322753E-5	0.0	0.0	1.2228667700629636E-4	3.493905057322753E-5
24-25	3.493905057322753E-5	0.0	0.0	2.096343034393652E-4	3.493905057322753E-5
26-27	3.493905057322753E-5	0.0	0.0	3.319209804456616E-4	3.493905057322753E-5
28-29	3.493905057322753E-5	0.0	0.0	0.00106564104248344	3.493905057322753E-5
30-31	3.493905057322753E-5	0.0	0.0	0.00538061378827704	3.493905057322753E-5
32-33	3.493905057322753E-5	0.0	0.0	0.014866566018908315	3.493905057322753E-5
34-35	5.2408575859841295E-5	0.0	0.0	0.028667490995333188	3.493905057322753E-5
36-37	6.987810114645506E-5	0.0	0.0	0.05237363680926807	3.493905057322753E-5
38-39	6.987810114645506E-5	0.0	0.0	0.09328726503051751	3.493905057322753E-5
40-41	6.987810114645506E-5	0.0	0.0	0.142114588206603	3.493905057322753E-5
42-43	6.987810114645506E-5	0.0	0.0	0.18963169698619245	3.493905057322753E-5
44-45	6.987810114645506E-5	0.0	0.0	0.24929012583997845	3.493905057322753E-5
46-47	6.987810114645506E-5	1.7469525286613765E-5	0.0	0.32107240524267444	3.493905057322753E-5
48-49	6.987810114645506E-5	3.493905057322753E-5	0.0	0.39980755570944265	3.493905057322753E-5
50-51	6.987810114645506E-5	3.493905057322753E-5	0.0	0.4863690535046139	3.493905057322753E-5
52-53	6.987810114645506E-5	3.493905057322753E-5	0.0	0.5738040275641159	3.493905057322753E-5
54-55	6.987810114645506E-5	3.493905057322753E-5	0.0	0.6656762610464175	3.493905057322753E-5
56-57	6.987810114645506E-5	3.493905057322753E-5	0.0	0.7742842997532954	3.493905057322753E-5
58-59	6.987810114645506E-5	3.493905057322753E-5	0.0	0.8995582655836027	3.493905057322753E-5
60-61	6.987810114645506E-5	3.493905057322753E-5	0.0	1.0303176623539068	3.493905057322753E-5
62-63	6.987810114645506E-5	3.493905057322753E-5	0.0	1.1655492575975839	3.493905057322753E-5
64-65	6.987810114645506E-5	3.493905057322753E-5	0.0	1.3076463762789003	3.493905057322753E-5
66-67	6.987810114645506E-5	3.493905057322753E-5	0.0	1.4692045461295045	3.493905057322753E-5
68-69	6.987810114645506E-5	3.493905057322753E-5	0.0	1.6548357218250622	3.493905057322753E-5
70-71	6.987810114645506E-5	6.987810114645506E-5	0.0	1.8556304454694008	3.493905057322753E-5
72-73	6.987810114645506E-5	6.987810114645506E-5	0.0	2.061770843851443	3.493905057322753E-5
74-75	6.987810114645506E-5	6.987810114645506E-5	0.0	2.2750737476009975	3.493905057322753E-5
76-77	6.987810114645506E-5	6.987810114645506E-5	0.0	2.4991029398765323	3.493905057322753E-5
78-79	6.987810114645506E-5	6.987810114645506E-5	0.0	2.7520267269761263	3.493905057322753E-5
80-81	6.987810114645506E-5	6.987810114645506E-5	0.0	3.034910749942263	3.493905057322753E-5
82-83	6.987810114645506E-5	6.987810114645506E-5	0.0	3.327665054695337	3.493905057322753E-5
84-85	6.987810114645506E-5	6.987810114645506E-5	0.0	3.6194410660323597	3.493905057322753E-5
86-87	6.987810114645506E-5	6.987810114645506E-5	0.0	3.9297522436984798	3.493905057322753E-5
88	6.987810114645506E-5	6.987810114645506E-5	0.0	4.187794601707052	3.493905057322753E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	4230	0.0	35.420696	1
GGTATCA	2545	0.0	34.434635	1
TCAACGC	4270	0.0	34.103905	4
ATCAACG	4275	0.0	33.85135	3
CAACGCA	4525	0.0	32.181465	5
AACGCAG	4775	0.0	30.48485	6
TATCAAC	5145	0.0	28.415092	2
GTACATG	9775	0.0	27.715416	1
TACATGG	9650	0.0	27.425537	2
ACGCAGA	5565	0.0	25.904127	7
ACATGGG	9985	0.0	25.551311	3
CGCAGAG	5795	0.0	24.551891	8
CATGGGG	6335	0.0	20.836752	4
GCAGAGT	6870	0.0	20.163273	9
GAGTACA	6915	0.0	20.10019	1
CATGGGT	2675	0.0	19.843805	4
TACACCG	380	1.2732926E-11	18.54259	5
AGTACAT	7175	0.0	18.213648	2
TATACTG	1795	0.0	17.795376	5
CGTATAC	215	2.6316484E-5	17.482878	3
>>END_MODULE