FastQCFastQC Report
Wed 25 May 2016
SRR1033123_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033123_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2862127
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62510.2184040051332453No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49040.17134110401110783No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42250.053.6832351
GTATCAA80600.044.8378221
TCAACGC95300.037.5918054
ATCAACG95950.037.435143
CAACGCA99400.036.088535
AACGCAG102500.034.937776
TATCAAC105650.034.2206232
ACGCAGA121050.029.5061747
CGCAGAG123500.028.8056628
GTACATG95900.027.7235091
TACATGG94450.026.8298282
GCAGAGT140200.024.9722219
ACATGGG101550.024.583613
GAGTACT89850.021.54904712-13
GTGGTAT21700.021.251261
TATACTG16850.021.2053175
CAGAGTA135450.020.73040810-11
AGAGTAC126750.020.33656510-11
CATGGGG62000.020.1707024
TAGTACG3303.274181E-1119.945514
Produced by FastQC (version 0.11.5)