##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033123_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2862127 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.76760604962673 34.0 31.0 34.0 31.0 34.0 2 32.855982980489685 34.0 31.0 34.0 31.0 34.0 3 32.943724370022714 34.0 31.0 34.0 31.0 34.0 4 36.37090247917021 37.0 37.0 37.0 35.0 37.0 5 36.312158405269926 37.0 37.0 37.0 35.0 37.0 6 36.397517300944365 37.0 37.0 37.0 35.0 37.0 7 36.37031131043451 37.0 37.0 37.0 35.0 37.0 8 36.36855981582928 37.0 37.0 37.0 35.0 37.0 9 38.190352140209015 39.0 39.0 39.0 37.0 39.0 10-11 38.16805595978096 39.0 39.0 39.0 37.0 39.0 12-13 38.135060044505366 39.0 39.0 39.0 37.0 39.0 14-15 39.67177137841892 41.0 40.0 41.0 37.0 41.0 16-17 39.68416653069553 41.0 40.0 41.0 37.0 41.0 18-19 39.704135246269644 41.0 40.0 41.0 37.0 41.0 20-21 39.69941236010841 41.0 40.0 41.0 37.0 41.0 22-23 39.626933046646776 41.0 40.0 41.0 37.0 41.0 24-25 39.57951621294234 41.0 40.0 41.0 37.0 41.0 26-27 39.47866324590069 41.0 39.5 41.0 36.5 41.0 28-29 39.39220377013319 41.0 39.0 41.0 36.0 41.0 30-31 39.29162612281006 41.0 39.0 41.0 36.0 41.0 32-33 39.315245969169084 41.0 39.0 41.0 35.5 41.0 34-35 39.253041357004776 41.0 39.0 41.0 35.0 41.0 36-37 39.16436954055498 41.0 39.0 41.0 35.0 41.0 38-39 39.07291605159379 41.0 39.0 41.0 35.0 41.0 40-41 38.95834182061104 41.0 39.0 41.0 35.0 41.0 42-43 38.84220511528664 41.0 39.0 41.0 35.0 41.0 44-45 38.69146320201724 41.0 39.0 41.0 35.0 41.0 46-47 38.464407065095294 41.0 39.0 41.0 35.0 41.0 48-49 38.26209825769436 41.0 38.0 41.0 34.5 41.0 50-51 38.12689688472943 41.0 38.0 41.0 34.0 41.0 52-53 37.99348561402062 40.0 38.0 41.0 34.0 41.0 54-55 37.85793974201704 40.0 37.5 41.0 34.0 41.0 56-57 37.695081140704104 40.0 37.0 41.0 34.0 41.0 58-59 37.505273001512506 40.0 37.0 41.0 33.0 41.0 60-61 37.315185349916334 40.0 36.0 41.0 33.0 41.0 62-63 37.08100671283979 39.5 36.0 41.0 33.0 41.0 64-65 36.83084538177376 39.0 35.0 41.0 33.0 41.0 66-67 36.5476516241243 39.0 35.0 41.0 32.0 41.0 68-69 36.22580863812123 38.0 35.0 40.5 32.0 41.0 70-71 35.891708509091316 37.0 35.0 40.0 31.5 41.0 72-73 35.5365647645964 37.0 35.0 39.5 31.0 41.0 74-75 35.202783978488725 36.0 35.0 39.0 31.0 41.0 76-77 34.86442006242211 36.0 35.0 39.0 31.0 40.5 78-79 34.52399596523844 35.0 35.0 37.5 31.0 39.5 80-81 34.18638358814965 35.0 35.0 37.0 31.0 39.0 82-83 33.865288297828855 35.0 34.5 37.0 30.0 39.0 84-85 33.59233936858847 35.0 34.0 36.0 30.0 37.5 86-87 33.3639415721245 35.0 34.0 36.0 30.0 37.0 88-89 33.14829705320554 35.0 34.0 35.5 30.0 37.0 90-91 32.94925731807149 35.0 34.0 35.0 30.0 36.0 92-93 32.791101862356214 35.0 34.0 35.0 29.0 36.0 94-95 32.642197743146966 35.0 34.0 35.0 29.0 36.0 96-97 32.523231498811896 35.0 34.0 35.0 29.0 36.0 98-99 32.41996546624242 35.0 34.0 35.0 29.0 35.5 100 32.32253635146169 35.0 34.0 35.0 29.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.11025643271731411 1101 2 0.12231982189508983 1101 3 0.13756442476339004 1101 4 0.07911902054282649 1101 5 0.08322595450213299 1101 6 0.06333109544983273 1101 7 0.08579300397131107 1101 8 0.11464232498780547 1101 9 0.16394850234628677 1101 10-11 0.13546751785965938 1101 12-13 0.17813694765278143 1101 14-15 0.19436617916058196 1101 16-17 0.19042013828079263 1101 18-19 0.25507198840351464 1101 20-21 0.21158897247453723 1101 22-23 0.2521862679991358 1101 24-25 0.23922615789518886 1101 26-27 0.23262235085878302 1101 28-29 0.23465888858859074 1101 30-31 0.2211421760923713 1101 32-33 0.1532409101317782 1101 34-35 0.1859258627873075 1101 36-37 0.20900715721035823 1101 38-39 0.1565827012280181 1101 40-41 0.22300188813348854 1101 42-43 0.1353286557150497 1101 44-45 0.16618587176590438 1101 46-47 0.22502345758645248 1101 48-49 0.22593255964994086 1101 50-51 0.2451182675009278 1101 52-53 0.2792603836186345 1101 54-55 0.3161180437907234 1101 56-57 0.32467114949269416 1101 58-59 0.3044076135998921 1101 60-61 0.27927788400486975 1101 62-63 0.2568485108531746 1101 64-65 0.2739788359735229 1101 66-67 0.278808931151616 1101 68-69 0.2374347133905914 1101 70-71 0.2703470481767525 1101 72-73 0.31284445717471954 1101 74-75 0.24216624219824467 1101 76-77 0.277778783331577 1101 78-79 0.22215530871643097 1101 80-81 0.14024208623971646 1101 82-83 0.24941245325785388 1101 84-85 0.2767207058092893 1101 86-87 0.14821817407670324 1101 88-89 0.14860373434297713 1101 90-91 0.2127444732892414 1101 92-93 0.2066965003829253 1101 94-95 0.21951750646562118 1101 96-97 0.25079888662199323 1101 98-99 0.2500259431498719 1101 100 0.2524053930352821 1102 1 0.11823636165119211 1102 2 0.15979845605862408 1102 3 0.13374891736004457 1102 4 0.10020680800722914 1102 5 0.033261528797005724 1102 6 0.05352658270686561 1102 7 0.09997272979283878 1102 8 0.13320009849306302 1102 9 0.2187244126346357 1102 10-11 0.17362748802930383 1102 12-13 0.20254212508489644 1102 14-15 0.205968540232071 1102 16-17 0.1809320705512505 1102 18-19 0.22078215620955888 1102 20-21 0.19486525293655177 1102 22-23 0.1957462512079644 1102 24-25 0.18233208889419927 1102 26-27 0.20913532781008826 1102 28-29 0.19049644870477067 1102 30-31 0.15550856839502103 1102 32-33 0.16784041520971016 1102 34-35 0.24055128131032433 1102 36-37 0.23725786309146457 1102 38-39 0.23650548916012326 1102 40-41 0.27029508500377375 1102 42-43 0.21649551320218308 1102 44-45 0.2509674950721319 1102 46-47 0.2630672089441646 1102 48-49 0.25950059829127525 1102 50-51 0.22620700584650422 1102 52-53 0.28579994658951335 1102 54-55 0.2756799529849232 1102 56-57 0.3048524920699336 1102 58-59 0.26378127924171224 1102 60-61 0.30949522606776014 1102 62-63 0.20872954195884574 1102 64-65 0.19986228577047171 1102 66-67 0.17179311585861257 1102 68-69 0.223758065563743 1102 70-71 0.2561450320509451 1102 72-73 0.24046332956362448 1102 74-75 0.19308808156838353 1102 76-77 0.19454807558993537 1102 78-79 0.2561443506482277 1102 80-81 0.18981250182633858 1102 82-83 0.14283368254365314 1102 84-85 0.18224209148798565 1102 86-87 0.20930694334525413 1102 88-89 0.24382024792808465 1102 90-91 0.16072044923735973 1102 92-93 0.20086043487675198 1102 94-95 0.14253098295166922 1102 96-97 0.08897327462429416 1102 98-99 0.1316552793549235 1102 100 0.17546660684929094 1103 1 0.12719994197237838 1103 2 0.1386120148545089 1103 3 0.10047521167551565 1103 4 0.08345140144214724 1103 5 0.04163324075498309 1103 6 0.09587568607565089 1103 7 0.08026145824253916 1103 8 0.11097054595249745 1103 9 0.15492425640076135 1103 10-11 0.11939088273739884 1103 12-13 0.09434994797910434 1103 14-15 0.15105165573574197 1103 16-17 0.1264598168409421 1103 18-19 0.09377039048984415 1103 20-21 0.14334203472233042 1103 22-23 0.19373096209271523 1103 24-25 0.15216732188098092 1103 26-27 0.19100973946512312 1103 28-29 0.21846817362635562 1103 30-31 0.19413709025391768 1103 32-33 0.20214335595048283 1103 34-35 0.16622719274599973 1103 36-37 0.18204252991980496 1103 38-39 0.19198724691168678 1103 40-41 0.28055782041781896 1103 42-43 0.17300551262975006 1103 44-45 0.1829111701642745 1103 46-47 0.21938247480189688 1103 48-49 0.22302153396605462 1103 50-51 0.22843475416021164 1103 52-53 0.21726661290849592 1103 54-55 0.20715720501206647 1103 56-57 0.19415916978299208 1103 58-59 0.25542425068538677 1103 60-61 0.24398741816116853 1103 62-63 0.22320225386577874 1103 64-65 0.2419137786285006 1103 66-67 0.20641598712236942 1103 68-69 0.26432439287930265 1103 70-71 0.2850547252193749 1103 72-73 0.3009343892308891 1103 74-75 0.22682581999671214 1103 76-77 0.22220067710998137 1103 78-79 0.1806216246539165 1103 80-81 0.18147133631068613 1103 82-83 0.17032262466943848 1103 84-85 0.19116016257701318 1103 86-87 0.26963228654881277 1103 88-89 0.2427819482771696 1103 90-91 0.24234023270281568 1103 92-93 0.20001553394097726 1103 94-95 0.2572261996147347 1103 96-97 0.2929845502350332 1103 98-99 0.2653285598230397 1103 100 0.2775467326509684 1104 1 0.018561853662539818 1104 2 0.06427269140596081 1104 3 0.06388782407412918 1104 4 0.020528680503190344 1104 5 -0.02978437271467982 1104 6 0.0013556906285074888 1104 7 0.03153000105316295 1104 8 0.028707381025320444 1104 9 0.04913028865109226 1104 10-11 0.03612435760514643 1104 12-13 0.037188373935471475 1104 14-15 0.11665603665701951 1104 16-17 0.12135384984349429 1104 18-19 0.08773282024403528 1104 20-21 0.08823675701584222 1104 22-23 0.07236043249809399 1104 24-25 -0.0091528329160937 1104 26-27 -0.04545912543880348 1104 28-29 -0.07013503656072118 1104 30-31 0.02102740886187604 1104 32-33 -0.026863315679079847 1104 34-35 -0.05767996294255795 1104 36-37 -0.07176120738044744 1104 38-39 -0.029671052823132982 1104 40-41 7.842507319111292E-4 1104 42-43 -0.008102506687372113 1104 44-45 0.04417077340133346 1104 46-47 0.05876557352885925 1104 48-49 0.02321269732659914 1104 50-51 -0.0014273452291817534 1104 52-53 0.04526085750569564 1104 54-55 0.044193798232136317 1104 56-57 0.04794164510814625 1104 58-59 0.036834179444511506 1104 60-61 0.067421207699077 1104 62-63 0.07311842548014624 1104 64-65 0.12548016300400633 1104 66-67 0.09313066378835799 1104 68-69 0.16431269260705506 1104 70-71 0.11571846999932944 1104 72-73 0.13622212755765872 1104 74-75 0.13070870203888063 1104 76-77 -0.016184328301839912 1104 78-79 0.05705976838981286 1104 80-81 0.039105710932908266 1104 82-83 0.005022256411322701 1104 84-85 0.060078760495564154 1104 86-87 0.020272885291689136 1104 88-89 0.05042090049833803 1104 90-91 0.049098836644716926 1104 92-93 0.07168086797844353 1104 94-95 0.0423048353417812 1104 96-97 0.040218991170576146 1104 98-99 0.05492293506738832 1104 100 0.09552566791911943 1105 1 0.0591296705195532 1105 2 0.061309956923786046 1105 3 0.0648760786582514 1105 4 0.04290611934774802 1105 5 0.037017172777495944 1105 6 0.042057439318277545 1105 7 -0.017746767049956702 1105 8 -0.032280981786549034 1105 9 -0.044509031570449054 1105 10-11 -0.04975943014943596 1105 12-13 0.020126608362176057 1105 14-15 -0.015166369732746432 1105 16-17 0.05772405366338518 1105 18-19 -1.437108403266052E-4 1105 20-21 0.07418541377425214 1105 22-23 0.07146789182475288 1105 24-25 0.03840886366201346 1105 26-27 0.04779429523431844 1105 28-29 0.027615246860676734 1105 30-31 0.09121512988322422 1105 32-33 0.05938878461557806 1105 34-35 0.12134935595319973 1105 36-37 0.11098370523917822 1105 38-39 0.07632990499240577 1105 40-41 0.09732576554152672 1105 42-43 0.13443241705265763 1105 44-45 0.13839993482571344 1105 46-47 0.13754962130059312 1105 48-49 0.05940405480013311 1105 50-51 0.07152356537199012 1105 52-53 0.10477597015724882 1105 54-55 0.09574740749209809 1105 56-57 0.09393674509704653 1105 58-59 0.09219233210515654 1105 60-61 0.07791084353977595 1105 62-63 0.07269802833843642 1105 64-65 0.02611762441405574 1105 66-67 0.029184977881499208 1105 68-69 0.00220714891145235 1105 70-71 0.10099746759396311 1105 72-73 0.019386219756867717 1105 74-75 -0.07984634394437506 1105 76-77 -0.04002385632065142 1105 78-79 0.0029797177392296703 1105 80-81 0.07543911731996644 1105 82-83 0.06412204509665287 1105 84-85 0.06264477265495572 1105 86-87 0.08181963561840178 1105 88-89 0.02081892155405285 1105 90-91 0.08187966734676166 1105 92-93 0.1018128246548784 1105 94-95 0.08110076110837383 1105 96-97 0.11318173918121488 1105 98-99 0.08322854856988471 1105 100 0.10845347189702892 1106 1 0.031691972464642504 1106 2 0.011231132131364063 1106 3 0.009137897099634529 1106 4 0.04370709849878551 1106 5 0.026880341468860536 1106 6 0.015041177515506376 1106 7 0.004775589310796136 1106 8 0.07252819018098933 1106 9 0.005878770303809233 1106 10-11 0.00998612268070076 1106 12-13 0.03578242650018382 1106 14-15 0.08511143760614459 1106 16-17 0.10133239564198249 1106 18-19 0.06563434883800312 1106 20-21 0.005917746367870791 1106 22-23 0.06294773589878844 1106 24-25 0.08587517542125056 1106 26-27 -0.020814694187002658 1106 28-29 -0.06441607152206075 1106 30-31 -0.005900871832551502 1106 32-33 0.04111484952829869 1106 34-35 0.06115548983651564 1106 36-37 0.0620336614851098 1106 38-39 0.04122675702790701 1106 40-41 0.019696674709983597 1106 42-43 0.06689061999973234 1106 44-45 0.04814328961496983 1106 46-47 0.05483135856245269 1106 48-49 -0.06695163043257679 1106 50-51 -0.10213205515997004 1106 52-53 -0.05928027814390191 1106 54-55 -0.046002942507826106 1106 56-57 -0.056999865145250794 1106 58-59 -0.1165298477159098 1106 60-61 -0.10208286048770532 1106 62-63 -0.13880829124668992 1106 64-65 -0.09819780010924717 1106 66-67 -0.08185667368724125 1106 68-69 -0.14656702981016423 1106 70-71 -0.13394102861093415 1106 72-73 -0.05219711613359124 1106 74-75 -0.029858188337890113 1106 76-77 -0.16207105500460983 1106 78-79 -0.04964467233163106 1106 80-81 -0.09595730892673515 1106 82-83 -0.06715570108881508 1106 84-85 -0.15544318314744743 1106 86-87 -0.12359658735957169 1106 88-89 -0.20557602373540362 1106 90-91 -0.18000637468021807 1106 92-93 -0.1799348833434422 1106 94-95 -0.20486343128244755 1106 96-97 -0.1868921889855102 1106 98-99 -0.16962338225668105 1106 100 -0.0982541544995712 1107 1 0.03725418901492361 1107 2 -0.008796040805449934 1107 3 -0.002746323583942001 1107 4 0.012543674944048178 1107 5 -0.13471890372266415 1107 6 -0.12389821448215343 1107 7 -0.0785964704646247 1107 8 -0.030456536544434698 1107 9 -0.06380666103977717 1107 10-11 -0.02630321468326713 1107 12-13 0.01951361800216489 1107 14-15 0.04838298755810655 1107 16-17 0.02547131355236587 1107 18-19 0.05230952026553126 1107 20-21 -0.047589635011043185 1107 22-23 -0.03915658578307557 1107 24-25 0.008443781624386304 1107 26-27 8.048235616442412E-4 1107 28-29 0.033224695068206245 1107 30-31 0.01764240643709769 1107 32-33 -0.0307338177603782 1107 34-35 0.031149605238091738 1107 36-37 -0.004297433207760548 1107 38-39 0.039172058414514765 1107 40-41 0.014581212838344015 1107 42-43 -0.004154298541330093 1107 44-45 0.006943038474346963 1107 46-47 -0.06153484203532855 1107 48-49 -0.047029449572256965 1107 50-51 -0.017170985738722777 1107 52-53 0.012772648367111117 1107 54-55 -0.025066030823232666 1107 56-57 -0.08293114854024708 1107 58-59 -0.08509196888569903 1107 60-61 -0.07055537861473482 1107 62-63 -0.03547394983535668 1107 64-65 -0.0012097806558273305 1107 66-67 -0.010631419831213407 1107 68-69 0.01014309835895233 1107 70-71 -0.030625884100025758 1107 72-73 0.011870766417303003 1107 74-75 0.07135062813932791 1107 76-77 0.012336409422566419 1107 78-79 -1.8261097424243644E-4 1107 80-81 0.014520012679703598 1107 82-83 0.06399626210019704 1107 84-85 0.006304857718255619 1107 86-87 -0.03839845854001567 1107 88-89 -0.008351112955040207 1107 90-91 0.011097088367094443 1107 92-93 0.019492103622411605 1107 94-95 -0.018961172760548095 1107 96-97 -0.018909882487633922 1107 98-99 -0.04236647279063277 1107 100 -0.018761234190392884 1108 1 0.014609331491264754 1108 2 -0.0036040595751813953 1108 3 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-0.15519472064399764 2214 30-31 -0.16374651583661404 2214 32-33 -0.16784938580258313 2214 34-35 -0.2251873691074664 2214 36-37 -0.23126614146180913 2214 38-39 -0.2491245271061615 2214 40-41 -0.22234236702576737 2214 42-43 -0.2449348380072749 2214 44-45 -0.22710849899181085 2214 46-47 -0.18470745906139285 2214 48-49 -0.26085776655283865 2214 50-51 -0.19892401546600524 2214 52-53 -0.276359136662208 2214 54-55 -0.3087828856899648 2214 56-57 -0.3238631580152642 2214 58-59 -0.31391497565439863 2214 60-61 -0.347111321164256 2214 62-63 -0.3637001138343692 2214 64-65 -0.2840351750572836 2214 66-67 -0.31696235219862956 2214 68-69 -0.3051309896948595 2214 70-71 -0.22598654206537816 2214 72-73 -0.2830919480822729 2214 74-75 -0.22683606347156626 2214 76-77 -0.2427522399013995 2214 78-79 -0.3690143727702164 2214 80-81 -0.2727799034522249 2214 82-83 -0.28352654554957724 2214 84-85 -0.3116419295100954 2214 86-87 -0.27745927719487895 2214 88-89 -0.44165907589906794 2214 90-91 -0.3417909538245141 2214 92-93 -0.37727490536382646 2214 94-95 -0.4023014623904544 2214 96-97 -0.49773181450383674 2214 98-99 -0.5761819245165825 2214 100 -0.7022703627642493 2215 1 -0.052455254329743184 2215 2 -0.09409584314046526 2215 3 -0.08021314186685657 2215 4 -0.06856470653407598 2215 5 -0.08554100910627938 2215 6 -0.10188377666462856 2215 7 -0.08107999730768256 2215 8 -0.11702129711772358 2215 9 -0.1160835858686724 2215 10-11 -0.1311341122614067 2215 12-13 -0.13508435017795506 2215 14-15 -0.19537180227520423 2215 16-17 -0.18239793917744151 2215 18-19 -0.13172444394838578 2215 20-21 -0.17457324099748206 2215 22-23 -0.1652715032703327 2215 24-25 -0.16464163021328915 2215 26-27 -0.16869117310972825 2215 28-29 -0.13558807950359864 2215 30-31 -0.2111367547683045 2215 32-33 -0.18644152087188104 2215 34-35 -0.16243217014983458 2215 36-37 -0.14691174288487474 2215 38-39 -0.14168244231970561 2215 40-41 -0.2622184055044201 2215 42-43 -0.2207399883482779 2215 44-45 -0.12801506988999733 2215 46-47 -0.16247969798239126 2215 48-49 -0.13008011479130488 2215 50-51 -0.1311767577700209 2215 52-53 -0.08224290298589665 2215 54-55 -0.07447135902505408 2215 56-57 -0.19832103152780434 2215 58-59 -0.2458589291107529 2215 60-61 -0.11571596321808642 2215 62-63 -0.18217572981527042 2215 64-65 -0.11731379680955456 2215 66-67 -0.10648656826974445 2215 68-69 -0.05858512454125275 2215 70-71 0.07852906169826923 2215 72-73 0.09003280729582741 2215 74-75 0.03481059181260804 2215 76-77 -0.023051026477283187 2215 78-79 -0.032666617837143974 2215 80-81 -0.04441962901727692 2215 82-83 0.030400216947739978 2215 84-85 0.0037885310549015117 2215 86-87 -0.06127092030841652 2215 88-89 -0.04736788121822144 2215 90-91 -0.08108765883197577 2215 92-93 -0.051693664240730186 2215 94-95 0.008615276157698304 2215 96-97 -0.04418493109309907 2215 98-99 -0.14520653183767962 2215 100 -0.14054125464052447 2216 1 -0.06292425856148753 2216 2 -0.08699897701526993 2216 3 -0.13860057322946062 2216 4 -0.08843765486838606 2216 5 -0.07556775159303442 2216 6 -0.10526850726804327 2216 7 -0.064312093593891 2216 8 -0.058630551940154874 2216 9 -0.11521072700736568 2216 10-11 -0.11469858418841739 2216 12-13 -0.11994485513708497 2216 14-15 -0.2042657502549332 2216 16-17 -0.21812384691420306 2216 18-19 -0.14452670529571776 2216 20-21 -0.14881694557751501 2216 22-23 -0.1532983584267953 2216 24-25 -0.2091872564746069 2216 26-27 -0.23564746720417418 2216 28-29 -0.22544084670478526 2216 30-31 -0.2011108075971464 2216 32-33 -0.1895449085849208 2216 34-35 -0.13655094390575329 2216 36-37 -0.169448335686603 2216 38-39 -0.15636264260668042 2216 40-41 -0.2476024932277312 2216 42-43 -0.2088414043414062 2216 44-45 -0.1308603114173934 2216 46-47 -0.16771088628330233 2216 48-49 -0.2119260422784066 2216 50-51 -0.14423915359196826 2216 52-53 -0.08966883093692957 2216 54-55 -0.05315855808922976 2216 56-57 -0.03615817077762529 2216 58-59 -0.09274576564358483 2216 60-61 -0.1273742956401236 2216 62-63 -0.06217553098636586 2216 64-65 -0.08843423078675983 2216 66-67 -0.058180424788183416 2216 68-69 -0.006420374916544347 2216 70-71 -0.07400385276884691 2216 72-73 -0.10451298465623893 2216 74-75 -0.08679548174746543 2216 76-77 -0.07455698968730928 2216 78-79 -0.09242530583151876 2216 80-81 -0.07211019823127174 2216 82-83 -0.08194871451262031 2216 84-85 -0.052025391607287474 2216 86-87 -0.056392984682588576 2216 88-89 -0.01954575355019017 2216 90-91 -0.1449584598141911 2216 92-93 -0.08702191418191063 2216 94-95 -0.1614639571580767 2216 96-97 -0.11532789901205831 2216 98-99 -0.07815778230575887 2216 100 -0.10218450920447708 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 22.0 3 8.0 4 13.0 5 27.0 6 55.0 7 170.0 8 371.0 9 617.0 10 1174.0 11 1852.0 12 3004.0 13 4657.0 14 5529.0 15 7153.0 16 9106.0 17 13411.0 18 16366.0 19 8804.0 20 5627.0 21 5282.0 22 5680.0 23 5654.0 24 6520.0 25 7926.0 26 9878.0 27 12315.0 28 16059.0 29 20662.0 30 26599.0 31 34180.0 32 43053.0 33 56626.0 34 79319.0 35 123078.0 36 234633.0 37 549629.0 38 1022915.0 39 522128.0 40 2025.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.02836605864853 19.86690722078511 13.55641825222442 23.54830846834194 2 15.565620440404516 20.435268418669395 40.30602048289086 23.693090658035228 3 18.059436216492138 25.956011036547295 30.222348623942963 25.7622041230176 4 12.061230115707897 16.912003370926666 39.215987025006974 31.81077948835846 5 12.390199912967342 38.19866864499938 35.90736789124063 13.503763550792645 6 30.338504088924235 38.53448595133261 17.78435720070633 13.342652759036827 7 26.894925667677143 33.0341032811882 22.620485169174692 17.450485881959963 8 25.312731362510533 35.92087825776758 20.40008091901076 18.36630946071113 9 25.53964387923773 16.105095560317373 21.27179198349195 37.08346857695295 10-11 23.888205148810275 26.572651192692128 29.046449001482294 20.492694657015303 12-13 24.88163523142055 24.104905198127128 29.336451527133494 21.677008043318832 14-15 21.981394942568897 25.8091627724156 26.98077477398786 25.22866751102765 16-17 21.003156044438278 29.41674146535077 27.325813983795967 22.254288506414984 18-19 20.762346558420695 27.914640999942876 30.193316256269163 21.129696185367266 20-21 22.066543518159744 27.033636173377353 29.95382455076242 20.94599575770048 22-23 21.629693503524138 26.883017873807567 29.959722178063053 21.52756644460524 24-25 21.476036161942613 27.076437961304972 29.883949862427393 21.56357601432502 26-27 21.218504057370964 27.133495802870293 29.958019968030186 21.689980171728553 28-29 21.200210211322478 27.12868664745786 30.038624932823925 21.63247820839573 30-31 21.46382178247929 27.48780131038594 29.681913958278834 21.36646294885594 32-33 21.262883017543846 27.193199349892634 30.083507098950907 21.460410533612617 34-35 21.366586248197052 27.03834258967044 30.04479109546312 21.55028006666939 36-37 21.73408265290021 27.371873046705375 29.45796767716235 21.436076623232065 38-39 21.53444010099268 27.476365989951407 29.56489237506664 21.424301533989272 40-41 21.32059424311906 27.30052692566928 29.74312177028012 21.63575706093154 42-43 21.466301957359484 27.205685774764394 29.56032538668238 21.76768688119374 44-45 21.523282491433644 27.43659323742742 29.361590223258204 21.67853404788073 46-47 21.723050547880725 27.566540854041865 29.076536964695006 21.633871633382405 48-49 21.771555923310125 27.569298751979517 28.730014410450444 21.92913091425992 50-51 21.762372892193852 27.634136374492186 28.557406875837188 22.046083857476777 52-53 21.7007985607074 27.684561794419665 28.62246749301135 21.99217215186158 54-55 21.835053052080035 27.968930107942036 28.430390786300062 21.765626053677867 56-57 21.677238658322278 27.71886590822257 28.49596800328157 22.10792743017358 58-59 21.581154623960373 27.318950613746868 28.845683507029126 22.25421125526363 60-61 21.857845886157218 27.680378602462174 28.48137996334865 21.980395548031957 62-63 21.5727808081071 27.69868524101411 28.563387476291307 22.165146474587484 64-65 21.61585082497273 27.508530646386454 28.645257184226324 22.230361344414497 66-67 21.50839145336384 27.469643446431192 28.56568854744675 22.45627655275822 68-69 21.912068088716303 27.64143119912917 28.380751195935694 22.065749516218833 70-71 21.88821902683854 27.45983302557724 28.428469855334253 22.223478092249962 72-73 21.777552225456045 27.54492315110181 28.449849935886462 22.227674687555684 74-75 21.81751511384429 27.63736277174435 28.27572026381926 22.2694018505921 76-77 21.824893950876223 27.592879676127847 28.190409406743328 22.391816966252602 78-79 21.91957024937765 27.74007075162685 28.20706642791632 22.13329257107918 80-81 21.85518343893431 27.598916953066627 28.29866282241723 22.24723678558183 82-83 21.74553721791849 27.601693991438502 28.323747185648664 22.32902160499435 84-85 21.72002631616645 27.51241300588829 28.248773024461947 22.51878765348331 86-87 21.851632835421963 27.598451848235005 28.13448760491558 22.415427711427448 88-89 21.585593788394746 27.51999256496874 28.241449146290353 22.652964500346158 90-91 21.608884444640104 27.40770322480562 28.277074454444005 22.70633787611028 92-93 21.661830810063776 27.56677596185913 28.161927853293694 22.6094653747834 94-95 21.475374782460737 27.264653175767535 28.371941566534257 22.88803047523747 96-97 21.467932764688637 27.24269398248226 28.340898220099948 22.948475032729156 98-99 21.438636370782987 27.36375779271849 28.18889588058112 23.0087099559174 100 21.558756826653745 27.264303785261802 28.070312742935588 23.10662664514887 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 46.0 1 44.0 2 130.5 3 243.0 4 176.5 5 139.5 6 356.0 7 801.5 8 1411.0 9 1683.5 10 1335.0 11 997.5 12 1057.5 13 1244.5 14 1428.0 15 1642.5 16 1870.5 17 2154.5 18 2547.0 19 2928.5 20 3496.5 21 4373.5 22 5636.0 23 7587.0 24 9991.0 25 13070.5 26 17397.0 27 23021.0 28 29924.0 29 37737.5 30 45442.5 31 53486.5 32 61883.5 33 69535.0 34 77129.5 35 84316.0 36 89905.5 37 94995.5 38 99329.5 39 101879.0 40 103703.5 41 105132.0 42 103995.0 43 102757.0 44 101928.5 45 99852.0 46 98529.0 47 96284.5 48 93386.5 49 91085.5 50 87955.0 51 84550.0 52 80306.5 53 75917.0 54 72330.5 55 68102.5 56 62413.5 57 56142.5 58 51181.5 59 46977.0 60 42416.5 61 37040.5 62 31829.5 63 26820.0 64 21823.5 65 17239.0 66 13428.5 67 10167.0 68 7486.5 69 5616.5 70 3875.0 71 2611.0 72 1955.0 73 1461.5 74 1108.0 75 792.0 76 533.5 77 393.5 78 265.5 79 168.5 80 103.5 81 54.5 82 28.5 83 12.5 84 5.0 85 2.0 86 2.0 87 1.5 88 1.0 89 1.0 90 1.5 91 1.5 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09147043440070968 2 0.0010831105677700534 3 0.0 4 2.096343034393652E-4 5 0.039551005248893566 6 1.7469525286613766E-4 7 0.004017990815921166 8 2.096343034393652E-4 9 2.795124045858203E-4 10-11 3.3192098044566154E-4 12-13 0.0 14-15 6.987810114645507E-5 16-17 0.0 18-19 1.2228667700629636E-4 20-21 0.0 22-23 2.096343034393652E-4 24-25 6.987810114645507E-5 26-27 0.0018168306298078317 28-29 0.008560067390440745 30-31 0.037087802183481025 32-33 0.026326574606926945 34-35 0.02948855868380404 36-37 0.02709523371953795 38-39 0.02555791549431594 40-41 0.031707188395203985 42-43 0.023409163884062445 44-45 0.022430870468012076 46-47 0.016211719465977575 48-49 0.011005800930566674 50-51 0.008228146409995085 52-53 0.005782412869869157 54-55 0.004472198473373125 56-57 0.0029174107228644988 58-59 0.0012228667700629637 60-61 7.686591126110057E-4 62-63 0.0015198486999353977 64-65 0.0032493317033101604 66-67 6.463724356047094E-4 68-69 0.0013102143964960325 70-71 4.192686068787304E-4 72-73 5.93963859744868E-4 74-75 5.590248091716406E-4 76-77 3.493905057322753E-4 78-79 6.987810114645507E-5 80-81 5.764943344582543E-4 82-83 3.8432955630550284E-4 84-85 2.795124045858203E-4 86-87 1.572257275795239E-4 88-89 1.746952528661377E-5 90-91 3.493905057322754E-5 92-93 1.746952528661377E-5 94-95 0.0 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2862127.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.83587928041608 #Duplication Level Percentage of deduplicated Percentage of total 1 74.94037020309368 44.84122944702004 2 14.688601547957846 17.57810778043477 3 4.76498004114944 8.5535031154743 4 2.04111778506044 4.88528309535947 5 1.0786731006117247 3.227167671561765 6 0.6139909210689067 2.2043211979410353 7 0.39198185025218224 1.6418205070262597 8 0.25337499335193536 1.2128732411906107 9 0.1926539003852399 1.037485397371727 >10 0.9633324951813493 10.19930302710916 >50 0.0515119627569373 2.0650991352939774 >100 0.018826772513270817 1.8853259718074347 >500 3.5065597023895185E-4 0.13931920713119383 >1k 1.7532798506878652E-4 0.3105657324925102 >5k 5.844266168959551E-5 0.21859547278579905 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6251 0.2184040051332453 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4904 0.17134110401110783 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 3.493905057322753E-5 0.0 0.0 0.0 6 0.0 3.493905057322753E-5 0.0 0.0 0.0 7 0.0 3.493905057322753E-5 0.0 0.0 0.0 8 0.0 3.493905057322753E-5 0.0 6.987810114645506E-5 0.0 9 0.0 3.493905057322753E-5 0.0 6.987810114645506E-5 0.0 10-11 0.0 3.493905057322753E-5 0.0 6.987810114645506E-5 0.0 12-13 0.0 3.493905057322753E-5 0.0 6.987810114645506E-5 0.0 14-15 0.0 3.493905057322753E-5 0.0 6.987810114645506E-5 0.0 16-17 0.0 3.493905057322753E-5 0.0 6.987810114645506E-5 0.0 18-19 0.0 3.493905057322753E-5 0.0 6.987810114645506E-5 0.0 20-21 0.0 3.493905057322753E-5 0.0 1.0481715171968259E-4 0.0 22-23 0.0 3.493905057322753E-5 0.0 1.572257275795239E-4 0.0 24-25 0.0 5.2408575859841295E-5 0.0 2.445733540125927E-4 0.0 26-27 0.0 6.987810114645506E-5 0.0 3.8432955630550284E-4 0.0 28-29 0.0 6.987810114645506E-5 0.0 0.0011355191436298949 0.0 30-31 0.0 6.987810114645506E-5 0.0 0.005485430939996722 0.0 32-33 0.0 6.987810114645506E-5 0.0 0.01493644412005477 0.0 34-35 0.0 6.987810114645506E-5 0.0 0.028684960520619803 0.0 36-37 0.0 6.987810114645506E-5 0.0 0.05242604538512791 0.0 38-39 0.0 6.987810114645506E-5 0.0 0.09332220408109074 0.0 40-41 0.0 6.987810114645506E-5 0.0 0.1421320577318896 0.0 42-43 0.0 6.987810114645506E-5 0.0 0.18973651413791212 0.0 44-45 0.0 6.987810114645506E-5 0.0 0.24946482109284457 0.0 46-47 0.0 6.987810114645506E-5 0.0 0.3217187776782791 0.0 48-49 0.0 6.987810114645506E-5 0.0 0.40069850149905994 0.0 50-51 0.0 6.987810114645506E-5 0.0 0.4875569812241036 0.0 52-53 0.0 6.987810114645506E-5 0.0 0.575463632466344 0.0 54-55 0.0 6.987810114645506E-5 0.0 0.6678424821819577 0.0 56-57 0.0 6.987810114645506E-5 0.0 0.7773589362037394 0.0 58-59 0.0 6.987810114645506E-5 0.0 0.9033142135202246 0.0 60-61 0.0 6.987810114645506E-5 0.0 1.034702513200847 0.0 62-63 0.0 6.987810114645506E-5 0.0 1.1702310903743962 0.0 64-65 0.0 6.987810114645506E-5 0.0 1.3130095205418906 0.0 66-67 0.0 6.987810114645506E-5 0.0 1.4757032095361247 0.0 68-69 0.0 6.987810114645506E-5 0.0 1.6626096605776053 0.0 70-71 0.0 6.987810114645506E-5 0.0 1.8655880748827707 0.0 72-73 0.0 6.987810114645506E-5 0.0 2.0724447238015644 0.0 74-75 0.0 6.987810114645506E-5 0.0 2.2862542437844304 0.0 76-77 0.0 6.987810114645506E-5 0.0 2.511663528557608 0.0 78-79 0.0 6.987810114645506E-5 0.0 2.7684655502708297 0.0 80-81 0.0 6.987810114645506E-5 0.0 3.055297685951741 0.0 82-83 0.0 6.987810114645506E-5 0.0 3.3527163539563407 0.0 84-85 0.0 6.987810114645506E-5 0.0 3.648510356109285 0.0 86-87 0.0 6.987810114645506E-5 0.0 3.9627521769648935 0.0 88 0.0 6.987810114645506E-5 0.0 4.224550482910088 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4225 0.0 53.683235 1 GTATCAA 8060 0.0 44.837822 1 TCAACGC 9530 0.0 37.591805 4 ATCAACG 9595 0.0 37.43514 3 CAACGCA 9940 0.0 36.08853 5 AACGCAG 10250 0.0 34.93777 6 TATCAAC 10565 0.0 34.220623 2 ACGCAGA 12105 0.0 29.506174 7 CGCAGAG 12350 0.0 28.805662 8 GTACATG 9590 0.0 27.723509 1 TACATGG 9445 0.0 26.829828 2 GCAGAGT 14020 0.0 24.972221 9 ACATGGG 10155 0.0 24.58361 3 GAGTACT 8985 0.0 21.549047 12-13 GTGGTAT 2170 0.0 21.25126 1 TATACTG 1685 0.0 21.205317 5 CAGAGTA 13545 0.0 20.730408 10-11 AGAGTAC 12675 0.0 20.336565 10-11 CATGGGG 6200 0.0 20.170702 4 TAGTACG 330 3.274181E-11 19.94551 4 >>END_MODULE