FastQCFastQC Report
Wed 25 May 2016
SRR1033122_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033122_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1181887
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC3550.030.5168131
GTACACG1401.8515493E-830.2798791
GTACCGC1252.3807479E-730.0547316
TCTGTCG4850.030.0160038
GGTATCA10450.029.7486531
TCAACGC13350.028.8519884
ATCAACG14100.027.653963
CAACGCA14400.026.7481985
AACGCAG15100.026.1237346
GTATCAA16100.025.7452131
GTACATG31200.025.0606981
CTGTCGC5650.024.103639
TACATGG31000.023.807412
TCGCTCT4550.023.7443124
ACATGGG30600.023.6427293
TATCAAC16750.023.5742912
TCTCGCT5000.023.504132
TATACTG5750.022.8735395
TACACGG1452.5299107E-522.6936422
CGCTCTG4450.022.1667735
Produced by FastQC (version 0.11.5)